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Comprehensive research of signal transduction of cell

Planned Research

Project AreaInformation physics of living matters
Project/Area Number 19H05797
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionOsaka University

Principal Investigator

test test  大阪大学, 大学院生命機能研究科, 教授 (80301216)

Co-Investigator(Kenkyū-buntansha) 福岡 創  大阪大学, 大学院生命機能研究科, 准教授 (50447190)
蔡 栄淑  大阪大学, 大学院生命機能研究科, 助教 (40378716)
Project Period (FY) 2019-06-28 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥108,810,000 (Direct Cost: ¥83,700,000、Indirect Cost: ¥25,110,000)
Fiscal Year 2023: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2022: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2021: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2020: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2019: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Keywords走化性
Outline of Research at the Start

大腸菌走化性システムは、外界の誘因・忌避物質が細胞極に存在する受容体に結合し、その情報は細胞内を通して各モーターに伝わり、モーターの回転方向が転換される。申請者は、走化性刺激受容において、協同性、適応性、自発性の3つの特徴が、受容体のメチル化レベルの変動という共通の分子機構で実現されている、という従来説とは全く異なる新しいモデルを提唱している。そこで、1細胞レベルでの走化性動態を、活性を保ったまま明らかにする。分子レベルの反応ネットワークと細胞レベルの応答をつなぐ格好のモデル系であり、領域内の理論グループとの共同研究体制を構築することにより実験と理論との有機的な融合を目指す。

Outline of Final Research Achievements

In this study, we focused on the bacterial repellent response and measured the localization of CheB to the cell poles, which is necessary for the adaptive phenomenon. We simultaneously measured the change in fluorescence intensity at the poles and the motor rotation after the administration of repellent substances, and estimated the dependence on the concentration of repellent substances, the correlation between the rotation and the localization, and the number of localized molecules. Furthermore, the response of mutants in which the CheB binding site of the receptor was deleted was also measured. In addition, we developed a system to simultaneously measure and analyze the responses of multiple bacteria to eliminate the variation among experiments. As a result, the variability was clearly reduced compared to individual measurements. We are currently investigating whether this remaining variability is unique to E. coli or to the experimental conditions.

Academic Significance and Societal Importance of the Research Achievements

忌避応答に対する応答は,誘因応答と呼ばれ,よりよい環境を目指す応答であり,今までの走化性応答のほとんどの研究は誘因応答であった.しかしながら,忌避応答は,単に誘因応答の逆反応ととらえられてきた.しかしながら,生体の生存に必須なのはむしろ忌避応答であり,忌避応答の欠如は,死,に直結する.本研究においては,忌避応答のメカニズムを回転と局在の同時計測による定量的な研究の第一歩となる.

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (43 results)

All 2024 2023 2022 2021 2020 2019

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (40 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] The Chemoreceptor Sensory Adaptation System Produces Coordinated Reversals of the Flagellar Motors on an Escherichia coli Cell2022

    • Author(s)
      Yumiko Uchida, Tatsuki Hamamoto, Yong-Suk Che, Hiroto Takahashi, John S. Parkinson, Akihiko Ishijima, Hajime Fukuoka
    • Journal Title

      Journal of Bacteriology

      Volume: 204 Issue: 12

    • DOI

      10.1128/jb.00278-22

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Stator Dynamics Depending on Sodium Concentration in Sodium-Driven Bacterial Flagellar Motors2021

    • Author(s)
      Lin Tsai-Shun、Kojima Seiji、Fukuoka Hajime、Ishijima Akihiko、Homma Michio、Lo Chien-Jung
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 765739-765739

    • DOI

      10.3389/fmicb.2021.765739

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli2020

    • Author(s)
      Che Yong-Suk, Sagawa Takashi, Inoue Yuichi, Takahashi Hiroto, Hamamoto Tatsuki, Ishijima Akihiko, Fukuoka Hajime
    • Journal Title

      BIOMOLECULES

      Volume: 10 Issue: 11 Pages: 1544-1544

    • DOI

      10.3390/biom10111544

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸菌べん毛モーターの回転ゆらぎ2024

    • Author(s)
      内田 裕美子
    • Organizer
      生体運動研究合同班会議2024
    • Related Report
      2023 Annual Research Report
  • [Presentation] CheB-GFPの1細胞動態計測による大腸菌セリン受容体Tsrの忌避刺激認識および情報伝達の解析2024

    • Author(s)
      福岡 創
    • Organizer
      生体運動研究合同班会議2024
    • Related Report
      2023 Annual Research Report
  • [Presentation] 大腸菌走化性キメラ受容体での忌避応答2024

    • Author(s)
      内田 裕美子
    • Organizer
      第8回領域会議・終了報告会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 大腸菌べん毛の回転方向に依存した揺らぎとフックの関係2024

    • Author(s)
      福岡 創
    • Organizer
      2023年度べん毛研究交流会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 大腸菌走化性キメラ受容体での忌避応答2024

    • Author(s)
      内田 裕美子
    • Organizer
      2023年度べん毛研究交流会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 計画班成果発表2024

    • Author(s)
      石島 秋彦
    • Organizer
      第8回領域会議・終了報告会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 大腸菌べん毛の回転方向に依存した回転ゆらぎの解析2024

    • Author(s)
      福岡 創
    • Organizer
      第8回領域会議・終了報告会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 適応現象解明のための走化性タンパク質の細胞内動態とべん毛モーター回転の同時計測2023

    • Author(s)
      内田 裕美子
    • Organizer
      第7回領域会議
    • Related Report
      2023 Annual Research Report
  • [Presentation] デコンボリューション法を用いた大腸菌走化性受容体の三次元観察2023

    • Author(s)
      内田 裕美子
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 大腸菌単一細胞における走化性応答時の走化性タンパク質の細胞内動態の観察2023

    • Author(s)
      福岡 創
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 適応を担う2種類の酵素であるCheRとCheBの細胞内動態の比較2023

    • Author(s)
      大島 健登
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 回転方向に依存した大腸菌べん毛モーターの回転ゆらぎ2023

    • Author(s)
      宮本 大聖
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Exploration of isoleucine recognition sites in chemoreceptor using chimeric receptors2023

    • Author(s)
      河原 進之介
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 同種の受容体からなる受容体アレイがもたらす短い適応時間2023

    • Author(s)
      植田 早紀
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 大腸菌走化性タンパク質の細胞内動態と細胞応答の相関関係2023

    • Author(s)
      福岡 創
    • Organizer
      第7回領域会議
    • Related Report
      2023 Annual Research Report
  • [Presentation] 回転方向に依存した大腸菌べん毛モーターの回転揺らぎの原因2022

    • Author(s)
      Kazumi Akahoshi, Yumiko Uchida, Yong-Suk Che, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      日本生物物理学会 第60回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 1 細胞内でのCheB 局在変化による忌避応答および適応2022

    • Author(s)
      Taiga Deguchi, Yumiko Uchida, Yong-Suk Che, Akihiko Ishijima, Tatsuki Hamamoto, Hajime Fukuoka
    • Organizer
      日本生物物理学会 第60回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] CheZ 局在の大腸菌走化性に及ぼす影響のキャピラリーアッセイによる解析2022

    • Author(s)
      Sawako Matsuda, Yong-Suk Che, Akihiko Ishijima, Masaru Kojima, Hajime Fukuoka
    • Organizer
      日本生物物理学会 第60回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] CheB の極性局在を利用した異種走化性受容体の忌避刺激に対する応答性の比較2022

    • Author(s)
      Shinnosuke Kawahara, Yumiko Uchida, Yong-Suk Che, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      日本生物物理学会 第60回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 大腸菌べん毛モーター間の回転方向転換同調を阻害する走化性受容体クラスター内における野 生型/変異体比率の見積もり2022

    • Author(s)
      Yumiko Uchida, Hajime Fukuoka, Akihiko Ishijima, Yong-Suk Che
    • Organizer
      日本生物物理学会 第60回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 回転する大腸菌べん毛モーター中のGFP-FliL 局在の定量解析2022

    • Author(s)
      Miyuto Miyazaki, Yumiko Uchida, Hajime Fukuoka, Akihiko Ishijima, Yong-Suk Che
    • Organizer
      日本生物物理学会 第60回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] FRET 計測系を用いた低濃度セリンに対する単一大腸菌受容体の協同作用による2 種類の適応2022

    • Author(s)
      Yuki Takada, Akihiko Ishijima, Hajime Fukuoka, Yong-Suk Che
    • Organizer
      日本生物物理学会 第60回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Observation of behavior of flagella in Escherichia coli by using fluorescence staining2021

    • Author(s)
      Tatsuya Nakaue, Yong-Suk Che, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      The 59th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2021 Annual Research Report
  • [Presentation] Change in methylation level in receptor array causes coordinated reversal of flagellar motors on a single Escherichia coli cell2021

    • Author(s)
      Yumiko Uchida , Tatsuki Hamamoto , Yong-Suk Che , Akihiko Ishijima , Hajime Fukuoka
    • Organizer
      The 59th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2021 Annual Research Report
  • [Presentation] Quantification for mutant/WT receptors ratio that collapses receptor cooperativity and switching coordination between flagellar motors2021

    • Author(s)
      Shiori Awa, Yumiko Uchida, Hajime Fukuoka, Akihiko Ishijima, Yong-Suk Che
    • Organizer
      The 59th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2021 Annual Research Report
  • [Presentation] High temporal observation of CheY-binding and dissociation during rotational switching of a single flagellar motor2021

    • Author(s)
      Taro Yuri, Yumiko Uchida, Yong-Suk Che, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      The 59th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2021 Annual Research Report
  • [Presentation] Simultaneous measurement for the stator-incorporation and the flagellar motor rotation2021

    • Author(s)
      Tomohiro Teshima, Yumiko Uchida, Yong-Suk Che, Akihiko Ishijima, Hajime Hukuoka
    • Organizer
      The 59th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2021 Annual Research Report
  • [Presentation] Simultaneous observation of chemotactic response and intracellular behavior of chemotaxis proteins at single E. coli cell.2020

    • Author(s)
      Taro Yuri, Takuma Nakagawa, Keisuke Nishitani, Yong-Suk Che, Yumiko Uchida, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      The 58th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Simultaneous measurement of flagellar motor rotation and Dynamics of CheB localization during chemotactic response2020

    • Author(s)
      Keisuke Nishitani, Tatsuki Hamamoto, Yong-Suk Che, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      The 58th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Elucidation of mechanism for adaptation system through the simultaneous observation CheYp by single cell FRET and flagellar motor rotation2020

    • Author(s)
      Takuma Nakagawa, Tatsuya Yamakoshi, Che Yong-Suk, Hajime Fukuoka, Akihiko Ishijima
    • Organizer
      The 58th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] High temporal resolution measurement of rotational fluctuation of flagellar motor depending on rotational direction.2020

    • Author(s)
      Koki Murai, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      The 58th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Requirement for Chemotaxis Protein CheR and CheB for the switching coordination between two flagellar motors on E. coli cell.2020

    • Author(s)
      Tatsuki Hamamoto, Yumiko Uchida, Yong-Suk Che, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      The 58th Annual Meeting of the Biophysics Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] The Chemotactic Response and Motor Function of a Bacterial Flagellar Motor in a Single Cell2020

    • Author(s)
      石島秋彦
    • Organizer
      Sensory Transduction in Microorganisms, Gordon Research Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 大腸菌走化性応答と情報伝達の蛍光イメージング2020

    • Author(s)
      福岡 創,山越 達矢,西谷 恵輔,蔡 栄淑,石島 秋彦
    • Organizer
      2020年生体運動研究合同班会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] Simultaneous measurement of flagellar motor rotation and CheYp concentration via single cell FRET2019

    • Author(s)
      Tatsuya Yamakoshi, Yong-Suk Che, Akihiko Ishijima, Hajime Fukuoka
    • Organizer
      第57回日本生物物理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Developing the detection system of the conformational change in rotating flagellar motor by single motor FRET2019

    • Author(s)
      Takuma Nakagawa 1, Tatsuya Yamakoshi, Yong-Suk Che, Akihiko Ishijima, Hajikme Fukuoka
    • Organizer
      第57回日本生物物理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 1細胞FRETによるべん毛モーター回転と情報伝達分子濃度の同時計測2019

    • Author(s)
      福岡 創
    • Organizer
      「情報物理学でひもとく生命の秩序と設計原理」第1回領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] FRETによる回転する単一べん毛モーター構造変化の検出系の開発2019

    • Author(s)
      中川拓真
    • Organizer
      「情報物理学でひもとく生命の秩序と設計原理」第1回領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 走化性の適応現象を担うCheBの細胞内動態と適応現象の1細胞同時計測2019

    • Author(s)
      西谷恵輔
    • Organizer
      「情報物理学でひもとく生命の秩序と設計原理」第1回領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高速カメラを用いたべん毛モーター回転の高時間分解能計測2019

    • Author(s)
      村井航希
    • Organizer
      「情報物理学でひもとく生命の秩序と設計原理」第1回領域会議
    • Related Report
      2019 Annual Research Report

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Published: 2019-07-04   Modified: 2025-01-30  

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