Analysis of chromatin structure changes during differentiation

• Project Area: The physicochemical field for genetic activities
• Project/Area Number: 20114007
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: SUEMORI Hirofumi 京都大学, 再生医科学研究所, 准教授 (90261198)
• Co-Investigator(Kenkyū-buntansha): YAMAGATA Kazuo 大阪大学, 微生物病研究所, 准教授 (10361312)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥113,100,000 (Direct Cost: ¥87,000,000、Indirect Cost: ¥26,100,000); Fiscal Year 2012: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2011: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2010: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2009: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000); Fiscal Year 2008: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
• Keywords: 細胞分化 / 多能性幹細胞 / 遺伝子発現 / クロマチン / ヒストン修飾 / ES細胞

Research Abstract

To elucidate molecular mechanisms of gene expression regulation in cell differentiation during early development of mammals, we tried to establish new visualizing system of changes inchromatin structure in combination with directed induction of differentiation from human pluripotent stem cells. We also analyzed early cleavage stage embryo by using live cell imaging system.

Research Products

• [Journal Article] The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells (2013)
  • Author(s): Takase O, Yoshikawa M, Idei M, Hirahashi J, Fujita T, Takato T, Isagawa T, Nagae G, Suemori H, Aburatani H, Hishikawa K
  • Journal Title: PLoS One Volume: 8(2) Issue: 2 Pages: e56399-e56399
  • DOI: 10.1371/journal.pone.0056399
  • Peer Reviewed
• [Journal Article] Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging (2013)
  • Author(s): Shimozawa T, Yamagata K, Kondo T, Hayashi S, Shitamukai A, Konno D, Matsuzaki F, Takayama J, Onami S, Nakayama H, Kosugi Y, Watanabe TM, Fujita K, Mimori-Kiyosue Y
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Pages: 3399-404
  • Peer Reviewed
• [Journal Article] 4D imaging reveals a shift in chromosome segregation dynamics during mouse pre-implantation development. (2013)
  • Author(s): Yamagata K
  • Journal Title: Cell Cycle Volume: 12 Issue: 1 Pages: 157-165
  • DOI: 10.4161/cc.23052
  • Peer Reviewed
• [Journal Article] Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging. (2013)
  • Author(s): Togo Shimozawa et al.
  • Journal Title: Proceeding of the National Academy of Sciences of the United States of America Volume: 110 Issue: 9 Pages: 3399-3404
  • DOI: 10.1073/pnas.1216696110
  • Peer Reviewed
• [Journal Article] Long-term Live-cell Imaging of Mammalian Preimplantation Development and Derivation Process of Pluripotent Stem Cells from the Embryos. (2013)
  • Author(s): Yamagata, K. and Ueda, J.
  • Journal Title: Dev. Growth Differ Volume: 55 Issue: 4 Pages: 378-389
  • DOI: 10.1111/dgd.12048
  • NAID: 40019734964
  • Peer Reviewed
• [Journal Article] Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells (2012)
  • Author(s): Miyazaki T, Futaki S, Suemori H, Taniguchi Y, Yamada M, Kawasaki M, Hayashi M, Kumagai H, Nakatsuji N, Sekiguchi K, Kawase E
  • Journal Title: Nat Commun Volume: 3 Issue: 1 Pages: 1236-1236
  • DOI: 10.1038/ncomms2231
  • NAID: 120006626418
  • Peer Reviewed
• [Journal Article] The SMAD2/3 corepressor SNON maintains pluripotency through selective repression of mesendodermal genes in human ES cells (2012)
  • Author(s): Tsuneyoshi N, Tan EK, Sadasivam A, Poobalan Y, Sumi T, Nakatsuji N, Suemori H, Dunn NR
  • Journal Title: Genes Dev Volume: Nov 15; 26(22) Issue: 22 Pages: 2471-6
  • DOI: 10.1101/gad.201772.112
  • Peer Reviewed
• [Journal Article] Dynamic link between histone H3 acetylation and an increase in the functional characteristics of human ESC/iPSC-derived cardiomyocytes (2012)
  • Author(s): Otsuji TG, Kurose Y, Suemori H, Tada M, Nakatsuji N
  • Journal Title: PLoS One Volume: 7(9) Issue: 9 Pages: e45010-e45010
  • DOI: 10.1371/journal.pone.0045010
  • Peer Reviewed
• [Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage (2012)
  • Author(s): Ryo Kamimura, Takamichi Ishii, Naoya Sasaki, Masatoshi Kajiwara, Takafumi Machimoto, Miwa Saito, Kohno K, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Kentaro Yasuchik, Shinji Uemoto
  • Journal Title: Cell Transplant Volume: 21(11) Issue: 11 Pages: 2351-62
  • DOI: 10.3727/096368912x636957
  • Peer Reviewed
• [Journal Article] Prolonged maturation culture favors a reduction in the tumorigenicity and the dopaminergic function of human ESC-derived neural cells in a primate model of Parkinson's disease (2012)
  • Author(s): Doi D, Morizane A, Kikuchi T, Onoe H, Hayashi T, Kawasaki T, Motono M, Sasai Y, Saiki H, Gomi M, Yoshikawa T, Hayashi H, Shinoyama M, Refaat MM, Suemori H, Miyamoto S, Takahashi J.
  • Journal Title: Stem Cells Volume: 30(5) Issue: 5 Pages: 935-45
  • DOI: 10.1002/stem.1060
  • Peer Reviewed
• [Journal Article] Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent. (2012)
  • Author(s): Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T
  • Journal Title: Plos One Volume: 7 Issue: 10 Pages: 1-9
  • DOI: 10.1371/journal.pone.0047512
  • Peer Reviewed
• [Journal Article] Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling (2011)
  • Author(s): Hayashi-Takanaka Y, Yamagata K, Wakayama T, Stasevich TJ, Kainuma T, Tsurimoto T, Tachibana M, Shinkai Y, Kurumizaka H, Nozaki N, Kimura H
  • Journal Title: Nucleic Acids Res Volume: 39 Pages: 6475-88
  • Peer Reviewed
• [Journal Article] Tissue-specific demethylation in CpG-poor promoters during cellular differentiation (2011)
  • Author(s): Nagae G, Isagawa T, Shiraki N, Fujita T, Yamamoto S, Tsutsumi S, Nonaka A, Yoshiba S, Matsusaka K, Midorikawa Y, Ishikawa S, Soejima H, Fukayama M, Suemori H, Nakatsuji N, Kume S, Aburatani H
  • Journal Title: Hum Mol Genet Volume: 20(14) Pages: 2710-21
  • Peer Reviewed
• [Journal Article] Efficient and Accurate Homologous Recombination in hESCs and hiPSCs Using Helper-dependent Adenoviral Vectors (2011)
  • Author(s): Aizawa E, Hirabayashi Y, Iwanaga Y, Suzuki K, Sakurai K, Shimoji M, Aiba K, Wada T, Tooi N, Kawase E, Suemori H, Nakatsuji N, Mitani K
  • Journal Title: Mol Ther Volume: 6 Issue: 2 Pages: 424-431
  • DOI: 10.1038/mt.2011.266
  • Peer Reviewed
• [Journal Article] Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage (2011)
  • Author(s): Amps, K., et al
  • Journal Title: Nature Biotechnology Volume: 29 Issue: 12 Pages: 1132-1144
  • DOI: 10.1038/nbt.2051
  • Peer Reviewed
• [Journal Article] Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy (2011)
  • Author(s): Yamagata K
  • Journal Title: PLoS One Volume: 7 Issue: 2 Pages: e31638-e31638
  • DOI: 10.1371/journal.pone.0031638
  • Peer Reviewed
• [Journal Article] Simple and highly efficient method for production of endothelial cells from human embryonic stem cells (2011)
  • Author(s): Tatsumi R, Suzuki Y, Sumi T, Sone M, Suemori H, Nakatsuji N
  • Journal Title: Cell Transplant Volume: 20 Issue: 9 Pages: 1423-1430
  • DOI: 10.3727/096368910x547444
  • Peer Reviewed
• [Journal Article] Alpha-fetoprotein-producing pancreatic cancer cells possess cancer stem cell characteristics (2011)
  • Author(s): Sasaki N, Ishii T, Kamimura R, Kajiwara M, Machimoto T, Nakatsuji N, Suemori H, Ikai I, Yasuchika K, Uemoto S
  • Journal Title: Cancer Lett Volume: 308 Issue: 2 Pages: 152-161
  • DOI: 10.1016/j.canlet.2011.04.023
  • NAID: 120003290284
  • Peer Reviewed
• [Journal Article] In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers (2010)
  • Author(s): Ishii T, Yasuchika K, Fukumitsu K, Kawamoto T, Kawamura-Saitoh M, Amagai Y, Ikai I, Uemoto S, Kawase E, Suemori H, Nakatsuji N
  • Journal Title: Cell Tissue Res Volume: 339(3) Issue: 3 Pages: 505-12
  • DOI: 10.1007/s00441-009-0906-7
  • NAID: 120002511339
  • Peer Reviewed
• [Journal Article] Cardiomyocytes develop from anterior primitive streak cells induced by β-catenin activation and the blockage of BMP signaling in hESCs (2010)
  • Author(s): Yamauchi K, Sumi T, Minami I, Otsuji T.G, Kawase E, Nakatsuji N, Suemori H
  • Journal Title: Genes to Cells Volume: 15(12) Issue: 2 Pages: 1216-27
  • DOI: 10.1016/j.cryobiol.2009.10.007
  • Peer Reviewed
• [Journal Article] Role of SOX2 in maintaining pluripotency of human embryonic stem cells. (2010)
  • Author(s): Adachi K, Suemori H, Yasuda SY, Nakatsuji N, Kawase E.
  • Journal Title: Genes Cells 15 Pages: 455-470
  • Peer Reviewed
• [Journal Article] International Stem Cell Initiative Consortium, Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells. (2010)
  • Author(s): Akopian V, Andrews PW, Beil S, Benvenisty N, Brehm J, Christie M, Ford A, Fox V, Gokhale PJ, Healy L, Holm F, Hovatta O, Knowles BB, Ludwig TE, McKay RD, Miyazaki T, Nakatsuji N, Oh SK, Pera MF, Rossant J, Stacey GN, Suemori H.
  • Journal Title: In Vitro Cell Dev Biol Anim. 46 Pages: 247-258
  • Peer Reviewed
• [Journal Article] In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers. (2010)
  • Author(s): Ishii T, Yasuchika K, Fukumitsu K, Kawamoto T, Kawamura-Saitoh M, Amagai Y, Ikai I, Uemoto S, Kawase E, Suemori H, Nakatsuji N.
  • Journal Title: Cell Tissue Res. 339 Pages: 505-512
  • NAID: 120002511339
  • Peer Reviewed
• [Journal Article] Efficient integration of transgenes into a defined locus in human embryonic stem cells (2010)
  • Author(s): Sakurai K, Shimoji M, Tahimic CG, Aiba K, Kawase E, Hasegawa K, Amagai Y, Suemori H, Nakatsuji N
  • Journal Title: Nucleic Acids Research. Volume: 38
  • Peer Reviewed
• [Journal Article] Alpha-fetoprotein producing cells act as cancer progenitor cells in human cholangiocarcinoma (2010)
  • Author(s): Ishii T, Yasuchika K, Suemori H, Nakatsuji N, Ikai I, Uemoto S
  • Journal Title: Cancer Letter Volume: 294 Pages: 25-34
  • Peer Reviewed
• [Journal Article] Role of SOX2 in maintaining pluripotency of human embryonic stem cells (2010)
  • Author(s): Adachi K, Suemori H, Yasuda SY, Nakatsuji N, Kawase E
  • Journal Title: Genes to Cells Volume: 15 Pages: 455-470
  • Peer Reviewed
• [Journal Article] Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells (2010)
  • Author(s): Ohgushi M, Matsumura M, Eiraku M, Murakami K, Aramaki T, Nishiyama A, Muguruma K, Nakano T, Suga H, Ueno M, Ishizaki T, Suemori H, Narumiya S, Niwa H, Sasai Y
  • Journal Title: Cell Stem Cell Volume: 7 Pages: 225-239
  • Peer Reviewed
• [Journal Article] Cardiomyocytes develop from anterior primitive streak cells induced by ss-catenin activation and the blockage of BMP signaling in hESCs (2010)
  • Author(s): Yamauchi K, Sumi T, Minami I, Otsuji G.T, Kawase E, Nakatsuji N, Suemori H
  • Journal Title: Genes to Cells Volume: 15 Pages: 1216-1227
  • Peer Reviewed
• [Journal Article] 医療応用に適したES細胞培養システム (2010)
  • Author(s): 高田圭、末盛博文
  • Journal Title: 実験医学 28 Pages: 204-208
• [Journal Article] Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells (2009)
  • Author(s): Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, Ebihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K
  • Journal Title: FEBS Lett Volume: 583(6) Issue: 6 Pages: 1029-33
  • DOI: 10.1016/j.febslet.2009.02.031
  • Peer Reviewed
• [Journal Article] In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells (2009)
  • Author(s): Yamauchi K, Hasegawa K, Chuma S, Nakatsuji N, Suemori H
  • Journal Title: PLoS ONE Volume: 4(4) Issue: 4 Pages: e5338-e5338
  • DOI: 10.1371/journal.pone.0005338
  • Peer Reviewed
• [Journal Article] Gene targeting in human pluripotent stem cells with adeno-associated virus vectors. (2009)
  • Author(s): Mitsui K, Suzuki K, Aizawa E, Kawase E, Suemori H, Nakatsuji N, Mitani K.
  • Journal Title: Biochem.Biophys.Res.Commun. 388 Pages: 711-717
  • Peer Reviewed
• [Journal Article] Adipogenic differentiation of human induced pluripotent stem cells : comparison with that of human embryonic stem cells. (2009)
  • Author(s): Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, E bihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K.
  • Journal Title: FEBS Lett. 583 Pages: 1029-1033
  • Peer Reviewed
• [Journal Article] In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells. (2009)
  • Author(s): Yamauchi K, Hasegawa K, Chuma S, Nakatsuji N, Suemori H.
  • Journal Title: PLoS ONE. 4 Pages: 5338-5338
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Fukumitsu K, Ishii T, Yasuchika K, Amagai Y, Saitoh M, Kawamoto T, Kawase E, Suemori H, Nakatsuji N, Ikai I, Uemoto S.
  • Journal Title: Tissue Eng Part A. 15 Pages: 3847-3856
  • Peer Reviewed
• [Journal Article] Gene targeting in human pluripotent stem cells with adeno-associated virus vectors. (2009)
  • Author(s): Mitsui K, Suzuki K, Aizawa E, Kawase E, Suemori H, Nakatsuji N, Mitani K.
  • Journal Title: Biochem Biophys Res Commun. 388 Pages: 711-717
  • Peer Reviewed
• [Presentation] ラミニンE8フラグメントを用いたヒトES/iPS細胞の単一分散培養法 (2013)
  • Author(s): 宮崎隆道
  • Organizer: 第１２回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-21
• [Presentation] Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones. (2013)
  • Author(s): 山縣一夫
  • Organizer: The 10th NIBB-EMBL Symposium 2013, Quantitative Bioimaging
  • Place of Presentation: 岡崎
  • Year and Date: 2013-03-17
• [Presentation] ラミニン E8フラグメントを用いたヒトES/iPS細胞の単一分散培養法宮崎隆道 (2013)
  • Author(s): 二木杉子,末盛博文,谿口征雅,山田雅司,川崎美和,林麻利亜,熊谷英明,中辻憲夫,関口清俊,川瀬栄八郎
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones (2013)
  • Author(s): 山縣一夫
  • Organizer: The 10th NIBB-EMBL Symposium 2013, Quantitative Bioimaging
  • Place of Presentation: 岡崎
• [Presentation] 蛍光プローブ(KP-1)のES/iPS細胞特異的蓄積におけるABCタンパク質の関与 (2012)
  • Author(s): 冨岡麻衣子
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] 哺乳動物初期胚卵割時における染色体分配異常について (2012)
  • Author(s): 山縣一夫
  • Organizer: 遺伝研研究会「染色体ドメインの形成と機能発現機構」
  • Place of Presentation: 三島
  • Year and Date: 2012-10-18
  • Invited
• [Presentation] ＥＳ細胞のできるまで (2012)
  • Author(s): 山縣一夫
  • Organizer: 第105回日本繁殖生物学会大会
  • Place of Presentation: 筑波
  • Year and Date: 2012-09-06
• [Presentation] マウス体細胞クローンの低成功率は、初期卵割時の染色体分配異常に起因する (2012)
  • Author(s): 山縣一夫
  • Organizer: 第30回日本受精着床学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2012-08-30
  • Invited
• [Presentation] Recombinant E8 fragments of human laminin isoforms support the efficient adhesion and expansion of dissociated human pluripotent stem cells under defined and xeno-free condition. (2012)
  • Author(s): Takamichi Miyazaki
  • Organizer: ISSCR2012 (国際幹細胞学会 第10回年次大会)
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-13
• [Presentation] 哺乳動物初期胚の質を定量的に見る（Quantification of embryo quality） (2012)
  • Author(s): 山縣一夫
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-29
  • Invited
• [Presentation] ライブセルイメージングによる初期胚発生核ダイナミクスの可視化（Visualization of nuclear dynamics of preimplantation development by live-cell imaging.） (2012)
  • Author(s): 山縣一夫
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-14
  • Invited
• [Presentation] 哺乳動物初期胚の質を定量化してみる (2012)
  • Author(s): 山縣一夫
  • Organizer: 第4回定量生物学の会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-01-09
• [Presentation] Recombinant E8 fragments of human laminin isoforms support the efficient adhesion and expansion of dissociated human pluripotent stem cells under defined and xeno-free condition (2012)
  • Author(s): Takamichi Miyazaki, Sugiko Futaki, Hirofumi Suemori, Yukimasa Taniguchi, Masashi Yamada, Miwa Kawasaki, Maria Hayashi, Hideaki Kumagai, Norio Nakatsuji, Kiyotoshi Sekiguchi, Eihachiro Kawase
  • Organizer: ISSCR2012 (国際幹細胞学会第10回年次大会)
  • Place of Presentation: 横浜
• [Presentation] 割時の染色体分配異常に起因する (2012)
  • Author(s): 山縣一夫
  • Organizer: 第30回日本受精着床学会総会
  • Place of Presentation: 大阪
• [Presentation] 哺乳動物初期胚の質を定量的に見る(Quantification of embryo quality) (2012)
  • Author(s): 山縣一夫
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
• [Presentation] ライブセルイメージングによる初期胚発生核ダイナミクスの可視化(Visualization of nuclear dynamics of preimplantationdevelopment by live-cell imaging.) (2012)
  • Author(s): 山縣一夫
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] ヒトES細胞からdefinitive endodermへの高率な誘導方法の構築 (2011)
  • Author(s): 武内大輝、中辻憲夫、末盛博文
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] ヒトES細胞からの原条形成過程におけるクロマチン修飾因子の発現解析 (2011)
  • Author(s): 末盛博文
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2011-12-07
• [Presentation] Genetic and Epigenetic Landscape of Mouse Preimplantation Development Revealed by Live-cell Imaging (2011)
  • Author(s): 山縣一夫
  • Organizer: 2nd World Congress of Reproductive Biology
  • Place of Presentation: オーストラリア,クイーンズランド州
  • Year and Date: 2011-10-11
• [Presentation] ライブセルイメージングを用いた初期胚のクリティ評価 (2011)
  • Author(s): 山縣一夫
  • Organizer: 勝川 ART研究会
  • Place of Presentation: 春日井
  • Year and Date: 2011-06-22
• [Presentation] Dissection of the early cardiac developmental process from human embryonic stem cells using a model system that recapitulates the embryogenesis (2011)
  • Author(s): Yamauchi K, Nakatsuji N, Suemori H
  • Organizer: International Society for Stem Cell Research 9th Annual Meeting
  • Place of Presentation: Tronto, Canada
  • Year and Date: 2011-06-15
• [Presentation] Cardiomyocytes develop from anterior primitive streak cells induced by β-catenin activation and the blockage of BMP signaling in hESCs (2011)
  • Author(s): Yamauchi K, Sumi T, Minami I, Otsuji G.T, Kawase E, Nakatsuji N, Suemori H
  • Organizer: ISSCR
  • Place of Presentation: トロント、カナダ
  • Year and Date: 2011-06-15
• [Presentation] 単一胚ライブセルイメージングによる初期胚発生核ダイナミクスの可視化 (2011)
  • Author(s): 山縣一夫
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 吹田市
  • Year and Date: 2011-06-08
• [Presentation] Dissection of the early cardiac developmental process from human embryonic stem cells using a model system that recapitulates the embryogenesis (2011)
  • Author(s): Kaori Yamauchi, Norio Nakatsuji and Hirofumi Suemori
  • Organizer: International Society for Stem Cell Research 9th Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] SnoN represses mesendodermal genes in human ES cells (2011)
  • Author(s): Norihiro Tsuneyoshi, Tomoyuki Sumi, Akila Sadasivam, Jennica Tan Ee Kim, Norio Nakatsuji, Hirofumi Suemori
  • Organizer: Norris Ray Dunn Stem Cell Society Singapore (SCSS) Symposium
  • Place of Presentation: Singapore
• [Presentation] ヒト ES細胞から definitive endodermへの高率な誘導方法の構築 (2011)
  • Author(s): 武内大輝、中辻憲夫、末盛博文
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ヒトES細胞から見た多能性幹細胞の特性 (2010)
  • Author(s): 末盛博文
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場(広島)
  • Year and Date: 2010-03-19
• [Presentation] Cardiomyocytes develop from anterior primitive streak cells induced by β-catenin activation and the blockage of BMP signaling in hESCs (2010)
  • Author(s): Kaori Yamauchi, Tomoyuki Sumi, Itsunari Minami, Tomomi G Otsuji, Eihachiro Kawase, Norio Nakatsuji, and Hirofumi Suemori
  • Organizer: 9th ISSCR Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] ヒト ES細胞からの原条形成過程におけるクロマチン修飾因子の発現解析 (2010)
  • Author(s): 末盛博文
  • Organizer: 第33回日本分子生物学会年会ワークショップ
  • Place of Presentation: 神戸
• [Presentation] ヒトES細胞からの原条形成過程におけるクロマチン修飾因子の発現解析 (2010)
  • Author(s): 末盛博文
  • Organizer: 第33回日本分子生物学会年会
• [Presentation] ヒトES細胞から見た多能性幹細胞の特性 (2010)
  • Author(s): 末盛博文
  • Organizer: 第9回日本再生医療学会
• [Presentation] Tissue-specific promoter hypomethylation as a new aspect of tissue-specific differentially methylated regions, T-DMR (2009)
  • Author(s): G Nagae, T Isagawa, N Shiraki, A Kaneda, H Suemori, S Kume, H Aburatan
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Recombinant human laminin isoforms are effective in culture of human embryonic stem cells. (2009)
  • Author(s): T Miyazaki, S Futaki, K Hasegawa, M Kawasaki, N Sanzen, M Hayashi, E Kawase, K Sekiguchi, N Nakatsuji, H Suemori
  • Organizer: International Society for Stem Cell Research, 7th Annual Meeting
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2009-07-10
• [Presentation] Development of a novel xeno-free human embryonic stem cell culture system using small molecules (2009)
  • Author(s): K Hasegawa, JL Teo, H Suemori, N Nakatsuji, MF Pera, M Kahn
  • Organizer: International Society for Stem Cell Research, 7th Annual Meeting
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2009-07-10
• [Presentation] 初めてのヒトES細胞.培養と分化誘導 (2009)
  • Author(s): 末盛博文
  • Organizer: 第8回核ダイナミクス研究会
  • Place of Presentation: 修善寺
  • Year and Date: 2009-06-18
• [Presentation] Recombinant human laminin isoforms are effective in culture of human embryonic stem cells (2009)
  • Author(s): Takamichi Miyazaki, Sugiko Futaki, Kouichi Hasegawa, Miwa Kawasaki, Noriko Sanzen, Maria Hayashi, Eihachiro Kawase, Kiyotoshi Sekiguchi, Norio Nakatsuji, Hirofumi Suemori
  • Organizer: International Society for Stem Cell Research, 7th Annual Meeting
  • Place of Presentation: Barcelona, Spain
• [Presentation] Optimization Of Vector Design For Lentiviral Gene Transfer Into Human Embryonic Stem Cells (2009)
  • Author(s): Emi Aizawa, Kaoru Mitsui, Keiichiro Suzuki, Hirofumi Suemori, Norio Nakatsuji, and Ko Mitani
  • Organizer: American Society of Gene Therapy 12th Annual Meeting
  • Place of Presentation: San Diego USA
• [Presentation] Tissuespe cificpromoter hypomethylation as a newaspect of tissue-specific differentiallymethylated regions, T-DMR. (2009)
  • Author(s): Nagae G, Isagawa T, Shiraki N, Kaneda A, Suemori H, Kume S, Aburatani H.
  • Organizer: 第32回日本分子生物学会年会
• [Presentation] 初めてのヒトES細胞 培養と分化誘導 (2009)
  • Author(s): 末盛博文
  • Organizer: 第8回核ダイナミクス研究会
• [Book] 産科と婦人科 78巻 8号 (2011)
  • Author(s): 糸井史陽、山縣一夫
  • Publisher: (株)診断と治療社
• [Book] 再生医療の最前線 2010「再生医療に適した ES細胞培養システム」 (2010)
  • Author(s): 高田圭、末盛博文
  • Publisher: 実験医学 28
• [Book] 再生医療の最前線2010(再生医療に適したES細胞培養システム実験医学) (2010)
  • Author(s): 高田圭, 末盛博文
• [Remarks] 報道関連,山縣らは、マウス体細胞クローンの低い成功率の原因が、初期胚における卵割時の染色体分配異常に起因することを発見した。この研究成果は、将来高効率なクローン動物作製につながるだけでなく、不妊症解明の基礎的知見として重要であると判断したため、新聞発表を行った(下、新聞記事1)。朝日新聞以外にも、毎日新聞と読売新聞の全国版、神戸新聞、茨城新聞等の地方新聞や各種専門新聞に同様の記事が掲載された。
• [Patent(Industrial Property Rights)] 顕微鏡および光透過ユニット (2011)
  • Inventor(s): 若山照彦、山縣一夫、今井雄一郎、田村恵裕
  • Industrial Property Rights Holder: 独立行政法人理化学研究所、オリンパス株式会社
  • Industrial Property Number: 2011-286996
  • Filing Date: 2011-12-27