The mechanism of the acquisition of germ cell-specific epigenome and its reconstruction in vitro.

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062011
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University (2009-2012); The Institute of Physical and Chemical Research (2008)
• Principal Investigator: SAITOU Mitinori 京都大学, 医学研究科, 教授 (80373306)
• Co-Investigator(Kenkyū-buntansha): OHINATA Yasuhide (70415107) ; UEDA Jun (80450394)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥63,500,000 (Direct Cost: ¥63,500,000); Fiscal Year 2012: ¥12,700,000 (Direct Cost: ¥12,700,000); Fiscal Year 2011: ¥12,700,000 (Direct Cost: ¥12,700,000); Fiscal Year 2010: ¥12,700,000 (Direct Cost: ¥12,700,000); Fiscal Year 2009: ¥12,700,000 (Direct Cost: ¥12,700,000); Fiscal Year 2008: ¥12,700,000 (Direct Cost: ¥12,700,000)
• Keywords: 発生・分化 / 遺伝子 / ゲノム / シグナル伝達 / 再生医学 / 生殖細胞 / Blimpl / Prdm14 / エピゲノム / Bmp4 / エピブラスト / 細胞培養 / ES細胞

Research Abstract

We clarified the mechanism underlying the specification of primordial germ cells (PGCs), the precursors both for spermatozoa and oocytes, in mice. Based on the knowledge obtained, we succeeded in reconstituting mouse germ cell specification pathway in culture by pluripotent stem cells including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). The induced PGC-like cells contributed to spermatozoa and oocytes, and to fertile offspring. We also clarified the role of PRDM14 in the maintenance of pluripotency in mouse ESCs.

Research Products

• [Journal Article] PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in mouse embryonic stem cells (2013)
  • Author(s): Yamaji, M., Ueda, J., Hayashi, K., Ohta, H., Yabuta, Y., Kurimoto, K., Nakato, R., Shirahige, K., and Saitou, M
  • Journal Title: Cell Stem Cell Volume: 12 Issue: 3 Pages: 368-382
  • DOI: 10.1016/j.stem.2012.12.012
  • Peer Reviewed
• [Journal Article] Replication-coupled passive DNA demethylation for the erasure of genome imprints in mice (2013)
  • Author(s): Kagiwada, S., Kurimoto, K., Hirota, T., Yamaji, M., and Saitou, M
  • Journal Title: The EMBO Journal Volume: 32 Issue: 3 Pages: 340-353
  • DOI: 10.1038/emboj.2012.331
  • Peer Reviewed
• [Journal Article] Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice (2012)
  • Author(s): Hayashi, K., Ogushi, S., Kurimoto, K., Shimamoto, S., Ohta, H., Saitou, M
  • Journal Title: Science Volume: 338 Issue: 6109 Pages: 971-975
  • DOI: 10.1126/science.1226889
  • Peer Reviewed
• [Journal Article] Non conservation of function for the evolutionarily conserved Prdml protein in the control of the slow twitch myogenic program in the mouse embryo (2012)
  • Author(s): Vincent, S. D., Mayeuf, A., Niro, C., Saitou, M., Buckingham, M.
  • Journal Title: Molecular Biology and Evolution Volume: 29 Pages: 3181-3191
  • Peer Reviewed
• [Journal Article] Primordial germ cells in mice (2012)
  • Author(s): Saitou, M., Yamaji, M.
  • Journal Title: CSH Perspectives in Biology Volume: 4 Issue: 11 Pages: a008375-a008375
  • DOI: 10.1101/cshperspect.a008375
  • Peer Reviewed
• [Journal Article] Germline development from human pluripotent stem cells toward disease modeling of infertility (2012)
  • Author(s): Hayashi, Y., Saitou, M., Yamanaka, S.
  • Journal Title: ertility and Sterility Volume: 97 Issue: 6 Pages: 1250-1259
  • DOI: 10.1016/j.fertnstert.2012.04.037
  • Peer Reviewed
• [Journal Article] Primordial germ cells in mice (2012)
  • Author(s): Saitou, M., Yamaji, M.
  • Journal Title: CSH Perspectives Volume: (In press)
  • Peer Reviewed
• [Journal Article] Epigenetic reprogramming in mouse pre-implantation development and primordial germ cells (2012)
  • Author(s): Saitou, M., Kagiwada, S., Kurimoto, K.
  • Journal Title: Development Volume: 139 Issue: 1 Pages: 15-31
  • DOI: 10.1242/dev.050849
  • Peer Reviewed
• [Journal Article] Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells (2011)
  • Author(s): 林克彦, 栗本一基, 大田浩, 荒牧伸弥, 斎藤通紀
  • Journal Title: Cell Volume: 146 Issue: 4 Pages: 519-532
  • DOI: 10.1016/j.cell.2011.06.052
  • Peer Reviewed
• [Journal Article] Drug-inducible gene recombination by the Dppa3-MER Cre MER transgene in the developmental cycle of the germ cell lineage in mice (2011)
  • Author(s): Hirota, T., Ohta, H., Shigeta, M., Niwa, H., and Saitou, M
  • Journal Title: Biology of Reproduction Volume: 85 Issue: 2 Pages: 367-377
  • DOI: 10.1095/biolreprod.110.090662
  • Peer Reviewed
• [Journal Article] TDRD5 is required for retrotransposon silencing, chromatoid body assembly and spermiogenesis in mice (2011)
  • Author(s): Yabuta, Y., Ohta, H., Abe, T., Kurimoro, K., Chuma, S., and Saitou, M
  • Journal Title: The Journal of Cell Biology Volume: 192 Issue: 5 Pages: 781-795
  • DOI: 10.1083/jcb.201009043
  • Peer Reviewed
• [Journal Article] Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development (2011)
  • Author(s): Ichiyanagi, T., Fukuda, K., Kitayama, J., Yamamoto, Y., Kuramochi-Miyagawa, S., Nakano, T., Yabuta, Y., Seki, Y., Saitou, M., & Sasaki, H
  • Journal Title: Genome Res Volume: 21 Issue: 12 Pages: 2058-2066
  • DOI: 10.1101/gr.123679.111
  • Peer Reviewed
• [Journal Article] Loss of hererochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell-cycle progression in mice (2011)
  • Author(s): Abe, K., et al
  • Journal Title: Biology of Reproduction Volume: 85 Issue: 5 Pages: 1013-1024
  • DOI: 10.1095/biolreprod.111.091512
  • Peer Reviewed
• [Journal Article] Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes (2011)
  • Author(s): J.Lee
  • Journal Title: Mol.Biol.Cell Volume: 22 Issue: 18 Pages: 3465-3477
  • DOI: 10.1091/mbc.e11-05-0423
  • Peer Reviewed
• [Journal Article] TDRD5 is required for retrotransposon silencing, chromatoid body assembly and spermiogenesis in mice. (2011)
  • Author(s): Yabuta, Y., Ohta, H., Abe, T., Kurimoro, K., Chuma, S., Saitou, M.
  • Journal Title: The Journal of Cell Biology 192 Pages: 781-795
  • Peer Reviewed
• [Journal Article] Involvement of murine Nucleoplasmin 2 in the decondensation of sperm chromatin after fertilization. (2011)
  • Author(s): Inoue, A., Ogushi, S., Saitou, M., Suzuki, M.G., Aoki, F.
  • Journal Title: Biology of Reproduction Volume: (In press)
  • Peer Reviewed
• [Journal Article] TDRD5 is required for retrotransposon silencing, chromatoid body assembly and spermiogenesis in mice. (2011)
  • Author(s): Yabuta, Y., Ohta, H., Abe, T., Kurimoro, K., Chuma, S., Saitou, M.
  • Journal Title: The Journal of Cell Biology Volume: 192 Pages: 781-795
  • Peer Reviewed
• [Journal Article] A global single-cell cDNA amplification method for quantitative microarray analysis. (2011)
  • Author(s): Kurimoto, K., Saitou, M.
  • Journal Title: Methods in Molecular Biology Volume: 687 Pages: 91-111
• [Journal Article] Functional reconstruction of Nanos3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of Nanos3 (2010)
  • Author(s): Yamaji, M., Tanaka, T., Shigeta, M., Chuma, S., Saga, Y., and Saitou, M
  • Journal Title: Reproduction Volume: 139 Issue: 2 Pages: 381-393
  • DOI: 10.1530/rep-09-0373
• [Journal Article] Functional reconstruction of Nanos3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of Nanos3. (2010)
  • Author(s): Yamaji, M., Tanaka, T., Shigeta, M., Chuma, S., Saga, Y., Saitou, M.
  • Journal Title: Reproduction 139 Pages: 381-393
  • Peer Reviewed
• [Journal Article] Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system. (2010)
  • Author(s): Yamagata, K., Ueda, J., Mizutani, E., Saitou, M., Wakayama, T.
  • Journal Title: Developmental Biology Volume: 346 Pages: 90-101
  • Peer Reviewed
• [Journal Article] Single-cell cDNA microarray profiling of complex biological processes of differentiation. (2010)
  • Author(s): Kurimoto, K., Saitou, M.
  • Journal Title: Current Opinion in Genetics and Development Volume: 20 Pages: 470-477
  • NAID: 120002647326
  • Peer Reviewed
• [Journal Article] The nucleolus in the mouse oocyte is required for the early step of both female and male pronucleus organization. (2010)
  • Author(s): Ogushi, S., Saitou, M.
  • Journal Title: Journal of Reproduction and Development Volume: 56 Pages: 495-501
  • NAID: 10027571739
  • Peer Reviewed
• [Journal Article] Germ cell specification in mice : Signalling, transcription regulation and epigenetic consequences. (2010)
  • Author(s): Saitou, M., Yamaji, M.
  • Journal Title: Reproduction Volume: 139 Pages: 931-942
  • Peer Reviewed
• [Journal Article] A signaling principle for the specification of the germ cell lineage in mice (2009)
  • Author(s): Ohinata, Y., Ohta, H., Shigeta, M., Yamanaka, K., Wakayama, T., and Saitou, M
  • Journal Title: Cell Volume: 137 Issue: 3 Pages: 571-584
  • DOI: 10.1016/j.cell.2009.03.014
• [Journal Article] A signaling principle for the specification of the germ cell lineage in mice. (2009)
  • Author(s): Ohinata, Y., Ohta, H., Shigeta, M., Yamanaka, K., Wakayama, T., Saitou, M.
  • Journal Title: Cell 137 Pages: 571-584
  • Peer Reviewed
• [Journal Article] Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells. (2009)
  • Author(s): Yamaguchi, S., Kurimoto, K., Yabuta, Y., Sasaki, H., Nakatsuji, N., Saitou, M., Tada, T.
  • Journal Title: Development 136 Pages: 4011-4020
  • Peer Reviewed
• [Journal Article] Germ cell specification in mice. (2009)
  • Author(s): Saitou, M.
  • Journal Title: Current Opinion in Genetics and Development 19 Pages: 386-395
  • Peer Reviewed
• [Journal Article] Specification of the germ cell lineage in mice (2009)
  • Author(s): Saitou, M.
  • Journal Title: Frontiers in Bioscience 14 Pages: 1068-1087
  • Peer Reviewed
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice (2008)
  • Author(s): Yamaji, M., Seki, Y., Kurimoto, K., Yabuta, Y., Yuasa, M., Shigeta, M., Yamanaka, K., Ohinata, Y., and Saitou, M
  • Journal Title: Nature Genetics Volume: 40 Issue: 8 Pages: 1016-1022
  • DOI: 10.1038/ng.186
• [Journal Article] Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice (2008)
  • Author(s): Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K., and Saitou, M
  • Journal Title: Genes & Development Volume: 22 Issue: 12 Pages: 1617-1635
  • DOI: 10.1101/gad.1649908
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice. (2008)
  • Author(s): Yamaji, M., Seki, Y., Kurimoto, K., Yabuta, Y., Yuasa, M., Shigeta, M., Yamanaka, K., Ohinata, Y., Saitou, M.
  • Journal Title: Nature Genetics 40 Pages: 1016-1022
  • Peer Reviewed
• [Journal Article] Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice. (2008)
  • Author(s): Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K., Saitou, M.
  • Journal Title: Genes & Development 22 Pages: 1617-1635
  • Peer Reviewed
• [Journal Article] Specification of the germ cell lineage in mice : A Process orchestratedby the PR-domain proteins, Blimpl and Prdm14 (2008)
  • Author(s): Kurimoto, K., et al.
  • Journal Title: Cell Cycle 7 Pages: 3514-3518
• [Journal Article] Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis (2008)
  • Author(s): Kawaguchi, A., et al.
  • Journal Title: Development 135 Pages: 3113-3124
  • Peer Reviewed
• [Journal Article] A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Blinmpl-mVenus and stella-ECFP double transgenic reporter (2008)
  • Author(s): Ohinata, Y., et al.
  • Journal Title: Reproduction 136 Pages: 503-514
  • Peer Reviewed
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice (2008)
  • Author(s): Yamaji, M., et al.
  • Journal Title: Neture Genetics 40 Pages: 1016-1022
  • Peer Reviewed
• [Journal Article] Complex genome-wide transcription dynamics orchestrated by Blimpl for the specification of the germ cell lineage in mice (2008)
  • Author(s): Kurimoto, K., et al.
  • Journal Title: Genes&Development 22 Pages: 1617-1635
  • Peer Reviewed
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2013)
  • Author(s): 斎藤通紀
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2013-03-22
• [Presentation] Mechanism and reconstitution in vitro of germ cell development in mice (2013)
  • Author(s): 斎藤通紀
  • Organizer: The Croucher Foundation Advanced Study Institute on Stem Cells : Niches, Regeneration & Repair
  • Place of Presentation: The University of Hong Kong (香港)
  • Year and Date: 2013-03-05
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2013)
  • Author(s): 斎藤通紀
  • Organizer: 第9回北大若手研究者の会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2013-02-20
• [Presentation] iPS細胞は医療を変えるか? (2013)
  • Author(s): 斎藤通紀
  • Organizer: 朝日 健康・医療フォーラム2013
  • Place of Presentation: TFTホール東京ファッションダウンヒル(東京都)
  • Year and Date: 2013-02-11
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-12
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): Saitou, M.
  • Organizer: EMBO/EMBL "From Functional Genomics to Systems Biology" meeting
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2012-11-18
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: EMBO Conference Series : From Functional Genomics to Systems Biology
  • Place of Presentation: EMB Advanced Training Center (ドイツ)
  • Year and Date: 2012-11-18
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: The Sixth International Conference on Cell Therapy
  • Place of Presentation: Seoul National University Hospital (韓国)
  • Year and Date: 2012-10-26
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): Saitou, M
  • Organizer: EMBO/EMBL Symposium
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2012-10-16
• [Presentation] Mechanism and reconstituttion in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: EMBO/EMBL Symposium : Germline-Immortality through Topipotency
  • Place of Presentation: EMBL Advanced Training Center (ドイツ)
  • Year and Date: 2012-10-16
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): Saitou, M
  • Organizer: The 4th EMBO meeting
  • Place of Presentation: Nice, France
  • Year and Date: 2012-09-25
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: the 4th EMBO meeting
  • Place of Presentation: Acropolis Convention Centre (フランス)
  • Year and Date: 2012-09-25
• [Presentation] 多能性幹細胞からの生殖細胞作成研究の現状と展望 (2012)
  • Author(s): 斎藤通紀
  • Organizer: 日本人類遺伝学会 第22回遺伝医学セミナー
  • Place of Presentation: ホアル阪急エキスポパーク
  • Year and Date: 2012-09-08
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2012)
  • Author(s): 斎藤通紀
  • Organizer: BD学術セミナー2012
  • Place of Presentation: ザ・リッツ・カールトン大阪(大阪府)
  • Year and Date: 2012-09-07
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: ASPIRE 2012
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2012-09-02
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: The 58th/60th NIBB Conference : Germline
  • Place of Presentation: 岡崎コンフェランスセンター(愛知県)
  • Year and Date: 2012-07-18
• [Presentation] 多能性幹細胞による生殖細胞発生過程の試験管内再構成:現状と展望 (2012)
  • Author(s): 斎藤通紀
  • Organizer: 日本アンドロロジー学会第31回学術大会
  • Place of Presentation: 神戸ポートピアホテル(兵庫県)
  • Year and Date: 2012-06-29
• [Presentation] Towards in vitro reconstitution of mammalian germ cell development (2012)
  • Author(s): Saitou, M
  • Organizer: ISSCR 10^<th> Annual Meeting
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-14
• [Presentation] Towards in vitro reconstitution of mammalian germ cell development (2012)
  • Author(s): 斎藤通紀
  • Organizer: ISSCR 10th Annual Meeting
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2012-06-14
• [Presentation] Germ cell specification in mice in vivo and in vitro (2012)
  • Author(s): 斎藤通紀
  • Organizer: The Second SKLRB Symposium : Frontiers in Reproductive Biology
  • Place of Presentation: Xijiao Hotel (中国)
  • Year and Date: 2012-05-07
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): 斎藤通紀
  • Organizer: 2012 CSH Asia Conference : Epigenetics, Chromatin & Transcription
  • Place of Presentation: Suzhou Dushu Lake Conference Center (中国)
  • Year and Date: 2012-04-25
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2012)
  • Author(s): 斎藤通紀
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2012-04-22
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2012)
  • Author(s): 斎藤通紀
  • Organizer: 日本生殖再生医学会第7回学術集会
  • Place of Presentation: シェーンバッハ・サボー(砂防会館別館会議室)(招待講演)
  • Year and Date: 2012-03-25
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2012)
  • Author(s): 斎藤通紀
  • Organizer: 東京大学大学院医学系研究科疾患生命工学センターシンポジウム
  • Place of Presentation: 東京大学医学部教育研究棟14階鉄門記念講堂(招待講演)
  • Year and Date: 2012-03-08
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2012)
  • Author(s): 斎藤通紀
  • Organizer: 第2回産婦人科スプリングフォーラム
  • Place of Presentation: 京都平安ホテル(招待講演)
  • Year and Date: 2012-03-03
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2012)
  • Author(s): Mitinori Saitou
  • Organizer: CiRA International Symposium 2012
  • Place of Presentation: Clock Tower Centennial Hall, Kyoto University(招待講演)
  • Year and Date: 2012-02-23
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2012)
  • Author(s): 斎藤通紀
  • Organizer: 第36回阿蘇シンポジウム
  • Place of Presentation: 阿蘇リソートグランヴィリオホテル(熊本県)
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2011)
  • Author(s): Mitinori Saitou
  • Organizer: 27th RBC-NIRS International Symposium
  • Place of Presentation: コープ・イン・京都(招待講演)
  • Year and Date: 2011-12-10
• [Presentation] 生殖細胞の発生機構とその試験管内再構成 (2011)
  • Author(s): 斎藤通紀
  • Organizer: 第26回日本生殖免疫学会総会・学術集会
  • Place of Presentation: ウィンクあいち(招待講演)
  • Year and Date: 2011-12-03
• [Presentation] マウスES細胞の未分化性維持におけるPrdm14の役割 (2011)
  • Author(s): 斎藤通紀
  • Organizer: 生殖系列の世代サイクルとエピゲノムネットワーク第4回公男シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(招待講演)
  • Year and Date: 2011-11-17
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2011)
  • Author(s): 斎藤通紀
  • Organizer: 発生工学・疾患モデル研究会第89回定例会
  • Place of Presentation: 順天堂大学医学部(招待講演)
  • Year and Date: 2011-10-26
• [Presentation] Mechanism and reconstitution of germ cell specification in mice (2011)
  • Author(s): Mitinori Saitou
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(招待講演)
  • Year and Date: 2011-09-22
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2011)
  • Author(s): Saitou, M
  • Organizer: Stem Cells in Development and Disease Max-Delbruck-Center
  • Place of Presentation: Berlin-Buch, Germany
  • Year and Date: 2011-09-12
• [Presentation] Mechanism and reconstitution in vitro of germ cell specification in mice (2011)
  • Author(s): Mitinori Saitou
  • Organizer: Stem Cells in Development and Disease
  • Place of Presentation: Max-Delbruck-Center, Berlin-Bucn(招待講演)
  • Year and Date: 2011-09-12
• [Presentation] Germ cell specification in mice : signalling, transcription regulation, and epigenetic reprogramming (2011)
  • Author(s): Mitinori Saitou
  • Organizer: Heidelberg-Kyoto Joint Symposium
  • Place of Presentation: Heidelberg University(招待講演)
  • Year and Date: 2011-07-22
• [Presentation] Understanding and reconstituting mammalian germ cell specification (2011)
  • Author(s): Mitinori Saitou
  • Organizer: 44th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: Okinawa Convention Center(招待講演)
  • Year and Date: 2011-05-21
• [Presentation] Single-cell Transcriptome Profiling in Development : from Pathway Analysis to resonstitution (2011)
  • Author(s): Mitinori Saitou
  • Organizer: The 5^<th> International Workshop on Approaches to Single-Cell Analysis
  • Place of Presentation: 東京大学武田ホール
  • Year and Date: 2011-03-04
• [Presentation] 生殖細胞形成過程の解析と再構成:ゲノム情報再編機構を如何に研究するか? (2011)
  • Author(s): 斎藤通紀
  • Organizer: 岡崎統合バイオサイエンスセンター10周年記念シンポジウム
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2011-02-11
• [Presentation] Global transcription profiling of complex biological processes of cellular differentiation at the single-cell resolution (2010)
  • Author(s): Kazuki Kurimoto, Mitinori Saitou
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-19
• [Presentation] 生殖細胞の形成機構とその再構成 (2010)
  • Author(s): 斎藤通紀
  • Organizer: 第26回 Wakoワークショップ
  • Place of Presentation: The Grand Hall(品川グランドセントラルタワー3F)
  • Year and Date: 2010-11-26
• [Presentation] Specification of germ cell fate in mice in vivo and in vitro (2010)
  • Author(s): Mitinori Saitou
  • Organizer: International Symposium on "Epigenome Network, Development and Reprogramming of Germ cells"
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 2010-11-23
• [Presentation] Germ cell specification in mice in vivo and in vitro (2010)
  • Author(s): Saitou, M
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells
  • Place of Presentation: Cold Spring Harbor Laboratory, New York, USA
  • Year and Date: 2010-10-05
• [Presentation] Germ cell specification in mice in vivo and in vitro. (2010)
  • Author(s): Saitou, M.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells Cold Spring Harbor Laboratory
  • Place of Presentation: New York
  • Year and Date: 2010-10-05
• [Presentation] Germ cell specification in mice in vivo and in vitro (2010)
  • Author(s): Mitinori Saitou
  • Organizer: Cold Spring Harbor Laboratory Meeting on Germ Cells
  • Place of Presentation: Cold Spring Harbor Laboratory, New York
  • Year and Date: 2010-10-05
• [Presentation] Germ cell specification in mice in vivo and in vitro (2010)
  • Author(s): Mitinori Saitou
  • Organizer: 2010 Cold Spring Harbor Asia Conference : Molecular Switches & Genome Function in Stem Cells & Development
  • Place of Presentation: Cold Spring Harbor Asia Conference Centre (Dushu Lake Conference Hotel F1)
  • Year and Date: 2010-09-22
• [Presentation] 生殖系列の起源と特性、その再構成 (2010)
  • Author(s): 斎藤通紀
  • Organizer: 第11回 運動器科学研究会
  • Place of Presentation: 万平ホテル軽井沢
  • Year and Date: 2010-09-10
• [Presentation] 生殖系列の起源と特性及びその再構成 (2010)
  • Author(s): 斎藤通紀
  • Organizer: 第1回 Molecular Cardiovascular Conference II
  • Place of Presentation: キロロ"ホテルピアノ"(北海道余市郡赤井川村)
  • Year and Date: 2010-09-04
• [Presentation] A signaling principle for the specification of the germ cell lineage in mice (2010)
  • Author(s): Mitinori Saitou
  • Organizer: Joint Meeting of Society for Developmental Biology and Japanese Society for Developmental Biology
  • Place of Presentation: Albuquerque
  • Year and Date: 2010-08-08
• [Presentation] 生殖系列の起源と特性、その再構成 (2010)
  • Author(s): 斎藤通紀
  • Organizer: 第16回 成人病の病因・病態の解明に関する研究会
  • Place of Presentation: 軽井沢プリンスホテル
  • Year and Date: 2010-07-04
• [Presentation] 生殖系列の起源と特性、その再構成 (2010)
  • Author(s): 斎藤通紀
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] Germ cell specification in vivo and in vitro (2010)
  • Author(s): Saitou, M
  • Organizer: ISSCR 8^<th> Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2010-06-18
• [Presentation] Germ cell specification in vivo and in vitro. (2010)
  • Author(s): Saitou, M.
  • Organizer: ISSCR 8th Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-06-18
• [Presentation] Germ cell specification in vivo and in vitro (2010)
  • Author(s): Mitinori Saitou
  • Organizer: ISSCR 8^<th> Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2010-06-18
• [Presentation] 生殖系列の起源と特性、その再構成 (2010)
  • Author(s): 斎藤通紀
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-19
• [Presentation] 生殖系列の起源と特性、その再構成 (2010)
  • Author(s): 斎藤通紀
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場・広島
  • Year and Date: 2010-03-18
• [Presentation] 生殖系列の起源と特性、その再構成 (2009)
  • Author(s): 斎藤通紀
  • Organizer: 日本発生生物学会 秋期シンポジウム
  • Place of Presentation: 東レ総合研修センター・三島
  • Year and Date: 2009-11-28
• [Presentation] 始原生殖細胞の形成機構とその再構成 (2009)
  • Author(s): 斎藤通紀
  • Organizer: 文部科学省・科学研究費補助金・特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」第2回 公開シンポジウム
  • Place of Presentation: コクヨホール・品川
  • Year and Date: 2009-11-27
• [Presentation] 生殖系列の起源と特性、その再構成 (2009)
  • Author(s): 斎藤通紀
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートピアホテル・神戸
  • Year and Date: 2009-10-22
• [Presentation] Specification of the germ cell lineage in mice. (2009)
  • Author(s): Mitinori Saitou
  • Organizer: Joint Cold Spring Harbor Laboratory/Wellcome Trust Conference Mouse Genetics & Genomics : Development & Disease
  • Place of Presentation: Wellcome Trust Conference Centre, Hinxton, Cambridge, UK
  • Year and Date: 2009-09-04
• [Presentation] A signaling principle for the specification of the germ cell lineage in mice (2009)
  • Author(s): Mitinori Saitou
  • Organizer: International Conference on Germ Cell Development-Function&19^<th> Annual Meeting of ISSRF
  • Place of Presentation: Bangalore
  • Year and Date: 2009-01-22
• [Presentation] A signaling principle for the specification of germ cell fate in mice (2008)
  • Author(s): Mitinori Saitou
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] A signaling principle for the specification of germ cell fate in mice (2008)
  • Author(s): Mitinori Saitou
  • Organizer: International Symposium on Stem Cells, Epigenetics, and Development
  • Place of Presentation: Taipei
  • Year and Date: 2008-09-27
• [Presentation] Single-cell cDNA microarray analysis of cell fate specification in mammalian development (2008)
  • Author(s): Mitinori Saitou
  • Organizer: 3^<rd> International Workshop on Approaches to Single-Cell Analysis
  • Place of Presentation: Zurich
  • Year and Date: 2008-09-12
• [Presentation] A signaling principle for the specification of germ cell fate in mice (2008)
  • Author(s): Mitinori Saitou
  • Organizer: 2008 Santa Cruz Developmental Biology Meeting
  • Place of Presentation: UC Santa Cruz
  • Year and Date: 2008-06-28
• [Presentation] A signaling principle for the specification of germ cell fate in mice (2008)
  • Author(s): Mitinori Saitou
  • Organizer: 6^<th> ISSCR (International Society for Stem Cell Research) Annual Meeting
  • Place of Presentation: Philadelphia
  • Year and Date: 2008-06-14
• [Book] Primordial germ cells in mice (2012)
  • Author(s): Saitou, M., and Yamaji, M
  • Total Pages: 4
  • Publisher: CSH Perspectives in Biology
• [Book] 再生医療叢書1「幹細胞」 (2012)
  • Author(s): 林克彦、斎藤通紀
  • Total Pages: 208
  • Publisher: 朝倉書店
• [Book] 日本医師会雑誌、第141巻・第6号 (2012)
  • Author(s): 林克彦、斎藤通紀
  • Publisher: 日本医師会
• [Book] 実験医学増刊vol. 30「幹細胞のメディカルサイエンス」 (2012)
  • Author(s): 林克彦、斎藤通紀
  • Total Pages: 205
  • Publisher: 羊土社
• [Book] A global single-cell cDNA amplification method for quantitative microarray analysis (2011)
  • Author(s): Kurimoto, K., and Saitou, M
  • Publisher: Methods in Molecular Biology
• [Book] A global single-cell cDNA amplification method for quantitative microarray analysis., 687 (2011)
  • Author(s): Kurimoto, K., Saitou, M.
  • Publisher: Methods in Molecular Biology
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/organization-staff/research/doctoral_course/r-003/
• [Remarks]
  • URL: http://step.anat2.med.kyoto-u.ac.jp/
• [Remarks] ホームページ
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1103/
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1103/
• [Remarks]
  • URL: http://www.cdb.riken.jp/en/02_research/0202_creative19.html
• [Remarks]
  • URL: http://www.cdb.riken.jp/en/04_news/articles/080715_Prdml.html
• [Remarks]
  • URL: http://www.cdb.riken.jp/en/04_news/articles/080620_GeneDev.html
• [Patent(Industrial Property Rights)] METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO GERM CELLS (2013)
  • Inventor(s): 斎藤通紀、中木文雄
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2013-03-01
  • Overseas
• [Patent(Industrial Property Rights)] 「多能性幹細胞から生殖細胞への分化誘導方法」 (2013)
  • Inventor(s): 斎藤通紀, 中木文雄
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2013-03-01
  • Overseas
• [Patent(Industrial Property Rights)] METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO GERM CELLS (2010)
  • Inventor(s): 斎藤通紀、林克彦
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-08-13
  • Overseas
• [Patent(Industrial Property Rights)] METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO GERM CELLS (2010)
  • Inventor(s): 斎藤通紀、林克彦
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-08-13
• [Patent(Industrial Property Rights)] METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO GERM CELLS (2010)
  • Inventor(s): 斎藤通紀、林克彦
  • Industrial Property Rights Holder: 国立大学法人京都大学
  • Filing Date: 2010-08-13
  • Overseas