π space control of firefly luciferase

• Project Area: Emergence of highly elaborated pai-space and its function
• Project/Area Number: 20108016
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kyoto University
• Principal Investigator: NAKATSU Toru (NAKATSU TORU) 京都大学, 大学院・薬学研究科, 准教授 (50293949)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥31,460,000 (Direct Cost: ¥24,200,000、Indirect Cost: ¥7,260,000); Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 生物発光 / ルシフェラーゼ / π空間 / オキシルシフェリン / 立体構造 / 発光反応 / X線結晶構造解析 / ルシフェリン / 励起状態 / 量子収率

Research Abstract

Firefly luciferase catalyzes the emission of yellow-green light and the emission color changes to red by the substitution of an amino acid. We determine the Tyr257, Asn231 and Arg220 mutants. Tyr257 mutants decreased the hydrophobic interaction in the active site. Asn231 and Arg220 mutants broak the hydrogen-bonding network of oxyluciferin binding region. In the result, it is supposed that the change of the emission color is caused by the energy loss of the excited oxyluciferin.

Research Products

• [Journal Article] rystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases (2013)
  • Author(s): Ichiyanagi A, Hirokawa K, Gomi K, Nakatsu T, Kato H, Kajiyama N
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 69(Pt 2) Issue: 2 Pages: 130-3
  • DOI: 10.1107/s1744309112051445
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovorans MO-1 (2012)
  • Author(s): Nakano H, Hosokawa A, Tagawa R, Inaka K, Ohta K, Nakatsu T, Kato H, Watanabe K
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 68(Pt 7) Issue: 7 Pages: 757-9
  • DOI: 10.1107/s1744309112018969
  • Peer Reviewed
• [Journal Article] Structural basis for color modulation mechanism of firefly luciferase bioluminescence (2012)
  • Author(s): Kanako Terakado
  • Journal Title: Luminescence Volume: 27 Pages: 164-164
• [Journal Article] Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p (2010)
  • Author(s): Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H
  • Journal Title: EMBO J Volume: 29 Issue: 24 Pages: 4083-4093
  • DOI: 10.1038/emboj.2010.293
  • Peer Reviewed
• [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP (2010)
  • Author(s): Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H
  • Journal Title: Acta Crystallogr D Volume: 66 Issue: 13 Pages: 319-323
  • DOI: 10.1111/j.1742-4658.2009.07072.x
  • Peer Reviewed
• [Journal Article] Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p. (2010)
  • Author(s): Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H.
  • Journal Title: EMBO J. 29 Pages: 4083-4093
  • Peer Reviewed
• [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP. (2010)
  • Author(s): Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H.
  • Journal Title: Acta Crystallogr D 66 Pages: 319-323
  • Peer Reviewed
• [Journal Article] Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p. (2010)
  • Author(s): Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H.
  • Journal Title: EMBO J. Volume: 29 Pages: 4083-4093
  • Peer Reviewed
• [Journal Article] The exquisite structure and reaction mechanism of bacterial Pz-peptidase A toward collagenous peptides : X-ray crystallographic structure analysis of PZ-peptidase a reveals differences from mammalian thimet oligopeptidase. (2010)
  • Author(s): Kawasaki A, Nakano H, Hosokawa A, Nakatsu T, Kato H, Watanabe K.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 34972-34980
  • Peer Reviewed
• [Journal Article] Functional role of the linker region in purified human P-glycoprotein (2009)
  • Author(s): Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H
  • Journal Title: FEBS Journal Volume: 276 Pages: 3504-3516
  • Peer Reviewed
• [Journal Article] Functional role of the linker region in purified human P-glycoprotein. (2009)
  • Author(s): Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H.
  • Journal Title: FEBS Journal. 276 Pages: 3504-3516
  • Peer Reviewed
• [Journal Article] X-ray crystallographic study of an HIV-1 fusion inhibitor with the gp41 S138A substitutio (2009)
  • Author(s): Watabe T, Terakawa Y, Watanabe K, Ohno H, Nakano H, Nakatsu T, Kato H, Izumi K, Kodama E, Matsuoka M, Kitaura K, Oishi S, Fujii N.
  • Journal Title: J Mol Biol. 392 Pages: 657-665
  • Peer Reviewed
• [Journal Article] Structural basis for gibberellin recognition by its receptor GID1 (2008)
  • Author(s): Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M
  • Journal Title: Nature Volume: 456 Issue: 7221 Pages: 520-523
  • DOI: 10.1038/nature07546
  • NAID: 130006990929
  • Peer Reviewed
• [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection (2008)
  • Author(s): Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K
  • Journal Title: J Am Chem Soc Volume: 130 Issue: 39 Pages: 12880-12881
  • DOI: 10.1021/ja8052464
  • Peer Reviewed
• [Journal Article] Structural basis for gibberellin recognition by its receptor (2008)
  • Author(s): Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M.
  • Journal Title: GID1.Nature 456 Pages: 520-523
  • Peer Reviewed
• [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection. (2008)
  • Author(s): Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K.
  • Journal Title: J Am Chem Soc. 130 Pages: 12880-12881
  • Peer Reviewed
• [Journal Article] Structural basis for gibberellin recognition by its receptor GTD1 (2008)
  • Author(s): Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M.
  • Journal Title: Nature 456 Pages: 520-523
  • Peer Reviewed
• [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection (2008)
  • Author(s): Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K.
  • Journal Title: J Am Chem Soc 130 Pages: 12880-12881
  • Peer Reviewed
• [Presentation] The role of hydrogen bonding network around oxyluciferin binding region in firefly luciferase bioluminescence (2013)
  • Author(s): Toru Nakatsu
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: 名古屋大学、（愛知県）
  • Year and Date: 2013-01-22
• [Presentation] ホタル由来ルシフェラーゼのオキシルシフェリンアナログ複合体の高分解能X線結晶構造解析 (2012)
  • Author(s): 中津亨
  • Organizer: 日本生化学会
  • Place of Presentation: マリンメッセ福岡、（福岡県）
  • Year and Date: 2012-12-14
• [Presentation] Structural basis for color modulation mechanism of firefly luciferase bioluminescence (2012)
  • Author(s): Toru Nakatsu
  • Organizer: 17th International Symposium on Bioluminescence and Chemiluminescence
  • Place of Presentation: Guelph, Ontario, Canada
  • Year and Date: 2012-05-28
• [Presentation] 結晶状単層カーボンナノチューブ集合体の作製とその物性 (2012)
  • Author(s): 工藤光、柳和宏、小林春花、末永和知、蓬田陽平、竹延大志、吉宗良祐、中津亨
  • Organizer: 日本物理学会
  • Place of Presentation: 関西学院大学(兵庫県)
  • Year and Date: 2012-03-25
• [Presentation] Production of Crystalline SWCNT aggregates (2012)
  • Author(s): Hikaru Kudo, Kazuhiro Yanagi, Kazutomo Suenaga, Haruka Kobayashi, Taishi Takenobu, Yohei Yomogida、Kunio Hirata, Ryosuke Yoshimune, Toru Nakatsu
  • Organizer: フラーレンナノチューブグラフェン総合シンポジウム
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2012-03-08
• [Presentation] ホタル由来ルシフェラーゼのオキシルシフェリンアナログ複合体の高分解能X線結晶構造解析 (2012)
  • Author(s): 寺角 香菜子, Naumov Panche, 五味 恵子, 梶山 直樹, 加藤 博章, 中津 亨
  • Organizer: 日本生化学会 2012年度年会
  • Place of Presentation: マリンメッセ福岡(福岡県)
• [Presentation] Structural basis for color modulation mechanism of firefly luciferase bioluminescence (2012)
  • Author(s): Kanako Terakado, Ryosuke Yoshimune, Keiko Gomi, Naoki Kajiyama, Hideyuki Ikeuchi, Jun Hiratake, Hiroaki Kato and Toru Nakatsu
  • Organizer: 17th International Symposium on Bioluminescence and Chemiluminescence
  • Place of Presentation: Guelph Ontario, Canada
• [Presentation] ゲンジボタルルシフェラーゼの発光色制御メカニズムの解明 (2011)
  • Author(s): 寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
  • Organizer: 日本結晶学会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-11-25
• [Presentation] Crystal structure of Pex3p-Pex19p complex from methylotrophic yeast Pichia pastoris (2011)
  • Author(s): Hirai Hidenori, Kyoko Fukushima-Egawa, Hiroyuki Shibata, Toru Nakatsu, Hiroaki Kato
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク(大阪府)
  • Year and Date: 2011-06-08
• [Presentation] ゲンジボタルルシファラーゼの蛍光色制御メカニズムの解明 (2011)
  • Author(s): 寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
  • Organizer: 日本結晶学会 2011年度年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] ゲンジボタルルシフェラーゼの発光色決定メカニズムの解明 (2011)
  • Author(s): 寺角 香菜子、吉宗 良祐、五味 恵子、梶山 直樹、池内 秀幸、平竹 潤、加藤 博章、中津 亨
  • Organizer: 日本農芸化学会2011年度年会
  • Place of Presentation: 京都女子大学(京都市)
• [Presentation] (2011)
  • Author(s): 寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
  • Organizer: ゲンジボタルルシフェラーゼの発光色決定メカニズムの解明日本農芸化学会2011年度年会
  • Place of Presentation: 京都女子大学(京都市)
• [Presentation] ホタルルシフェラーゼの発光色決定メカニズムの解明 (2010)
  • Author(s): 吉宗良祐、寺角香菜子、加藤博章、中津亨
  • Organizer: 日本結晶学会平成22年度年会
  • Place of Presentation: 大阪大学コンベンションセンター(大阪府)
• [Presentation] (2010)
  • Author(s): 寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] (2010)
  • Author(s): 吉宗良祐、寺角香菜子、加藤博章、中津亨
  • Organizer: ホタルルシフェラーゼの発光色決定メカニズムの解明日本結晶学会平成22年度年会
  • Place of Presentation: 大阪大学コンベンションセンター(大阪府)
• [Presentation] (2009)
  • Author(s): 寺角香菜子、中津亨
  • Organizer: ホタル・ルシフェラーセによるπ空間制御機構日本生物物理学会
  • Place of Presentation: アスティ徳島(徳島)
  • Year and Date: 2009-10-30
• [Presentation] ホタル・ルシフェラ-ゼによるπ空間制御機構 (2009)
  • Author(s): 寺角香菜子、中津亨
  • Organizer: 日本生物物理学会
  • Place of Presentation: アスティ徳島
  • Year and Date: 2009-10-30
• [Presentation] ヒト由来ペルオキシソーム膜タンパク質輸送因子Pex3pとPex19pの構造基盤 (2009)
  • Author(s): 佐藤康彦, 柴田洋之, 中津亨, 中野博明, 柏山恭範, 今中常雄, 加藤博章
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] メタノール資化性酵母Pichia pastoris由来Pex3-Pex19p相互作用の解析 (2009)
  • Author(s): 平井秀憲, 江川響子, 柴田洋之, 中津亨, 加藤博章
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] ホタルルシフェラーゼは基質の不斉をどのように見分けているのか? (2009)
  • Author(s): 加藤太一郎, 宮永佳奈, 番匠亜沙美, 中津亨, 武尾正弘, 根来誠司
  • Organizer: 日本生物工学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-24
• [Presentation] ジベレリン受容体の立体構造に基づく分子進化と基質認識機構の解明 (2009)
  • Author(s): 島田麻子、上口(田中)美弥子、中津亨、中嶋正敏、直江洋一、大宮博子、加藤博章、松岡信
  • Organizer: 蛋白質科学会
  • Place of Presentation: 熊本全日空ホテルニュースカイ
  • Year and Date: 2009-05-20
• [Presentation] ホタルにおける発光制御メカニズム (2009)
  • Author(s): 中津亨
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 福岡国際会議場(福岡市)
• [Presentation] (2009)
  • Author(s): 中津亨
  • Organizer: ホタルにおける発光制御メカニズム日本農芸化学会2008年度大会
  • Place of Presentation: 福岡国際会議場(福岡市)
• [Book] 生体π空間の制御機構の解明と新機能開発ーホタルルシフェラーゼによるπ空間制御機構ー (2013)
  • Author(s): 寺角 香菜子、中津 亨
  • Publisher: シーエムシー出版
• [Book] 高次π空間の創発と機能開発 (2013)
  • Author(s): 寺角香菜子
  • Total Pages: 245
  • Publisher: （株）シーエムシー出版
• [Book] 高機能化ホタル・ルシフェラーゼ創製に向けて (2010)
  • Author(s): 中津亨
  • Publisher: 日本結晶学会