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Functional analysis of neuronal circuits that generate locomotion in zebrafish

Planned Research

Project AreaSystems molecular ethology to understand the operating principle of the nervous system
Project/Area Number 20115007
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOkazaki Research Facilities, National Institutes of Natural Sciences

Principal Investigator

HIGASHIJIMA Shinichi (HIGASHIJIMA Shin-Ichi)  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 准教授 (80270479)

Project Period (FY) 2008-11-13 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥84,500,000 (Direct Cost: ¥65,000,000、Indirect Cost: ¥19,500,000)
Fiscal Year 2012: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2011: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2010: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2009: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2008: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Keywordsゼブラフィッシュ / 運動 / 神経回路 / 脊髄 / トランスジェニック
Research Abstract

Neuronal circuits that generate locomotion in mammals are not well understood. In this study, we studied locomotor circuits in larval zebrafish, by using a number of transgenic zebrafish expressing fluorescent protein in a particular class of neurons. We have revealed the followings: (1) a specialized class of commissural inhibitory neurons play a critical role for determining the direction of an escape behavior, (2) multiple types of V0 neurons are produced by two distinct mechanisms; from heterogeneous p0 progenitors and from the same p0 progenitor, but in a time-dependent manner, (3) Chx10-expressing neurons in the hindbrain play an essential role for providing excitation to the spinal locomotor circuits during zebrafish swimming.

Report

(7 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (41 results)

All 2013 2012 2011 2010 2009 2008

All Journal Article (28 results) (of which Peer Reviewed: 28 results) Presentation (13 results)

  • [Journal Article] Hindbrain V2a neurons in the excitation of spinal locomotor circuits during zebrafish swimming2013

    • Author(s)
      Kimura, Y., Satou, C., Fujioka, S., Shoji, W., Umeda, K., Ishizuka, T., Yawo, H., and Higashijima, S.
    • Journal Title

      Current Biology

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgenic tools to characterize neuronal properties of discrete populations of zebrafish neurons2013

    • Author(s)
      Satou, C., Kimura, Y., Hirata, H., Suster, M.L, Kawakami, K., and Higashijima, S.
    • Journal Title

      Development

      Volume: 140 Issue: 18 Pages: 3927-3931

    • DOI

      10.1242/dev.099531

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Origin of excitation underlying locomotion in the spinal circuit of zebrafishOrigin of excitation underlying locomotion in the spinal circuit of zebrafish2012

    • Author(s)
      Eklof-Ljunggren, E., Haupt, S., Ausborn, J., Dehnisch, I., Uhlen, P., Higashijima, S., and El Manira, A.
    • Journal Title

      Proc. Natl. Acad. Sci. (USA)

      Volume: 109 Pages: 5511-5516

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of multiple classes of V0 neurons in zebrafish spinal cord : progenitor heterogeneity and temporal control of neuronal diversity2012

    • Author(s)
      Satou, C., Kimura, Y., and Higashijima, S.
    • Journal Title

      J. Neuroscience

      Volume: 32 Pages: 1771-1783

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of multiple classes of V0 neurons in zebrafish spinal cord : progenitor heterogeneity and temporal control of neuronal diversity2012

    • Author(s)
      Satou, C., Kimura, Y., Higashijima, S.
    • Journal Title

      J.Neuroscience

      Volume: 32 Pages: 1771-1783

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping a sensory-motor network onto a structural and functional ground plan in the hindbrain2011

    • Author(s)
      Koyama, M., Kinkhabwala, A., Satou, C., Higashijima, S., and Fetcho, J.R.
    • Journal Title

      Proc. Natl. Acad. Sci. (USA)

      Volume: 108 Pages: 1170-1175

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A structural and functional ground plan for neurons in the hindbrain of zebrafish2011

    • Author(s)
      Kinkhabwara, A., Riley, M., Koyama, M., Monen, J., Satou, C., Kimura, Y., Higashijima, S., and Fetcho, J.R.
    • Journal Title

      Proc. Natl. Acad. Sci. (USA)

      Volume: 108 Pages: 1164-1169

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mapping a sensory-motor network onto a structural and functional ground plan in the hindbrain2011

    • Author(s)
      Koyama, M., Kinkhabwala, A., Satou, C., Higashijima, S., Fetcho, J.R.
    • Journal Title

      Proc.Natl.Acad.Sci.(USA)

      Volume: 108 Pages: 1170-1175

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A structural and functional ground plan for neurons in the hindbrain of zebrafish2011

    • Author(s)
      Kinkhabwara, A., Riley, M., Koyama, M., Monen, J., Satou, C., Kimura, Y., Higashijima, S., Fetcho, J.R.
    • Journal Title

      Proc.Natl.Acad.Sci.(USA)

      Volume: 108 Pages: 1164-1169

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping a sensory-motor network onto a structural and functional ground plan in the hindbrain.2011

    • Author(s)
      Koyama, M., Kinkhabwala, A., Satou, C., Higashijima, S., Fetcho, J.R.
    • Journal Title

      Proc.Natl.Acad.Sci.(USA) 108

      Pages: 1170-1175

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] A structural and functional ground plan for neurons in the hindbrain of zebrafish.2011

    • Author(s)
      Kinkhabwara, A., Riley, M., Koyama, M., Monen, J., Satou, C., Kimura, Y., Higashijima, S., Fetcho, J.R.
    • Journal Title

      Proc.Natl.Acad.Sci.(USA) 108

      Pages: 1164-1169

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Genetic visualization with an improved GCaMP reveals spatiotemporal activation of the spinal motor neurons in zebrafish2011

    • Author(s)
      Muto, A., Ohkura, M., Kotani, T., Higashijima, S., Nakai, J., Kawakami, K.
    • Journal Title

      Proc.Natl.Acad.Sci.(USA)

      Volume: 108 Pages: 5425-5430

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Compartmentalized Notch signaling sustains epithelialmirror symmetry2011

    • Author(s)
      Wibowo, I., Pinto-Teixeir, F, Satou, C., Higashijima, S., Lopez-Schier, H.
    • Journal Title

      Development

      Volume: 138 Pages: 1143-1152

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The habenula is crucial for experience-dependent modification of fear responses in zebrafish.2010

    • Author(s)
      Agetsuma, M., Aizawa, H., Aoki, T., Nakayama, R., Takahoko, M., Goto, M., Sassa, T., Amo, R., Shiraki, T., Kawakami, K., Hosoya, T., Higashijima, S., Okamoto, H.
    • Journal Title

      Nature Neuroscience 13

      Pages: 1354-1356

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The habenula is crucial for experience-dependent modification of fear responses in zebrafish2010

    • Author(s)
      Agetsuma, M., Aizawa, H., Aoki, T., Nakayama, R., Takahoko, M., Goto, M., Sassa, T., Amo, R., Shiraki, T., Kawakami, K., Hosoya, T., Higashijima, S., Okamoto, H.
    • Journal Title

      Nature Neuroscience

      Volume: 13 Pages: 1354-1356

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of germline stem cells in the ovary of the teleost medaka2010

    • Author(s)
      Nakamura, S., Kobayashi, K., Nishimura, T., Higashijima, S., Tanaka, M.
    • Journal Title

      Science

      Volume: 328 Pages: 1561-1563

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualizing voltage dynamics in zebrafish heart2010

    • Author(s)
      Tsutsui, H., Higashijima, S., Miyawaki, A., Okamura, Y
    • Journal Title

      J.Physiology

      Volume: 588 Pages: 2017-2021

    • NAID

      10030455833

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum2010

    • Author(s)
      Kani, S., Bae, Y-K, Shimizu T, Tanabe, K., Satou, C., Parsons, M., Scott, E., Baier, H., Higashijima, S., Hibi, M.
    • Journal Title

      Developmental Biology

      Volume: 343 Pages: 1-17

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish2010

    • Author(s)
      Wada, H., Ghysen, A., Satou, C., Higashijima, S, Kawakami, K., Hamaguchi, S., Sakaizumi, M.
    • Journal Title

      Developmental Biology

      Volume: 340 Pages: 583-594

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish2009

    • Author(s)
      Satou, C., Kimura, Y., Kohashi, T., Horikawa, K., Takeda, H., Oda, Y., and Higashijima, S.
    • Journal Title

      J. Neuroscience

      Volume: 29 Pages: 6780-6793

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish.2009

    • Author(s)
      Satou, C., Kimura, Y., Kohashi, T., Horikawa, K., Takeda, H., Oda, Y., Higashijima, S.
    • Journal Title

      J.Neuroscience 29

      Pages: 6780-6793

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish2009

    • Author(s)
      Satou, C.
    • Journal Title

      Journal of Neuroscience 29

      Pages: 6780-6793

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] From the Olfactory Bulb to Higher Brain Centers : Genetic Visualization of Secondary Olfactory Pathways in Zebrafish2009

    • Author(s)
      Miyasaka, N.
    • Journal Title

      Joumal of Neuroscience 29

      Pages: 4756-4767

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Illuminating Cell-Cycle Progression in the Developing Zebrafish Embryo2009

    • Author(s)
      Sugiyama, M.
    • Journal Title

      Proc.Natl.Acad.Sci.(USA) 106

      Pages: 20812-20817

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] V2a and V2b neurons are generated by the final divisions of pair-producing progenitors in the zebrafish spinal cord.2008

    • Author(s)
      Kimura, Y., Satou, C., Higashijima, S.
    • Journal Title

      Development 135

      Pages: 3001-3005

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] V2a and V2b neurons are generated by the final divisions of pair- producing progenitors in the zebrafish spinal cord.2008

    • Author(s)
      Kimura, Y.
    • Journal Title

      Development 135

      Pages: 3001-3005

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs.2008

    • Author(s)
      Hossain, Md. I.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 18248-18259

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutation in the abdb gene causes abnormal iron and fatty acid metabolism in developing medaka fish.2008

    • Author(s)
      Miyake, A.
    • Journal Title

      Development, Growth & Differentiation 50

      Pages: 703-716

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish2013

    • Author(s)
      Higashijima, S., Kimura, Y., and Satou, C.
    • Organizer
      第36回日本分子生物学会大会
    • Place of Presentation
      神戸、兵庫県
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish2012

    • Author(s)
      東島、木村、佐藤
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of V2 and V0 neurons in zebrafish spinal cord2012

    • Author(s)
      Higashijima, S.
    • Organizer
      19th biennial meeting of the International Society for Developmental Neuroscience (ISDN-2012)
    • Place of Presentation
      ムンバイ(インド)
    • Year and Date
      2012-01-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish2011

    • Author(s)
      東島、木村、佐藤
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish2011

    • Author(s)
      東島、木村、佐藤
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish2011

    • Author(s)
      Higashijima S, Kimura Y, Satou C
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development and function of V2 neuron in zebrafish spinal cord2010

    • Author(s)
      Higashijima, S.
    • Organizer
      2nd Joint Meeting of the French and Japanese Societies for Developmental Biology
    • Place of Presentation
      パリ(フランス)
    • Year and Date
      2010-05-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Development and function of V2 neuron in zebrafish spinal cord.2nd Joint Meeting of the French and Japanese Societies for Developmental Biology2010

    • Author(s)
      Higashijima, S.
    • Organizer
      2nd Joint Meeting of the French and Japanese Societies for Developmental Biology
    • Place of Presentation
      Institute Pasteur (Paris)
    • Year and Date
      2010-05-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] ゼブラフィッシュの逃避運動に重要な役割を果たす、特殊なクラスの交差型抑制性介在ニューロン2010

    • Author(s)
      東島眞一
    • Organizer
      第87回生理学会大会
    • Place of Presentation
      盛岡市民文化ホール(岩手県盛岡市)
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2009

    • Author(s)
      東島、佐藤、木村
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2009

    • Author(s)
      S Higashijima, Y Kimura, C Satou
    • Organizer
      IUPS2009
    • Place of Presentation
      Kyoto Japan
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2009

    • Author(s)
      S Higashijima, C Satou, Y Kimura
    • Organizer
      The 32th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Nagoya,Japan
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2009

    • Author(s)
      S Higashijima
    • Organizer
      3rd Viktor Hamburger Symposium
    • Place of Presentation
      Chicago,USA
    • Related Report
      2010 Self-evaluation Report

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Published: 2008-04-01   Modified: 2019-07-29  

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