| Budget Amount *help |
¥57,330,000 (Direct Cost: ¥44,100,000、Indirect Cost: ¥13,230,000)
Fiscal Year 2012: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2011: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2010: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2009: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2008: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Research Abstract |
Carbohydrate-protein interactions are not strong but they are very important in terms of specific recognition in vital functions. Although it is very easy for thick polyvalent layers to interact with target molecules, the thickness, bulkiness, and heterogeneity of the reaction layers pose serious problems in terms of quantitative analysis and realizing rapid reactions. It will be a challenge to form a thin layer with a polyvalent recognition ability. We investigated the processing of a nanostructured recognition site for sensing lectin, Concanavalin A (Con A), by using self-assembled monolayers (SAMs) consisting of artificial carbohydrate and OH terminated filling molecules formed on a gold substrate. We prepared carbohydrate ligands for recognition of lectins/galectins and tri (ethylene glycol) terminated short alkanethiol molecules for constructing ultra thin protein resistant monolayers on a gold surface. Electrochemical active molecules were combined with carbohydrate terminated molecules, and the mixed monolayer was adopted to detect weak interaction between the carbohydrate and lectins much easily.
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