Molecular analysis of germinal granules / nuagein the mouse germline

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: NAKATSUJI Norio 京都大学, 物質-細胞統合システム拠点, 教授 (80237312)
• Co-Investigator(Kenkyū-buntansha): CHUMA Shinichiro 京都大学, 再生医科学研究所, 准教授 (20378889) ; 中馬 新一郎 京都大学, 再生医科学研究所, 助教 (20378839)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥100,000,000 (Direct Cost: ¥100,000,000); Fiscal Year 2012: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2011: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2010: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2009: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2008: ¥20,000,000 (Direct Cost: ¥20,000,000)
• Keywords: 発生・分化 / 遺伝学 / 遺伝子 / 動物 / バイオテクノロジー / 発生分化

Research Abstract

We analyzed tudor-domain containing genes, which encode for specific components of mammalian germinal granules. TDRD1 and TDRD9 cooperate with MILI and MIWI2 and function in retrotransposon silencing at RNA and epigenetic levels. Tdrd6 and 7 act in haploid spermiogenesis and establish chromatoid body architecture, which sharecommon properties with processing bodies and stressgranules. We also identified novel molecules that associate with the tudor-piwipathway and analyzed their function in detail

Research Products

• [Journal Article] GPAT2, a Mitochondrial Acyltransferase in piRNA Biogenesis in Germline Stem Cells (2013)
  • Author(s): Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Katsumata A, Nishimura K, Ohtaka M,Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
  • Journal Title: RNA Volume: 19 Pages: 803-810
  • Peer Reviewed
• [Journal Article] GPAT2, a Mitochondrial Acyltransferase, in piRNA Biogenesis in Germline Stem Cells (2013)
  • Author(s): Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Katsumata A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
  • Journal Title: RNA Volume: (in press)
  • Peer Reviewed
• [Journal Article] A Role for Fkbp6 and the Chaperone Machinery in piRNA Amplification and Transposon Silencing (2012)
  • Author(s): Xiol J, Cora E, Koglgruber R, Chuma S, Subramanian S, Hosokawa M, Reuter M, Yang Z, Berninger P, Palencia A, Benes V, Penninger J, Sachidanandam R, Pillai RS
  • Journal Title: Mol Cell Volume: 47 Pages: 970-979
  • Peer Reviewed
• [Journal Article] piRNAs and their involvement in male germline development in mice (2012)
  • Author(s): Pillai RS, Chuma S
  • Journal Title: Dev Growth Differ Volume: 54 Pages: 78-92
  • Peer Reviewed
• [Journal Article] Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing (2012)
  • Author(s): Morozumi Y, Ino R, Takaku M, Hosokawa M, Chuma S, Kurumizaka H
  • Journal Title: Nucleic Acids Res Volume: 40 Pages: 3031-3041
  • Peer Reviewed
• [Journal Article] MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing (2011)
  • Author(s): Reuter M, Berninger P, Chuma S, Shah H, Hosokawa M, Funaya C, Antony C, Sachidanandam R, Pillai RS
  • Journal Title: Nature Volume: 480 Pages: 264-267
  • Peer Reviewed
• [Journal Article] Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis (2011)
  • Author(s): Tanaka T, Hosokawa M, Vagin VV, Reuter M, Hayashi E, Mochizuki AL, Kitamura K, Yamanaka H, Kondoh G, Okawa K, Kuramochi-Miyagawa S, Nakano T, Sachidanandam R, Hannon GJ, Pillai RS, Nakatsuji N, Chuma S
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 10579-10584
  • Peer Reviewed
• [Journal Article] MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mousegermline (2011)
  • Author(s): Watanabe T, Chuma S, Yamamoto Y, Kuramochi-Miyagawa S, Totoki Y, Toyoda A, Hoki Y, Fujiyama A, Shibata T, Sado T, Noce T, Nakano T, Nakatsuji N, Lin H, Sasaki H
  • Journal Title: Dev Cell Volume: 20 Pages: 364-375
  • Peer Reviewed
• [Journal Article] TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice (2011)
  • Author(s): Yabuta Y, Ohta H, Abe T, Kurimoto K, Chuma S, Saitou M
  • Journal Title: J Cell Biol Volume: 192 Pages: 781-795
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2011)
  • Author(s): Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T,Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T
  • Journal Title: Genes Dev Volume: 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing (2011)
  • Author(s): Morozumi Y, et al
  • Journal Title: Nucleic Acids Res Volume: 40 Issue: 7 Pages: 3031-3041
  • DOI: 10.1093/nar/gkr1229
  • Peer Reviewed
• [Journal Article] Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing (2011)
  • Author(s): Reuter M, et al
  • Journal Title: Nature Volume: 480 Issue: 7376 Pages: 264-267
  • DOI: 10.1038/nature10672
  • Peer Reviewed
• [Journal Article] MITOPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline (2011)
  • Author(s): Watanabe T
  • Journal Title: Dev Cell Volume: 20 Pages: 364-375
  • Peer Reviewed
• [Journal Article] Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3 (2010)
  • Author(s): Yamaji M, Tanaka T, Shigeta M, Chuma S, Saga Y, Saitou M
  • Journal Title: Reproduction Volume: 139 Pages: 381-393
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons. (2010)
  • Author(s): Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama A, Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T
  • Journal Title: Genes Dev. 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa S, et al
  • Journal Title: Genes Dev Volume: 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] Retrotransposon silencing by piRNAs: ping-pong players mark their sub-cellular boundaries (2009)
  • Author(s): Chuma S, Pillai RS
  • Journal Title: PLoS Genet Volume: 5
  • Peer Reviewed
• [Journal Article] Phenotypic plasticity of mouse spermatogonial stem cells (2009)
  • Author(s): Morimoto H, Kanatsu-Shinohara M, Takashima S, Chuma S, Nakatsuji N, Takehashi M, Shinohara T
  • Journal Title: PLoS One Volume: 4
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji M, Tanaka T, Hosokawa M, Reuter M, Stark A, Kato Y, Kondoh G, Okawa K, Chujo T, Suzuki T, Hata K, Martin SL, Noce T, Kuramochi-Miyagawa S, Nakano T, Sasaki H, Pillai RS, Nakatsuji N, Chuma S
  • Journal Title: Dev Cell Volume: 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells (2009)
  • Author(s): Kojima K, Kuramochi-Miyagawa S, Chuma S, Tanaka T, Nakatsuji, N, Kimura T, Nakano T
  • Journal Title: Genes Cells Volume: 14 Pages: 1155-1165
  • Peer Reviewed
• [Journal Article] Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members (2009)
  • Author(s): Vagin VV, Wohlschlegel J, Qu J, Jonsson Z, Huang X, Chuma S, Girard A, Sachidanandam R, Hannon GJ, Aravin AA
  • Journal Title: Genes Dev Volume: 23 Pages: 1749-1762
  • Peer Reviewed
• [Journal Article] Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile (2009)
  • Author(s): Reuter M, Chuma S, Tanaka T, Franz T, Stark A, Pillai RS
  • Journal Title: Nat Struct Mol Biol Volume: 16 Pages: 639-646
  • Peer Reviewed
• [Journal Article] In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells (2009)
  • Author(s): Yamauchi K, Hasegawa K, Chuma S, Nakatsuji N, Suemori H
  • Journal Title: PLoS One Volume: 4
  • Peer Reviewed
• [Journal Article] Mili interacts with tudor domain-containing protein 1 in regulating spermatogenesis (2009)
  • Author(s): Wang J, Saxe JP, Tanaka T, Chuma S, Lin H
  • Journal Title: Curr Biol Volume: 19 Pages: 640-644
  • Peer Reviewed
• [Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects (2009)
  • Author(s): Takashima S, Takehashi M, Lee J, Chuma S, Okano M, Hata K, Suetake I, Nakatsuji N, Miyoshi H, Tajima S, Tanaka Y, Toyokuni S, Sasaki H, Kanatsu-Shinohara M, Shinohara T
  • Journal Title: Biol Reprod Volume: 81 Pages: 155-164
  • Peer Reviewed
• [Journal Article] Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline: germinal granules in mammals (2009)
  • Author(s): Chuma S, Hosokawa M, Tanaka T, Nakatsuji N
  • Journal Title: Mol Cell Endocrinol Volume: 306 Pages: 17-23
  • Peer Reviewed
• [Journal Article] Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. (2009)
  • Author(s): Reuter, M, Chuma, S, Tanaka, T, Franz, T, Stark, A, Pillai, RS
  • Journal Title: Nat.Struct.Mol.Biol. 16 Pages: 639-646
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. (2009)
  • Author(s): Shoji, M, Tanaka, T, Hosokawa, M, Reuter, M, Stark, A, Kato, Y, Kondoh, G, Okawa, K, Chujo, T, Suzuki, T, Hata, K, Martin, SL, Noce, T, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Pillai, RS, Nakatsuji, N, Chuma, S
  • Journal Title: Dev.Cell. 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members. (2009)
  • Author(s): Vagin, VV, Wohlschlegel, J, Qu, J, Jonsson, Z, Huang, X, Chuma, S, Girard, A, Sachidanandam, R, Hannon, GJ, Aravin, AA
  • Journal Title: Genes Dev. 23 Pages: 1749-1762
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji, M, et al
  • Journal Title: Dev Cell 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members (2009)
  • Author(s): Vagin, VV, et al
  • Journal Title: Genes Dev 23 Pages: 1749-1762
  • Peer Reviewed
• [Journal Article] Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile (2009)
  • Author(s): Reuter, M, et al
  • Journal Title: Nat Struct Mol Biol 16 Pages: 639-646
  • Peer Reviewed
• [Journal Article] Mili Interacts with Tudor Domain-Containing Protein 1 in Regulating Spermatogenesis (2009)
  • Author(s): Wang, J, et.al.
  • Journal Title: Curr Biol
  • Peer Reviewed
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin (2008)
  • Author(s): Kanatsu-Shinohara M, Takehashi M, Takashima S, Lee J, Morimoto H, Chuma S, Raducanu A, Nakatsuji N, Fassler R, Shinohara T
  • Journal Title: Cell Stem Cell Volume: 3 Pages: 533-542
  • Peer Reviewed
• [Journal Article] Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells (2008)
  • Author(s): Kanatsu-Shinohara M, Kato M, Takehashi M, Morimoto H, Takashima S, Chuma S, Nakatsuji N, Hirabayashi M, Shinohara T
  • Journal Title: Biol Reprod Volume: 79 Pages: 1121-1128
  • Peer Reviewed
• [Journal Article] Long-term culture of male germline stem cells from hamster testes (2008)
  • Author(s): Kanatsu-Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, Horiuchi T, Shinohara T
  • Journal Title: Biol Reprod Volume: 78 Pages: 611-617
  • Peer Reviewed
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin. (2008)
  • Author(s): Kanatsu-Shinohara, M, Takehashi, M, Takashima, S, Lee, J, Morimoto, H, Chuma, S, Raducanu, A, Nakatsuji, N, Fassler, R, Shinohara, T
  • Journal Title: Cell Stem Cell. 3 Pages: 533-542
  • Peer Reviewed
• [Journal Article] Ultrastructural characterization of spermatogenesis and its evolutionary conservation in the germline : Germinal granules in mammals (2008)
  • Author(s): Chuma, S, et.al.
  • Journal Title: Mol Cell Endocrinol
  • Peer Reviewed
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends on betal-integrin (2008)
  • Author(s): Kanatsu-Shinohara, M, et.al.
  • Journal Title: Cell Stem Cell 3 Pages: 533-542
  • Peer Reviewed
• [Journal Article] piRNA and spermatogenesis in mice
  • Author(s): Chuma S, Nakano T
  • Journal Title: Philos Trans R Soc Lond B Biol Sci Volume: (in press)
  • Peer Reviewed
• [Presentation] piRNAs in the testis : mammalian tudor genes and germinal granules (2013)
  • Author(s): Chuma S
  • Organizer: International Congress of Andrology
  • Place of Presentation: Melbourne, Australia(招待講演)
  • Year and Date: 2013-02-24
• [Presentation] piRNAs in the testis: mammalian tudor genes and germinal granules (2013)
  • Author(s): Chuma S
  • Organizer: International Congress of Andrology
  • Place of Presentation: Australia
• [Presentation] Mammalian tudor related genes in the male germline (2011)
  • Author(s): Chuma S
  • Organizer: 44th meeting of society for the study of reproduction
  • Place of Presentation: Portland, USA
• [Presentation] Germline tudor genes, germinal granules and spermatogenesis in mice (2011)
  • Author(s): Chuma S
  • Organizer: Cold spring harbor asia conference "Developmental Control of Sex, Growth and Cellular Fate"
  • Place of Presentation: Suzhou, China
• [Presentation] Mammalian tudor related genes in the male germline (2011)
  • Author(s): Chuma S
  • Organizer: 44th meeting of society for the study of reproduction
  • Place of Presentation: Portland, USA(招待講演)
• [Presentation] Germline tudor genes, germinal granules and spermatogenesis in mice (2010)
  • Author(s): Shinichiro Chuma
  • Organizer: 特定領域研究国際シンポジウム
  • Place of Presentation: 福岡
• [Presentation] The roles of tudor genes in mammalian spermatogenesis. (2009)
  • Author(s): Takashi Tanaka, Mihoko Hosokawa, Norio Nakatsuji, Shinichiro Chuma
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] The roles of tudor genes in mammalian spermatogenesis (2009)
  • Author(s): Tanaka, T, et al
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Ultrastructural characteristic of spermatogenesis and its evolutionary conservation in the germline (2008)
  • Author(s): Chuma S
  • Organizer: 15th European testis workshop with NAFA annual meeting
  • Place of Presentation: Naantali, Finland
• [Presentation] Protein and RNA assembly of germinal granules/nuage in the germline (2008)
  • Author(s): Chuma S
  • Organizer: World congress on reproductive biology
  • Place of Presentation: Hawaii, USA
• [Presentation] Ultrastructural characteristic of spermatogenesis and its evolutionary conservation in the germline. (2008)
  • Author(s): Shinichiro Chuma
  • Organizer: 15th European testis workshop with NAFA annual meeting
  • Place of Presentation: Naantali, Finland
• [Presentation] Protein and RNA assembly of germinal granules/nuage in the germline. (2008)
  • Author(s): Shinichiro Chuma
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Hawaii, USA
• [Presentation] Mammalian germ cells and germinal granules/nuage. (2008)
  • Author(s): Shinichiro Chuma
  • Organizer: Germ cell-soma interactions in gonadal development and germ cell tumors (workshops " current trends in biomedicine ")
  • Place of Presentation: Baeza, Spain
• [Presentation] Role of TDRD9 in male meiosis and regulatory pathway of transposon RNA and DNA methylation. (2008)
  • Author(s): Shoji, M, Tanaka, T, Hosokawa, M, Kitamura, K, Kato, Y, Kondoh, G, Okawa, K, Chujyo, T, Suzuki, T, Hata, K, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Chuma, S, Nakatsuji, N
  • Organizer: Germ Cells, Cold Spring Habor Laboratory Meeting
  • Place of Presentation: Cold spring harbor, USA
• [Presentation] Role of TDRD9 in male meiosis and regulatory pathway of transposon RNA and DNA methylation (2008)
  • Author(s): Shoji, M, et.al.
  • Organizer: Germ Cells, Cold Spring Habor Laboratory Meeting
  • Place of Presentation: Cold spring harbor, USA
• [Remarks]