Precise microspectroscopic measurements to complement time-resolved X-ray crystallography

• Project Area: Non-equilibrium-state molecular movies and their applications
• Project/Area Number: 19H05784
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: University of Hyogo
• Principal Investigator: Kubo Minoru 兵庫県立大学, 理学研究科, 教授 (90392878)
• Co-Investigator(Kenkyū-buntansha): 木村 哲就 神戸大学, 理学研究科, 准教授 (70506906) ; 古谷 祐詞 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (80432285)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥142,870,000 (Direct Cost: ¥109,900,000、Indirect Cost: ¥32,970,000); Fiscal Year 2023: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000); Fiscal Year 2022: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000); Fiscal Year 2021: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2020: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000); Fiscal Year 2019: ¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000)
• Keywords: 時間分解分光測定 / 動的構造解析 / 振動分光 / 蛍光分光 / 紫外可視分光 / 時間分解X線結晶構造解析 / 時間分解紫外可視吸収分光 / 時間分解赤外分光 / 時間分解ラマン分光 / 時間分解蛍光分光 / 動的構造生物学 / 分子分光学 / 時間分解計測 / 超精密計測 / 蛋白質 / 生物物理学

Outline of Research at the Start

私たちの体の中では多くの分子が働いており、エネルギー変換、物質輸送、物質代謝などの生命活動を担っています。それらの生体分子は、形を巧みに変えながら働く、謂わば、小さな分子機械です。最近、生体分子が働く様子を動画として観測できる「時間分解Ｘ線構造解析」という手法が開発されました。私たちは、紫外光、可視光、赤外光などさまざまな光を道具に、時間分解Ｘ線構造解析では原理的に観測が難しい、電子レベルの細かな解析を精密に行ないます。時間分解Ｘ線構造解析と相補的に研究を進めながら、生体分子が働くしくみを物理化学的に解明します。

Outline of Final Research Achievements

Proteins work like machines in our bodies by changing their shape. In the MEXT program for Scientific Research on Innovative Areas, "Molecular Movies," the changes in protein shape were recorded as movies using a novel X-ray technique. To complement the movie, we used microspectroscopy to analyze the movements of electrons and protons inside proteins. The results revealed how proteins function at the chemical level. The proteins studied here cover a wide range of biological functions. They include a microbial protein that uses light energy for ion transport, an amphibian protein involved in DNA repair, a fungal protein involved in the nitrogen cycle, and a jellyfish luminescent protein.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、構造生物学分野を化学分野と融合させるものであった。また、タンパク質がはたらく仕組みを化学的に理解することは、今後社会に役立つ人工タンパク質をデザインするイノベイティブな応用研究を展開するうえで基盤を築くものであった。実際に本研究で仕組みを明らかにした光で動作するタンパク質は、光遺伝学（光で神経細胞などの活動を制御する技術）で応用されるものであり、本研究成果はその発展に不可欠な知見を与えた。

Research Products

• [Journal Article] Oxygen-evolving photosystem II structures during S1-S2-S3 transitions (2024)
  • Author(s): Li Hongjie、Nakajima Yoshiki、Nango Eriko、Owada Shigeki、Yamada Daichi、Tosha Takehiko、Tono Kensuke、Joti Yasumasa、Hatsui Takaki、Yabashi Makina、Kubo Minoru、Iwata So、Isobe Hiroshi、Yamaguchi Kizashi、Suga Michihiro、Shen Jian-Ren
  • Journal Title: Nature Volume: 626 Issue: 7999 Pages: 670-677
  • DOI: 10.1038/s41586-023-06987-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Internal Proton Transfer in the Activation of Heliorhodopsin (2024)
  • Author(s): Singh Manish、Hashimoto Masanori、Katayama Kota、Furutani Yuji、Kandori Hideki
  • Journal Title: Journal of Molecular Biology Volume: 436 Issue: 5 Pages: 168273-168273
  • DOI: 10.1016/j.jmb.2023.168273
  • Peer Reviewed
• [Journal Article] Mapping protein dynamics at high spatial resolution with temperature-jump X-ray crystallography (2023)
  • Author(s): Wolff, A. M., Nango, E., Young, I. D., Brewster, A. S., Kubo, M., Nomura, T., Sugahara, M., Owada, S., Barad, B. A., Ito, K., Bhowmick, A., Carbajo, S., Hino, T., Holton, J. M., Im, D., O'Riordan, L. J., Tanaka, T., Tanaka, R., Sierra, R. G., Yumoto, F., Tono, K., Iwata, S., et al
  • Journal Title: Nat. Chem. Volume: 15(11) Issue: 11 Pages: 1549-1558
  • DOI: 10.1038/s41557-023-01329-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Concerted primary proton transfer reactions in a thermophilic rhodopsin studied by time-resolved infrared spectroscopy at high temperature (2023)
  • Author(s): Kuroi Kunisato、Tsukamoto Takashi、Honda Naoya、Sudo Yuki、Furutani Yuji
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1864 Issue: 3 Pages: 148980-148980
  • DOI: 10.1016/j.bbabio.2023.148980
  • Peer Reviewed
• [Journal Article] Covalent Bond between the Lys-255 Residue and the Main Chain Is Responsible for Stable Retinal Chromophore Binding and Sodium-Pumping Activity of Krokinobacter Rhodopsin 2 (2023)
  • Author(s): Ochiai Shoha、Ichikawa Yuki、Tomida Sahoko、Furutani Yuji
  • Journal Title: Biochemistry Volume: 62 Issue: 12 Pages: 1849-1857
  • DOI: 10.1021/acs.biochem.3c00168
  • Peer Reviewed
• [Journal Article] Protonation of Asp116 and distortion of the all-trans retinal chromophore in Krokinobacter eikastus rhodopsin 2 causes a redshift in absorption maximum upon dehydration (2023)
  • Author(s): Tomida Sahoko、Wada Akimori、Furutani Yuji
  • Journal Title: Photochemical & Photobiological Sciences Volume: 22 Issue: 11 Pages: 2499-2517
  • DOI: 10.1007/s43630-023-00464-8
  • Peer Reviewed
• [Journal Article] yntheses, Characterizations, Crystal Structures, and Protonation Reactions of Dinitrogen Chromium Complexes Supported with Triamidoamine Ligands (2023)
  • Author(s): Yoshiaki Kokubo, Kazuki Tsuzuki, Hikari Sugiura, Chiaki Yamamoto, Shunsuke Yomura, Yuko Wasada-Tsutsui, Tomohiro Ozawa, Sachiko Yanagisawa, Minoru Kubo, Shinichi Kugimiya, Hideki Masuda, and Yuji Kajita
  • Journal Title: Inorg. Chem. Volume: 62 Issue: 14 Pages: 5320-5333
  • DOI: 10.1021/acs.inorgchem.2c01561
  • Peer Reviewed
• [Journal Article] Trapping of a Mononitrosyl Nonheme Intermediate of Nitric Oxide Reductase by Cryo-Photolysis of Caged Nitric Oxide (2023)
  • Author(s): Takeda Hanae、Shimba Kanji、Horitani Masaki、Kimura Tetsunari、Nomura Takashi、Kubo Minoru、Shiro Yoshitsugu、Tosha Takehiko
  • Journal Title: The Journal of Physical Chemistry B Volume: 127 Issue: 4 Pages: 846-854
  • DOI: 10.1021/acs.jpcb.2c05852
  • Peer Reviewed / Open Access
• [Journal Article] Specific zinc binding to heliorhodopsin (2023)
  • Author(s): Hashimoto, M., Miyagawa, K., Singh, M., Katayama, K., Shoji, M., Furutani, Y., Shigeta, Y., Kandori, H.
  • Journal Title: Physical Chemistry Chemical Physics Volume: 25 Issue: 4 Pages: 3535-3543
  • DOI: 10.1039/d2cp04718g
  • Peer Reviewed / Open Access
• [Journal Article] Ion-transporting mechanism in microbial rhodopsins: Mini-review relating to the session 5 at the 19th International Conference on Retinal Proteins (2023)
  • Author(s): Furutani, Y., Yang, C.-S.
  • Journal Title: Biophysics and Physicobiology Volume: 20 Issue: Supplemental Pages: n/a
  • DOI: 10.2142/biophysico.bppb-v20.s005
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Identifying antibiotics based on structural differences in the conserved allostery from mitochondrial heme-copper oxidases. (2022)
  • Author(s): Nishida Y, Yanagisawa S, Morita R, Shigematsu H, Shinzawa-Itoh K, Yuki H, Ogasawara S, et al
  • Journal Title: Nat Commun. Volume: 13 Issue: 1 Pages: 7591-7591
  • DOI: 10.1038/s41467-022-34771-y
  • Peer Reviewed / Open Access
• [Journal Article] Brown adipose tissue dysfunction promotes heart failure via a trimethylamine N-oxide-dependent mechanism. (2022)
  • Author(s): Yoshida Y, Shimizu I, Oda K, Sasaoka T, Abe M, Sakimura K, Kubota Y, Yoshimura N, Kajimura S, Zuriaga M, Walsh K, Soga T, Minamino T et al.:
  • Journal Title: Scientific Reports, Volume: 12 Issue: 1 Pages: 14883-14883
  • DOI: 10.1038/s41598-022-19245-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Heme-bound tyrosine vibrations in hemoglobin M: Resonance Raman, crystallography, and DFT calculation (2022)
  • Author(s): Nagatomo Shigenori、Shoji Mitsuo、Terada Takuto、Nakatani Kiyoharu、Shigeta Yasuteru、Hirota Shun、Yanagisawa Sachiko、Kubo Minoru、Kitagawa Teizo、Nagai Masako、Ohki Mio、Park Sam-Yong、Shibayama Naoya
  • Journal Title: Biophysical Journal Volume: 121 Issue: 14 Pages: 2767-2780
  • DOI: 10.1016/j.bpj.2022.06.012
  • Peer Reviewed / Open Access
• [Journal Article] 1156.Heme compound II models in chemoselectivity and disproportionation reactions (2022)
  • Author(s): Ranjana Gupta, Xiao-Xi Li, Youngseob Lee, Mi Sook Seo, Yong-Min Lee, Sachiko Yanagisawa, Minoru Kubo, Ritimukta Sarangi, Kyung-Bin Cho, Shunichi Fukuzumi, and Wonwoo Nam
  • Journal Title: Chem. Sci. Volume: - Issue: 19 Pages: 5707-5717
  • DOI: 10.1039/d2sc01232d
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Rhodopsin-bestrophin fusion proteins from unicellular algae form gigantic pentameric ion channels (2022)
  • Author(s): Rozenberg A,Kaczmarczyk I,Matzov D,Vierock J,Nagata T,Sugiura M,Katayama K,Kawasaki Y,Konno M,Nagasaka Y,Aoyama M,Das I,Pahima E,Church J,Adam S,Borin VA,Chazan A,Augustin S,Wietek J,Dine J,Peleg Y,Kawanabe A,Fujiwara Y,Yizhar O,Sheves M,Schapiro I,Furutani Y,Kandori H,Inoue K,Hegemann P,Beja O,Shalev-Benami M.
  • Journal Title: Nature Structural & Molecular Biology Volume: 29 Issue: 6 Pages: 592-603
  • DOI: 10.1038/s41594-022-00783-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Unusual Photoisomerization Pathway in a Near-Infrared Light Absorbing Enzymerhodopsin (2022)
  • Author(s): Sugiura Masahiro、Ishikawa Kazuki、Katayama Kota、Sumii Yuji、Abe-Yoshizumi Rei、Tsunoda Satoshi P.、Furutani Yuji、Shibata Norio、Brown Leonid S.、Kandori Hideki
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 13 Issue: 40 Pages: 9539-9543
  • DOI: 10.1021/acs.jpclett.2c02334
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers. (2022)
  • Author(s): Hosaka, T. et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 119 Issue: 9
  • DOI: 10.1073/pnas.2117433119
  • Peer Reviewed / Open Access
• [Journal Article] A synthetic model for the possible FeIV2(μ-O)2 core of methane monooxygenase intermediate Q derived from a structurally characterized FeIIIFeIV(μ-O)2 complex. (2022)
  • Author(s): Mikata, Y., Aono, Y., Yamamoto, C., Nakayama, H., Matsumoto, A., Kotegawa, F., Harada, M., Katano, H., Kobayashi, Y., Yanagisawa, S., Kubo, M., Kajiwara, A., Kodera, M
  • Journal Title: Inorganic Chemistry Volume: 61 Issue: 2 Pages: 786-790
  • DOI: 10.1021/acs.inorgchem.1c02699
  • Peer Reviewed
• [Journal Article] Revisiting Alkane Hydroxylation with m‐CPBA (mChloroperbenzoic Acid) Catalyzed by Nickel(II) Complexes (2021)
  • Author(s): Shinke, T., Itoh, M., Wada, T., Morimoto, Y., Yanagisawa, S., Sugimoto, H., Kubo, M., Itoh S.
  • Journal Title: Chemistry A European Journal Volume: 27 Issue: 59 Pages: 14730-14737
  • DOI: 10.1002/chem.202102532
  • Peer Reviewed
• [Journal Article] Short-lived intermediate in N2O generation by P450 NO reductase captured by time-resolved IR spectroscopy and XFEL crystallography (2021)
  • Author(s): Nomura, T., Kimura, T., Kanematsu, Y., Yamada, D., Yamashita, K., Hirata, K., Ueno, G., Murakami, H., Hisano, T., Yamagiwa, R., Takeda, H., Gopalasingam, C., Kousaka, R., Yanagisawa, S., Shoji, O., Kumasaka, T., Yamamoto, M., Takano, Y., Sugimoto, H., Tosha, T., Kubo, M., Shiro, Y
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118 Issue: 21
  • DOI: 10.1073/pnas.2101481118
  • Peer Reviewed / Open Access
• [Journal Article] Capturing structural changes of the S1 to S2 transition of photosystem II using time-resolved serial femtosecond crystallography (2021)
  • Author(s): H Li, Y Nakajima, T Nomura, M Sugahara, S Yonekura, SK Chan, T Nakane, T Yamane, Y Umena, M Suzuki, T Masuda, T Motomura, H Naitow, Y Matsuura, T Kimura, K Tono, S Owada, Y Joti, R Tanaka, E Nango, F Akita, M Kubo, S Iwata, JR Shen and M Suga
  • Journal Title: IUCrJ Volume: 8 Issue: 3 Pages: 431-443
  • DOI: 10.1107/s2052252521002177
  • NAID: 120007037379
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Inverse Hydrogen-Bonding Change Between the Protonated Retinal Schiff Base and Water Molecules upon Photoisomerization in Heliorhodopsin 48C12 (2021)
  • Author(s): Tomida Sahoko、Kitagawa Shinya、Kandori Hideki、Furutani Yuji
  • Journal Title: The Journal of Physical Chemistry B Volume: 125 Issue: 30 Pages: 8331-8341
  • DOI: 10.1021/acs.jpcb.1c01907
  • Peer Reviewed / Open Access
• [Journal Article] Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin (2021)
  • Author(s): Oda Kazumasa、Nomura Takashi、Nakane Takanori、Yamashita Keitaro、Inoue Keiichi、Ito Shota、Vierock Johannes、Hirata Kunio、Maturana Andres D、Katayama Kota、( 32 authers )、Kandori Hideki、Hegemann Peter、Iwata So、Kubo Minoru、Nishizawa Tomohiro、Nureki Osamu
  • Journal Title: eLife Volume: 10 Pages: 1-21
  • DOI: 10.7554/elife.62389
  • NAID: 120006996980
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional Assembly of Caenorhabditis elegans Cytochrome b-2 (Cecytb-2) into Phospholipid Bilayer Nanodisc with Enhanced Iron Reductase Activity. (2021)
  • Author(s): Hamed A Abosharaf,Yuki Sakamoto,Aliaa M Radwan,Keisuke Yuzu,Mika Fujimura,Thoria Diab,Tarek M Mohamed,Eri Chatani,Tetsunari Kimura,Motonari Tsubaki
  • Journal Title: Biomolecules Volume: 11 Issue: 1 Pages: 96-96
  • DOI: 10.3390/biom11010096
  • NAID: 120007162571
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Timing of NO Binding and Protonation in the Catalytic Reaction of Bacterial Nitric Oxide Reductase as Established by Time-Resolved Spectroscopy. (2020)
  • Author(s): Hanae Takeda, Tatsunari Kimura, Takashi Nomura, Masaki Horitani, Azusa Yokota, Akiko Matsubayashi, Shoko Ishii, Yoshitsugu Shiro, Minoru Kubo and Takehiko Tosha
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: - Issue: 7 Pages: 825-833
  • DOI: 10.1246/bcsj.20200038
  • NAID: 130007879554
  • Peer Reviewed / Open Access
• [Journal Article] Copper‐oxygen Dynamics in Tyrosinase Mechanism (2020)
  • Author(s): Fujieda Nobutaka、Umakoshi Kyohei、Ochi Yuta、Nishikawa Yosuke、Yanagisawa Sachiko、Kubo Minoru、Kurisu Genji、Itoh Shinobu
  • Journal Title: Angewandte Chemie International Edition Volume: - Issue: 32 Pages: 13385-13390
  • DOI: 10.1002/anie.202004733
  • Peer Reviewed
• [Journal Article] Enhanced redox reactivity of a nonheme iron(V)-oxo complex binding protons. (2020)
  • Author(s): Xue, S.-S., Li, X.-X., Lee, Y.-M., Seo, M. S., Kim, Y., Yanagisawa, S., Kubo, M., Jeon, Y.-K., Kim, W.-S., Sarangi, R., Kim, S. H., Fukuzumi, S., Nam, W.
  • Journal Title: J. Am. Chem. Soc. Volume: 142 Issue: 36 Pages: 15305-15319
  • DOI: 10.1021/jacs.0c05108
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Zinc Binding to Heliorhodopsin (2020)
  • Author(s): Hashimoto Masanori、Katayama Kota、Furutani Yuji、Kandori Hideki
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 11 Issue: 20 Pages: 8604-8609
  • DOI: 10.1021/acs.jpclett.0c02383
  • Peer Reviewed
• [Journal Article] Direct measurements of ferric reductase activity of human 101F6 and its enhancement upon reconstitution into phospholipid bilayer nanodisc (2020)
  • Author(s): El-Behery, M., Fujimura, M., Kimura, T., Tsubaki, M.
  • Journal Title: Biochem. Biophys. Rep. Volume: 21 Pages: 100730-100730
  • DOI: 10.1016/j.bbrep.2020.100730
  • NAID: 120007026374
  • Peer Reviewed / Open Access
• [Journal Article] SACLA時分割結晶構造解析による動的構造生物学研究～酵素反応の可視化に向けた分子動画～. (2020)
  • Author(s): 久保稔
  • Journal Title: 放射光 Volume: 33 Pages: 266-270
  • Peer Reviewed
• [Journal Article] Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals (2020)
  • Author(s): Wolff, A. M., Kubo, M. et al.
  • Journal Title: IUCrJ Volume: 7 Issue: 2 Pages: 306-323
  • DOI: 10.1107/s205225252000072x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Syntheses, characterizations, and crystal Structures of dinitrogen-divanadium complexes bearing triamidoamine ligands. (2020)
  • Author(s): Kokubo, Y., Wasada-Tsutsui, Y., Yomura, S., Yanagisawa, S., Kubo, M., Kugimiya, S., Kajita, Y., Ozawa, T., Masuda, H.
  • Journal Title: Eur. J. Inorg. Chem. Volume: 2020 Issue: 15-16 Pages: 1456-1464
  • DOI: 10.1002/ejic.201901123
  • Peer Reviewed / Open Access
• [Journal Article] Infrared spectroscopic analysis on structural changes around the protonated Schiff base upon retinal isomerization in light-driven sodium pump KR2. (2020)
  • Author(s): S. Tomida, S. Ito, T. Mato, Y. Furutani, K. Inoue, H. Kandori
  • Journal Title: Biochim Biophys Acta Bioenerg. Volume: - Issue: 7 Pages: 148190-148190
  • DOI: 10.1016/j.bbabio.2020.148190
  • Peer Reviewed
• [Journal Article] Oxidative DNA cleavage, formation of μ-1,1-hydroperoxo species, and cytotoxicity of dicopper(II) complex supported by a p-cresol-derived amide-tether ligand (2019)
  • Author(s): Kadoya, Y., Fukui, K., Hata, M., Miyano, R., Hitomi, Y., Yanagisawa, S., Kubo, M., Kodera, M.
  • Journal Title: Inorg. Chem. Volume: 58 Issue: 21 Pages: 14294-14298
  • DOI: 10.1021/acs.inorgchem.9b02093
  • Peer Reviewed
• [Journal Article] Fundamental electron-transfer and proton-coupled electron-transfer properties of Ru(iv)-oxo complexes (2019)
  • Author(s): Hiroaki Kotani, Hinatsu Shimomura, Momoka Horimoto, Tomoya Ishizuka, Yoshihito Shiota, Kazunari Yoshizawa, Sachiko Yanagisawa, Yuka Kawahara-Nakagawa, Minoru Kubo, Takahiko Kojima
  • Journal Title: Dalton Transactions Volume: 48 Issue: 35 Pages: 13154-13161
  • DOI: 10.1039/c9dt02734c
  • NAID: 120007133077
  • Peer Reviewed
• [Journal Article] Pump-Probe Time-Resolved Serial Femtosecond Crystallography at SACLA: Current Status and Data Collection Strategies (2019)
  • Author(s): Nango Eriko、Kubo Minoru、Tono Kensuke、Iwata So
  • Journal Title: Applied Sciences Volume: 9 Issue: 24 Pages: 5505-5505
  • DOI: 10.3390/app9245505
  • Peer Reviewed / Open Access
• [Presentation] Time resolved spectroscopy for monitoring the protein dynamics in microcrystals (2023)
  • Author(s): Kubo Minoru
  • Organizer: UK-Japan meeting on dynamic and time-resolved crystallography 2023
  • Int'l Joint Research / Invited
• [Presentation] Time-resolved IR characterization of the key intermediate in N2O generation by P450 NO reductase (2023)
  • Author(s): Kubo Minoru
  • Organizer: 8th Georgian Bay International Conference on Bioinorganic Chemistry (CANBIC-8)
  • Int'l Joint Research / Invited
• [Presentation] Molecular Mechanisms of Microbial Rhodopsins Studied by Infrared Spectroscopy (2023)
  • Author(s): Furutani Yuji
  • Organizer: 8th Taiwan International Symposium on Raman Spectroscopy (TISRS2023)
  • Int'l Joint Research / Invited
• [Presentation] Development of microfluidic mixers for time-resolved SFX and spectroscopy and their applications (2023)
  • Author(s): Kimura Tetsunari
  • Organizer: UK-Japan meeting on dynamic and time-resolved crystallography 2023
  • Int'l Joint Research / Invited
• [Presentation] 光によるDNA修復のメカニズムと分子進化 (2023)
  • Author(s): 久保稔
  • Organizer: 第1回ATIコンファレンス バイオ単分子研究会
  • Invited
• [Presentation] 時間分解振動分光による酵素中間体の化学構造解析 (2023)
  • Author(s): 久保稔
  • Organizer: 蛋白研セミナー「タンパク質のダイナミクスと機能」
  • Invited
• [Presentation] 分光学から観たDNA光修復のメカニズム (2023)
  • Author(s): 久保稔
  • Organizer: 高速分子動画オンラインセミナー
  • Invited
• [Presentation] シトクロムc酸化酵素のプロトンポンプ活性評価を通したHigd1Aの機能的意義の解明 (2023)
  • Author(s): 佐藤航、柳澤幸子、新澤 -伊藤恭子、西田優也、長尾壮将、新谷泰範、久保稔
  • Organizer: 第49回生体分子科学討論会
• [Presentation] Visible resonance Raman Study to elucidate the action mechanism of CHCHD2 for activating cytochrome oxidase (2023)
  • Author(s): 亀井拓斗、柳澤幸子、島田敦広、Stephanie Gladyck、Siddhesh Aras、Maik Huettemann、Lawrence Glossman、久保稔
  • Organizer: 第61回日本生物物理学会年会
• [Presentation] 赤外分光計測による膜タンパク質のイオン輸送・透過機構の探究 (2023)
  • Author(s): 古谷祐詞
  • Organizer: 第23回日本蛋白質科学会年会ワークショップ「タンパク質の高速分子動画：実験と理論の協奏を目指して」
  • Invited
• [Presentation] 時間分解分光測定によるタンパク質の機能・構造解析 (2023)
  • Author(s): 木村哲就
  • Organizer: 東北大学多元研セミナー
  • Invited
• [Presentation] 酵素反応ダイナミクスの時間分解分光追跡 (2022)
  • Author(s): 久保稔
  • Organizer: 第95回日本生化学会大会 シンポジウム "タンパク質構造ダイナミクス研究の最近のトピックスと未来展望"
  • Invited
• [Presentation] Time-Resolved Spectroscopy for Tracking DNA Repair by Photolyase (2022)
  • Author(s): Kubo, M.
  • Organizer: Molecular Movie and Beyond
  • Int'l Joint Research
• [Presentation] チトクロムc酸化酵素活性増強因子Higd1aの作用機序に関する分光学的研究 (2022)
  • Author(s): 柳澤幸子、松村和香、長尾壮将、西田優也、伊藤新澤恭子、新谷泰範、久保稔
  • Organizer: 第48回生体分子科学討論会
• [Presentation] 楞野亜衣、山田大智、重田育照、山元淳平、久保稔 (2022)
  • Author(s): 時間分解赤外分光法による(6-4)光修復酵素の光修復中間体の解析測定
  • Organizer: 第48回生体分子科学討論会
• [Presentation] The positive regulation mechanism of cytochrome c oxidase by Higd1A in proteoliposome (2022)
  • Author(s): 佐藤航、柳澤幸子、新澤-伊藤恭子、西田優也、長尾壮将、新谷泰範、久保稔
  • Organizer: 第60回日本生物物理学会年会
• [Presentation] Rational design of myoglobin dimers for ligand binding cooperativity (2022)
  • Author(s): 長尾聡、丸尾知紘、山田将司、山田大智、久保稔
  • Organizer: 第60回日本生物物理学会年会
• [Presentation] Structural changes of rhodopsin studied by surface-enhanced infrared spectroscopy as a model system of membrane proteins (2022)
  • Author(s): Furutani, Y.
  • Organizer: 第60回日本生物物理学会年会 シンポジウム "Utilization of Advanced Infrared Sources for Biophysical Studies"
  • Invited
• [Presentation] Microspectroscopic systemsfor time-resolved measurementsof protein microcrystals (2022)
  • Author(s): Kimura, T.
  • Organizer: Molecular Movie and Beyond
  • Int'l Joint Research
• [Presentation] Development and application of complementary spectroscopic methods for dynamic structural biology (2022)
  • Author(s): Kimura, T.
  • Organizer: IPR seminar
  • Int'l Joint Research / Invited
• [Presentation] ヘムABCトランスポーター; BhuUV-T の膜輸送に関するキネティクス解析 (2022)
  • Author(s): 木村哲就
  • Organizer: 第22回日本蛋白質科学会年会
  • Invited
• [Presentation] イクオリン -Ca2+間相互作用の熱力学的解析 (2022)
  • Author(s): 黒木麗、船橋俊也、大西裕介、中津亨、木村哲就
  • Organizer: 第22回日本蛋白質科学会年会
• [Presentation] マイクロ流路デバイスを用いた時間分解顕微分光法による膜輸送の分子機構解析 (2022)
  • Author(s): 木村哲就
  • Organizer: 神戸大学先端融合研究環開拓プロジェクトシンポジウム
  • Invited
• [Presentation] 膜輸送タンパク質 ABC トランスポーターの機能・構造変化の分光学的解析 (2022)
  • Author(s): 木村哲就
  • Organizer: 日本分光学会 関西支部令和３年度講演会
  • Invited
• [Presentation] Time-resolved infrared and XFEL analyses of P450nor reaction intermediates using caged-substrate. (2021)
  • Author(s): Kubo, M.
  • Organizer: Pacifichem2021
  • Int'l Joint Research / Invited
• [Presentation] Combining time-resolved XFEL crystallography with IR spectroscopy for capturing P450nor reaction intermediates. (2021)
  • Author(s): Kubo, M.
  • Organizer: Pacifichem2021
  • Int'l Joint Research / Invited
• [Presentation] 時間分解赤外分光法を用いた(6-4)光修復酵素の光修復中間体の追跡 (2021)
  • Author(s): 楞野亜衣, 山田大智, 山元淳平, 久保稔
  • Organizer: 第47回生体分子科学討論会（優秀ポスター賞受賞）
• [Presentation] Structure of a heme-oxy intermediate of cytochrome P450BM3 catalyzing a non-natural substrate (2021)
  • Author(s): 桑野わ子, 長尾聡, 當舎武彦, Joshua Kyle Stanfield, 笠井千枝, 有安真也, 荘司長三, 杉本宏, 久保稔
  • Organizer: 第59回日本生物物理学会年会（学生発表賞受賞）
• [Presentation] Infrared Spectroscopy for analyzing chemical reactions and protein functions (2021)
  • Author(s): Furutani, Y.
  • Organizer: Webinar on microscopy & microanalysis: towards high impact research series 5
  • Int'l Joint Research / Invited
• [Presentation] 時間分解赤外分光法から見たタンパク質の動的構造変化 (2021)
  • Author(s): 古谷祐詞
  • Organizer: 第94回日本生化学会大会
  • Invited
• [Presentation] Surface-enhanced infrared spectroscopy on heliorhodopsin (2021)
  • Author(s): Furutani, Y.
  • Organizer: Pacifichem2021
  • Int'l Joint Research
• [Presentation] Time-resolved spectroscopic analysis on structural changes during the transportation process of sodium pump rhodopsin KR2 (2021)
  • Author(s): Tomida, S., Wada, A., Kandori, H., Furutani, Y.
  • Organizer: Pacifichem2021
  • Int'l Joint Research
• [Presentation] 時間分解分光法を用いたヘムABCトランスポーターBhuUV-Tにおける輸送過程の速度論的解析 (2021)
  • Author(s): 木村哲就
  • Organizer: 第59回日本生物物理学会年会
  • Invited
• [Presentation] ナノディスク再構成型BhuUV-Tによる段階的な基質輸送の分光学的観察 (2021)
  • Author(s): 木村哲就, 浅田拓也, 林沙英, 鍔木基成, 城宜嗣, 杉本宏
  • Organizer: 第21回日本蛋白質科学会年会
  • Invited
• [Presentation] Developments and applications of time-resolved microspectroscopy for protein micro-crystals (2021)
  • Author(s): Kimura, T.
  • Organizer: Pacifichem2021
  • Int'l Joint Research
• [Presentation] Transmembrane electron-transfer reaction of 101F6 protein reconstituted into nanodisc (2021)
  • Author(s): Yamaguchi, A., Abosharaf, H., Tsubaki, M., Kimura, T.
  • Organizer: Pacifichem2021
  • Int'l Joint Research
• [Presentation] Structural changes of a-synuclein, an intrinsically disordered protein, along the lipid-binding and oligomerization revealed by fluorescence lifetime measurements (2021)
  • Author(s): Sasada, K., Kimura, T., Matsubara, R., Fujii, K.
  • Organizer: Pacifichem2021
  • Int'l Joint Research
• [Presentation] 光駆動イオン輸送タンパク質による光・電気エネルギー変換システムの構築 (2020)
  • Author(s): 古谷祐詞
  • Organizer: シンポジウム「社会実装を見据えた微生物・生体関連技術の最前線」, オンデマンドWeb開催（名古屋工業大学）
  • Invited
• [Presentation] Structural and functional dynamics of ABC transporters clarified by novel time-resolved measurement systems (2020)
  • Author(s): Kimura, T.
  • Organizer: International Conference on Chemical and Environmental Sciences 2020
  • Int'l Joint Research / Invited
• [Presentation] 高速分子動画撮影を補完する時間分解分光測定法の開発と微結晶タンパク質試料への応用 (2020)
  • Author(s): 木村 哲就
  • Organizer: 第93回生化学会大会
  • Invited
• [Presentation] 分子拡散混合を利用したヘムABCトランスポーターの時間分解分光解析 (2020)
  • Author(s): 木村 哲就
  • Organizer: 第2回ピコバイオロジー研究会
  • Invited
• [Presentation] XFEL結晶構造解析と振動分光法を用いたタンパク質の動的精密構造解析 (2019)
  • Author(s): 久保稔
  • Organizer: 鳥取大学GSCセミナー，鳥取大学工学部附属GSC研究センター（鳥取県・鳥取市），2019年9月27日．
  • Invited
• [Presentation] タンパク質の動的構造と機能 (2019)
  • Author(s): 久保稔
  • Organizer: 第46回生体分子科学討論会，筑波大学大学会館（茨城県つくば市），2019年6月22日．
  • Invited
• [Presentation] Membrane Protein Dynamics Studied by Time-resolved Measurements (2019)
  • Author(s): Kimura, T.
  • Organizer: Indo-Japan Joint Workshop “Frontiers in Molecular Spectroscopy: From Fundamentals to Applications in Chemistry and Biology” Nov. 1, 2019, Kobe (Japan).
  • Int'l Joint Research / Invited
• [Presentation] 赤外分光法によるイオンチャネルタンパク質におけるイオン-タンパク質間相互作用の研究 (2019)
  • Author(s): 古谷祐詞
  • Organizer: ISSPワークショップ「レチナールタンパク質の光機能発現の物理と化学」プログラム, 東京大学物性研究所（千葉県・柏市）, 2019年9月5日．
• [Presentation] Time-resolved FTIR spectroscopy for studying molecular mechanisms of sodium and lithium ion transportation of Krokinobacter rhodopsin 2 (2019)
  • Author(s): Tomida, S., Kandori, H., Furutani, Y.
  • Organizer: The 57th Annual Meeting of the Biophysical Society of Japan, Sep. 24-26, 2019, Seagaia Convention Center (Miyazaki, Miyazaki).
• [Presentation] Characterization of Antarctic inward proton pumping microbial rhodopsins (AntRs) (2019)
  • Author(s): Harris, A., Tomida, S., Schubert, L., Lazaratos, M., Watt, E., Hoang, A., Bondar, A.-N., Heberle, J., Furutani, Y., Kandori, H., Brown, L.
  • Organizer: The 57th Annual Meeting of the Biophysical Society of Japan, Sep. 24-26, 2019, Seagaia Convention Center (Miyazaki, Miyazaki).
• [Presentation] Structural features of enzyme rhodopsins revealed by infrared spectroscopy (2019)
  • Author(s): Watari, M., Ikuta, T., Hutamata, H., Yamada, D., Shihoya, W., Yoshida, K., Furutani, Y., Tsunoda, S., Nureki, O., Kandori, H.
  • Organizer: The 57th Annual Meeting of the Biophysical Society of Japan, Sep. 24-26, 2019, Seagaia Convention Center (Miyazaki, Miyazaki).
• [Book] タンパク質の構造解析手法とIn silicoスクリーニングの応用事例（第1章 第6節「ラマン分光法の構造生物学的利用」分担執筆） (2023)
  • Author(s): 久保稔, 山田大智
  • Total Pages: 530
  • Publisher: 技術情報協会
  • ISBN: 9784861049958
• [Book] ヘムタンパク質の科学（第1編 第5章 第2節 「反応ダイナミクスの時間分解測定」分担執筆） (2022)
  • Author(s): 久保稔（城宜嗣、青野重利、齋藤正男 監修）
  • Total Pages: 472
  • Publisher: エヌ・ティー・エス
  • ISBN: 9784860437787
• [Book] ヘムタンパク質の科学（第1編 第5章 第5節 「反応速度論」分担執筆） (2022)
  • Author(s): 當舎 武彦、木村 哲就（城宜嗣、青野重利、齋藤正男 監修）
  • Total Pages: 472
  • Publisher: エヌ・ティー・エス
  • ISBN: 9784860437787
• [Book] 膜タンパク質工学ハンドブック（分担執筆：第1編 第1章 X線自由電子レーザーによる膜タンパク質の構造解析） (2020)
  • Author(s): 溝端栄一, 久保稔
  • Total Pages: 538
  • Publisher: (株)エヌ・ティー・エス
  • ISBN: 9784860435370
• [Book] Optogenetics. Light-Sensing Proteins and Their Applications in Neuroscience and Beyond Advances in Experimental Medicine and Biology (分担執筆：Part I 5. Optogenetic Modulation of Ion Channels by Photoreceptive Proteins) (2020)
  • Author(s): Hisao Tsukamoto and Yuji Furutani
  • Total Pages: 663
  • Publisher: Springer Nature Singapore Pte Ltd.
  • ISBN: 9789811587627
• [Remarks] 名古屋工業大学 古谷研究室ホームページ
  • URL: http://biophys-molsci.web.nitech.ac.jp/index.html
• [Patent(Industrial Property Rights)] 膜タンパク質の安定化のための液体組成物および方法 (2023)
  • Inventor(s): 藤田恭子, 溝端栄一, 古谷祐詞
  • Industrial Property Rights Holder: 藤田恭子, 溝端栄一, 古谷祐詞
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2023-030067
  • Filing Date: 2023