Elucidation and application of sequence-specific phenomena of DNA-grafted nanoparticles

• Project Area: Molecular Soft-Interface Science
• Project/Area Number: 20106010
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MAEDA Mizuo 独立行政法人理化学研究所, 前田バイオ工学研究室, 主任研究員 (10165657)
• Co-Investigator(Kenkyū-buntansha): TAKARADA Tohru 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (30336010) ; FUJITA Masahiro 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (50342845)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥83,850,000 (Direct Cost: ¥64,500,000、Indirect Cost: ¥19,350,000); Fiscal Year 2012: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000); Fiscal Year 2011: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2010: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2009: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: ソフトインターフェース / 核酸分子 / コンジュゲート材料 / ナノ粒子 / ポリ(N-イソプロピルアクリルアミド) / 生体機能材料 / 遺伝子診断 / 界面科学 / 分子認識

Research Abstract

Nanoparticles densely grafted with double-stranded DNA having a terminal single-base mismatch between the DNA layer and the disperse medium exhibit significantly high colloidal stability compared to those with fully matched DNA. Measurements of small angle X-ray scattering patterns at SPring-8 and force curves by atomic force microscopy revealed that this unique property stems from the fact that steric repulsive force induced by micro-Brownian motion of the terminal bases surpasses van der Waals attractive force between the hydrophobic cores. The elucidation of the mechanism led us to rationally design the surface structure of nanoparticles for the reliable gene mutation assay and the colloidal logic circuits.

Research Products

• [Journal Article] Self-association of zwitterionic polymer-lipid conjugates in water as examined by scattering measurements. (2013)
  • Author(s): Murou, M.; Kitano, H.; Fujita, M.; Maeda, M.; Saruwatari, Y.
  • Journal Title: J. Colloid Interface Sci. Volume: 390 Issue: 1 Pages: 47-53
  • DOI: 10.1016/j.jcis.2012.09.035
  • Peer Reviewed
• [Journal Article] Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol–disulfide exchange (2013)
  • Author(s): Aya Mizutani Akimoto
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 103 Pages: 360-365
  • DOI: 10.1016/j.colsurfb.2012.10.031
  • Peer Reviewed
• [Journal Article] Self-association of zwitterionic polymer-lipid conjugates in water as examined by scattering measurements (2012)
  • Author(s): Masanobu Murou, Hiromi Kitano, Masahiro Fujita, Mizuo Maeda, and Yoshiyuki Saruwatari
  • Journal Title: J. Colloid Interface Sci. Volume: 390 Pages: 47-53
  • Peer Reviewed
• [Journal Article] Thermoresponsive micellization and micellar stability of poly(N-isopropylacrylamide)-b-DNA diblock and miktoarm star polymers (2012)
  • Author(s): Pengju Pan, Masahiro Fujita, Wei-Yang Ooi, Kumar Sudesh, Tohru Takarada, Atsushi Goto, and Mizuo Maeda
  • Journal Title: Langmuir Volume: 28 Pages: 14347-14356
  • Peer Reviewed
• [Journal Article] Structural characterization of nanoparticles from thermoresponsive poly(N-isopropylacrylamide)-DNA conjugate (2012)
  • Author(s): Wei-Yang Ooi, Masahiro Fujita, Pengju Pan, Hui-Ying Tang, Kumar Sudesh, Kazuki Ito, Naoki Kanayama, Tohru Takarada, and Mizuo Maeda
  • Journal Title: J. Colloid Interface Sci. Volume: 374 Pages: 315-320
  • Peer Reviewed
• [Journal Article] Structural study on gold nanoparticle functionalized with DNA and its non-cross-linking aggregation (2012)
  • Author(s): Masahiro Fujita, Yoshizumi Katafuchi, Kazuki Ito, Naoki Kanayama, Tohru Takarada, and Mizuo Maeda
  • Journal Title: J. Colloid Interface Sci. Volume: 368 Pages: 629-635
  • Peer Reviewed
• [Journal Article] DNAがつくるソフト界面の特異な性質 (2012)
  • Author(s): 前田瑞夫
  • Journal Title: 膜 Volume: 37 Pages: 183-188
  • NAID: 10030986195
• [Journal Article] Structural study on gold nanoparticle functionalized with DNA and its non-cross-linking aggregation (2012)
  • Author(s): M.Fujita, Y.Katafuchi, K.Ito, N.Kanayama, T.Takarada, M.Maeda
  • Journal Title: J.Colloid Int.Sci. Volume: 368 Issue: 1 Pages: 629-635
  • DOI: 10.1016/j.jcis.2011.11.021
  • Peer Reviewed
• [Journal Article] Rapid naked-eye detection of mercury ion based on non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles (2011)
  • Author(s): Naoki Kanayama, Tohru Takarada, and Mizuo Maeda
  • Journal Title: Chem. Commun. Volume: 47 Pages: 2077-2079
  • Peer Reviewed
• [Journal Article] RAFT-generated poly(N-isopropylacrylamide)-DNA block copolymers for temperature-responsive formation of polymer micelles (2011)
  • Author(s): Kyosuke Isoda, Naoki Kanayama, Daisuke Miyamoto, Tohru Takarada, and Mizuo Maeda
  • Journal Title: React. Funct. Polym. Volume: 71 Pages: 367-370
  • Peer Reviewed
• [Journal Article] DNA-functionalized thermoresponsive bioconjugates synthesized via ATRP and click chemistry (2011)
  • Author(s): Pengju Pan, Masahiro Fujita, Wei-Yang Ooi, Kumar Sudesh, Tohru Takarada, Atsushi Goto, and Mizuo Maeda
  • Journal Title: Polymer Volume: 52 Pages: 895-900
  • Peer Reviewed
• [Journal Article] 生体高分子ソフトインターフェースの科学 (2011)
  • Author(s): 前田瑞夫
  • Journal Title: プラズマ・核融合学会誌 Volume: 87 Pages: 691-695
  • NAID: 110008750648
• [Journal Article] Rapid Naked-eye Detection of Mercury Ion Based on Non-cross linking Aggregation of Double-stranded DNA-carrying Gold Nanoparticles (2011)
  • Author(s): N. Kanayama, T. Takarada, M. Maeda
  • Journal Title: Chem. Commun 47 Pages: 2077-2079
  • Peer Reviewed
• [Journal Article] RAFT-generated poly(N-isopropylacrylamide)-DNA block copolymers for temperature-responsive formation of polymer micelles (2011)
  • Author(s): K. Isoda, N. Kanayama, D. Miyamoto, T. Takarada, M. Maeda
  • Journal Title: Reactive & Functional Polymers 71 Pages: 367-371
  • Peer Reviewed
• [Journal Article] DNA-Functionalized Ther moresponsive Bioconjugates Synthesized via ATRP and Click Chemistry (2011)
  • Author(s): P. Pan, M. Fujita, W.Y. Ooi, K. Sudesh, T. Takarada, A. Goto, M. Maeda
  • Journal Title: Polymer 52 Pages: 895-900
  • Peer Reviewed
• [Journal Article] Rapid Naked-eye Detection of Mercury Ion Based on Non-crosslinking Aggregation of Double-stranded DNA-carrying Gold Nanoparticles (2011)
  • Author(s): N.Kanayama, T.Takarada, M.Maeda
  • Journal Title: Chem.Commun. Volume: 47 Pages: 2077-2079
  • Peer Reviewed
• [Journal Article] RAFT-generated poly (N-isopropylacrylamide)-DNA block copolymers for temperature-responsive formation of polymer micelles (2011)
  • Author(s): K.Isoda, N.Kanayama, D.Miyamoto, T.Takarada, M.Maeda
  • Journal Title: Reactive & Functional Polymers Volume: 71 Pages: 367-371
  • Peer Reviewed
• [Journal Article] DNA-Functionalized Thermoresponsive Bioconjugates Synthesized via ATRP and Click Chemistry (2011)
  • Author(s): P.Pan, M.Fujita, W.Y.Ooi, K.Sudesh, T.Takarada, A.Goto, M.Maeda
  • Journal Title: Polymer Volume: 52 Pages: 895-900
  • Peer Reviewed
• [Journal Article] A facile method towards cyclic assembly of gold nanoparticles using DNA template alone (2010)
  • Author(s): Takahito Ohshiro, Tamotsu Zako, Ryoko Watanabe-Tamaki, Takuo Tanaka, and Mizuo Maeda
  • Journal Title: Chem. Commun. Volume: 46 Pages: 6132-6134
  • Peer Reviewed
• [Journal Article] Single-molecule imaging of DNA duplexes immobilized on surfaces with a scanning tunneling microscope (2010)
  • Author(s): Takahito Ohshiro and Mizuo Maeda
  • Journal Title: Chem. Commun. Volume: 46 Pages: 2581-2583
  • Peer Reviewed
• [Journal Article] A Facile Method Towards Cyclic Assembly of Gold Nanoparticles Using DNA Template Alone (2010)
  • Author(s): T. Ohshiro, T. Zako, R. Wanatabe-Tamaki, T. Tanaka, M. Maeda
  • Journal Title: Chem. Commun. 46 Pages: 6132-6134
  • Peer Reviewed
• [Journal Article] Single-molecule Imaging of DNA Duplexes Immobilized on Surfaces with a Scanning Tunneling Microscope (2010)
  • Author(s): T. Ohshiro, M. Maeda
  • Journal Title: Chem. Commun 46 Pages: 2581-2583
  • Peer Reviewed
• [Journal Article] A Facile Method Towards Cyclic Assembly of Gold Nanoparticles Using DNA Template Alone (2010)
  • Author(s): T.Ohshiro, T.Zako, R.Wanatabe-Tamaki, T.Tanaka, M.Maeda
  • Journal Title: Chem.Commun. Volume: 46 Pages: 6132-6134
  • Peer Reviewed
• [Journal Article] Degradation and Adsorption Characteristics of PHB Depolymerase as Revealed by Kinetics for the Mutant Enzymes with Amino Acid Substitution in Substrate-binding Domain (2010)
  • Author(s): T.Hiraishi, N.Komiya, N.Matsumoto, H.Abe, M.Fujita, M.Maeda
  • Journal Title: Biomacromolecules Volume: 11 Pages: 113-119
  • Peer Reviewed
• [Journal Article] Single-molecule Imaging of DNA Duplexes Immobilized on Surfaces with a Scanning Tunneling Microscope (2010)
  • Author(s): T.Ohshiro, M.Maeda
  • Journal Title: Chem.Commun. Volume: 46 Pages: 2581-2583
  • Peer Reviewed
• [Journal Article] DNAコンジュゲート材料によるバイオ計測法の開発 (2010)
  • Author(s): 金山直樹、前田瑞夫
  • Journal Title: ファルマシア Volume: 46 Pages: 317-322
• [Journal Article] ソフトインターフェースの分子科学 (2010)
  • Author(s): 前田瑞夫
  • Journal Title: 応用物理 Volume: 79 Pages: 219-224
  • NAID: 10026199482
• [Journal Article] ソフトインターフェース-高分子界面の新しい機能- (2009)
  • Author(s): 藤田雅弘、前田瑞夫
  • Journal Title: 高分子 58 Pages: 483-488
  • NAID: 10025093634
• [Journal Article] Simple and rapid colorimetric detection of cofactors of aptazymes using noncrosslinking gold nanoparticle aggregation (2008)
  • Author(s): Atsushi Ogawa and Mizuo Maeda
  • Journal Title: Bioorg. Med. Chem. Lett., Volume: 18 Pages: 6517-6520
  • Peer Reviewed
• [Journal Article] Structural characterization of nanoparticles from thermoresponsive poly (N-isopropylacrylamide)-DNA conjugate
  • Author(s): W.-Y.Ooi, M.Fujita, P.Pan, H.-Y.Tang, K.Sudesh, K.Ito, N.Kanayama, T.Takarada, M.Maeda
  • Journal Title: J.Colloid Int.Sci. Volume: (掲載確定)
  • Peer Reviewed
• [Presentation] DNA-carrying nanoparticles: synthesis, unique properties, characterization and applications (2013)
  • Author(s): Mizuo Maeda
  • Organizer: ICBS2013
  • Place of Presentation: Ibaraki, Japan
  • Year and Date: 2013-03-21
• [Presentation] DNA-carrying nanoparticles: synthesis, unique properties, characterization and applications (2013)
  • Author(s): M. Maeda
  • Organizer: ICBS2013
  • Place of Presentation: Tsukuba, Ibaraki
  • Year and Date: 2013-03-21
  • Invited
• [Presentation] Synthesis and characteristics of DNA/Pol(N-isopropylacrylamide) bioconjugate (2013)
  • Author(s): M. Fujita
  • Organizer: SIMS2013
  • Place of Presentation: Fukuoka
  • Year and Date: 2013-03-14
• [Presentation] DNA二重鎖ブラシ界面間における特異的相互作用の発現と応用 (2013)
  • Author(s): 金山 直樹
  • Organizer: 第15回高分子表面研究用論会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2013-02-01
• [Presentation] PolyNIPAM-DNA conjugate: unique properties and applications (2012)
  • Author(s): Mizuo Maeda
  • Organizer: NIPAM-80
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2012-12-16
• [Presentation] PolyNIPAM-DNA conjugate unique properties and applications (2012)
  • Author(s): M. Maeda
  • Organizer: NIPAM-80
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2012-12-16
  • Invited
• [Presentation] Target-induced stability change of DNA brush-protected gold nanoparticle (2012)
  • Author(s): N. Kanayama
  • Organizer: IPC2012
  • Place of Presentation: Kobe
  • Year and Date: 2012-12-12
• [Presentation] DNA担持ナノ粒子が示す界面現象の特性評価 (2012)
  • Author(s): 藤田 雅弘
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台市
  • Year and Date: 2012-11-26
• [Presentation] DNA修飾ナノ粒子を用いる分析化学 (2012)
  • Author(s): 前田 瑞夫
  • Organizer: 日本分析化学会 第9回茨城地区分析技術交流会
  • Place of Presentation: 茨城県那珂郡東海村
  • Year and Date: 2012-11-22
  • Invited
• [Presentation] Structural Study on Non-cross-linking Aggregation of DNA-functionalized Nanoparticles (2012)
  • Author(s): M. Fujita
  • Organizer: SISYS2012
  • Place of Presentation: Tsukuba, Ibaraki
  • Year and Date: 2012-11-21
• [Presentation] Logical regulation of the dispersion stability of DNA duplex-immobilized gold nanoparticles via metal-mediated base pairing (2012)
  • Author(s): N. Kanayama
  • Organizer: ISNAC2012
  • Place of Presentation: Nagoya
  • Year and Date: 2012-11-15
• [Presentation] DNA複合化ナノ構造体の開発 (2012)
  • Author(s): 金山 直樹
  • Organizer: 神奈川大学 理学研究科 総合理学研究所講演会
  • Place of Presentation: 神奈川県平塚市
  • Year and Date: 2012-11-09
  • Invited
• [Presentation] DNA修飾ナノ粒子を用いる分析化学 (2012)
  • Author(s): 前田 瑞夫
  • Organizer: 第28回日本イオン交換研究発表会
  • Place of Presentation: 東京都目黒区
  • Year and Date: 2012-10-18
  • Invited
• [Presentation] DNA-functionalized nanoparticle biosensors (2012)
  • Author(s): Mizuo Maeda
  • Organizer: 7th Sweden-Japan BioNano Workshop
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2012-10-16
• [Presentation] Simple and rapid colorimetric biosensors based on non-crosslinking aggregation of DNA-immobilized nanoparticles (2012)
  • Author(s): N. Kanayama
  • Organizer: 7th Sweden-Japan BioNano Workshop
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2012-10-16
• [Presentation] 生物模倣とソフト界面 (2012)
  • Author(s): 前田 瑞夫
  • Organizer: 科研費新学術領域「生物規範工学」公開ワークショップ
  • Place of Presentation: 東京都台東区
  • Year and Date: 2012-10-02
  • Invited
• [Presentation] Non-crosslinking aggregation of DNA-functionalized nanoparticles (2012)
  • Author(s): M. Fujita
  • Organizer: IUMRS-ICEM 2012
  • Place of Presentation: Yokohama
  • Year and Date: 2012-09-26
• [Presentation] Synthesis and Characterization of Poly(N-isopropylacrylamide)-DNA Copolymers (2012)
  • Author(s): H. Hiramine
  • Organizer: IUMRS-ICEM 2012
  • Place of Presentation: Yokohama
  • Year and Date: 2012-09-26
• [Presentation] 第61回高分子討論会 (2012)
  • Author(s): 藤田 雅弘
  • Organizer: DNA担持ナノ粒子の構造物性評価
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-09-20
• [Presentation] DNAブラシ界面の柔軟性に基づくコロイド粒子の分散制御 (2012)
  • Author(s): 金山 直樹
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-09-19
• [Presentation] 新しいセンシング材料としてのDNA担持ナノ粒子 (2012)
  • Author(s): 前田 瑞夫
  • Organizer: 日本分析化学会 第26回新潟地区部会研究発表会
  • Place of Presentation: 新潟市
  • Year and Date: 2012-09-14
  • Invited
• [Presentation] DNA-carrying gold nanoparticles for bioanalytical applications (2012)
  • Author(s): Mizuo Maeda
  • Organizer: GOLD 2012
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2012-09-08
• [Presentation] DNA-carrying gold nanoparticles for bioanalytical applications (2012)
  • Author(s): M. Maeda
  • Organizer: GOLD 2012
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2012-09-08
  • Invited
• [Presentation] Non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles with a thymine-thymine mismatch site through the selective complexation with mercury(II) ion (2012)
  • Author(s): N. Kanayama
  • Organizer: GOLD 2012
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2012-09-06
• [Presentation] ナノポアによる多糖類の１分子解析 (2012)
  • Author(s): 武政 誠
  • Organizer: 第22回バイオ・高分子シンポジウム
  • Place of Presentation: 東京都目黒区
  • Year and Date: 2012-06-26
• [Presentation] 末端ミスマッチ構造により分散安定化したDNA二重鎖担持金ナノ粒子の金属イオン選択的凝集 (2012)
  • Author(s): 金山 直樹
  • Organizer: 第22回バイオ・高分子シンポジウム
  • Place of Presentation: 東京都目黒区
  • Year and Date: 2012-06-26
• [Presentation] Poly(N-isopropylacrylamide)-DNA共重合体の精密合成と特性評価 (2012)
  • Author(s): 平峯 勇人
  • Organizer: 第22回バイオ・高分子シンポジウム
  • Place of Presentation: 東京都目黒区
  • Year and Date: 2012-06-25
• [Presentation] DNA-functionalized nanoparticles for biosensing (2012)
  • Author(s): Mizuo Maeda
  • Organizer: 4th International Conference: Smart Materials, Structures and Systems
  • Place of Presentation: Montecatini Terme, Italy
  • Year and Date: 2012-06-13
• [Presentation] DNA-functionalized nanoparticles for biosensing (2012)
  • Author(s): M. Maeda
  • Organizer: 4th International Conference “Smart Materials, Structures and Systems
  • Place of Presentation: Montecatini Terme, Italy
  • Year and Date: 2012-06-13
  • Invited
• [Presentation] DNA-carrying nanoparticles with bioactive soft interface (2012)
  • Author(s): Mizuo Maeda
  • Organizer: 9th World Biomaterials Congress
  • Place of Presentation: Chengdu, China
  • Year and Date: 2012-06-02
• [Presentation] チミン-チミンミスマッチ末端を有するDNA二重鎖担持金ナノ粒子の水銀イオン選択的凝集現象 (2012)
  • Author(s): 金山 直樹
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-05-31
• [Presentation] ミスマッチ構造により分散安定化したDNA二重鎖担持金ナノ粒子の重金属イオン選択的凝集応答 (2012)
  • Author(s): 金山 直樹
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-05-30
• [Presentation] Synthesis and characterization of poly(N-isopropylacrylamide)-DNA copolymer (2012)
  • Author(s): H. Hiramine
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-05-30
• [Presentation] Mercury(II) ion-specific non-crosslinking aggregation behavior of double-standed DNA-carrying gold nanoparticles with a thymine-thymine mismatch site (2012)
  • Author(s): N. Kanayama
  • Organizer: IACIS2012
  • Place of Presentation: Sendai
  • Year and Date: 2012-05-16
• [Presentation] Synthesis and characterization of poly(N-isopropylacrylamide)-DNA copolymer (2012)
  • Author(s): H. Hiramine
  • Organizer: IACIS2012
  • Place of Presentation: Sendai
  • Year and Date: 2012-05-15
• [Presentation] Structural study on non-crosslinking aggregation of DNA-functionalized gold nanoparticles (2012)
  • Author(s): M. Fujita
  • Organizer: IACIS2012
  • Place of Presentation: Sendai
  • Year and Date: 2012-05-15
• [Presentation] DNA複合体高分子を用いたアフィニティ電気泳動法の開発と遺伝子検査への応用 (2012)
  • Author(s): 前田 瑞夫
  • Organizer: 第62回日本電気泳動学会シンポジウム
  • Place of Presentation: 東京都港区
  • Year and Date: 2012-05-11
  • Invited
• [Presentation] DNAがつくるソフト界面の特異な性質 (2012)
  • Author(s): 前田 瑞夫
  • Organizer: 日本膜学会第34年会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2012-05-09
  • Invited
• [Presentation] Molecular soft-interface science for reliable biosensing (2012)
  • Author(s): Mizuo Maeda
  • Organizer: Bio Tech 2012
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2012-04-27
• [Presentation] Molecular soft-interface science for reliable biosensing (2012)
  • Author(s): M. Maeda
  • Organizer: Bio Tech 2012
  • Place of Presentation: 東京都江東区
  • Year and Date: 2012-04-27
  • Invited
• [Presentation] DNA-functionalized nanoparticles for biosensing (2012)
  • Author(s): Mizuo Maeda
  • Organizer: The 2nd RIKEN-McGill University Scientific Workshop
  • Place of Presentation: Saitama, Japan
  • Year and Date: 2012-04-26
• [Presentation] DNA-functionalized nanoparticles for biosensing (2012)
  • Author(s): M. Maeda
  • Organizer: The 2nd RIKEN-McGill Univ. Scientific Workshop
  • Place of Presentation: Wako, Saitama
  • Year and Date: 2012-04-26
  • Invited
• [Presentation] Biosensor nanoparticles using DNA-based soft interface (2012)
  • Author(s): Mizuo Maeda
  • Organizer: SIMS 2012
  • Place of Presentation: Ibaraki, Japan
  • Year and Date: 2012-03-18
• [Presentation] ソフトインターフェースの分子科学 (2011)
  • Author(s): 前田瑞夫
  • Organizer: 第21回日本MRS学術シンポジウム
  • Place of Presentation: 横浜市開港記念館(横浜市)(招待講演)
  • Year and Date: 2011-12-20
• [Presentation] ナノ粒子を操るバイオ工学 (2011)
  • Author(s): 前田瑞夫
  • Organizer: さきがけ「ナノ製造技術の探索と展開」研究領域研究報告会
  • Place of Presentation: KKRホテル東京(東京都)(招待講演)
  • Year and Date: 2011-12-12
• [Presentation] DNA担持金ナノ粒子の非架橋型凝集の構造解析 (2011)
  • Author(s): 小林奈央, 佐藤香枝, 引間孝明, 藤田雅弘, 前田瑞夫
  • Organizer: 第33回日本バイオマテリアル学会大会
  • Place of Presentation: 京都府総合交流プラザ京都テルサ(京都市)
  • Year and Date: 2011-11-22
• [Presentation] Structural Study On Non-Crosslinking Aggregation Of DNA-Functionalized Gold Nanoparticles (2011)
  • Author(s): M.Fujita, M.Maeda
  • Organizer: PPC12
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2011-11-14
• [Presentation] Structural Study on Thermoresponsive Poly (N-isopropylacrylamide)/DNA Bioconjugate (2011)
  • Author(s): W.-Y.Ooi, P.Pan, K.Sudesh, M.Fujita, M.Maeda
  • Organizer: PPC12
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2011-11-14
• [Presentation] Structural characterization of thermoresponsive PNIPAAm/DNA bioconjugate (2011)
  • Author(s): W.-Y.Ooi, P.Pan, K.Sudesh, T.Hikima, M.Fujita, M.Maeda
  • Organizer: 第60回高分子討会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2011-09-30
• [Presentation] DNA担持金ナノ粒子の非架橋型凝集の構造解析 (2011)
  • Author(s): 小林奈央, 佐藤香枝, 引間孝明, 藤田雅弘, 前田瑞夫
  • Organizer: 第60回高分子討会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2011-09-30
• [Presentation] DNA-based soft interface for biosensing (2011)
  • Author(s): Mizuo Maeda
  • Organizer: ASAM-3
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2011-09-21
• [Presentation] DNA-based soft interface for biosensing (2011)
  • Author(s): M.Maeda
  • Organizer: 第3回アジア先端材料シンポジウム(ASAM-3 2011)
  • Place of Presentation: 九州大学筑紫キャンパス(福岡県春日市)(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] DNAを一成分とするナノ構造体の開発 (2011)
  • Author(s): 金山直樹
  • Organizer: 富山大学固液界面科学研究会
  • Place of Presentation: 富山大学(富山市)(招待講演)
  • Year and Date: 2011-09-10
• [Presentation] ミスマッチ構造により分散安定化したDNA二重鎖担持金ナノ粒子の重金属イオン選択的凝集現象 (2011)
  • Author(s): 金山直樹, 宝田徹, 前田瑞夫
  • Organizer: 第63回コロイドおよび界面化学討論会
  • Place of Presentation: 京都大学吉田キャンパス(京都市)
  • Year and Date: 2011-09-09
• [Presentation] Chemical-, ion-, and bio-sensors using DNA-modified nanoparticles (2011)
  • Author(s): Mizuo Maeda
  • Organizer: Polymers in Medicine and Biology 2011
  • Place of Presentation: California, USA
  • Year and Date: 2011-09-04
• [Presentation] Chemical-, ion-, and bio-sensors using DNA-modified nanoparticles (2011)
  • Author(s): M.Maeda
  • Organizer: Polymers in Medicine and Biology 2011
  • Place of Presentation: Santa Rosa, CA, USA(招待講演)
  • Year and Date: 2011-09-04
• [Presentation] Non-crosslinking aggregation of DNA-functionalized nanoparticles (2011)
  • Author(s): M.Fujita, M.Maeda
  • Organizer: IUCr2011
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2011-08-23
• [Presentation] Nano-structural characterization of PNIPAAm-DNA conjugate colloidal particle (2011)
  • Author(s): W.-Y.Ooi, M.Fujita, P.Pan, K.Sudesh, M.Maeda
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2011-05-27
• [Presentation] Nanoparticle-based sensors using DNA-modified gold colloids (2011)
  • Author(s): Mizuo Maeda
  • Organizer: ICAS 2011
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-05-24
• [Presentation] Nanoparticle-based Sensors Using DNA-modified Gold Colloids (2011)
  • Author(s): M.Maeda
  • Organizer: ICAS2011
  • Place of Presentation: 京都国際会館(京都市)(招待講演)
  • Year and Date: 2011-05-24
• [Presentation] Double-stranded DNA-carrying gold nanoparticles with thymine-thymine mismatch site for facile and rapid detection of mercury ion in water (2011)
  • Author(s): N.Kanayama, T.Takarada, M.Maeda
  • Organizer: ICAS2011
  • Place of Presentation: 京都国際会館(京都市)
  • Year and Date: 2011-05-23
• [Presentation] DNA 担持ナノ粒子の非架橋凝集 (2011)
  • Author(s): 藤田雅弘
  • Organizer: つくばソフトマター研究会2011
  • Place of Presentation: 千葉
  • Year and Date: 2011-03-07
• [Presentation] Diagnostic soft interface from DNA brush (2011)
  • Author(s): Mizuo Maeda
  • Organizer: International Symposium on Engineering Neo-Biomimetics II
  • Place of Presentation: Ibaraki, Japan
  • Year and Date: 2011-02-26
• [Presentation] Ion-, chemical-, and gene-sensing DNA-modified Au nanoparticles (2011)
  • Author(s): Mizuo Maeda
  • Organizer: 2011 IEEE International Conference on Nano/Micro Engineered and Molecular Systems
  • Place of Presentation: Taiwan
  • Year and Date: 2011-02-23
• [Presentation] Single-molecule imaging of DNA duplex immobilized on surfaces with scanning tunneling microscope (2010)
  • Author(s): T.Ohshiro, M.Maeda
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2010-12-18
• [Presentation] Structural characterization of PNIPAAm-DNA conjugate by small-angle X-ray scattering (2010)
  • Author(s): W.Y.Ooi, M.Fujita, K.Sudesh, M.Maeda
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2010-12-18
• [Presentation] DNA-functionalized thermoresponsive amphiphilic copolymers : Preparation and self-assembly (2010)
  • Author(s): P.Pan, M.Fujita, M.Maeda
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2010-12-18
• [Presentation] Structural study on noncrosslinking aggregation of gold nanoparticles with DNA (2010)
  • Author(s): M.Fujita, M.Maeda
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2010-12-16
• [Presentation] Structural study on noncrosslinking aggregation of gold nanoparticles with DNA (2010)
  • Author(s): M. Fujita
  • Organizer: Pacifichem2010
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-06
• [Presentation] ソフトインターフェースの特異な物性と新機能 (2010)
  • Author(s): 前田瑞夫
  • Organizer: 第10回東北大学多元物質科学研究所研究発表会
  • Place of Presentation: 仙台市(招待講演)
  • Year and Date: 2010-12-01
• [Presentation] Unique colloidal behavior of DNA-carrying nanoparticles (2010)
  • Author(s): Mizuo Maeda
  • Organizer: International Conference on Nanoscopic Colloid and Surface
  • Place of Presentation: Chiba, Japan
  • Year and Date: 2010-09-22
• [Presentation] Unique colloidal behavior of DNA-carrying nanoparticles (2010)
  • Author(s): M.Maeda
  • Organizer: International Conference on Nanoscopic Colloid and Surface
  • Place of Presentation: 千葉市(招待講演)
  • Year and Date: 2010-09-22
• [Presentation] DNA-co-poly(N-isopropylacryl amide) ther moresponsive bioconjugates : Preparation and self-assembly (2010)
  • Author(s): 潘鵬挙, 藤田雅弘, 後藤淳, 宝田徹, 前田瑞夫
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-16
• [Presentation] DNA-co-poly (N-isopropylacrylamide) thermoresponsive bioconjugates : Preparation and self-assembly (2010)
  • Author(s): 潘鵬挙, 藤田雅弘, 後藤淳, 宝田徹, 前田瑞夫
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-16
• [Presentation] ポリ(N-イソプロピルアクリルアミド)-DNAブロック共重合体の自己組織化 (2010)
  • Author(s): 磯田恭佑, 金山直樹, 宝田徹, 前田瑞夫
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-16
• [Presentation] DNA担持金ナノ粒子の非架橋型凝集の特性解析 (2010)
  • Author(s): 小林奈央, 佐藤香枝, 伊藤和輝, 宝田徹, 藤田雅弘, 前田瑞夫
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-15
• [Presentation] Structural characterization of temperature-induced PNIPAAm-DNA conjugate colloidal particle (2010)
  • Author(s): W.Y.Ooi, M.Fujita, K.Ito, P.Pan, K.Sudesh, M.Maeda
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-15
• [Presentation] 遺伝子診断実用化の鍵を握るDNAソフト界面技術の開発 (2010)
  • Author(s): 前田瑞夫
  • Organizer: 第55回高分子夏季大学
  • Place of Presentation: 仙台市(招待講演)
  • Year and Date: 2010-07-16
• [Presentation] DNA担持ナノ粒子の特異な挙動とその応用 (2010)
  • Author(s): 前田瑞夫
  • Organizer: ナノオプティクス研究グループ第19回研究討論会
  • Place of Presentation: 東京都新宿区(招待講演)
  • Year and Date: 2010-07-15
• [Presentation] DNAと合成高分子の複合体を用いる遺伝子診断材料 (2010)
  • Author(s): 前田瑞夫
  • Organizer: 平成22年度繊維学会年次大会
  • Place of Presentation: 東京都江戸川区(招待講演)
  • Year and Date: 2010-06-16
• [Presentation] DNA担持金ナノ粒子の非架橋凝集 (2010)
  • Author(s): 小林奈央, 片渕佳純, 佐藤香枝, 伊藤和輝, 宝田徹, 藤田雅弘, 前田瑞夫
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-05-28
• [Presentation] Structural characterization of PNIPAAm-DNA conjugate by small-angle X-ray scattering (2010)
  • Author(s): W.Y.Ooi, M.Fujita, K.Ito P.Pan, K.Sudesh, M.Maeda
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-05-28
• [Presentation] DNA/Poly (N-isopropylacrylamide) thennoresponsive bioconjugates prepared via ATRP and click chemistry (2010)
  • Author(s): 潘鵬挙, 藤田雅弘, 後藤淳, 前田瑞夫
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-05-28
• [Presentation] ポリ(N-イソプロピルアクリルアミド)-DNAブロック共重合体の合成と物性評価 (2010)
  • Author(s): 磯田恭佑, 金山直樹, 宝田徹, 前田瑞夫
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-05-28
• [Presentation] 走査型トンネル顕微鏡による基板上に固定したDNA二重鎖の単分子観察 (2010)
  • Author(s): 大城敬人, 前田瑞夫
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-05-26
• [Presentation] DNA 担持高分子ミセルの特異なコロイド安定性 (2010)
  • Author(s): 宝田徹
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-26
• [Presentation] DNA-Functionalized Thermoresponsive Polymer Micelles : Self-Assembly and Application to DNA Detection (2010)
  • Author(s): Pengju Pan
  • Organizer: Advanced Polymeric Materials and Technology Symposium 2010
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2010-01-26
• [Presentation] ソフド界面が拓く新機能材料 (2010)
  • Author(s): 前田瑞夫
  • Organizer: 東海シンポジウム-高分子が時代を変える-
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-01-15
• [Presentation] ソフトなバイオ界面の特異な性質と新機能創成 (2009)
  • Author(s): 前田瑞夫
  • Organizer: 第26回プラズマ・核融合学会年会
  • Place of Presentation: 京都市
  • Year and Date: 2009-12-01
• [Presentation] 生体高分子鎖集合体としてのDNAソフト界面 (2009)
  • Author(s): 前田瑞夫
  • Organizer: 第60回医用高分子研究会
  • Place of Presentation: 東京本郷
  • Year and Date: 2009-11-10
• [Presentation] DNAブラシがつくるソフト界面の特異な性質 (2009)
  • Author(s): 前田瑞夫
  • Organizer: ゲルワークショップイン熊本
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2009-09-19
• [Presentation] ソフト界面の特異な性質と機能 (2009)
  • Author(s): 前田瑞夫
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2009-09-18
• [Presentation] DNA修飾した金ナノ粒子の非架橋型凝集の構造解析 (2009)
  • Author(s): 片渕佳純
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2009-09-17
• [Presentation] PNIPAAm-DNA共重合体のナノ粒子形成と凝集の構造解析 (2009)
  • Author(s): 藤田雅弘
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2009-09-17
• [Presentation] DNAが形成するソフトな界面の特異物性と機能 (2009)
  • Author(s): 前田瑞夫
  • Organizer: 遺伝子・デリバリー研究会 第9回シンポジウム
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2009-07-11
• [Presentation] DNA conjugate polymers and nanoparticles for rapid and reliable gene sensing (2009)
  • Author(s): Mizuo Maeda
  • Organizer: APBP 2009
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2009-05-22
• [Presentation] Structural Analysis of Non-crosslinking Aggregation of Au Nanoparticles with DNA (2009)
  • Author(s): M. Fujita
  • Organizer: 3rd International Symposium on Interdisciplinary Materials Science for Biomaterials Science
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2009-04-24
• [Presentation] Structural Analysis of Non-crosslinking Aggregation of Au Nanoparticles with DNA (2009)
  • Author(s): 藤田雅弘
  • Organizer: 3^<rd> International Symposium on Interdisciplinary Materials Science for Biomaterials Science
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2009-04-24
• [Presentation] DNA conjugate polymers and nanoparticles (2009)
  • Author(s): Mizuo Maeda
  • Organizer: 14th International Symposium on Recent Advances in Drug Delivery Systems
  • Place of Presentation: Utah, USA
  • Year and Date: 2009-02-16
• [Presentation] DNA Conjugate Polymers and Nanoparticlels (2009)
  • Author(s): M. Maeda
  • Organizer: 14th International Symposium on Recent Advances in Drug Delivery Systems
  • Place of Presentation: Utah, USA
  • Year and Date: 2009-02-16
• [Presentation] DNA Conjugete Polymers and Nanoparticlels (2009)
  • Author(s): Mizuo MAEDA
  • Organizer: 14^<th> International Symposium on Recent Advances in Drug Delivery Systems
  • Place of Presentation: The University of Utha USA
  • Year and Date: 2009-02-16
• [Presentation] DNA nanomaterials for biosensing (2008)
  • Author(s): Mizuo Maeda
  • Organizer: CNBI International Symposium on Nanobiomaterials
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-11-18
• [Presentation] DNA Nanomaterials for Biosensing (2008)
  • Author(s): Mizuo MAEDA
  • Organizer: CNBIナノバイオマテリアル国際シンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2008-11-18
• [Presentation] DNA二重鎖を嘆じした金ナノ粒子を用いる水銀(II)イオンの簡便・迅速検出 (2008)
  • Author(s): 金山 直樹
  • Organizer: 日本バイオマテリアル学会シンポジウム2008
  • Place of Presentation: 東京大学
  • Year and Date: 2008-11-18
• [Presentation] DNA担持金ナノ粒子の凝集メカニズムの解析 (2008)
  • Author(s): 藤田 雅弘
  • Organizer: 日本バイオマテリアル学会シンポジウム2008
  • Place of Presentation: 東京大学
  • Year and Date: 2008-11-18
• [Book] Nanopores for Bioanalytical Applications (2013)
  • Author(s): M. Takemasa
  • Publisher: RSC Publishing
• [Book] 先端バイオマテリアルハンドブック (2012)
  • Author(s): 金山直樹,前田瑞夫
  • Publisher: エヌ・ティー・エス
• [Book] CSJ カレントレビュ-09 金属および半導体ナノ粒子の科学 (2012)
  • Author(s): 金山直樹,前田瑞夫
  • Publisher: 化学同人
• [Book] 先端バイオマテリアルハンドブック (2012)
  • Author(s): 金山直樹
  • Publisher: 株式会社エヌ・ティー・エス
• [Book] 金属および半導体ナノ粒子の科学―新しいナノ材料の機能性と応用展開― (2012)
  • Author(s): 金山直樹
  • Publisher: 化学同人
• [Book] 化学フロンティア(20)新しい地平をひらく分析手法の最前線 (2009)
  • Author(s): 宝田徹,前田瑞夫
  • Publisher: 化学同人
• [Book] ソフトマター ～分子設計・キャラクタリゼーションから機能性材料まで～ (2009)
  • Author(s): 前田瑞夫
  • Publisher: 丸善
• [Book] 化学フロンティア(20)新しい地平をひらく分析手法の最前線 (2009)
  • Author(s): 宝田徹、前田瑞夫
  • Publisher: 化学同人
• [Book] ソフトマター～分子設計・キャラクタリゼーションから機能性材料まで～(高原淳・栗原和枝・前田瑞夫編) (2009)
  • Author(s): 前田瑞夫
  • Publisher: 丸善株式会社
• [Remarks]
  • URL: http://www.riken.jp/lab-www/bioengineering/
• [Remarks] 読売新聞(夕刊・科学欄・最前線)、"ナノ世界で『金』を活用/空気の浄化、遺伝子診断も/DNA 同士の結合、色の変化でわかる"、2012年11月22日.
• [Remarks] 新学術領域研究「ソフトインターフェースの分子科学」ホームページ
  • URL: http://www.riken.jp/soft-kaimen/
• [Remarks] （独）理化学研究所・前田バイオ工学研究室ホームページ
  • URL: http://www.riken.jp/lab-www/bioengineering/index.htm
• [Remarks] ○研究内容又は成果に関するwebページURL
• [Remarks] 新学術領域研究「ソフトインターフェースの分子科学」
  • URL: http://www.riken.jp/soft-kaimen/
• [Remarks] (独)理化学研究所・前田バイオ工学研究室
  • URL: http://www.riken.jp/lab-www/bioengineering/index.htm
• [Remarks] ○研究室公開など一般向け成果公開活動
• [Remarks] (独)理化学研究所・和光研究所一般公開(対象:全員)(2010.4.23)
• [Remarks] 研究内容又は成果に関するwebページURL 新学術領域研究「ソフトインターフェースの分子科学」
  • URL: http://www.riken.jp/soft-kaimen/
• [Remarks] 理化学研究所・前田バイオ工学研究室
  • URL: http://www.riken.jp/lab-www/bioengineering/index.htm
• [Remarks] 研究室公開など一般向け成果公開活動 高分子同友会(対象:企業技術者など)(2010.11.4)、福井県立高志高校理数科(対象:高校生)(2010.10.22) 理化学研究所和光研究所一般公開(対象:全員) (2010.4.17)
• [Remarks] 図書編集「バイオマテリアルの基礎」(日本医学館)(編集者:石原一彦、塙隆夫、前田瑞夫)2010.11.25(総ページ数:259)
• [Remarks] ・新学術領域「ソフトインターフェースの分子科学」に関するwebページURL
  • URL: http://www.riken.jp/soft-kaimen/
• [Remarks] ・新学術領域「ソフトインターフェースの分子科学」に関するwebページURL
  • URL: http://www.riken.jp/soft-kaimen/
• [Remarks] ・本研究領域における活動
• [Remarks] 第1回領域会議(東京) : 2008年12月20日「DNA密生相が示す特異な界面現象の解明と応用」に関する計画研究の説明
• [Remarks] 第1回公開シンポジウム(東京) : 2009年1月27日領域の紹介と本計画研究にかんする趣旨や意義の紹介
• [Patent(Industrial Property Rights)] 重金属イオンの検出法およびそのための試薬 (2008)
  • Inventor(s): 金山直樹、宝田徹、前田瑞夫
  • Industrial Property Rights Holder: 金山直樹、宝田徹、前田瑞夫
  • Industrial Property Number: 2008-257206
  • Filing Date: 2008-10-02