| Budget Amount *help |
¥100,880,000 (Direct Cost: ¥77,600,000、Indirect Cost: ¥23,280,000)
Fiscal Year 2023: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2022: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2021: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2020: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2019: ¥29,770,000 (Direct Cost: ¥22,900,000、Indirect Cost: ¥6,870,000)
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| Outline of Final Research Achievements |
We have developed a vacuum diffractometer for high-precision data collection on a fixed target and an in-situ measurement system for the generalization of dynamic X-ray crystallography. The vacuum diffractometer was found to be effective for low-background high S/N measurements, as the background was reduced by 99.9% under vacuum conditions compared to atmospheric conditions. The high-temperature humidity controller for the in-situ measurement system was used to control humidity up to 70°C and detect changes in protein structure and hydration structure. In addition, an injector-based microcrystal room temperature serial data collection combining the rtHAG and SS-ROX methods was successfully used for structural analysis. We also succeeded in developing a crystallization method suitable for humidity conditioning measurements to create different crystal systems depending on the scaffold protein.
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