How to enhance the nuclear reprogramming by oocyte cytoplasm and analysis the mechanisum

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 20062015
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Yamanashi (2012); The Institute of Physical and Chemical Research (2008-2011)
• Principal Investigator: WAKAYAMA Teruhiko 山梨大学, 大学院・医学工学総合研究部, 教授 (40360672)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥100,000,000 (Direct Cost: ¥100,000,000); Fiscal Year 2012: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2011: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2010: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2009: ¥20,000,000 (Direct Cost: ¥20,000,000); Fiscal Year 2008: ¥20,000,000 (Direct Cost: ¥20,000,000)
• Keywords: 応用動物 / 再生医学 / 獣医学 / 畜産学 / 発生分化 / 発生・分化 / 初期化 / リプログラミング / 核移植 / クローン動物 / 卵子

Research Abstract

We could demonstrate several possibilities. 1. The cloning success rate was improved up to 10% by HDACis. 2. Health cloned mice were born from frozen dead mouse, which preserved 16 years under -20C. 3. Over six hundreds cloned mice were born from one donor mouse by repeating nuclear transfer up to 27 times.

Research Products

• [Journal Article] Nicotinamide: a Class III HDACi Delays In Vitro Aging of Mouse Oocytes (2013)
  • Author(s): Lee AR, Kishigami S, Amano T, Matsumoto K, Wakayama T, Hosoi Y
  • Journal Title: J Reprod Dev Volume: (Epub ahead of print)
  • NAID: 10031184752
  • Peer Reviewed
• [Journal Article] Successful Serial Recloning in the Mouse over Multiple Generations (2013)
  • Author(s): Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T
  • Journal Title: Cell Stem Cell Volume: 12(3) (Cover article of issue) Pages: 293-7
  • Peer Reviewed
• [Journal Article] Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development. (2013)
  • Author(s): Takase M, Iida R, Maruya M, Sakaue-Sawano A, Miyawaki A, Wakayama T, Nishigami S, Fagarasan S, Kanagawa O.
  • Journal Title: International immunology Volume: 25 Issue: 3 Pages: 145-156
  • DOI: 10.1093/intimm/dxs095
  • Peer Reviewed
• [Journal Article] Successful Serial Recloning in the Mouse over Multiple Generations. (2013)
  • Author(s): Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T.
  • Journal Title: Cell Stem Cell Volume: 12 Issue: 3 Pages: 293-297
  • DOI: 10.1016/j.stem.2013.01.005
  • Peer Reviewed
• [Journal Article] Nuclear transferred embryonic stem cell for analysis of B1 B-lymphocyte development (2012)
  • Author(s): Takase M, Iida R, Maruya M, Sakaue-Sawano A, Miyawaki A, Wakayama T, Nishigami S, Fagarasan S, Kanagawa O
  • Journal Title: Int Immunol. Int Immunol Volume: 25(3) Pages: 145-156
  • Peer Reviewed
• [Journal Article] Gene Expression Profile Normalization in Cloned Mice by Trichostatin A Treatment (2012)
  • Author(s): Kohda T, Kishigami S, Kaneko-Ishino T, Wakayama T, Ishino F
  • Journal Title: Cell Reprogram Volume: 14 Pages: 45-55
  • Peer Reviewed
• [Journal Article] Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones (2012)
  • Author(s): Mizutani E, Yamagata K, Ono T, Akagi S, Geshi M, Wakayama T
  • Journal Title: Dev Biol. 2012 Volume: 364 Pages: 56-65
  • Peer Reviewed
• [Journal Article] Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy (2012)
  • Author(s): Yamagata K, Iwamoto D, Terashita Y, Li C, Wakayama S, Hayashi-Takanaka Y, Kimura H, Saeki K, Wakayama T
  • Journal Title: PLoS One Volume: 7(2)
  • Peer Reviewed
• [Journal Article] Latrunculin A Can Improve the Birth Rate of Cloned Mice and Simplify the Nuclear Transfer Protocol by Gently Inhibiting Actin Polymerization (2012)
  • Author(s): Terashita Y, Wakayama S, Yamagata K, Li C, Sato E, Wakayama T
  • Journal Title: Biol Reprod Volume: 86 Pages: 1801-6
  • NAID: 130005457607
  • Peer Reviewed
• [Journal Article] Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent (2012)
  • Author(s): Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T
  • Journal Title: PLoS One Volume: 7
  • NAID: 130005457584
  • Peer Reviewed
• [Journal Article] Latrunculin A can improve the birth rate of cloned mice and simplify the nuclear transfer protocol by gently inhibiting actin polymerization. (2012)
  • Author(s): Terashita Y, Wakayama S, Yamagata K, Li C, Sato E, Wakayama T.
  • Journal Title: Biology of Reproduction Volume: 86 Issue: 6 Pages: 1-6
  • DOI: 10.1095/biolreprod.111.098764
  • Peer Reviewed
• [Journal Article] Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent. (2012)
  • Author(s): Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T
  • Journal Title: Plos One Volume: 7 Issue: 10 Pages: 1-9
  • DOI: 10.1371/journal.pone.0047512
  • Peer Reviewed
• [Journal Article] Gene Expression Profile Normalization in Cloned Mice by Trichostatin A Treatment (2012)
  • Author(s): Kohda T, et al.
  • Journal Title: Cell Reprogramming Volume: (in press) Issue: 1 Pages: 45-55
  • DOI: 10.1089/cell.2011.0062
  • Peer Reviewed
• [Journal Article] Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones (2012)
  • Author(s): Mizutani E, et al.
  • Journal Title: Developmental Biology Volume: (in press) Issue: 1 Pages: 56-65
  • DOI: 10.1016/j.ydbio.2012.01.001
  • Peer Reviewed
• [Journal Article] Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development (2011)
  • Author(s): Bui HT, Wakayama S, Mizutani E, Park KK, Kim JH, Van Thuan N, Wakayama T
  • Journal Title: Reproduction Volume: 141 Pages: 67-77
  • Peer Reviewed
• [Journal Article] Choice of random rather than imprinted X inactivation in female embryonic stem cell-derived extra-embryonic cells (2011)
  • Author(s): Murakami K, Araki K, Ohtsuka S, Wakayama T, Niwa H
  • Journal Title: Development Volume: 138 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Generation of Healthy Cloned Mice using Enucleated Cryopreserved Oocytes (2011)
  • Author(s): Hirata S, Fukasawa H, Wakayama S, Wakayama T, Hoshi K
  • Journal Title: Cellular Reprogramming Volume: 13 Pages: 7-11
  • Peer Reviewed
• [Journal Article] Analysis of proteomic profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic blastocysts (2011)
  • Author(s): Cui, XS., Shen, XH., Lee CK., Kang, YK., Wakayama, T. Kim NH
  • Journal Title: Stem Cell Discovery Volume: 1 Pages: 1-15
  • Peer Reviewed
• [Journal Article] Effect of Fluorescent Mercury Light Irradiation on In Vitro and In Vivo Development of Mouse Oocytes after Parthenogenetic Activation or Sperm Microinjection (2011)
  • Author(s): Terashita Y, Li C, Yamagata K, Sato E, Wakayama T
  • Journal Title: J Reprod Dev Volume: 57(Cover article of issue) Pages: 564-571
  • NAID: 10029878128
  • Peer Reviewed
• [Journal Article] Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect (2011)
  • Author(s): Kohda T, Ogonuki N, Inoue K, Furuse T, Kaneda H, Suzuki T, Kaneko-Ishino T, Wakayama T, Wakana S, Ogura A, Ishino F
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 282-288
  • Peer Reviewed
• [Journal Article] Intracytoplasmic Sperm Injection with Mouse Spermatozoa Preserved Without Freezing for Six Months Can Lead to Full-Term Development (2011)
  • Author(s): Li C, Mizutani E, Ono T, Terashita Y, Jia XF, Shi HJ, Wakayama T
  • Journal Title: Biol Reprod Volume: 85 Pages: 1183-1190
  • Peer Reviewed
• [Journal Article] Histone Deacetylase Inhibition Improves Activation of Ribosomal RNA Genes and Embryonic Nucleolar Reprogramming in Cloned Mouse Embryos (2011)
  • Author(s): Bui HT, Seo HJ, Park MR, Park JY, Van Thuan N, Wakayama T, Kim JH
  • Journal Title: Biol Reprod Volume: 85 Pages: 1048-1056
  • Peer Reviewed
• [Journal Article] Offspring from intracytoplasmic sperm injection of aged mouse oocytes treated with caffeine or MG132 (2011)
  • Author(s): Ono T, et al.
  • Journal Title: Genesis Volume: 49 Issue: 6 Pages: 460-471
  • DOI: 10.1002/dvg.20756
  • Peer Reviewed
• [Journal Article] Nuclear-cytoplasmic incompatibility and inefficient development of pig-mouse cytoplasmic hybrid embryos (2011)
  • Author(s): Amarnath D, et al.
  • Journal Title: Reproduction Volume: 142 Issue: 2 Pages: 295-307
  • DOI: 10.1530/rep-11-0044
  • Peer Reviewed
• [Journal Article] Effect of Fluorescent Mercury Light Irradiation on In Vitro and In Vivo Development of Mouse Oocytes after Parthenogenetic Activation or Sperm Microinjection (2011)
  • Author(s): Terashita Y, et al.
  • Journal Title: Journal of Reproduction and Development Volume: 57 Issue: 5 Pages: 564-571
  • DOI: 10.1262/jrd.11-015H
  • NAID: 10029878128
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] The novel use of modified pig zygotic medium for the efficient culture of the preimplantation mouse embryos (2011)
  • Author(s): Amarnath D, et al.
  • Journal Title: Theriogenology Volume: 76 Issue: 9 Pages: 1639-1646
  • DOI: 10.1016/j.theriogenology.2011.06.028
  • Peer Reviewed
• [Journal Article] Histone Deacetylase Inhibition Improves Activation of Ribosomal RNA Genes and Embryonic Nucleolar Reprogramming in Cloned Mouse Embryos (2011)
  • Author(s): Bui HT, et al.
  • Journal Title: Biology of Reproduction Volume: 85 Issue: 5 Pages: 1048-1056
  • DOI: 10.1095/biolreprod.110.089474
  • Peer Reviewed
• [Journal Article] Intracytoplasmic Sperm Injection with Mouse Spermatozoa Preserved Without Freezing for Six Months Can Lead to Full-Term Development (2011)
  • Author(s): Li C, et al.
  • Journal Title: Biology of Reproduction Volume: 85 Issue: 6 Pages: 1183-1190
  • DOI: 10.1095/biolreprod.111.091827
  • Peer Reviewed
• [Journal Article] Generation of Healthy Cloned Mice Using Enucleated Cryopreserved Oocytes. (2011)
  • Author(s): Hirata S., et al.
  • Journal Title: Cellular Reprogramming Volume: 13 Pages: 7-11
  • Peer Reviewed
• [Journal Article] Choice of random rather than imprinted X inactivation in female embryonic stem cell-derived extra-embryonic cells. (2011)
  • Author(s): Murakami K., et al.
  • Journal Title: Development Volume: 138 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Vitamin A-dependent transcriptional activation of the nuclear factor of activated T cells c1(NFATc1) is critical for the development and survival of B1 cells. (2011)
  • Author(s): Maruya M., et al.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 108 Pages: 722-727
  • Peer Reviewed
• [Journal Article] A role for the elongator complex in zygotic paternal genome demethylation (2010)
  • Author(s): Okada, Y. Yamagata, K. Hong, K. Wakayama, T. Zhang, Y
  • Journal Title: Nature Volume: 463 Pages: 554-558
  • Peer Reviewed
• [Journal Article] Effect of Trichostatin A on chromatin remodeling, histone modifications, DNA replication and transcriptional activity in cloned mouse embryos (2010)
  • Author(s): Bui H.T., Wakayama S., Kishigami S., Kim HJ., Van Thuan N., and Wakayama T
  • Journal Title: Biol Reprod Volume: 83 Pages: 454-463
  • Peer Reviewed
• [Journal Article] DNA Methylation Is Dispensable for the Growth and Survival of the Extraembryonic Lineages (2010)
  • Author(s): Sakaue M, Ohta H, Kumaki Y, Oda M, Sakaide Y, Matsuoka C, Yamagiwa A, Niwa H, Wakayama T, Okano M
  • Journal Title: Curr Biol Volume: 20 Pages: 1452-1457
  • Peer Reviewed
• [Journal Article] Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer (2010)
  • Author(s): Hikichi T., Ohta H., Wakayama S., and Wakayama T
  • Journal Title: Development Volume: 137 Pages: 2841-2847
  • Peer Reviewed
• [Journal Article] Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system (2010)
  • Author(s): Yamagata K, Ueda J, Mizutani E, Saitou M, Wakayama T
  • Journal Title: Dev Biol Volume: 346 Pages: 90-101
  • Peer Reviewed
• [Journal Article] Inhibition of Class IIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice (2010)
  • Author(s): Ono T., Li C., Mizutani E., Terashita Y., Yamagata K., and Wakayama T
  • Journal Title: Biol Reprod Volume: 83 Pages: 929-937
  • Peer Reviewed
• [Journal Article] Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell (2010)
  • Author(s): Kim HR, Han RX, Wakayama T, Park CS, Jin DI
  • Journal Title: Placenta Volume: 31 Pages: 853-859
  • Peer Reviewed
• [Journal Article] Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer (2010)
  • Author(s): Hikichi T., Ohta H., Wakayama S., Wakayama T.
  • Journal Title: Development 137 Pages: 2841-2847
  • Peer Reviewed
• [Journal Article] Inhibition of Class IIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice (2010)
  • Author(s): Ono T., Li C., Mizutani E., Terashita Y., Yamagata K., Wakayama T.
  • Journal Title: Biol Reprod 83 Pages: 929-937
  • Peer Reviewed
• [Journal Article] An Efficient Method for Generating Transgenic Mice Using Na OH-treated Spermatozoa. (2010)
  • Author(s): Li C., et al.
  • Journal Title: Biol.Reprod. Volume: 81 Pages: 331-340
  • Peer Reviewed
• [Journal Article] A role for the elongator complex in zygotic paternal genome demethylation. (2010)
  • Author(s): Okada Y., et al.
  • Journal Title: Nature Volume: 28 Pages: 554-558
  • Peer Reviewed
• [Journal Article] Preservation of sperm within the mouse cauda epididymidis in salt or sugars at room temperature. (2010)
  • Author(s): Ono T., et al.
  • Journal Title: Zygote Volume: 18 Pages: 245-256
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions (T DMRs) residing in mouse pluripotent stem cells. (2010)
  • Author(s): Sato S., et al.
  • Journal Title: Genes Cells Volume: 15 Pages: 607-618
  • Peer Reviewed
• [Journal Article] Histone H3 lysine 27 methylation asymmetry on developmentally-regulated promoters distinguish the first two lineages in mouse preimplantation embryos. (2010)
  • Author(s): Dahl JA., et al.
  • Journal Title: PLoS One. Volume: 5
  • Peer Reviewed
• [Journal Article] Effect of trichostatin A on chromatin remodeling, histone modifications, DNA replication, and transcriptional activity in cloned mouse embryos. (2010)
  • Author(s): Bui T., et al.
  • Journal Title: Biol.Reprod. Volume: 83 Pages: 454-463
  • Peer Reviewed
• [Journal Article] DNA Methylation Is Dispensable for the Growth and Survival of the Ext raembryonic Lineages. (2010)
  • Author(s): Sakaue M., et al.
  • Journal Title: Curr.Biol. Volume: 20 Pages: 1452-1457
  • Peer Reviewed
• [Journal Article] Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system. (2010)
  • Author(s): Yamagata K., et al.
  • Journal Title: Dev.Biol. Volume: 346 Pages: 90-101
  • Peer Reviewed
• [Journal Article] Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer. (2010)
  • Author(s): Hikichi T., et al.
  • Journal Title: Development Volume: 83 Pages: 2841-2847
  • Peer Reviewed
• [Journal Article] Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell. (2010)
  • Author(s): Kim HR., et al.
  • Journal Title: Placenta Volume: 31 Pages: 853-859
  • Peer Reviewed
• [Journal Article] Inhibition of Class IIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice. (2010)
  • Author(s): Ono T., et al.
  • Journal Title: Biol Reprod Volume: 83 Pages: 929-937
  • Peer Reviewed
• [Journal Article] Production of cloned mice from somatic cells, ES cells, and frozen bodies (2010)
  • Author(s): Wakayama S., et al.
  • Journal Title: Methods Enzymol Volume: 476 Pages: 151-169
  • Peer Reviewed
• [Journal Article] How to improve the success rate of mouse cloning technology. (2010)
  • Author(s): Thuan NV., et al.
  • Journal Title: J.Reprod.Dev. Volume: 56 Pages: 20-30
  • Peer Reviewed
• [Journal Article] An Efficient Method for Generating Transgenic Mice Using NaOH-Treated Spermatozoa (2010)
  • Author(s): Li et al.
  • Journal Title: Biol Reprod 82 Pages: 331-340
  • Peer Reviewed
• [Journal Article] Preservation of sperm within the mouse cauda epididymidis in salt or sugars at room temperature (2010)
  • Author(s): Ono et al.
  • Journal Title: Zygote (In press)
  • Peer Reviewed
• [Journal Article] A role for the elongator complex in zygotic paternal genome demethylation (2010)
  • Author(s): Okada et al.
  • Journal Title: Nature 46 Pages: 554-558
  • Peer Reviewed
• [Journal Article] Six-dimensional Live-cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-term Development (2009)
  • Author(s): Yamagata K, Suetsugu R, Wakayama T. Long-term
  • Journal Title: J Reprod Dev Volume: 55 Pages: 343-350
  • Peer Reviewed
• [Journal Article] Effect of Vanadate on the Chromatin Configuration in Pig GV-oocytes (2009)
  • Author(s): Bui HT, Hwang KC, Kim JH, VAN Thuan N, Wakayama T, Miyano T
  • Journal Title: J Reprod Dev Volume: 55 Pages: 367-372
  • NAID: 10025194108
  • Peer Reviewed
• [Journal Article] A signaling principle for the specification of the germ cell lineage in mice (2009)
  • Author(s): Ohinata Y, Ohta H, Shigeta M, Yamanaka K, Wakayama T, Saitou M
  • Journal Title: Cell Volume: 137 Pages: 571-584
  • Peer Reviewed
• [Journal Article] The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice (2009)
  • Author(s): Van Thuan N, Bui HT, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T
  • Journal Title: Reproduction Volume: 138 Pages: 309-317
  • Peer Reviewed
• [Journal Article] Assessment of chromosomal integrity using a novel live-cell imaging technique in mouse embryos produced by intracytoplasmic sperm injection (2009)
  • Author(s): Yamagata K, Suetsugu R, Wakayama T
  • Journal Title: Hum. Reprod Volume: 24 Pages: 2490-2499
  • Peer Reviewed
• [Journal Article] Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos (2009)
  • Author(s): Oda, M. Tanaka, S. Yamazaki, Y. Ohta, H. Iwatani, M. Suzuki, M. Ohgane, J. Hattori, N. Yanagimachi, R. Wakayama, T. Shiota, K
  • Journal Title: Proc Natl Acad Sci U S A Volume: 106 Pages: 16293-16297
  • Peer Reviewed
• [Journal Article] Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI (2009)
  • Author(s): Li et al.
  • Journal Title: Reproduction 137 Pages: 779-792
  • Peer Reviewed
• [Journal Article] Long-term, Six-dimensional Live-cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-term Development (2009)
  • Author(s): Yamagata et al.
  • Journal Title: J Reprod Dev 55 Pages: 343-350
  • Peer Reviewed
• [Journal Article] Effect of Vanadate on the Chromatin Configuration in Pig G V-oocytes (2009)
  • Author(s): Bui et al.
  • Journal Title: J Reprod Dev 55 Pages: 571-584
  • Peer Reviewed
• [Journal Article] The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice (2009)
  • Author(s): Van Thuan et al.
  • Journal Title: Reproduction 138 Pages: 309-317
  • Peer Reviewed
• [Journal Article] Assessment of chromosomal integrity using a novel live-cell imaging technique in mouse embryos produced by intracytoplasmic sperm injection (2009)
  • Author(s): Yamagata et al.
  • Journal Title: Hum.Reprod 24 Pages: 2490-2499
  • Peer Reviewed
• [Journal Article] Detrimental Effects of Microgravity on Mouse Preimplantation Development in Vitro (2009)
  • Author(s): Wakayama et al.
  • Journal Title: PloS ONE 4
  • NAID: 120006830382
  • Peer Reviewed
• [Journal Article] Male Germline and Embryonic Stem Cell Lines from NOD Mice : Efficient Derivation of GS Cells from a Nonpermissive Strain for ES Cell Derivation (2009)
  • Author(s): Ohta et al.
  • Journal Title: Biol.Reprod 81 Pages: 1147-1153
  • Peer Reviewed
• [Journal Article] Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos (2009)
  • Author(s): Oda et al.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 16293-16297
  • Peer Reviewed
• [Journal Article] Enhanced apoptosis during early neuronal defferntiation in mouse ES cells with autosomal imbalance. (2009)
  • Author(s): Kai Y, et al.
  • Journal Title: Cell Res. 19 Pages: 247-258
  • Peer Reviewed
• [Journal Article] Effect of volume of oocyte cytoplasm on embryo development after parthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer (2008)
  • Author(s): Wakayama S, Kishigami S, Thuan NV, Ohta H, Hikichi T, Mizutani E, Bui HT, Miyake M, and Wakayama T
  • Journal Title: Zygote Volume: 16 Pages: 211-222
  • Peer Reviewed
• [Journal Article] Nuclear Transfer Alters the DNA Methylation Status of Specific Genes in Fertilized And Parthenogenetically Activated Mouse Embryonic Stem Cells (2008)
  • Author(s): Hikichi T, Kohda T, Wakayama S, Ishino F, and Wakayama T
  • Journal Title: Stem Cells Volume: 26 Pages: 783-788
  • Peer Reviewed
• [Journal Article] The Birth of Mice from Testicular Spermatozoa Retrieved from Frozen Testicular Sections (2008)
  • Author(s): Ohta H, Sakaide Y, and Wakayama T
  • Journal Title: Biol Reprod Volume: 78 Pages: 807-811
  • Peer Reviewed
• [Journal Article] Therapeutic cloning in individual parkinsonian mice (2008)
  • Author(s): Tabar V, Tomishima M, Panagiotakos G, Wakayama S, Menon J, Chan B, Mizutani E, Al-Shamy G, Ohta H, Wakayama T, and Studer L
  • Journal Title: Nat. Med Volume: 14 Pages: 379-381
  • Peer Reviewed
• [Journal Article] X-chromosome inactivation in nuclear transfer ES cells (2008)
  • Author(s): Shibata S, Yokota T, and Wakayama T
  • Journal Title: Cytogenetic and Genome Research Volume: 121 Pages: 96-101
  • NAID: 120001130203
  • Peer Reviewed
• [Journal Article] Genome-Wide Analysis of Abnormal H3K9 Acetylation in Cloned Mice (2008)
  • Author(s): Suzuki T, Kondo S, and Wakayama T, Cizdziel PE, and Hayashizaki Y
  • Journal Title: PLoS One Volume: 3
  • Peer Reviewed
• [Journal Article] Propagation of senescent mice using nuclear transfer embryonic stem cell lines (2008)
  • Author(s): Mizutani E, Ono T, Li Chong, Maki-Suetsugu R, Wakayama T
  • Journal Title: Genesis Volume: 46 Pages: 478-483
  • Peer Reviewed
• [Journal Article] Nuclear transfer preserves the nuclear genome of freeze-dried mouse cells (2008)
  • Author(s): Ono T., Mizutani E., Li C., Wakayama T
  • Journal Title: J. Reprod Dev Volume: 54 Pages: 486-491
  • NAID: 10024940504
  • Peer Reviewed
• [Journal Article] The cytoplasm of mouse germinal vesicle (GV) stage oocytes can enhance somatic cell nuclear reprogramming (2008)
  • Author(s): Bui HT., Wakayama S., Kishigami S., Thuan NV. Wakayama T
  • Journal Title: Development Volume: 135 Pages: 3935-3945
  • Peer Reviewed
• [Journal Article] Production of healthy cloned mice from bodies frozen at -20℃ for 16 years, (2008)
  • Author(s): Wakayama S, Ohta H, Hikichi T, Mizutani E, Iwaki T, Kanagawa O, Wakayama T
  • Journal Title: Proc Natl Acad Sci U S A Volume: 105 Pages: 17318-17322
  • NAID: 10024965073
  • Peer Reviewed
• [Journal Article] An epigenetic aberration increased in intergenic regions of cloned mice (2008)
  • Author(s): Nishida H, Kondo S, Suzuki T, Tsujimura Y, Komatsu S, Wakayama T, Y Hayashizaki
  • Journal Title: Mammalian Genome Volume: 19 Pages: 667-674
  • Peer Reviewed
• [Journal Article] The cytoplasm of mouse germinal vesicle (GV) stage oocytes can enhance somatic cell nuclear reprogramming (2008)
  • Author(s): Bui HT., Wakayama S., Kishigami S., Thuan NV.Wakayama T.
  • Journal Title: Development 135 Pages: 3935-3945
  • Peer Reviewed
• [Journal Article] Production of healthy cloned mice from bodies frozen at -20^0C for 16 years (2008)
  • Author(s): Wakayama S, Ohta H, Hikichi T, Mizutani E, Iwaki T, Kanagawa O, Wakayama T
  • Journal Title: Proc Natl Acad Sci U S A. 105 Pages: 17318-17322
  • Peer Reviewed
• [Journal Article] The Birth of Mice from Testicular Speumatozoa Retrieved from Frozen Testicular Sections. (2008)
  • Author(s): Ohta H, et al.
  • Journal Title: Biol Reprod 78 Pages: 807-811
  • Peer Reviewed
• [Journal Article] Nuclear Transfer Alters the DNA Methylation Status of Specific Genes in Fertilized And Parthenogenetically Activated Mouse Embryonic Stem Cells. (2008)
  • Author(s): Hikichi T, et al
  • Journal Title: Stem Cells 26 Pages: 783-788
  • Peer Reviewed
• [Journal Article] Effect of volume of oocyte cytoplasm on embryo development after prthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer. (2008)
  • Author(s): Wakayama S, et al.
  • Journal Title: Zygote 16 Pages: 221-222
  • Peer Reviewed
• [Journal Article] Therapeutic cloning in individual parkinsonian mice. (2008)
  • Author(s): Tabar V, et al.
  • Journal Title: Nat.Med 14 Pages: 379-381
  • Peer Reviewed
• [Journal Article] Genome-Wide Analysis of Abnormal H3K9 Acetylation in Cloned Mice (2008)
  • Author(s): Suzuki T, et al.
  • Journal Title: PLoS One 3
  • Peer Reviewed
• [Journal Article] Long-Term Preservation of Mouse Spermatozoa as Frozen Testicular Sctions (2008)
  • Author(s): Ohta H, et al
  • Journal Title: J.Reprod.Dev 54 Pages: 295-298
  • Peer Reviewed
• [Journal Article] propagation of senescent mice using nucrear transfer embryonicstem cell lines (2008)
  • Author(s): Mizutani E, et al.
  • Journal Title: Genesis 46 Pages: 478-483
  • Peer Reviewed
• [Journal Article] Generation of mice derived from embryonic stem cells using blastocysts of different developmental ages. (2008)
  • Author(s): Ohta H, et al.
  • Journal Title: Reproduction 136 Pages: 581-587
  • Peer Reviewed
• [Journal Article] Increasing the cell number of host tetraploid embryos canimprove the production of mice derived from embryonic stem cells (2008)
  • Author(s): Ohta H, et al.
  • Journal Title: Biol.Reprod 79 Pages: 486-492
  • Peer Reviewed
• [Journal Article] Nuclear Transfer Preserves the Nuclear Genome of Freeze-Dried Mouse Cells. (2008)
  • Author(s): Ono T, et al.
  • Journal Title: J.Reprod.Dev 54 Pages: 486-491
  • Peer Reviewed
• [Journal Article] Production of healthy cloned mice from bodies frozen at -20 degrees C for 16years. (2008)
  • Author(s): Wakayama S, et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA 10 Pages: 17318-17322
  • Peer Reviewed
• [Journal Article] The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming. (2008)
  • Author(s): Bui HT, et al.
  • Journal Title: Development 135 Pages: 3935-3945
  • Peer Reviewed
• [Journal Article] Functional Analysis of Male MOuse Haploid Germ Cells of Various Differentiation Stages : Early and Late Round Spermatids Are Functionally Equivalent in Producing Progeny (2008)
  • Author(s): Ohta H, et al.
  • Journal Title: Biol.Reprod. (in press)
  • Peer Reviewed
• [Journal Article] Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI (2008)
  • Author(s): Ohta H, et al.
  • Journal Title: Hum.Reprod. (in press)
  • Peer Reviewed
• [Presentation] マンモスは再生出来るか? (2012)
  • Author(s): 若山照彦
  • Organizer: 日本生殖免疫学会
  • Place of Presentation: 大阪府 高槻市
  • Year and Date: 2012-12-08
• [Presentation] クローン動物の低出産率の原因は何か。 (2012)
  • Author(s): 若山照彦
  • Organizer: 特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」
  • Place of Presentation: 京都府 京都市
  • Year and Date: 2012-11-21
• [Presentation] クローン技術の現状と絶滅動物の復活 (2012)
  • Author(s): 若山照彦
  • Organizer: 脳心血管抗加齢研究会
  • Place of Presentation: 大阪府 大阪市
  • Year and Date: 2012-11-17
• [Presentation] 自分の土俵-餅は餅屋で世界一を目指せ- (2012)
  • Author(s): 若山照彦
  • Organizer: 分子生物学会若手の会
  • Place of Presentation: 山梨県 石和市
  • Year and Date: 2012-04-25
• [Presentation] Development of novel cloning techniques (2012)
  • Author(s): Teruhiko Wakayama
  • Organizer: The 8th Annual Meeting of Asian Reproductive Biotech nology Society
  • Place of Presentation: マニラ、フィリピン
• [Presentation] 哺乳類の宇宙繁殖-人類は宇宙で子孫を作れるか?- (2011)
  • Author(s): 若山照彦
  • Organizer: きぼう利用シンポジウム「きぼう」に夢を乗せて理化学研究所
  • Place of Presentation: 和光、埼玉
  • Year and Date: 2011-11-30
• [Presentation] クローン技術～マンモスが蘇り、再生医療が進歩する!?～ (2011)
  • Author(s): 若山照彦
  • Organizer: 関西広域バイオメディカルクラスター
  • Place of Presentation: 神戸、兵庫
  • Year and Date: 2011-03-21
• [Presentation] 生殖工学を用いた新たな動物繁殖技術 (2011)
  • Author(s): 若山照彦
  • Organizer: 第4回IN VIVOシンポジウム
  • Place of Presentation: 学士会館 東京都
  • Year and Date: 2011-02-23
• [Presentation] Development of novel cloning and ART methods for animal production and species preservation (2011)
  • Author(s): Wakayama, Teruhiko
  • Organizer: The 2nd World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
• [Presentation] Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy (2011)
  • Author(s): Wakayama, Teruhiko
  • Organizer: The 8th Annual Meeting of Asian Reproductive Biotechnology Society
  • Place of Presentation: Guilin, China
• [Presentation] マウス初期胚の発生における微小重力の影響 (2011)
  • Author(s): 若山清香、河原裕美、李羽中、山縣一夫、弓削類、若山照彦
  • Organizer: 宇宙利用シンポジウム(第27回)プログラム
  • Place of Presentation: 宇宙航空研究開発機構・相模原キャンパス 相模原市
• [Presentation] Nuclear transfer as a new tool for preserving mouse strain. (2010)
  • Author(s): Wakayama T.
  • Organizer: 9th Transgenic Technology meeting
  • Place of Presentation: Berlin Germany
• [Presentation] Nuclear transfer as a new tool for study of reproduction and genome preservation.Epigenetics, chromatin & Transcription (2010)
  • Author(s): Wakayama T.
  • Organizer: Cold Spring Harbor Conferences Asia
  • Place of Presentation: Suzhou, China
• [Presentation] Mammalian reproduction in space (2010)
  • Author(s): T.Wakayama
  • Organizer: Symposium on Scientific Utilization of JEM Kibo on International Space Station
  • Place of Presentation: RIKEN Wako和光市
• [Presentation] マンモスが復活する日 (2010)
  • Author(s): 若山照彦
  • Organizer: 第59回 日本医学検査学会
  • Place of Presentation: 神戸国際会議場 神戸市
• [Presentation] Nuclear transfer as a new tool for study of reproduction and genome preservation. (2010)
  • Author(s): T.Wakayama
  • Organizer: Cold Spring Harbor ASIA Conference"Epigenetics, Chromatin & Transcription"
  • Place of Presentation: Suzhou Dushu Lake Conference Center, Beijing, China
• [Presentation] Production of normal mice by ICSI using long-term preserved sperm without freezing. (2010)
  • Author(s): Chong Li, Eiji Mizutani, Tetsuo Ono, Wakayama T.
  • Organizer: 11^<th> International Symposium on SPERMATOLOGY OKINAWA 2010
  • Place of Presentation: Okinawa Convention Center那覇市
• [Presentation] Production of cloned mice from not live cell nuclei (2010)
  • Author(s): Teruhiko Wakayama
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: 京都国際会議場 京都市
• [Presentation] Mammalian reproduction in space (2010)
  • Author(s): Teruhiko WAKAYAMA
  • Organizer: Agenda of the 7^<th> Korea-Japan Joint Seminar on Space Environment Utilization Research
  • Place of Presentation: 東京大学 フォーレスト本郷東京都
• [Presentation] How to Improve the Nuclear Reprogramming Potential and its Limitations (2010)
  • Author(s): Teruhiko WAKAYAMA
  • Organizer: 7^<th> Annual Conference of Asian Reproductive Biology Society2010
  • Place of Presentation: Equatorial Hotel, Kuala Lumpur, Malaysia
• [Presentation] Nuclear transfer ES cells as a new tool for basic biology. (2009)
  • Author(s): Wakayama T.
  • Organizer: The sixth Annual Meeting of Asian Reproductive Biotechnology Society.
  • Place of Presentation: Siem Reap City, Cambodia
• [Presentation] Nuclear transfer ES cells as a new tool for basic biology (2009)
  • Author(s): Wakayama T.
  • Organizer: The sixth fifth Annual Meeting of Asian Reproductive Biotechnology Society
  • Place of Presentation: Siem Reap City, Cambodia
• [Presentation] Improvement of efficiency of mouse cloning and ES cell establishment. (2008)
  • Author(s): Wakayama T.
  • Organizer: 12th ADNAT convention.The Biology of Embryonic and Adult Stem Cells, Centre for Cellular and Molecular Biology
  • Place of Presentation: Hyderabad, INDIA
• [Presentation] Production of healthy cloned mice from frozen dead bodies. (2008)
  • Author(s): Wakayama T.
  • Organizer: The fifth Annual Meeting of Asian Reproductive Biotechnology Society
  • Place of Presentation: Horizon Hotel Knming, China。
• [Book] Nuclear transfer in the mouse oocyte In "Mammalian Oocyte Regulation" Ed. by Hayden Homer (Humana press, USA) (2013)
  • Author(s): Mizutani E. Ogura A. and Wakayama T
  • Publisher: Methods in Molecular Biology
• [Book] Cloned mice from Adults Stem Cells. In "Advanced in Molecular Biology and Medicine: Stem cells (2013)
  • Author(s): Obokata, H., Wakayama T
  • Publisher: From Biology to Therapy" Vol.1 (Ed. Robert A. Meyers: Wiley-Blackwell Press)
• [Book] Cloned mice from embryonic stem cells. In "Advanced in Molecular Biology and Medicine: Stem cells (2013)
  • Author(s): Li C., Wakayama S. and Wakayama T
  • Publisher: From Biology to Therapy" Vol.1 (Ed. Robert A. Meyers: Wiley-Blackwell Press)
• [Book] Mammalian Oocyte Regulation (Mizutani et al., Nuclear transfer in the mouse oocyte) (2013)
  • Author(s): Ed. by Hayden Homer
  • Publisher: Humana press
• [Book] Encyclopedia of molecular cell biology and molecular medicine : Stem cells" 3rd edition. (Obokata et al., Cloned mice from adults stem cells) (2013)
  • Author(s): Ed. by Robert A. Meyers
  • Publisher: Wiley press
• [Book] Encyclopedia of molecular cell biology and molecular medicine : Stem cells" 3rd edition. (Li et al., Cloned mice from embryonic stem cells) (2012)
  • Author(s): Ed. by Robert A. Meyers
  • Publisher: Wiley press
• [Book] 体細胞核移植クローン 卵子学(森崇英総編集) (2011)
  • Author(s): 若山清香、若山照彦
  • Publisher: 京都大学学術出版会
• [Book] 卵子学 (2011)
  • Author(s): 若山清香、若山照彦
  • Publisher: 京都大学学術出版会
• [Book] Nuclear transfer ES cells as a new tool for basic biology In: Stem Cells & Regenerative Medicine: From Molecular Embryologyto Tissue Engineering. (ed. Krishnarao Appasani Raghu K. Appasani) (2010)
  • Author(s): Wakayama S. Mizutani E. and Wakayama T
  • Publisher: Humana Press, USA
• [Book] Mouse cloning by nuclear transfer. In: Advanced protocols for animal transgenesis. (ed. Shirley Pease, Thomas L. Saunders) (2010)
  • Author(s): Wakayama S. Thuan NV. and Wakayama T
  • Publisher: Springer New York
• [Book] From Molecular Embryologyto Tissue Engineering(ed.Krishnarao Appasani Raghu K.Appasani)(USA.Stem Cells & Regenerative Medicine) (2010)
  • Author(s): Wakayama S.Mizutani E., Wakayama T.
  • Publisher: Humana Press
• [Book] Nuclear transfer ES cells as a new tool for basic biology In : Stem Cells & Regenerative Medicine : From Molecular Embryologyto Tissue Engineering. (2010)
  • Author(s): Wakayama S., Mizutani E., Wakayama T
  • Publisher: (ed.Krishnarao Appasani RaghuK.Appasani) Humana Press, USA.
• [Book] クローンマンモスへの道-クローン技術最前線の技術における発生・再生医療技術を探る(単行本) (2009)
  • Author(s): 若山照彦
  • Publisher: アドスリー社 東京
• [Book] Generation of mice from embryonic stem cells using tetraploid embryos as hosts. In: Methods in Bioengineering. (ed. Biju Parekkadan and Martin L. Yarmush) (2009)
  • Author(s): Ohta H. and Wakayama T
  • Publisher: Artech House. London
• [Book] Somatic cell nuclear transfer in the mouse. In: Microinjection: Methods and Applications (ed. David J. Carroll) (2009)
  • Author(s): Kishigami S, and Wakayama T
  • Publisher: (Humana Press, USA), Methods in Molecular Biology
• [Book] Cloning of ES cells and mice by nuclear transfer. In: Gene Knockout Protocols ed. Ralf Kuehn, Wolfgang Wurst (Humana Press, USA) (2009)
  • Author(s): Wakayama S, Kishigami S, Wakayama T
  • Publisher: Methods in Molecular Biology
• [Book] Methods in Molecular Biology(ed.Ralf Kuehn, Wolfgang Wurst)(USA.Gene Knockout Protocols)530 (2009)
  • Author(s): Wakayama S, Kishigami S, Wakayama T.
  • Publisher: Humana Press
• [Book] マンモスへの道-クローン技術最前線の技術 における発生・再生医療技術を探る(クローン) (2009)
  • Author(s): 若山照彦
  • Total Pages: 80
  • Publisher: アドスリー社 東京
• [Book] Establishment of individual-specific ES cells from adult somatic cells by nuclear transfer. In: Stem Cell Applications in Diseases (ed. Mikkel L. Sorensen) (2008)
  • Author(s): Wakayama S, and Wakayama T
  • Publisher: Nova Science Publishers, Inc. New York USA
• [Book] Epigenetic remodeling and developmental potentials after intracytoplasmic injection of spermatozoa and spermatids. In: Genetic and Epigenetic Control of Mammalian Germ Cell Development and Function (ed. Masami Nozaki) (2008)
  • Author(s): Kishigami S, Ohta H, and Wakayama T
  • Publisher: Research Signpost. Kerala, India
• [Book] Inc.New York USA, Stem Cell Applications in Diseases(ed.Mikkel L.Sorensen) (2008)
  • Author(s): Wakayama S, Wakayama T.
  • Publisher: Nova Science Publishers
• [Book] クローンマンモスへの道-クローン技術最前線の技術における発生・再生医療技術を探る (2008)
  • Author(s): 若山照彦
  • Total Pages: 77
  • Publisher: アドスリー社
• [Remarks]
  • URL: http://www.bt.yamanashi.ac.jp/modules/kenkyu/index.php?cat_id=5
• [Remarks]
  • URL: http://www.cdb.riken.jp/jp/04_news/articles/13/13010_recloning.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2012/120125/index.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2012/120209/index.html
• [Remarks] ホームページ
  • URL: http://www.cdb.riken.go.jp/grp/
• [Remarks] (所属機関HP)
  • URL: http://www.cdb.riken.jp/jp/02_research/0202_creative07.html
• [Remarks] (研究室HP)
  • URL: http://www.cdb.riken.jp/grp/
• [Remarks]
  • URL: http://www.riken.jp/r-world/info/release/press/2009/090825/index.html
• [Remarks] (理研プレスリリース)
  • URL: http://www.riken.jp/r-world/info/release/press/2008/081104/index.html
• [Remarks] (CDB化学ニュース)
  • URL: http://www.cdb.riken.jp/04_news/articles/081104_frozen.html
• [Patent(Industrial Property Rights)] 顕微鏡および光透過ユニット (2011)
  • Inventor(s): 山縣一夫、若山照彦、今井雄一郎、田村恵裕
  • Industrial Property Rights Holder: 理化学研究所
  • Industrial Property Number: 2011-286996
  • Filing Date: 2011-12-27
• [Patent(Industrial Property Rights)] 動物組織からクローン動物ならびにntES 細胞を作成するための新規方法 (2009)
  • Inventor(s): 若山清香, 若山照彦
  • Industrial Property Rights Holder: 若山清香, 若山照彦
  • Industrial Property Number: 2008-123133
  • Filing Date: 2009-11-19
• [Patent(Industrial Property Rights)] 動物組織からクローン動物ならびにntES細胞を作成するための新規方法 (2008)
  • Inventor(s): 若山照彦、若山清香
  • Industrial Property Rights Holder: 理化学研究所
  • Industrial Property Number: 2008-123133
  • Filing Date: 2008-05-09