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π space control of firefly luciferase

Planned Research

Project AreaEmergence of highly elaborated pai-space and its function
Project/Area Number 20108016
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKyoto University

Principal Investigator

NAKATSU Toru (NAKATSU TORU)  京都大学, 大学院・薬学研究科, 准教授 (50293949)

Project Period (FY) 2008 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥31,460,000 (Direct Cost: ¥24,200,000、Indirect Cost: ¥7,260,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords生物発光 / ルシフェラーゼ / π空間 / オキシルシフェリン / 立体構造 / 発光反応 / X線結晶構造解析 / ルシフェリン / 励起状態 / 量子収率
Research Abstract

Firefly luciferase catalyzes the emission of yellow-green light and the emission color changes to red by the substitution of an amino acid. We determine the Tyr257, Asn231 and Arg220 mutants. Tyr257 mutants decreased the hydrophobic interaction in the active site. Asn231 and Arg220 mutants broak the hydrogen-bonding network of oxyluciferin binding region. In the result, it is supposed that the change of the emission color is caused by the energy loss of the excited oxyluciferin.

Report

(7 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (44 results)

All 2013 2012 2011 2010 2009 2008

All Journal Article (18 results) (of which Peer Reviewed: 17 results) Presentation (23 results) Book (3 results)

  • [Journal Article] rystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases2013

    • Author(s)
      Ichiyanagi A, Hirokawa K, Gomi K, Nakatsu T, Kato H, Kajiyama N
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun

      Volume: 69(Pt 2) Issue: 2 Pages: 130-3

    • DOI

      10.1107/s1744309112051445

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovorans MO-12012

    • Author(s)
      Nakano H, Hosokawa A, Tagawa R, Inaka K, Ohta K, Nakatsu T, Kato H, Watanabe K
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun

      Volume: 68(Pt 7) Issue: 7 Pages: 757-9

    • DOI

      10.1107/s1744309112018969

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for color modulation mechanism of firefly luciferase bioluminescence2012

    • Author(s)
      Kanako Terakado
    • Journal Title

      Luminescence

      Volume: 27 Pages: 164-164

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p2010

    • Author(s)
      Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H
    • Journal Title

      EMBO J

      Volume: 29 Issue: 24 Pages: 4083-4093

    • DOI

      10.1038/emboj.2010.293

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP2010

    • Author(s)
      Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H
    • Journal Title

      Acta Crystallogr D

      Volume: 66 Issue: 13 Pages: 319-323

    • DOI

      10.1111/j.1742-4658.2009.07072.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p.2010

    • Author(s)
      Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H.
    • Journal Title

      EMBO J. 29

      Pages: 4083-4093

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP.2010

    • Author(s)
      Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H.
    • Journal Title

      Acta Crystallogr D 66

      Pages: 319-323

    • Related Report
      2010 Self-evaluation Report 2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p.2010

    • Author(s)
      Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H.
    • Journal Title

      EMBO J.

      Volume: 29 Pages: 4083-4093

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The exquisite structure and reaction mechanism of bacterial Pz-peptidase A toward collagenous peptides : X-ray crystallographic structure analysis of PZ-peptidase a reveals differences from mammalian thimet oligopeptidase.2010

    • Author(s)
      Kawasaki A, Nakano H, Hosokawa A, Nakatsu T, Kato H, Watanabe K.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 34972-34980

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of the linker region in purified human P-glycoprotein2009

    • Author(s)
      Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H
    • Journal Title

      FEBS Journal

      Volume: 276 Pages: 3504-3516

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of the linker region in purified human P-glycoprotein.2009

    • Author(s)
      Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H.
    • Journal Title

      FEBS Journal. 276

      Pages: 3504-3516

    • Related Report
      2010 Self-evaluation Report 2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X-ray crystallographic study of an HIV-1 fusion inhibitor with the gp41 S138A substitutio2009

    • Author(s)
      Watabe T, Terakawa Y, Watanabe K, Ohno H, Nakano H, Nakatsu T, Kato H, Izumi K, Kodama E, Matsuoka M, Kitaura K, Oishi S, Fujii N.
    • Journal Title

      J Mol Biol. 392

      Pages: 657-665

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for gibberellin recognition by its receptor GID12008

    • Author(s)
      Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M
    • Journal Title

      Nature

      Volume: 456 Issue: 7221 Pages: 520-523

    • DOI

      10.1038/nature07546

    • NAID

      130006990929

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection2008

    • Author(s)
      Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K
    • Journal Title

      J Am Chem Soc

      Volume: 130 Issue: 39 Pages: 12880-12881

    • DOI

      10.1021/ja8052464

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for gibberellin recognition by its receptor2008

    • Author(s)
      Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M.
    • Journal Title

      GID1.Nature 456

      Pages: 520-523

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection.2008

    • Author(s)
      Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K.
    • Journal Title

      J Am Chem Soc. 130

      Pages: 12880-12881

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Structural basis for gibberellin recognition by its receptor GTD12008

    • Author(s)
      Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M.
    • Journal Title

      Nature 456

      Pages: 520-523

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection2008

    • Author(s)
      Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K.
    • Journal Title

      J Am Chem Soc 130

      Pages: 12880-12881

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] The role of hydrogen bonding network around oxyluciferin binding region in firefly luciferase bioluminescence2013

    • Author(s)
      Toru Nakatsu
    • Organizer
      Nagoya Symposium Frontiers in Structural Physiology
    • Place of Presentation
      名古屋大学、(愛知県)
    • Year and Date
      2013-01-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] ホタル由来ルシフェラーゼのオキシルシフェリンアナログ複合体の高分解能X線結晶構造解析2012

    • Author(s)
      中津亨
    • Organizer
      日本生化学会
    • Place of Presentation
      マリンメッセ福岡、(福岡県)
    • Year and Date
      2012-12-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structural basis for color modulation mechanism of firefly luciferase bioluminescence2012

    • Author(s)
      Toru Nakatsu
    • Organizer
      17th International Symposium on Bioluminescence and Chemiluminescence
    • Place of Presentation
      Guelph, Ontario, Canada
    • Year and Date
      2012-05-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] 結晶状単層カーボンナノチューブ集合体の作製とその物性2012

    • Author(s)
      工藤光、柳和宏、小林春花、末永和知、蓬田陽平、竹延大志、吉宗良祐、中津亨
    • Organizer
      日本物理学会
    • Place of Presentation
      関西学院大学(兵庫県)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Production of Crystalline SWCNT aggregates2012

    • Author(s)
      Hikaru Kudo, Kazuhiro Yanagi, Kazutomo Suenaga, Haruka Kobayashi, Taishi Takenobu, Yohei Yomogida、Kunio Hirata, Ryosuke Yoshimune, Toru Nakatsu
    • Organizer
      フラーレンナノチューブグラフェン総合シンポジウム
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2012-03-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] ホタル由来ルシフェラーゼのオキシルシフェリンアナログ複合体の高分解能X線結晶構造解析2012

    • Author(s)
      寺角 香菜子, Naumov Panche, 五味 恵子, 梶山 直樹, 加藤 博章, 中津 亨
    • Organizer
      日本生化学会 2012年度年会
    • Place of Presentation
      マリンメッセ福岡(福岡県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Structural basis for color modulation mechanism of firefly luciferase bioluminescence2012

    • Author(s)
      Kanako Terakado, Ryosuke Yoshimune, Keiko Gomi, Naoki Kajiyama, Hideyuki Ikeuchi, Jun Hiratake, Hiroaki Kato and Toru Nakatsu
    • Organizer
      17th International Symposium on Bioluminescence and Chemiluminescence
    • Place of Presentation
      Guelph Ontario, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] ゲンジボタルルシフェラーゼの発光色制御メカニズムの解明2011

    • Author(s)
      寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
    • Organizer
      日本結晶学会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-11-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Crystal structure of Pex3p-Pex19p complex from methylotrophic yeast Pichia pastoris2011

    • Author(s)
      Hirai Hidenori, Kyoko Fukushima-Egawa, Hiroyuki Shibata, Toru Nakatsu, Hiroaki Kato
    • Organizer
      日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2011-06-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] ゲンジボタルルシファラーゼの蛍光色制御メカニズムの解明2011

    • Author(s)
      寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
    • Organizer
      日本結晶学会 2011年度年会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2012 Final Research Report
  • [Presentation] ゲンジボタルルシフェラーゼの発光色決定メカニズムの解明2011

    • Author(s)
      寺角 香菜子、吉宗 良祐、五味 恵子、梶山 直樹、池内 秀幸、平竹 潤、加藤 博章、中津 亨
    • Organizer
      日本農芸化学会2011年度年会
    • Place of Presentation
      京都女子大学(京都市)
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation]2011

    • Author(s)
      寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
    • Organizer
      ゲンジボタルルシフェラーゼの発光色決定メカニズムの解明日本農芸化学会2011年度年会
    • Place of Presentation
      京都女子大学(京都市)
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] ホタルルシフェラーゼの発光色決定メカニズムの解明2010

    • Author(s)
      吉宗良祐、寺角香菜子、加藤博章、中津亨
    • Organizer
      日本結晶学会平成22年度年会
    • Place of Presentation
      大阪大学コンベンションセンター(大阪府)
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation]2010

    • Author(s)
      寺角香菜子、吉宗良祐、五味恵子、梶山直樹、池内秀幸、平竹潤、加藤博章、中津亨
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2012 Final Research Report 2010 Annual Research Report 2010 Self-evaluation Report
  • [Presentation]2010

    • Author(s)
      吉宗良祐、寺角香菜子、加藤博章、中津亨
    • Organizer
      ホタルルシフェラーゼの発光色決定メカニズムの解明日本結晶学会平成22年度年会
    • Place of Presentation
      大阪大学コンベンションセンター(大阪府)
    • Related Report
      2010 Self-evaluation Report
  • [Presentation]2009

    • Author(s)
      寺角香菜子、中津亨
    • Organizer
      ホタル・ルシフェラーセによるπ空間制御機構日本生物物理学会
    • Place of Presentation
      アスティ徳島(徳島)
    • Year and Date
      2009-10-30
    • Related Report
      2012 Final Research Report 2010 Self-evaluation Report
  • [Presentation] ホタル・ルシフェラ-ゼによるπ空間制御機構2009

    • Author(s)
      寺角香菜子、中津亨
    • Organizer
      日本生物物理学会
    • Place of Presentation
      アスティ徳島
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト由来ペルオキシソーム膜タンパク質輸送因子Pex3pとPex19pの構造基盤2009

    • Author(s)
      佐藤康彦, 柴田洋之, 中津亨, 中野博明, 柏山恭範, 今中常雄, 加藤博章
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] メタノール資化性酵母Pichia pastoris由来Pex3-Pex19p相互作用の解析2009

    • Author(s)
      平井秀憲, 江川響子, 柴田洋之, 中津亨, 加藤博章
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホタルルシフェラーゼは基質の不斉をどのように見分けているのか?2009

    • Author(s)
      加藤太一郎, 宮永佳奈, 番匠亜沙美, 中津亨, 武尾正弘, 根来誠司
    • Organizer
      日本生物工学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] ジベレリン受容体の立体構造に基づく分子進化と基質認識機構の解明2009

    • Author(s)
      島田麻子、上口(田中)美弥子、中津亨、中嶋正敏、直江洋一、大宮博子、加藤博章、松岡信
    • Organizer
      蛋白質科学会
    • Place of Presentation
      熊本全日空ホテルニュースカイ
    • Year and Date
      2009-05-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホタルにおける発光制御メカニズム2009

    • Author(s)
      中津亨
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2012 Final Research Report
  • [Presentation]2009

    • Author(s)
      中津亨
    • Organizer
      ホタルにおける発光制御メカニズム日本農芸化学会2008年度大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2010 Self-evaluation Report
  • [Book] 生体π空間の制御機構の解明と新機能開発ーホタルルシフェラーゼによるπ空間制御機構ー2013

    • Author(s)
      寺角 香菜子、中津 亨
    • Publisher
      シーエムシー出版
    • Related Report
      2012 Final Research Report
  • [Book] 高次π空間の創発と機能開発2013

    • Author(s)
      寺角香菜子
    • Total Pages
      245
    • Publisher
      (株)シーエムシー出版
    • Related Report
      2012 Annual Research Report
  • [Book] 高機能化ホタル・ルシフェラーゼ創製に向けて2010

    • Author(s)
      中津亨
    • Publisher
      日本結晶学会
    • Related Report
      2012 Final Research Report

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Published: 2008-04-01   Modified: 2018-03-28  

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