Mechanisms of epigenomic regulation in gametogenesis and genomic imprinting

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062010
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University (2010-2012); National Institute of Genetics (2008-2009)
• Principal Investigator: SASAKI Hiroyuki 九州大学, 生体防御医学研究所, 教授 (30183825)
• Co-Investigator(Kenkyū-buntansha): HATA Kenichiro 国立成育医療センター研究所, 周産期病態研究部, 部長 (60360335)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥210,000,000 (Direct Cost: ¥210,000,000); Fiscal Year 2012: ¥42,000,000 (Direct Cost: ¥42,000,000); Fiscal Year 2011: ¥42,000,000 (Direct Cost: ¥42,000,000); Fiscal Year 2010: ¥42,000,000 (Direct Cost: ¥42,000,000); Fiscal Year 2009: ¥42,000,000 (Direct Cost: ¥42,000,000); Fiscal Year 2008: ¥42,000,000 (Direct Cost: ¥42,000,000)
• Keywords: 発生・分化 / 発現制御 / 生殖細胞 / エピゲノム / ゲノム刷込み / ゲノム刷り込み

Research Abstract

In mammalian germ cell development, many epigenetic events including genomic imprinting occur. The changes in the epigenome affect the progression of meiosis and, after fertilization, the developmental potential of the embryo. In this study, we discovered the existence of endogenous siRNA in mouse oocytes, which regulated expression of transposons and genes. We also found that the biogenesis of piRNA in male germ cells requires a phospholipase/nuclease family protein called MitoPLD, and that piRNA is involved in de novo DNA methylation of transposons and a certain imprinted gene(s). Based on the results, we proposed a model in which piRNA-mediated DNA methylation involves nascent noncoding RNA complimentary to the piRNA. Lastly, we developed a method to visualize the DNA methylation status in individual cells.

Research Products

• [Journal Article] Mouse oocyte methylomes at base resolution reveal genome- wide accumulation of non-CpG methylation and role of DNA methyltransferases (2013)
  • Author(s): Shirane K, Toh H, Kobayashi H, Miura F, Chiba H, Ito T, Kono T, Sasaki H
  • Journal Title: PLoS Genet Volume: 9(4) Issue: 4 Pages: 303-306
  • DOI: 10.1371/journal.pgen.1003439
  • Peer Reviewed
• [Journal Article] Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus (2013)
  • Author(s): Yamamoto Y,Watanabe T,Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H
  • Journal Title: Genome Res Volume: 23 Issue: 2 Pages: 292-299
  • DOI: 10.1101/gr.137224.112
  • Peer Reviewed
• [Journal Article] Accumulation and loss of asymmetric non-CpG methylation during male germ-cell development. Nucl (2013)
  • Author(s): Ichiyanagi, T., Ichiyanagi, K., Miyake, M. & Sasaki, H
  • Journal Title: Acids Res Volume: 41 Issue: 2 Pages: 738-745
  • DOI: 10.1093/nar/gks1117
  • Peer Reviewed
• [Journal Article] Mouse oocyte methylomes at base resolution reveal genome-wide accumulation of non-CpG methylation and role of DNA methyltransferases. (2013)
  • Author(s): Shirane, K., et al
  • Journal Title: PLoS Genet. Volume: (in press)
  • Peer Reviewed
• [Journal Article] An atomic model of ZFP57 recognition of CpG methylation within a specific DNA sequence (2012)
  • Author(s): Liu, Y., Toh, H., Sasaki, H., Zhang, X. & Cheng, X
  • Journal Title: Genes Dev Volume: 26 Issue: 21 Pages: 2374-2379
  • DOI: 10.1101/gad.202200.112
  • Peer Reviewed
• [Journal Article] Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice (2012)
  • Author(s): Takahashi, T., Matsuzaki, H., Tomizawa, S., Okamura, E., Ichiyanagi, T., Fukamizu, A., Sasaki, H. & Tanimoto, K
  • Journal Title: Gene Volume: 508 Issue: 1 Pages: 26-34
  • DOI: 10.1016/j.gene.2012.07.062
  • Peer Reviewed
• [Journal Article] Genomic imprinting and its relevance to congenital disease, infertility, molar pregnancy and induced pluripotent stem cells (2012)
  • Author(s): Tomizawa, S. & Sasaki, H
  • Journal Title: J. Hum. Genet Volume: 57 Issue: 2 Pages: 84-91
  • DOI: 10.1038/jhg.2011.151
  • NAID: 10030711407
  • Peer Reviewed
• [Journal Article] Active role of small non-coding RNAs derived from SINE/B1 retrotransposon during early mouse development (2012)
  • Author(s): Ohnishi, Y., Totoki, Y., Toyoda, A., Watanabe, T., Yamamoto, Y., Tokunaga, K., Sakaki, Y., Sasaki, H. & Hohjoh, H
  • Journal Title: Mol. Biol. Rep Volume: 39 Issue: 2 Pages: 903-909
  • DOI: 10.1007/s11033-011-0815-1
  • Peer Reviewed
• [Journal Article] Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression (2012)
  • Author(s): Hiroaki Okae, et al
  • Journal Title: Human Molecular Genetics Volume: 21 Issue: 3 Pages: 548-558
  • DOI: 10.1093/hmg/ddr488
  • Peer Reviewed
• [Journal Article] Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development (2011)
  • Author(s): Ichiyanagi, T., Fukuda, K., Kitayama, J., Yamamoto, Y., Kuramochi-Miyagawa, S., Nakano, T., Yabuta, Y., Seki, Y., Saitou, M., & Sasaki, H
  • Journal Title: Genome Res Volume: 21 Issue: 12 Pages: 2058-2066
  • DOI: 10.1101/gr.123679.111
  • Peer Reviewed
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus (2011)
  • Author(s): Watanabe, T., Tomizawa, S., Mitsuya, K., Totoki, Y., Yamamoto, Y., Kuramochi-Miyagawa, S., Iida, N., Hoki, Y., Murphy, P.J., Toyoda, A., Gotoh, K., Hiura, H., Arima, T., Fujiyama, A., Sado, T., Shibata, T., Nakano, T., Lin, H., Ichiyanagi, K., Soloway, P.D., Sasaki, H
  • Journal Title: Science Volume: 332 Issue: 6031 Pages: 848-52
  • DOI: 10.1126/science.1203919
  • Peer Reviewed
• [Journal Article] Genomic imprinting in mammals: its life cycle, molecular mechanisms and reprogramming (2011)
  • Author(s): Li, Y. & Sasaki, H
  • Journal Title: Cell Res Volume: 21 Issue: 3 Pages: 466-473
  • DOI: 10.1038/cr.2011.15
  • NAID: 120004951578
  • Peer Reviewed
• [Journal Article] MitoPLD Is a Mitochondrial Protein Essential for Nuage Formation and piRNA Biogenesis in the Mouse Germline (2011)
  • Author(s): Watanabe T, et al
  • Journal Title: Dev Cell Volume: 20 Issue: 3 Pages: 364-375
  • DOI: 10.1016/j.devcel.2011.01.005
  • Peer Reviewed
• [Journal Article] Dynamic stage-specific changes of imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes (2011)
  • Author(s): Tomizawa, S., Kobayashi, H., Watanabe, T., Andrews, S., Hata, K., Kelsey, G. & Sasaki, H
  • Journal Title: Development Volume: 138 Issue: 5 Pages: 811-820
  • DOI: 10.1242/dev.061416
  • Peer Reviewed
• [Journal Article] MitoPLD is a mitochondrialprotein essential for nuage formationand piRNA biogenesis in the mousegermline (2011)
  • Author(s): Watanabe, T, Chuma, S, Yamamoto, Y, Kuramochi-Miyagawa, S, Totoki, Y, Toyoda, A, Hoki, Y, Fujiyama, A, Shibata, T, Sado, T, Noce, T, Nakano, T, Nakatsuji, N, Lin, H, Sasaki, H.
  • Journal Title: Dev Cell 20 Pages: 364-375
  • Peer Reviewed
• [Journal Article] Dynamic stage-specific changes of imprinted differentially methylated regions during early mammalian development and prevalence of non-CpGmethylation in oocytes. (2011)
  • Author(s): Tomizawa, S, Kobayashi, H, Watanabe, T, Andrews, S, Hata, K, Kelsey, G, Sasaki, H.
  • Journal Title: Development 138 Pages: 811-829
  • Peer Reviewed
• [Journal Article] Genomic imprinting in mammals : its life cycle, molecular mechanisms and reprogramming. (2011)
  • Author(s): Li, Y., Sasaki, H.
  • Journal Title: Cell Res Volume: 21 Pages: 466-473
  • NAID: 120004951578
  • Peer Reviewed
• [Journal Article] Dynamic stage-specific changes of imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes. (2011)
  • Author(s): Tomizawa, S., et al
  • Journal Title: Development Volume: 138 Pages: 811-829
  • Peer Reviewed
• [Journal Article] MitoPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline. (2011)
  • Author(s): Watanabe, T., et al
  • Journal Title: Dev.Cell Volume: 20 Pages: 364-375
  • Peer Reviewed
• [Journal Article] Targets and dynamics of promoter DNA methylation during early mouse development (2010)
  • Author(s): Borgel, J., Guibert, S., Li, Y., Chiba, H., Schuebeler, D., Sasaki, H., Forne, T. & Weber, M
  • Journal Title: Nat. Genet Volume: 42 Issue: 12 Pages: 1093-1100
  • DOI: 10.1038/ng.708
  • Peer Reviewed
• [Journal Article] Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development. Nucl (2010)
  • Author(s): Ohnishi, Y., Totoki, Y., Toyoda, A., Watanabe, T., Yamamoto, Y., Tokunaga, K., Sakaki, Y., Sasaki, H. & Hohjoh, H
  • Journal Title: Acids Res Volume: 38 Issue: 15 Pages: 5141-5151
  • DOI: 10.1093/nar/gkq229
  • Peer Reviewed
• [Journal Article] region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip (2010)
  • Author(s): Hiura, H., Sugawara, A., Ogawa, H., John, R., Miyauchi, N., Miyanari, Y., Horiike, T., Li, Y., Yaegashi, N., Sasaki, H., Kono, T. & Arima, T
  • Journal Title: Nucl. Acids Res Volume: 38 Issue: 15 Pages: 4929-4945
  • DOI: 10.1093/nar/gkq200
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa, S., Watanabe, T., Gotoh, K., Takamatsu, K., Chuma, S., Kojima-Kita, K., Shiromoto, Y., Asada, N., Kimura, T., Nakatsuji, N., Noce, T., Sasaki, H. & Nakano, T
  • Journal Title: Genes Dev Volume: 24 Issue: 9 Pages: 887-892
  • DOI: 10.1101/gad.1902110
  • Peer Reviewed
• [Journal Article] Genetic evidence for Dnmt3a-dependent imprinting during oocyte growth obtained by conditional knockout with Zp3-Cre and complete exclusion of Dnmt3b by chimera formation (2010)
  • Author(s): Kaneda, M., Hirawasa, R., Chiba, H., Okano, M., Li, E. & Sasaki, H
  • Journal Title: Genes Cells Volume: 15 Pages: 169-179
  • Peer Reviewed
• [Journal Article] Targets and dynamics of promoter DNA methylation during early mouse development. (2010)
  • Author(s): Borgel, J, Guibert, S, Li, Y, Chiba, H, Schuebeler, D, Sasaki, H, Forne, T, Weber, M.
  • Journal Title: Nat Genet 42 Pages: 1093-1100
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa, S, Watanabe, T, Gotoh, K, Takamatsu, K, Chuma, S, Kojima-Kita, K, Shiromoto, Y, Asada, N, Kimura, T, Nakatsuji, N, Noce, T, Sasaki, H, Nakano, T.
  • Journal Title: Genes Dev 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons. (2010)
  • Author(s): Kuramochi-Miyagawa, S., et al
  • Journal Title: Genes Dev Volume: 24 Pages: 887-892
  • Peer Reviewed
• [Journal Article] A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted do main on mouse chromosome 1 identified by meDIP-on-chip. (2010)
  • Author(s): Hiura, H., et al
  • Journal Title: Nucl.Acids Res Volume: 38 Pages: 4929-4945
  • Peer Reviewed
• [Journal Article] Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development. (2010)
  • Author(s): Ohnishi, Y., et al
  • Journal Title: Nucl.Acids Res Volume: 38 Pages: 5141-5151
  • Peer Reviewed
• [Journal Article] Targets and dynamics of promoter DNA methylation during early mouse development. (2010)
  • Author(s): Borgel, J., et al
  • Journal Title: Nat.Genet Volume: 42 Pages: 1093-1100
  • Peer Reviewed
• [Journal Article] MVH in piRNA processing and gene silencing of retrotransposons (2010)
  • Author(s): Kuramochi-Miyagawa, S.et al
  • Journal Title: Genes Dev. (in press)
  • Peer Reviewed
• [Journal Article] Genetic evidence for Dnmt3a-dependent imprinting during oocyte growth obtained by conditional knockout with Zp3-Cre and complete exclusion of Dnmt3b by chimera formation (2010)
  • Author(s): Kaneda, M.et al
  • Journal Title: Genes Cells (in press)
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji, M., Tanaka, T., Hosokawa, M., Reuter, M., Stark, A., Kato, Y., Kondoh, G., Okawa, K., Chujo, T., Suzuki, T., Hata, K., Martin, S.L., Noce, T., Kuramochi-Miyagawa, S., Nakano, T., Sasaki, H., Pillai, R.S., Nakatsuji, N. & Chuma, S
  • Journal Title: Dev. Cell Volume: 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells (2009)
  • Author(s): Yamaguchi, S., Kurimoto, K., Yabuta, Y., Sasaki, H., Nakatsuji, N., Saitou, M. & Tada, T
  • Journal Title: Development Volume: 136 Issue: 23 Pages: 4011-4020
  • DOI: 10.1242/dev.041160
  • Peer Reviewed
• [Journal Article] Abnormal DNA methyltransferase expression in mouse germline Stem Cells results in spermatogenic defects (2009)
  • Author(s): Takashima, S.,Takehashi, M.,Lee, J., Chuma, S., Okano, M., Hata, K., Suetake, I., Nakatsuji, N.,Miyoshi, H., Tajima, S., Sasaki, H., Kanatsu-Shinohara, M. and Shinohara, T
  • Journal Title: Biol. Reprod Volume: 81 Issue: 1 Pages: 155-164
  • DOI: 10.1095/biolreprod.108.074708
  • Peer Reviewed
• [Journal Article] Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2 (2009)
  • Author(s): Kobayashi H, Yamada K, Morita S, Hiura H, Fukuda A, Kagami M, Ogata T, Hata K, Sotomaru Y, Kono T
  • Journal Title: Genomics Volume: 93(5) Issue: 5 Pages: 461-47
  • DOI: 10.1016/j.ygeno.2008.12.012
  • Peer Reviewed
• [Journal Article] Histone methylation is mechanistically linked to DNA methylation at imprinting control regions in mammals (2009)
  • Author(s): Henckel, A., Nakabayashi, K., Sanz, LA., Feil, R., Hata, K. & Arnaud, P
  • Journal Title: Hum Mol Genet Volume: 18 Issue: 18 Pages: 3375-83
  • DOI: 10.1093/hmg/ddp277
  • Peer Reviewed
• [Journal Article] Dynamic transition of Dnmt3b expression in mouse pre- and early post-implantation embryos (2009)
  • Author(s): Hirasawa, R. & Sasaki, H
  • Journal Title: Gene Expr. Patt Volume: 9 Issue: 1 Pages: 27-30
  • DOI: 10.1016/j.gep.2008.09.002
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mousemale germline (2009)
  • Author(s): Shoji, M, Tanaka, T, Hosokawa, M, Reuter, M, Stark, A, Kato, Y, Kondoh, G, Okawa, K, Chujo, T, Suzuki, T, Hata, K, Martin, SL, Noce, T, Kuramochi-Miyagawa, S, Nakano, T, Sasaki, H, Pillai, RS, Nakatsuji, N, Chuma, S.
  • Journal Title: Dev Cell 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji, M.et al
  • Journal Title: Dev.Cell 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells (2009)
  • Author(s): Yamaguchi, S.et al
  • Journal Title: Development 136 Pages: 4011-4020
  • Peer Reviewed
• [Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects (2009)
  • Author(s): Takashima, S.et al
  • Journal Title: Biol Reprod. 81 Pages: 155-164
  • Peer Reviewed
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline (2009)
  • Author(s): Shoji, M.et al
  • Journal Title: Dev Cell. 17 Pages: 775-787
  • Peer Reviewed
• [Journal Article] Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2 (2009)
  • Author(s): Kobayashi, H.et al
  • Journal Title: Genomics. 93 Pages: 461-472
  • Peer Reviewed
• [Journal Article] Histone methylation is mechanistically linked to DNA methylation at imprinting control regions in mammals (2009)
  • Author(s): Henckel, A.et al
  • Journal Title: Hum Mol Genet. 18 Pages: 3375-3383
  • Peer Reviewed
• [Journal Article] 哺乳類のエピジェネティクス (2009)
  • Author(s): 佐渡敬, ほか
  • Journal Title: 生物の科学:遺伝 63 Pages: 65-70
• [Journal Article] Dynamic transition of Dnmt3b expression in mouse pre-and early post implantation embryos (2009)
  • Author(s): Hirasawa, R., et.al.
  • Journal Title: Gene Expr. Patt. 9 Pages: 27-30
• [Journal Article] Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenesis defects (2009)
  • Author(s): Takashima, S., et.al.
  • Journal Title: Biol. Reprod. (in press)
  • Peer Reviewed
• [Journal Article] De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes (2008)
  • Author(s): Chiba, H., Hirasawa, R., Kaneda, M., Amakawa, Y., Li, E., Sado, T. & Sasaki, H
  • Journal Title: Genesis Volume: 46 Issue: 12 Pages: 768-774
  • DOI: 10.1002/dvg.20496
  • Peer Reviewed
• [Journal Article] Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development (2008)
  • Author(s): Hirasawa, R., Chiba, H., Kaneda, M., Tajima, S., Li, E., Jaenisch, R. & Sasaki, H
  • Journal Title: Genes Dev Volume: 22 Issue: 12 Pages: 1607-1616
  • DOI: 10.1101/gad.1667008
  • Peer Reviewed
• [Journal Article] Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes (2008)
  • Author(s): Watanabe, T., Totoki, Y., Toyoda, A., Kaneda, M., Kuramochi-Miyagawa, S., Obata, Y., Chiba, H., Kohara, Y., Kono, T., Nakano, T., Surani, M.A., Sakaki, Y. & Sasaki, H
  • Journal Title: Nature Volume: 453 Pages: 539-543
  • Peer Reviewed
• [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes (2008)
  • Author(s): Kuramochi-Miyagawa, S., Watanabe, T., Gotoh, K., Totoki, Y., Toyoda, A., Ikawa, M., Asada, N., Kojima, K., Yamaguchi, Y., Ijiri, T., Hata, K., Li, E., Matsuda, Y., Kimura, T., Okabe, M., Sakaki, Y., Sasaki, H. & Nakano, T
  • Journal Title: Genes Dev Volume: 22 Issue: 7 Pages: 908-917
  • DOI: 10.1101/gad.1640708
  • Peer Reviewed
• [Journal Article] Maternal and zygotic Dnmt1 are necessaryand sufficient for the maintenance of DNA methylation imprints during preimplantation development. (2008)
  • Author(s): Hirasawa, R, Chiba, H, Kaneda, M, Tajima, S, Li, E, Jaenisch, R, Sasaki, H.
  • Journal Title: Genes Dev 22 Pages: 1607-1616
  • Peer Reviewed
• [Journal Article] Endogenous siRNAs from naturally for medds RNAs regulate transcripts in mouse oocytes. (2008)
  • Author(s): Watanabe, T, Totoki, Y, Toyoda, A, Kaneda, M, Kuramochi-Miyagawa, S, Obata, Y, Chiba, H, Kohara, Y, Kono, T, Nakano, T, Surani, MA, Sakaki, Y, Sasaki, H.
  • Journal Title: Nature 453 Pages: 539-543
  • Peer Reviewed
• [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. (2008)
  • Author(s): Kuramochi-Miyagawa, S, Watanabe, T, Gotoh, K, Totoki, Y, Toyoda, A, Ikawa, M, Asada, N, Kojima, K, Yamaguchi, Y, Ijiri, T, Hata, K, Li, E, Matsuda, Y, Kimura, T, Okabe, M, Sakaki, Y, Sasaki, H, Nakano, T.
  • Journal Title: Genes Dev 22 Pages: 908-917
  • Peer Reviewed
• [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes (2008)
  • Author(s): Kuramochi-Miyagawa, S., et.al.
  • Journal Title: Genes Dev. 22 Pages: 908-917
  • Peer Reviewed
• [Journal Article] Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes (2008)
  • Author(s): Watanabe, T., et.al.
  • Journal Title: Nature 453 Pages: 539-543
  • Peer Reviewed
• [Journal Article] Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development (2008)
  • Author(s): Hirasawa, R., et.al.
  • Journal Title: Genes Dev. 22 Pages: 1607-1616
  • Peer Reviewed
• [Journal Article] De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes (2008)
  • Author(s): Chiba, H., et.al.
  • Journal Title: Genesis 46 Pages: 768-774
  • Peer Reviewed
• [Journal Article] ゲノムインプリンティング (2008)
  • Author(s): 平澤竜太郎ほか
  • Journal Title: 生体の科学(増大特集)現代医学・生物学の仮説・学説2008 59 Pages: 402-403
• [Journal Article] カレントトピックス : マウス卵子において内在性siRNAは自然に形成される二本鎖RNAから作られ、転写物を制御している (2008)
  • Author(s): 渡部聡朗
  • Journal Title: 実験医学 26 Pages: 2109-2111
• [Journal Article] Role for piRNAs and non-coding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus.
  • Author(s): Watanabe, T, Tomizawa, S, Mitsuya, K, Totoki, Y, Yamamoto, Y, Kuramochi-Miyagawa, S, Iida, N, Hoki, Y, Murphy, PJ, Toyoda, A, Gotoh, K, Hiura, H, Arima, T, Fujiyama, A, Sado, T, Shibata, T, Nakano, T, Lin, H, Ichiyanagi, K, Soloway, PD, Sasaki, H.
  • Journal Title: Science in press
  • Peer Reviewed
• [Presentation] 次世代遺伝子解析技術を用いた生殖発生異常のゲノム研究 (2012)
  • Author(s): 秦健一郎
  • Organizer: 第30回日本受精着床学会学術講演会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)(招待講演)
  • Year and Date: 2012-08-31
• [Presentation] The regulatory network of small RNAs and DNA methylation in mouse germ cells revealed by high-throughput sequencing combined with genetic studies (2012)
  • Author(s): Sasaki, H.
  • Organizer: Computational Biology Research Center Workshop 2011, AIST
  • Place of Presentation: 産業技術総合研究所(東京)(招待講演)
  • Year and Date: 2012-01-26
• [Presentation] DNAメチル化と小分子RNAの網羅的解析がもたらす新発見 (2011)
  • Author(s): 佐々木裕之
  • Organizer: 第34回日本分子生物学会年会バイオテクノロジーセミナー
  • Place of Presentation: パシフィコ横浜(神奈川)(招待講演)
  • Year and Date: 2011-12-16
• [Presentation] DNAメチル化に依らないゲノムインプリンティング (2011)
  • Author(s): 千葉初音, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-16
• [Presentation] Role of small RNAs in shaping the epigenome of germ cells for genome defense and imprinting (2011)
  • Author(s): Sasaki, H.
  • Organizer: 第27回国際生物学賞記念シンポジウム
  • Place of Presentation: 京都ガーデンパレス(京都)(招待講演)
  • Year and Date: 2011-11-30
• [Presentation] The regulatory network of small RNAs and DNA methylation in mouse germ cells (2011)
  • Author(s): Sasaki, H.
  • Organizer: EpiGeneSys 1st Annual Meeting, IMBA
  • Place of Presentation: Institute fur Molekulare Biotechnologie GmbH, Vienna, Austria(招待講演)
  • Year and Date: 2011-11-25
• [Presentation] Germline small RNA and imprinting (2011)
  • Author(s): Sasaki, H.
  • Organizer: ICC on Genomic Imprinting and Beyond 2011
  • Place of Presentation: Institut d'Estudis Catalans, Barcelona, Spain(招待講演)
  • Year and Date: 2011-09-23
• [Presentation] Role of small RNAs in shaping the epigenome of germ cells for genome defense and imprinting (2011)
  • Author(s): Sasaki, H.
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本大学工学部百周年記念館(熊本)(招待講演)
  • Year and Date: 2011-09-09
• [Presentation] 哺乳類生殖細胞の小分子RNAとエピゲノム制御Small RNA and epigenomic regulation in mammalian germ cells (2011)
  • Author(s): 佐々木裕之
  • Organizer: 第3回日本RNAi研究会
  • Place of Presentation: グランドプリンスホテル広島(広島)(招待講演)
  • Year and Date: 2011-08-27
• [Presentation] Mammalian germline small RNAs and epigenetic programming. Gordon Research Conference (Epigenetics) (2011)
  • Author(s): Sasaki, H
  • Organizer: Easton
  • Place of Presentation: MA, USA
  • Year and Date: 2011-08-09
• [Presentation] Mammalian germline small RNAs and epigenetic programming (2011)
  • Author(s): Sasaki, H.
  • Organizer: Gordon Research Conferences (Epigenetics)
  • Place of Presentation: Stonehill College, Easton, MA (USA)(招待講演)
  • Year and Date: 2011-08-09
• [Presentation] Role of epigenetics and small RNAs in genome defense and developmental control in mammalian germ cell (2011)
  • Author(s): Sasaki, H.
  • Organizer: The 6^<th> International Symposium of InstituteNetwork and the 10^<th> Surugadai International Symposium, Joint Usage/Research Program of Medical Research Institute International Symposium
  • Place of Presentation: 東京医科歯科大学鈴木章夫記念講堂(東京)(招待講演)
  • Year and Date: 2011-06-10
• [Presentation] Role of Small RNAs in Shaping the Epigenome of Germ Cells for Genome Defense and Imprinting (2011)
  • Author(s): Sasaki, H.
  • Organizer: 6th Asian Epigenomics Meeting
  • Place of Presentation: KKRホテル熊本(熊本)(招待講演)
  • Year and Date: 2011-05-18
• [Presentation] 生殖細胞のエピゲノムと小分子RNA (2010)
  • Author(s): 佐々木裕之
  • Organizer: 第4回 がん・エピゲノム研究会
  • Place of Presentation: 仙台
  • Year and Date: 2010-12-15
• [Presentation] 生殖細胞のエピゲノムと小分子RNA (2010)
  • Author(s): 佐々木裕之
  • Organizer: エピゲノム研究の最先端
  • Place of Presentation: 東京
  • Year and Date: 2010-11-02
• [Presentation] Role for Piwi-interacting RNA and a novel noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus in the germline. (2010)
  • Author(s): Sasaki, H., Watanabe, T.
  • Organizer: Cold Spring Harbor Asia Meeting on Epigenetics, Chromatin & Transcription.
  • Place of Presentation: Suzhou, China.
• [Presentation] Role for Piwi-interacting RNA and a novel noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus in the germline (2010)
  • Author(s): Sasaki, H., Watanabe, T.
  • Organizer: Epigenetics, Chromatin & Transcription
  • Place of Presentation: Suzhou, China
• [Presentation] Role for Piwi-interacting RNA and a novel noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus in the germline (2010)
  • Author(s): Sasaki, H.
  • Organizer: 第43回 日本発生生物学会年会
  • Place of Presentation: 京都
• [Presentation] Epigenetics and small RNA systems in mammalian male germ cells (2010)
  • Author(s): Sasaki, H.
  • Organizer: 11th International Symposium on Spermatology
  • Place of Presentation: Okinawa
• [Presentation] Epigenetics in human disorder (2010)
  • Author(s): 佐々木裕之
  • Organizer: 第16回 日本遺伝子治療学会学術集会
  • Place of Presentation: 宇都宮
• [Presentation] 哺乳類生殖細胞のエピジェネティクスと小分子RNA (2010)
  • Author(s): 佐々木裕之
  • Organizer: 第6回 日本先天代謝異常学会セミナー
  • Place of Presentation: 横浜
• [Presentation] Small RNAs, retrotransposons and genomic imprinting in the mammalian germline (2010)
  • Author(s): Sasaki, H., Watanabe, T.
  • Organizer: The 20th hot Spring Harbor Symposium and joint with the 6th Global COE International Symposium
  • Place of Presentation: Fukuoka
• [Presentation] 哺乳類生殖細胞のエピジェネティクスと小分子RNA (2010)
  • Author(s): 佐々木裕之
  • Organizer: 第5回 認識と形成研究会
  • Place of Presentation: 阿蘇
• [Presentation] 哺乳類生殖細胞におけるゲノムインプリンティング制御 (2010)
  • Author(s): 佐々木裕之
  • Organizer: 日本遺伝学会 第82回大会
  • Place of Presentation: 札幌
• [Presentation] Genomic imprinting and small RNAs in mammalian germ cells (2010)
  • Author(s): Sasaki, H.
  • Organizer: The 16th International Conference of the International Society of Differentiation
  • Place of Presentation: Nara
• [Presentation] Genomic imprinting, DNA methylation and small RNA in male germ cell development (2010)
  • Author(s): Sasaki, H., Watanabe, T.
  • Organizer: International Symposium on "Epigenome Network, Development and Reprogramming of Germ Cells
  • Place of Presentation: Fukuoka
• [Presentation] Genomic imprinting and small RNAs in the mammalian germ cells (2010)
  • Author(s): Sasaki, H.
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] The epigenome and functional small RNAs (2010)
  • Author(s): 佐々木裕之
  • Organizer: Korea-Japan Chromatin & Epigenetics Joint Symposlum 2010
  • Place of Presentation: Hoengseong Gun, Korea
• [Presentation] 哺乳類生殖細胞のエピジェネティクスと小分子RNA (2009)
  • Author(s): 佐々木裕之
  • Organizer: 第7回RCGMフロンティアシンポジウム"未来医療のためのゲノム医学研究"
  • Place of Presentation: 埼玉
  • Year and Date: 2009-11-03
• [Presentation] Roles of genomic imprinting in reproduction (2009)
  • Author(s): 秦健一郎
  • Organizer: The 7th Conference of the Pacific Rim Society for Fertility and Sterility
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2009-08-12
• [Presentation] 胎児・胎盤分化発育異常のエピジェネティクス-網羅的ゲノム・エピゲノム解析の試み- (2009)
  • Author(s): 秦健一郎
  • Organizer: 日本周産期新生児学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-07-12
• [Presentation] エピジェネティクスが拓く生命科学 (2009)
  • Author(s): 佐々木裕之
  • Organizer: 2009 Millipore Asia BioForum 幹細胞およびエピジェネティクス研究の最前線
  • Place of Presentation: 東京
  • Year and Date: 2009-06-15
• [Presentation] Epigenetics and small RNAs in mammalian germ cells (2009)
  • Author(s): 佐々木裕之
  • Organizer: TriRhena Chromatin/Transcription Club Meeting, Freiburg
  • Place of Presentation: Freiburg, Germany
  • Year and Date: 2009-05-12
• [Presentation] Genomic imprinting, DNA methylation and small RNAs in mammalian germ cells (2009)
  • Author(s): Sasaki, H.
  • Organizer: Gordon Research Conference "Epigenetics- The Role of the Environment and Epigenetic Mechanisms in Bahavior, Health, and Disease", Holderness
  • Place of Presentation: NH, USA
• [Presentation] Imprinting, DNA methylation and small RNA in germ cells (2009)
  • Author(s): Sasaki, H
  • Organizer: Cold Spring Harbor Laboratory/Wellcome Trust Joint Conference "Mouse Genetics & Genomics: Development & Disease"
  • Place of Presentation: Hinxton, UK
• [Presentation] Genomic imprinting, DNA methylation and small RNAs in mammaliangerm cells. (2009)
  • Author(s): Sasaki, H.
  • Organizer: Gordon Research Conferenceon Epigenetics-The Role of the Environment and Epigenetic Mechanisms
  • Place of Presentation: Holderness, NH, USA.
• [Presentation] The epigenome and small RNA repertoire in mammalian germ cells. (2009)
  • Author(s): Sasaki, H.
  • Organizer: EMBO Conference Series on Chromatin and Epigenetics
  • Place of Presentation: Heidelberg, Germany.
• [Presentation] The epigenome and small RNA repertoire in mammalian germ cells (2009)
  • Author(s): 佐々木裕之
  • Organizer: EMBO Conference Series on Chromatin and Epigenetics
  • Place of Presentation: Heidelberg, Germany
• [Presentation] The epigenome and small RNA repertoire in mammalian germ cells (2009)
  • Author(s): 佐々木裕之
  • Organizer: The 24th Naito Conference on Nuclear Dynamics and RNA[II]
  • Place of Presentation: 札幌
• [Presentation] 発生・成長におけるエピジェネティクス (2009)
  • Author(s): 佐々木裕之
  • Organizer: 第13回小児分子内分泌研究会
  • Place of Presentation: 函館
• [Presentation] 生殖細胞のエピゲノムと機能性RNA (2009)
  • Author(s): 佐々木裕之
  • Organizer: 第33回阿蘇シンポジウム生命科学のフロントランナー
  • Place of Presentation: 熊本
• [Presentation] Genomic imprinting, DNA methylation and small RNAs in mammalian germ cells (2009)
  • Author(s): 佐々木裕之
  • Organizer: Gordon Research Conference "Epigenetics-The Role of the Environment and Epigenetic Mechanisms in Behavior, Health, and Disease"
  • Place of Presentation: Holderness, NH, USA
• [Presentation] Imprinting, DNA methylation and small RNA in germ cells (2009)
  • Author(s): 佐々木裕之
  • Organizer: Cold Spring Harbor Laboratory/Wellcome Trust Joint Conference "Mouse Genetics & Genomics : Development & Disease"
  • Place of Presentation: Hinxton, UK.
• [Presentation] 次世代シークエンサーによる機能性小分子RNAの網羅的解析 (2009)
  • Author(s): 佐々木裕之
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 松本
• [Presentation] ゲノムインプリンティングのエピゲノム機構に関する研究 (2009)
  • Author(s): 佐々木裕之
  • Organizer: 日本人類遺伝学会第54回大会
  • Place of Presentation: 東京
• [Presentation] シンポジウム "Emerging power of epigenomics in life science" (2009)
  • Author(s): 佐々木裕之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Endogenous siRNAs derived from naturally formed dsRNAs regulate transcripts in mouse oocytes (2008)
  • Author(s): 佐々木裕之ほか
  • Organizer: 第60回日本細胞生物学会大会シンポジウム「分化とepigenetic制御」
  • Place of Presentation: 横浜
• [Presentation] Endogenous siRNAs derived from naturally formed dsRNAs regulate coding-transcripts and retrotransposons in mouse oocytes (2008)
  • Author(s): Sasaki, H.
  • Organizer: The 2nd Shanghai Symposium on Epigenetics in Development and Diseases/the 3rd Annual Meeting of Asian Epigenome Alliance
  • Place of Presentation: Shanghai
• [Presentation] Endogenous siRNAs derived from naturally formed dsRNAs regulate coding-transcripts and retrotransposons in mouse oocytes (2008)
  • Author(s): Sasaki, H.
  • Organizer: International Conference on Epigenetics 2008
  • Place of Presentation: Berlin
• [Presentation] 内在性siRNAはマウスの卵子において自然に形成される二本鎖RNAから作られ、遺伝子やレトロトランスポゾンを制御する (2008)
  • Author(s): 佐々木裕之ほか
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋
• [Presentation] Gametogenesis and imprinting in mice (2008)
  • Author(s): Sasaki, H.
  • Organizer: EMBO Workshop on Genomic Imprinting
  • Place of Presentation: Singapore
• [Presentation] 生殖細胞の発生・リプログラミングとエピジェネティクス (2008)
  • Author(s): 佐々木裕之
  • Organizer: 日本人類遺伝学会第53回大会
  • Place of Presentation: 横浜(シンポジウム)
• [Presentation] 哺乳類の生殖細胞系列におけるゲノムインプリンティングのプログラム機構 (2008)
  • Author(s): 佐々太裕之
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸(シンポジウム)
• [Presentation] The epigenome and small RNA repertoire in mammalian germ cells (2008)
  • Author(s): Sasaki, H.
  • Organizer: International Symposium Decoding Epigenetic Code
  • Place of Presentation: Tokyo
• [Book] 卵子学 (2011)
  • Author(s): 佐々木裕之
  • Publisher: 京都大学学術出版会
• [Book] 4章.ゲノムの高度活用戦術-エピジェネティクス.現代生物科学入門1ゲノム科学の基礎 (2009)
  • Author(s): 佐々木裕之
  • Publisher: 岩波書店
• [Book] Genomic imprinting and X chromosome inactivation in germ cell development. Genetic and Epinenetic Control of Mammalian Germ Cell Development and Function (ed. Nozaki, M) (2009)
  • Author(s): Kaneda, M. & Sasaki, H.
  • Publisher: Research Signopost
• [Book] 現代生物科学入門-1ゲノム科学の基礎(浅島誠, 黒岩常祥, 小原雄治編)(4章ゲノムの高度活用戦略-エピジェネティクス) (2009)
  • Author(s): 佐々木裕之
  • Publisher: 岩波書店
• [Book] 4章.ゲノムの高度活用戦略-エピジェネティクス. 現代生物科学入門1ゲノム科学の基礎(浅島誠, 黒岩常祥, 小原雄治編) (2009)
  • Author(s): 佐々木裕之
  • Publisher: 岩波書店
• [Book] Genomic imprinting and X chromosome inactivation in germ cell development. Genetic and Epigenetic Control of Mammalian Germ Cell Development and Function(ed.Nozaki, M.) (2009)
  • Author(s): Kaneda, M.et al
  • Publisher: Research Signpost
• [Book] 生殖細胞分化とエピジェネティクス.転写制御の分子生物学-ゲノムデコードに向けて(加藤茂明編) (2008)
  • Author(s): 平澤竜太郎ほか
  • Publisher: 南山堂
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/epigenome/index.html
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html#
• [Remarks] 研究室ホームページ
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/epigenome/
• [Remarks] 研究室ホームページ
  • URL: http://www.bioreg.kyushu-u.ac.jp/labo/epigenome/
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/HumGen/index.html
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/HumGen/index.html