|Budget Amount *help
¥143,260,000 (Direct Cost: ¥110,200,000、Indirect Cost: ¥33,060,000)
Fiscal Year 2012: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2011: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2010: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2009: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2008: ¥56,030,000 (Direct Cost: ¥43,100,000、Indirect Cost: ¥12,930,000)
Nucleosome is the primary building block for chromatin, which packages huge genomic DNA. Histones are protein components of the nucleosome, and several non-allelic histone variants have been identified in higher eukaryotes. In this study, we determined the crystal structures of human nucleosomes, containing one of each histone H3 variants, H3.1, H3.2, H3.3, H3T, or CENP-A. We also identified novel histone variants, which promote nucleosome assembly. These findings provide important information to understand the chromatin architecture with functional nucleosome dynamics.