Nuclear transfer for the analysis of the mechanisms of totipotency acquisition in the germline

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062012
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OGURA Atsuo 独立行政法人理化学研究所, 遺伝工学基盤技術室, 室長 (20194524)
• Co-Investigator(Kenkyū-buntansha): KOHDA Takashi 東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)
• Co-Investigator(Renkei-kenkyūsha): ISHINO Fumitoshi 東京医科歯科大学, 難治疾患研究所, 教授 (60159754)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥109,000,000 (Direct Cost: ¥109,000,000); Fiscal Year 2012: ¥24,200,000 (Direct Cost: ¥24,200,000); Fiscal Year 2011: ¥24,200,000 (Direct Cost: ¥24,200,000); Fiscal Year 2010: ¥20,200,000 (Direct Cost: ¥20,200,000); Fiscal Year 2009: ¥20,200,000 (Direct Cost: ¥20,200,000); Fiscal Year 2008: ¥20,200,000 (Direct Cost: ¥20,200,000)
• Keywords: 核移植 / 全能性 / 生殖系列 / エピジェネティクス / 生殖細胞 / 胚盤胞 / X染色体不活性化 / ゲノム再プログラム化 / マウス / X染色体不活化 / 遺伝子発現 / 再プログラム化

Research Abstract

The germ cell genome can acquire totipotency, the genomic state of early fertilized embryos. To obtain clues to understanding the mechanisms of acquisition of totipotency by the germ cells, the epigenetic characteristics in the embryos reconstructed with different donor cell nuclei during the life cycle were analyzed using the mouse as the model. The results indicated that mature MII oocytes could reprogram all the epigenetic modifications with only some exceptions including a certain histone tail methylation.

Research Products

• [Journal Article] RNAi-mediated Knockdown of <i>Xist</i> Does Not Rescue the Impaired Development of Female Cloned Mouse Embryos (2013)
  • Author(s): Oikawa, et al.
  • Journal Title: Journal of Reproduction and Development Volume: 59 Issue: 3 Pages: 231-237
  • DOI: 10.1262/jrd.2012-195
  • NAID: 10031184751
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] High osmolality vitrification : A new method for the simple and temperature-permissive cryopreservation of mouse embryos. (2013)
  • Author(s): Mochida, et al.
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e49316-e49316
  • DOI: 10.1371/journal.pone.0049316
  • Peer Reviewed
• [Journal Article] Recent advancements in cloning by somatic cell nuclear transfer. (2013)
  • Author(s): Ogura, et al.
  • Journal Title: Philos Trans R Soc Lond B Biol Sci Volume: 368 Issue: 1609 Pages: 20110329-20110329
  • DOI: 10.1098/rstb.2011.0329
  • Peer Reviewed
• [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos. (2012)
  • Author(s): Nakamura T, Liu Y-J, Nakashima H, Umehara H, Inoue K, Matoba S, Tachibana M, Ogura A, Shinkai Y, Nakano T
  • Journal Title: Nature Volume: 486 Pages: 415-419
  • Peer Reviewed
• [Journal Article] Efficient production of offspring from Japanese wild-derived strains of mice (Mus musculus molossinus) by improved assisted reproductive technologies. (2012)
  • Author(s): Hasegawa A, Mochida K, Matoba S, Yonezawa K, Ohta A, Watanabe G, Taya K, Ogura A
  • Journal Title: Biol Reprod Volume: 86: 86: 167 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Optimization of a protocol for cryopreservation of mouse spermatozoa using cryotubes. (2012)
  • Author(s): Hasegawa A, Yonezawa K, Ohta A, Mochida K, Ogura A
  • Journal Title: J Reprod Dev Volume: 58 Pages: 156-161
  • NAID: 10030407685
  • Peer Reviewed
• [Journal Article] Recent advancements in cloning by somatic cell nuclear transfer. (2011)
  • Author(s): Ogura A, Inoue K, Wakayama T
  • Journal Title: Philos Trans RSoc Lond B Biol Sci Volume: 368 Pages: 329-329
  • URL: http://dx.doi.org/10.1098/rstb.2011.0329
  • Peer Reviewed
• [Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos. (2011)
  • Author(s): Matoba S, Inoue K, Kohda T, Sugimioto M, Mizutani E, Ogonuki N, Nakamura T, Abe K, Nakano T, Ishino F, and Ogura A
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 20621-20626
  • Peer Reviewed
• [Journal Article] Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect. (2011)
  • Author(s): Kohda T, Ogonuki N, Inoue K, Furuse T, Kaneda H, Suzuki T, Kaneko-Ishino T, Wakayama T, Wakana S, Ogura A, Ishino F
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 282-288
  • Peer Reviewed
• [Journal Article] In vitro production of functional sperm in cultured neonatal mouse testes. (2011)
  • Author(s): Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, & Ogawa T
  • Journal Title: Nature Volume: 471 Pages: 504-507
  • Peer Reviewed
• [Journal Article] Birth of normal mice following round spermatid injection without artificial oocyte activation. (2011)
  • Author(s): Ogonuki N, Inoue K, Ogura A
  • Journal Title: J Reprod Dev Volume: 57 Pages: 534-538
  • NAID: 10029593452
  • Peer Reviewed
• [Journal Article] Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult animals. (2011)
  • Author(s): Matoba S, Ogura A
  • Journal Title: Biol Reprod Volume: 84 Pages: 631-838
  • Peer Reviewed
• [Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos (2011)
  • Author(s): Matoba S, et al
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Issue: 51 Pages: 20621-20626
  • DOI: 10.1073/pnas.1112664108
  • Peer Reviewed
• [Journal Article] Birth of Normal Mice Following Round Spermatid Injection Without Artificial Oocyte Activation (2011)
  • Author(s): Ogonuki N, et al
  • Journal Title: Journal of Reproduction and Development Volume: 57 Issue: 4 Pages: 534-538
  • DOI: 10.1262/jrd.11-008M
  • NAID: 130000677045
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect (2011)
  • Author(s): Kohda T, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Issue: 2 Pages: 282-288
  • DOI: 10.1016/j.bbrc.2011.05.133
  • Peer Reviewed
• [Journal Article] Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult animals. (2011)
  • Author(s): Matoba S, Ogura A
  • Journal Title: Biology of Reproduction Volume: VOL.84(In press)
  • Peer Reviewed
• [Journal Article] The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice. (2010)
  • Author(s): Ogonuki N, Mori1 M, Shinmen A, Inoue K, Mochida K, Ohta A, Ogura A
  • Journal Title: PLoS ONE Volume: 5(6) Issue: 6 Pages: e11062-e11062
  • DOI: 10.1371/journal.pone.0011062
  • Year and Date: 2010-06-11
  • Peer Reviewed
• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. (2010)
  • Author(s): Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A
  • Journal Title: Science Volume: 330 Pages: 496-499
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine. (2010)
  • Author(s): Honda A, Hirose M, Hatori M, Matoba S, Miyoshi H, Inoue K, Ogura A
  • Journal Title: J Biol Chem Volume: 285 Pages: 31362-31369
  • Peer Reviewed
• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. (2010)
  • Author(s): Inoue, K., Kohda, T., (14名省略), Ogura, A.
  • Journal Title: Science 330 Pages: 496-499
  • Peer Reviewed
• [Journal Article] The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice. (2010)
  • Author(s): Ogonuki N., 6名省略, Ogura, A.
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. (2010)
  • Author(s): Inoue K, et al.
  • Journal Title: Science Volume: VOL.330 Pages: 496-499
  • Peer Reviewed
• [Journal Article] The effect on intracytoplasmic sperm injection outcome of genotype, male germ cell stage and freeze-thawing in mice. (2010)
  • Author(s): Ogonuki N, et al.
  • Journal Title: PLoS One Volume: VOL.5
  • Peer Reviewed
• [Journal Article] Sex-reversed somatic cell cloning in the mouse. (2009)
  • Author(s): Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, Ogura A
  • Journal Title: Reprod Dev Volume: 55 Pages: 566-569
  • NAID: 10025616710
  • Peer Reviewed
• [Journal Article] Embryonic rather than extraembryonic tissues have more impact on the development of placental hyperplasia in cloned mice. (2009)
  • Author(s): Miki H, Wakisaka N, Inoue K, Ogonuki N, Mori M, Kim JM, Ohta A, Ogura A
  • Journal Title: Placenta Volume: 30 Pages: 543-546
  • Peer Reviewed
• [Journal Article] Changes in allele-specific association of histone modifications at the imprinting control regions during mouse preimplantation development. (2009)
  • Author(s): Kim J-M, Ogura A
  • Journal Title: Genesis Volume: 47 Pages: 611-616
  • Peer Reviewed
• [Journal Article] A high-speed congenic strategy using first-wave male germ cells. (2009)
  • Author(s): Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, Mise N, Mekada K, Yoshiki A, Abe K, Kurihara H, Wakana S, Ogura A
  • Journal Title: PLoS One Volume: 4(3) Issue: 3 Pages: e4943-e4943
  • DOI: 10.1371/journal.pone.0004943
  • Peer Reviewed
• [Journal Article] Basic FGF and Activin/Nodal but not LIF signaling sustain undifferentiated status of rabbit embryonic stem cells. (2009)
  • Author(s): Honda A, Hirose M, Ogura A
  • Journal Title: Exp Cell Res Volume: 315 Pages: 2033-2042
  • Peer Reviewed
• [Journal Article] Efficient production of androgenetic embryos by round spermatid injection. (2009)
  • Author(s): Miki H, Hirose M, Ogonuki N, Inoue K, Kezuka F, Honda A, Mekada K, Hanaki K, Iwafune H, Yoshiki A, Ishino F, Ogura A
  • Journal Title: Genesis Volume: 47 Pages: 155-60
  • Peer Reviewed
• [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries. (2009)
  • Author(s): Honda A, Hirose M, Inoue K, Hiura H, Miki H, Ogonuki N, Sugimoto M, Abe K, Kanatsu-Shinohara M, Kono T, Shinohara T, Ogura A
  • Journal Title: Int J Dev Biol Volume: 53 Pages: 605-613
  • Peer Reviewed
• [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries. (2009)
  • Author(s): Honda, A., 10名省略, Ogura, A.
  • Journal Title: Int.J.Dev.Biol. 53 Pages: 605-613
  • Peer Reviewed
• [Journal Article] Sex-reversed somatic cell cloning in the mouse. (2009)
  • Author(s): Inoue, K., Ogonuki, N., Mekada, K., Yoshiki, A., Sado, T., Ogura, A.
  • Journal Title: J.Reprod.Dev. 55 Pages: 566-569
  • NAID: 10025616710
  • Peer Reviewed
• [Journal Article] Sex-reversed somatic cell cloning in the mouse (2009)
  • Author(s): Inoue K et al.
  • Journal Title: J.Reprod.Dev. 55 Pages: 566-569
  • Peer Reviewed
• [Journal Article] Embryonic rather than extraembryonic tissues have more impact on the development of placental hyperplasia in cloned mice (2009)
  • Author(s): Miki H et al.
  • Journal Title: Placenta 30 Pages: 543-546
  • Peer Reviewed
• [Journal Article] Changes in allele-specific association of histone modifications at the imprinting control regions during mouse preimplantation development (2009)
  • Author(s): Kim J-M, Ogura A
  • Journal Title: Genesis 47 Pages: 611-616
  • Peer Reviewed
• [Journal Article] Stable ES cell lines in rabbits - potential small animal models for human ES cell research. (2008)
  • Author(s): Honda A, Hirose M, Inoue K, Ogonuki N, Miki H, Shimozawa N, Hatori M, Shimizu N, Murata T, Hirose H, Katayama K, Wakisaka N, Miyoshi H, Yokoyama KK, Sankai T, Ogura A
  • Journal Title: Reprod Biomed Online Volume: 17 Pages: 706-715
  • Peer Reviewed
• [Journal Article] Ultrastructure of placental hyperplasia in mice: Comparison of placental phenotypes with three different etiologies. (2008)
  • Author(s): Wakisaka N, Inoue K, Ogonuki N, Miki H, Sekita Y, Hanaki K, Akatsuka K, Kaneko-Ishino T, Ishino F, Ogura A
  • Journal Title: Placenta Volume: 29 Pages: 753-759
  • NAID: 130007022881
  • Peer Reviewed
• [Journal Article] Role of retrotransposon-derived imprinted gene, Rtl1, in the feto-maternal interface of mouse placenta. (2008)
  • Author(s): Sekita Y, Wagatsuma H, Nakamura K, Ono R, Kagami M, Wakisaka N, Hino T, Suzuki-Migishima R, Kohda T, Ogura A, Ogata T, Yokoyama M, Kaneko-Ishino T, Ishino F
  • Journal Title: Nat Genet Volume: 40 Pages: 243-248
  • Peer Reviewed
• [Journal Article] Ultrastructure of placental hyperplasia in mice : Comparison of placental phenotypes with three different etiologies (2008)
  • Author(s): Wakisaka, et al.
  • Journal Title: Placenta 29 Pages: 753-759
  • Peer Reviewed
• [Journal Article] RNAi-mediated knockdown of Xist does not rescue the impaired development of female cloned mouse embryos.
  • Author(s): Oikawa M, Matoba S, Inoue K, Kamimura S, Hirose M, Ogonuki N, Shiura H, Sugimito M, Abe K, Ishino F, Ogura A
  • Journal Title: J Reprod Dev Volume: (in press)
  • NAID: 10031184751
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine.
  • Author(s): Honda, A., 5名省略 , Ogura, A.
  • Journal Title: J.Biol.Chem. in press
  • Peer Reviewed
• [Journal Article] Efficient production of androgenetic embryos by round spermatid injection
  • Author(s): Miki, et al.
  • Journal Title: Genesis (印刷中)
  • Peer Reviewed
• [Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse obaries
  • Author(s): Honda, et al.
  • Journal Title: Int J Dev Biol (印刷中)
  • Peer Reviewed
• [Presentation] Analysis of the dynamics of imprinted X chromosome inactivation in mice by nuclear transfer (2012)
  • Author(s): Ogura A, Oikawa M, Matoba S, Inoue K
  • Organizer: The 9th Annual Conference of the Asian Reproductive Biotechnology Society Manila Philippine
  • Place of Presentation: Manila Philippine
  • Year and Date: 2012-10-24
• [Presentation] Nuclear transfer for the study of X chromosome inactivation in mice (2012)
  • Author(s): Ogura A, Oikawa M, Inoue K
  • Organizer: Mouse Molecular Genetics California USA
  • Place of Presentation: California USA
  • Year and Date: 2012-10-04
• [Presentation] Nuclear transfer for the study of X chromosome inactivation in mice (2012)
  • Author(s): Atsuo Ogura
  • Organizer: Mouse Molecular Genetics
  • Place of Presentation: Pacific Grove, CA, USA(招待講演)
  • Year and Date: 2012-10-04
• [Presentation] Nuclear transfer for the study of epigenetic dynamics in mammalian development (2012)
  • Author(s): Ogura A
  • Organizer: The 84th Annual Meeting of the Genetics Society of Japan Fukuoka
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-09-24
• [Presentation] Development of ES/iPS cell research using rabbits (2012)
  • Author(s): Honda A, Hirose M, Hatori M, Ogura A
  • Organizer: SRD-NIAS the 2nd Japan-Czech Joint Symposium Tokyo
  • Place of Presentation: Tokyo
  • Year and Date: 2012-09-10
• [Presentation] X-chromosome inactivation status in cloned mouse embryos Epigenetic Memory (2012)
  • Author(s): Ogura A
  • Organizer: The Company of Biologists Workshop Steyning UK
  • Place of Presentation: Steyning UK
  • Year and Date: 2012-06-26
• [Presentation] Transportation of frozen and unfrozen materials (2012)
  • Author(s): Mochida K, Ogura A
  • Organizer: EMMA cryopreservation workshop Spain Madrid
  • Place of Presentation: Spain Madrid,
  • Year and Date: 2012-05-08
• [Presentation] ICSI and its practical applications (2012)
  • Author(s): Ogura A
  • Organizer: EMMA Cryopreservation Workshop Madrid Spain
  • Place of Presentation: Madrid Spain
  • Year and Date: 2012-05-08
• [Presentation] Critical involvement of aberrant X chromosome inactivation in the defective postimplantation development of cloned embryos (2011)
  • Author(s): 小倉淳郎
  • Organizer: International Meeting for Evolution of Reproductive Biology and Task of Frontiers
  • Place of Presentation: 県民情報交流センター・アイーナ(盛岡市)(招待講演)
  • Year and Date: 2011-09-14
• [Presentation] Improvement of somatic cell nuclear transfer in mice : Genetic and epigenetic approaches. (2010)
  • Author(s): Ogura A, Inoue K
  • Organizer: Czeck-Japan Joint Symposium for Animal Reproduction, "From Gametes to Stem Cells
  • Place of Presentation: Liblice Castle, Czech Republic
  • Year and Date: 2010-09-21
• [Presentation] Improvement of somatic cell nuclear transfer in mice : Genetic and epigenetic approaches (2010)
  • Author(s): Atsuo Ogura, Kimiko Inoue
  • Organizer: Czeck-Japan Joint Symposium for Animal Reproduction, "From Gametes to Stem Cells"
  • Place of Presentation: Liblice Castle, Czech Republic
  • Year and Date: 2010-09-21
• [Presentation] Recent technical advancements of nuclear transfer in the mouse. (2010)
  • Author(s): Ogura A.
  • Organizer: 4th AFLAS Congress Meeting/5th AMMRA Annual Meeting/11th CSLAS Annual Meeting
  • Place of Presentation: Taipei, Taiwan
• [Presentation] 雄性生殖細胞の受精能とゲノム刷込み (2009)
  • Author(s): 小倉淳郎
  • Organizer: 農業生物資源研究所 生殖機構研究シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-12-04
• [Presentation] 雄性生殖細胞の受精能とゲノム刷り込み (2009)
  • Author(s): 小倉淳郎
  • Organizer: 農業生物資源研究所 生殖機構研究シンポジウム
  • Place of Presentation: 東京
• [Presentation] Micro-insemination and nuclear transfer using male germ cells. (2008)
  • Author(s): Ogura A.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Hawaii, U.S.A.
  • Year and Date: 2008-05-24
• [Presentation] Plenary lecture : Microinsemination and nuclear transfer using male germ cells (2008)
  • Author(s): Ogura, A
  • Organizer: 1st World Congress on Reproductive Biology
  • Place of Presentation: Kona, U.S.A
  • Year and Date: 2008-05-24
• [Book] Cryopreservation and Distribution of Mouse Models:Challenges and Solutions (2013)
  • Author(s): Atsuo Ogura
  • Publisher: Springer(印刷中)
• [Book] 「核移植技術:Xist の機能抑制による体細胞クローン作出効率の改善法」細胞工学 (2012)
  • Author(s): 的場章悟,井上貴美子,小倉淳郎
• [Book] 「核移植クローン技術の未来は?」細胞工学 (2012)
  • Author(s): 小倉淳郎
• [Book] 細胞培養プロトコール(中村幸夫編) (2012)
  • Author(s): 廣瀬美智子,小倉淳郎
  • Publisher: 羊土社
• [Book] 遺伝子の導入法とノックアウト法 生命の誕生に向けて(第二版) (2011)
  • Author(s): 小倉 淳郎, 井上 貴美子
  • Publisher: 近代出版 東京
• [Book] 顕微授精と体外受精の比較論卵子学 (総編集者 森 崇英) (2011)
  • Author(s): 小倉 淳郎
  • Publisher: 京都大学学術出版会 京都
• [Book] 卵子学 (2011)
  • Author(s): 小倉淳郎
  • Publisher: 京都大学学術出版会
• [Book] 遺伝子の導入法とノックアウト法(生命の誕生に向けて) (2011)
  • Author(s): 小倉淳郎、井上貴美子
  • Publisher: 近代出版
• [Book] 生命の誕生に向けて (2011)
  • Author(s): 小倉淳郎、井上貴美子
  • Total Pages: 300
  • Publisher: 近代出版
• [Book] 活性X 染色体からのXist 遺伝子の発現の抑制はマウス体細胞クローンの効率を改善するライフサイエンス新着論文レビュー Vol.1190 (2010)
  • Author(s): 井上 貴美子, 小倉 淳郎
• [Book] 新生仔マウス卵巣から分離された莢膜幹細胞と卵子の特徴について 日本生殖内分泌学会雑誌 Vol.15 (2010)
  • Author(s): 本多 新, 小倉 淳郎
• [Book] 核移植によるリプログラミング 細胞 Vol.42 12 (2010)
  • Author(s): 小倉 淳郎, 井上 貴美子
• [Book] 体細胞クローン動物のエピジェネティクスと表現型 日本胚移植学雑誌Vol.31 2 (2009)
  • Author(s): 小倉 淳郎
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/kougaku/
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/kougaku/
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/kougaku/
• [Remarks] 「クローンマウス出生率10倍に」(日経産業新聞20100917・研究室のホームページを開設・更新した
  • URL: http://www.brc.riken.go.jp/lab/kougaku/
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/research/lab/brc/engineering/index.html
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/kougaku/
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/research/lab/brc/engineering/index.html
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/kougaku/
• [Remarks]
  • URL: http://www.riken.jp/r-world/research/lab/brc/engineering/index.html
• [Patent(Industrial Property Rights)] クローン動物の作出方法 (2010)
  • Inventor(s): 小倉淳郎,井上貴美子,石野史敏,幸田尚,澤井 健
  • Industrial Property Rights Holder: 小倉淳郎,井上貴美子,石野史敏,幸田尚,澤井 健
  • Filing Date: 2010-09-03