Epigenome formation and cellular differentiation in mouse embryogenesis

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062003
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: TANAKA Satoshi 東京大学, 大学院・農学生命科学研究科, 准教授 (90242164)
• Co-Investigator(Kenkyū-buntansha): OHGANE Jun 東京大学, 大学院・農学生命科学研究科, 助教 (50313078)
• Co-Investigator(Renkei-kenkyūsha): SHIOTA Kunio 東京大学, 大学院・農学生命科学研究科, 教授 (80196352)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥63,000,000 (Direct Cost: ¥63,000,000); Fiscal Year 2012: ¥12,600,000 (Direct Cost: ¥12,600,000); Fiscal Year 2011: ¥12,600,000 (Direct Cost: ¥12,600,000); Fiscal Year 2010: ¥12,600,000 (Direct Cost: ¥12,600,000); Fiscal Year 2009: ¥12,600,000 (Direct Cost: ¥12,600,000); Fiscal Year 2008: ¥12,600,000 (Direct Cost: ¥12,600,000)
• Keywords: DNAメチル化 / マウス / 胚盤胞 / 内部細胞塊 / 栄養外胚葉 / 栄養膜細胞 / 胎盤 / 初期胚 / 幹細胞 / クローン / 胚性幹細胞 / 栄養膜幹細胞 / 栄養芽層

Research Abstract

We analyzed DNA methylation profiles of mouse embryonic stem cells (ESCs) and trophoblast stem cells (TSCs), and compared those with DNA methylation profiles of some somatic tissues. Consequently, we identified genomic regions that show methylation statuses specific to each stem cell line. By using DNA methylation profile as one of the indexes, we revealed that the TSCs derived from blastocysts produced by somatic cell nuclear transfer are indistinguishable from normal TSCs. We also showed that trophoblast-specific DNA methylation occurs after the segregation of the trophectoderm and inner cell mass in the mouse periimplantation embryo.

Research Products

• [Journal Article] Trophoblast-specific DNA methylation occurs after the segregation of the trophectoderm and inner cell mass in the mouse periimplantation embryo (2012)
  • Author(s): Nakanishi, M., Hayakawa, K., Nakabayashi, K., Hata. K., Shiota, K., and Tanaka, S
  • Journal Title: Epigenetics Volume: 71 Pages: 173-182
  • Peer Reviewed
• [Journal Article] Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression (2012)
  • Author(s): Okae, H., Hiura, H., Nishida, Y., Funayama, R., Tanaka, S., Chiba, H., Yaegashi, N., Nakayama, K., Sasaki, H,, and Arima, T
  • Journal Title: Hum Mol Genet Volume: 21 Pages: 548-558
  • Peer Reviewed
• [Journal Article] Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression (2012)
  • Author(s): Hiroaki Okae, et al
  • Journal Title: Human Molecular Genetics Volume: 21 Issue: 3 Pages: 548-558
  • DOI: 10.1093/hmg/ddr488
  • Peer Reviewed
• [Journal Article] 栄養膜幹細胞 (2011)
  • Author(s): 中西もも,田中智
  • Journal Title: 臨床婦人科産科 Volume: 65 Pages: 260-265
• [Journal Article] Non-CpG methylation occurs in the regulatory region of Sry gene (2011)
  • Author(s): Nishino, K., Hattori, N. Sato, S., Arai, Y., Tanaka, S., Nagy, A., and Shiota, K
  • Journal Title: J. Reprod. Dev Volume: 57 Pages: 586-593
  • NAID: 10029878227
  • Peer Reviewed
• [Journal Article] DNA Methylation-dependent modulator of Gsg2/Haspin gene expression (2011)
  • Author(s): Sato, S., Maeda, C., Hattori, N., Yagi, S., Tanaka, S., and Shiota, K
  • Journal Title: J. Reprod. Dev Volume: 57 Pages: 526-533
  • NAID: 10029593421
  • Peer Reviewed
• [Journal Article] DNA Methylation-dependent Modulator of Gsg2/Haspin Gene Expression (2011)
  • Author(s): Shun Sato, et al
  • Journal Title: Journal of Reproduction and Development Volume: 57 Issue: 4 Pages: 526-533
  • DOI: 10.1262/jrd.11-031A
  • NAID: 10029593421
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Non-CpG Methylation Occurs in the Regulatory Region of the Sry Gene (2011)
  • Author(s): Koichiro Nishino, et al
  • Journal Title: Journal of Reproduction and Development Volume: 57 Issue: 5 Pages: 586-593
  • DOI: 10.1262/jrd.11-033A
  • NAID: 10029878227
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] 栄養膜幹細胞 (2011)
  • Author(s): 中西もも, 田中智
  • Journal Title: 臨味捌揮妥揮 65 Pages: 260-265
• [Journal Article] Enrichment of short interspersed transposable elements to embryonic stem cell-specific hypomethylated gene regions (2010)
  • Author(s): Muramoto, H., Yagi, S., Hirabayashi, K., Sato, S., Ohgane, J., Tanaka, S., and Shiota, K
  • Journal Title: Genes Cells Volume: 15 Pages: 855-865
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) residing in mouse pluripotent stem cells (2010)
  • Author(s): Sato, S., Yagi, S., Arai, Y., Hirabayashi, K., Hattori, N., Iwatani, M., Okita, K., Ohgane, J., Tanaka, S., Wakayama, T., Yamanaka, S., and Shiota, K
  • Journal Title: Genes Cells Volume: 15 Pages: 607-618
  • Peer Reviewed
• [Journal Article] Trophoblast cell lineage in cloned mouse embryos (2010)
  • Author(s): Oda, M., Shiota, K., and Tanaka, S
  • Journal Title: Dev. Growth Differ Volume: 52 Pages: 285-291
  • NAID: 10027564250
  • Peer Reviewed
• [Journal Article] Interplay between DNA methylation, histone modification and chromatin remodeling in stem cells and during development (2010)
  • Author(s): Ikegami, K., Ohgane, J., Tanaka, S., Yagi, S., and Shiota, K
  • Journal Title: Int. J. Dev. Biol Volume: 53 Pages: 203-214
  • Peer Reviewed
• [Journal Article] Resistance to 5-aza-2'-deoxycytidine in genic regions compared to non-genic repetitive sequences (2010)
  • Author(s): Lim, H.W., Iwatani, M., Hattori, N., Tanaka, S., Yagi, S., and Shiota, K
  • Journal Title: J. Reprod. Dev Volume: 56 Pages: 86-93
  • NAID: 10026323611
  • Peer Reviewed
• [Journal Article] Trophoblast cell lineage in cloned mouse embryos (2010)
  • Author(s): Oda, M., Shiota, K., Tanaka, S.
  • Journal Title: Dev.Growth Differ 52 Pages: 285-291
  • NAID: 10027564250
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) residing in mouse pluripotent stem cells (2010)
  • Author(s): Sato, S., Yagi, S., 他10名(9番目)
  • Journal Title: Genes Cells 15 Pages: 607-618
  • Peer Reviewed
• [Journal Article] Enrichment of short interspersed transposable elements to embryonic stem cell-specific hypomethylated gene regions. (2010)
  • Author(s): Hiroki Muramoto, et al.
  • Journal Title: Genes to Cells Volume: 15 Pages: 855-865
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions(T-DMRs)residing in mouse pluripotent stem cells (2010)
  • Author(s): Shinya Sato, et al.
  • Journal Title: Genes to Cells(受理済) (印刷中)
  • Peer Reviewed
• [Journal Article] Trophoblast cell lineage in cloned mouse embryos.(総説) (2010)
  • Author(s): Mayumi Oda, et al.
  • Journal Title: Development, Growth & Differentiation 52 Pages: 285-291
  • Peer Reviewed
• [Journal Article] Resistance to 5-aza-2'-deoxycytidine in Genic Regions Compared to Non-genic Repetitive Sequences (2010)
  • Author(s): Hui Wen Lim, et al.
  • Journal Title: Journal of Reproduction and Development 56 Pages: 86-93
  • Peer Reviewed
• [Journal Article] Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos (2009)
  • Author(s): Oda, M., Tanaka, S., Yamazaki, Y., Ohta, H., Iwatani, M., Suzuki, M., Ohgane, J., Hattori, N., Yanagimachi, R., Wakayama, T., and Shiota, K. (OM and TS contributed equally)
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Pages: 16293-16297
  • Peer Reviewed
• [Journal Article] Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos (2009)
  • Author(s): Oda, M., Tanaka, S., (equal contribution), 他9名
  • Journal Title: Proc. Nat.Acad.Sci.USA 106 Pages: 16293-16297
  • Peer Reviewed
• [Journal Article] Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos (2009)
  • Author(s): Mayumi Oda, et al.
  • Journal Title: Proceedings of the National Academy of Science, USA 106 Pages: 16293-16297
  • Peer Reviewed
• [Journal Article] Interplay between DNA methylation, histone modification and chromatin remodeling in stem cells and during development.(総説) (2009)
  • Author(s): Kohta Ikegami, et al.
  • Journal Title: International Journal of Developmental Biology 53 Pages: 203-214
  • Peer Reviewed
• [Journal Article] DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression (2008)
  • Author(s): Yagi, S., Hirabayashi, K., Sato, S., Li, W., Takahashi, Y., Hirakawa, T., Wu, G., Hattori, N., Hattori, N., Ohgane, J., Tanaka, S., Liu, X.S., and Shiota, K
  • Journal Title: Genome Res Volume: 18 Pages: 1969-1978
  • Peer Reviewed
• [Journal Article] DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression (2008)
  • Author(s): Yagi, S., Hirabayashi, K., 他11名(11番目)
  • Journal Title: Genome Res 18 Pages: 1969-1978
  • Peer Reviewed
• [Journal Article] DNA methylation profile of tissuedependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific (2008)
  • Author(s): Shintaro Yagi et.al.
  • Journal Title: Genome Research 18 Pages: 1969-1978
  • Peer Reviewed
• [Journal Article] Isolation and manipulation of mouse trophoblast stem cells (2008)
  • Author(s): Emi Himeno et.al.
  • Journal Title: Current Protocols in Stem Cell Biology
• [Presentation] Trophoblast-specific DNA methylation occurs after the segregation of trophectoderm and inner cell mass in mouse preimplantation embryo (2011)
  • Author(s): Nakanishi, M.O., et al.
  • Organizer: The 2nd World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2011-10-10
• [Presentation] Trophoblast-specific DNA methylation occurs after the segregation of trophectoderm and inner cell mass in mouse preimplantation embryo (2011)
  • Author(s): Momo Nakanishi, et al
  • Organizer: The 2^<nd> World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia(ポスター)
  • Year and Date: 2011-10-10
• [Presentation] Difference in the DNA methylation status arises after segregation of trophoblast and embryonic cell lineages (2011)
  • Author(s): Tanaka, S.
  • Organizer: 国際生殖シンポジウム「生殖生物学の革命と展望:体外受精、胚性幹細胞、エピジェネティクスの展望」
  • Place of Presentation: 岩手
  • Year and Date: 2011-09-14
• [Presentation] Difference in the DNA methylation status arises after segregation of trophoblast and embryonic cell lineages (2011)
  • Author(s): Satoshi Tanaka
  • Organizer: 国際生殖シンポジウム「生殖生物学の革命と展望:体外受精、胚性幹細胞、エピジェネティクスの展望」
  • Place of Presentation: いわて県民情報交流センター・アイーナホール(岩手)(招待講演)
  • Year and Date: 2011-09-14
• [Presentation] Trophoblast-specific DNA methylation occurs after the segregation of trophectoderm and inner cell mass in mouse periimplantation embry (2011)
  • Author(s): Nakanishi, M.O., et al.
  • Organizer: 国際生殖シンポジウム「生殖生物学の革命と展望:体外受精、胚性幹細胞、エピジェネティクスの展望」
  • Place of Presentation: 岩手
• [Presentation] Trophoblast-specific DNA methylation occurs after the segregation of trophectoderm and inner cell mass in mouse periimplantation embryo (2011)
  • Author(s): Momo Nakanishi, et al
  • Organizer: 国際生殖シンポジウム「生殖生物学の革命と展望:体外受精、胚性幹細胞、エピジェネティクスの展望」
  • Place of Presentation: いわて県民情報交流センター・アイーナホール(岩手)(ポスター)
• [Presentation] Establishment of cell lineage-specific DNA methylation profile follows the morphological differentiation of trophoblast and embryonic cell lineages (2010)
  • Author(s): Nakanishi, M.O., et al.
  • Organizer: 43rd Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee, USA
  • Year and Date: 2010-07-31
• [Presentation] Establishment of cell lineage-specific DNA methylation profile follows the morphological differentiation of trophoblast and embryonic cell lineages. (2010)
  • Author(s): Momo Nakanishi, et al.
  • Organizer: 43rd Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee(一般口頭)
  • Year and Date: 2010-07-31
• [Presentation] Normalcy of trophoblast stem cells derived from cloned mouse embryos (2010)
  • Author(s): Tanaka, S
  • Organizer: The First SKLRB Symposia on Frontiers in Periimplantation Biology
  • Place of Presentation: 北京
  • Year and Date: 2010-05-12
• [Presentation] Normalcy of trophoblast stem cells derived from cloned mouse embryos. (2010)
  • Author(s): Satoshi Tanaka
  • Organizer: The First SKLRB Symposia on Frontiers in Periimplantation Biology
  • Place of Presentation: 北京(招待講演)
  • Year and Date: 2010-05-12
• [Presentation] Establishment of cell lineage-specific DNA methylation profile follows the morphological differentiation of trophoblast and embryonic cell lineages (2010)
  • Author(s): Nakanishi, M., et al.
  • Organizer: 43rd Annual Meeting of the Society for the Study of Reproduc tion
  • Place of Presentation: Milwaukee(一般口頭)
• [Presentation] Normalcy of trophoblast stem cells derived from cloned mouse embryos (2010)
  • Author(s): Tanaka, S
  • Organizer: The First Sklrb symposia on Frontiers in Perzimplantation Biology
  • Place of Presentation: Beijing(招待講演)
• [Presentation] Is trophoblast cell lineage abnormal in cloned mouse embryos? (2009)
  • Author(s): 田中 智
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-05-31
• [Presentation] Is trophoblast ce111ineage abnormal in cloned mouse embryos (2009)
  • Author(s): 田中智
  • Organizer: 第42回日本発莞生生物学会大会
  • Place of Presentation: 新潟(招待講演)
  • Year and Date: 2009-05-31
• [Presentation] Is trophoblast cell lineage abnormal in cloned mouse embryos? (2009)
  • Author(s): 田中智
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟コンベンションセンター(新潟市)
  • Year and Date: 2009-05-31
• [Presentation] マウスES細胞でのLin28の機能解析 (2008)
  • Author(s): 姫野 絵美,他
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎
• [Presentation] 栄養膜幹細胞の分化に伴うDNAメチル化プロフィール形成 (2008)
  • Author(s): 吉岡 徹哉,他
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡
• [Presentation] マウスプロラクチン遺伝子ファミリーのDNAメチル化解析 (2008)
  • Author(s): 早川 晃司,他
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡
• [Presentation] マウスプロラクチン遺伝子ファミリーのDNAメチル化解析 (2008)
  • Author(s): 早川晃司, ら
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡(一般口頭)
• [Presentation] 栄養膜幹細胞の分化に伴うDNAメチル化プロフィール形成 (2008)
  • Author(s): 吉岡徹也, ら
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡(一般口頭)
• [Presentation] マウスES細胞でのLin28機能解析 (2008)
  • Author(s): 姫野絵美
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎
• [Book] Isolation and manipulation of mouse trophoblast stem cells. Curr. Protoc. Stem Cell Biol. 7 (2008)
  • Author(s): Himeno, E., Tanaka, S., and Kunath, T
• [Book] Curr Protoc Stem Cell Biol (2008)
  • Author(s): Himeno, E., Tanaka, S., Kunath, T.
  • Publisher: Isolation and manipulation of mouse trophoblast stem cells
• [Remarks] 雑誌論文(11)に関する研究科プレスリリース
  • URL: http://www.a.u-tokyo.ac.jp/topics/tanaka090826.html
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/topics/tanakaO90826.html
• [Remarks] Oda, et al., pNAS 106:16293-16297(2009)に関するプレスリリース
  • URL: http://www.a.u-tokyo.ac.jp/topics/tanaka090826.html