Genetically encoded probes for imaging molecular processes in vivo

• Project Area: Systems molecular ethology to understand the operating principle of the nervous system
• Project/Area Number: 20115008
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Moritoshi 東京大学, 大学院・総合文化研究科, 准教授 (00323501)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥84,500,000 (Direct Cost: ¥65,000,000、Indirect Cost: ¥19,500,000); Fiscal Year 2012: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2011: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2010: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2009: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2008: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
• Keywords: イメージング / 蛍光 / プローブ / モデル小動物 / 神経 / 光操作 / GFP / 遺伝子コード型プローブ / 可視化 / 光制御 / 神経細胞

Research Abstract

Fluorescence imaging could be the most powerful technique available for observing spatial and temporal dynamics of molecular processes in living cells and living individuals, if fluorescent probes for the relevant cellular signaling processes become available. In the present research project, we have developed genetically encoded fluorescent indicators for cellular signaling processes, such as reactive oxygen species and cyclic nucleotides, and also developed molecular tool to manipulate cellular signaling processes with light.

Research Products

• [Journal Article] 生体脂質を可視化する蛍光プローブ (2012)
  • Author(s): 佐藤守俊
  • Journal Title: 膜 Volume: 37 Pages: 164-167
  • NAID: 10030986114
  • Peer Reviewed
• [Journal Article] Hydrogen peroxide depletes phosphatidylinosidol-3-phosphate from endosomes in a p38 MAPK-dependent manner and perturbs endocytosis. Biochim. Biophys. Acta-Mol (2011)
  • Author(s): Kano, F., Arai, T., Matsuto, M., Hayashi, H., Sato, M. and Murata, M
  • Journal Title: Cell Res Volume: 1813 Pages: 784-801
  • Peer Reviewed
• [Journal Article] Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells (2011)
  • Author(s): Oya, M., Suzuki, H., Watanabe, Y., Sato, M., and Tsuboi, T
  • Journal Title: Genes Cells Volume: 16 Pages: 608-616
  • Peer Reviewed
• [Journal Article] Superluminescent variants of marine luciferases for bioassays (2011)
  • Author(s): Kim, S.B., Suzuki, H., Sato, M., and Tao, H
  • Journal Title: Anal. Chem Volume: 83 Pages: 8732-8740
  • Peer Reviewed
• [Journal Article] Superluminescent Variants of Marine Luciferases for Bioassays (2011)
  • Author(s): S.B.Kim, H.Suzuki, M.Sato, H.Tao
  • Journal Title: Analytical Chemistry Volume: 83 Pages: 8732-8740
  • Peer Reviewed
• [Journal Article] Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells (2011)
  • Author(s): Oya, H.Suzuki, Y.Watanabe, M.Sato, T.Ts
  • Journal Title: Genes to Cells Volume: 16 Pages: 608-616
  • Peer Reviewed
• [Journal Article] Hydrogen peroxide depletes phosphatidylinosidol-3-phosphatefrom endosomes in a p38 MAPK-dependent manner and perturbs endocytosis (2011)
  • Author(s): F.Kano, T.Arai, M.Matsuto, H.Hayashi, M.Sato, M.Murata
  • Journal Title: Biochimica et Biophysica Acta Volume: 1,813 Pages: 784-801
  • Peer Reviewed
• [Journal Article] Genetically encoded fluorescent indicators to visualize protein phosphorylation by c-Jun NH_2-Terminal Kinase (JNK) in living cells (2010)
  • Author(s): Suzuki, H., and Sato, M
  • Journal Title: Supramol. Chem Volume: 22 Pages: 434-439
  • Peer Reviewed
• [Journal Article] An efficient surface modification using 2-methacryloyloxyethyl phosphorylcholine to control cell attachment via photochemical reaction in a microchannel (2010)
  • Author(s): Jang, K., Sato, K., Tanaka, Y., Yan, X., Sato, M., Nakajima, T., Mawatari, K., Konno, T., Ishihara K., and Kitamori, T
  • Journal Title: Lab Chip Volume: 10 Pages: 1937-1945
  • Peer Reviewed
• [Journal Article] Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation by c-Jun NH2-Terminal Kinase (JNK) in Living Cells (2010)
  • Author(s): H.Suzuki, M.Sato
  • Journal Title: Supramol.Chem. 22 Pages: 434-439
  • Peer Reviewed
• [Journal Article] Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphory lation by c-Jun NH_2-Terminal Kinase (JNK) in Living Cells (2010)
  • Author(s): Hideyuki Suzuki
  • Journal Title: Supramolecular Chmeistry Volume: 22 Pages: 434-439
  • Peer Reviewed
• [Journal Article] A split Gaussia luciferase-based bioluminescence template for tracing protein dynamics in living cells (2009)
  • Author(s): Kim, S.B., Sato, M., and Tao, H
  • Journal Title: Anal. Chem Volume: 81 Pages: 67-74
  • Peer Reviewed
• [Journal Article] Genetically encoded bioluminescent indicators for stress hormones (2009)
  • Author(s): Kim, S.B., Sato, M., and Tao, H
  • Journal Title: Anal. Chem Volume: 81 Pages: 3760-3768
  • Peer Reviewed
• [Journal Article] Molecular tension-indexed bioluminescent probes for determining protein-protein interactions (2009)
  • Author(s): Kim, S.B., Sato, M., and Tao, H
  • Journal Title: Bioconjugate Chem Volume: 20 Pages: 2324-2330
  • Peer Reviewed
• [Journal Article] Molecular Tension-Indexed Bioluminescent Probes for Determining Protein-Protein interactions (2009)
  • Author(s): S.B.Kim, M.Sato, H.Tao
  • Journal Title: Bioconjugate Chem. 20 Pages: 2324-2330
  • Peer Reviewed
• [Journal Article] Genetically Encoded Bioluminescent Indicators for Stress Hormones (2009)
  • Author(s): S.B.Kim, M.Sato, H.Tao
  • Journal Title: Anal.Chem. 81 Pages: 3760-3768
  • Peer Reviewed
• [Journal Article] A Split Gaussia Luciferase-Based Bioluminescence Template for Tracing Protein Dynamics in Living Cells (2009)
  • Author(s): S.B.Kim, M.Sato, H.Tao
  • Journal Title: Anal.Chem. 81 Pages: 67-74
  • Peer Reviewed
• [Journal Article] Circularly permutated bioluminescent probes for illuminating ligand-activated protein dynamics (2008)
  • Author(s): Kim, S.B., Sato, M., and Tao, H
  • Journal Title: Bioconjugate Chem Volume: 19 Pages: 2480-2486
  • Peer Reviewed
• [Journal Article] Circularly Permutated Bioluminescent Probes for Illuminating Ligand-Activated Protein Dynamics (2008)
  • Author(s): S.B.Kim, M.Sato, H.Tao
  • Journal Title: Bioconjugate Chem. 19 Pages: 2480-2486
  • Peer Reviewed
• [Journal Article] Rapidly reversible manipulation of molecular activities using dual chemical dimerizers
  • Author(s): Lin, Y.C., Nihongaki, Y., Lin, T. Y., Razavi, S., Sato, M., and Inoue, T
  • Journal Title: Angew. Chem. Int. Ed Volume: (発表予定)
  • Peer Reviewed
• [Presentation] 生命現象を見る技術・操作する技術 (2012)
  • Author(s): 佐藤守俊
  • Organizer: フロンティアシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2012-11-16
• [Presentation] 細胞内の分子過程を探索する遺伝子コード型の分子プローブ (2012)
  • Author(s): 佐藤守俊
  • Organizer: 日本化学会第61年会
  • Place of Presentation: 金沢
  • Year and Date: 2012-09-19
• [Presentation] Methods to visualize molecular processes in living cells (2012)
  • Author(s): Moritoshi Sato
  • Organizer: JASIS
  • Place of Presentation: 千葉
  • Year and Date: 2012-09-07
• [Presentation] 生体分子を可視化する技術・操作する技術 (2012)
  • Author(s): 佐藤守俊
  • Organizer: 京都大学化学研究所生体分子情報研究領域セミナー
  • Place of Presentation: 京都
  • Year and Date: 2012-06-21
• [Presentation] 細胞内シグナル伝達を可視化する技術・操作する技術 (2012)
  • Author(s): 佐藤守俊
  • Organizer: 日本膜学会第34年会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-09
• [Presentation] 生体分子を可視化する技術・操作する技術 (2011)
  • Author(s): 佐藤守俊
  • Organizer: 光操作研究会
  • Place of Presentation: 愛知
  • Year and Date: 2011-09-30
• [Presentation] 細胞内の分子を見るナノテクノロジー (2011)
  • Author(s): 佐藤守俊
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山
  • Year and Date: 2011-09-29
• [Presentation] 細胞内シグナル伝達を可視化する分子プローブ (2011)
  • Author(s): 佐藤守俊
  • Organizer: 第24回植物脂質シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-09-19
• [Presentation] 細胞内シグナル伝達を可視化する分子プローブ (2011)
  • Author(s): 佐藤守俊
  • Organizer: 日本化学会第24回関東支部大会
  • Place of Presentation: 東京
  • Year and Date: 2011-08-31
• [Presentation] "分子イメージング" 細胞内の分子を見るナノテクノロジー (2011)
  • Author(s): 佐藤守俊
  • Organizer: 第24回学際融合ビジュアライゼーションシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-01-11
• [Presentation] 細胞内のシグナル伝達を可視化する分子プローブ (2010)
  • Author(s): 佐藤守俊
  • Organizer: 日本薬学会第130年会シンポジウム「薬学における生命志向型化学(分子設計を基盤とした生命現象解明)」
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] 「薬学における生命志向型化学(分子設計を基盤とした生命現象解明)」 細胞内のシグナル伝達を可視化する分子プローブ (2010)
  • Author(s): 佐藤守俊
  • Organizer: 日本薬学会第130年会シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] Imaging molecular processes in living cells (2009)
  • Author(s): M.Sato
  • Organizer: Janelia Farm Conferecne on Fluorescent Proteins and Biological Sensors II
  • Place of Presentation: Virginia, USA
  • Year and Date: 2009-11-03
• [Presentation] 分子イメージング細胞の中の分子を見るナノテクノロジー (2009)
  • Author(s): 佐藤守俊
  • Organizer: 東京大学大学院工学系研究科応用化学専攻談話会「応用化学の新しい潮流」
  • Place of Presentation: 東京
  • Year and Date: 2009-10-03
• [Presentation] 細胞内のシグナル伝達を可視化する分子プローブ (2009)
  • Author(s): 佐藤守俊
  • Organizer: 日本分析化学会第58年会化学センサー懇談会
  • Place of Presentation: 北海道
  • Year and Date: 2009-09-24
• [Presentation] Imaging molecular processes in living cells (2009)
  • Author(s): M.Sato
  • Organizer: 2nd Swiss-Japanese Symposium on Bionanotechnology BioNano 2009
  • Place of Presentation: Aigle Castle, Switzerland
  • Year and Date: 2009-09-11
• [Presentation] Fluorescent indicators to visualize molecular processes in living cells (2009)
  • Author(s): M.Sato
  • Organizer: Cold Spring Harbor Laboratory Meeting on Single Cell Techniques
  • Place of Presentation: New York, USA
  • Year and Date: 2009-07-17
• [Presentation] 細胞内で生起する分子過程の可視化計測法 (2008)
  • Author(s): 佐藤守俊
  • Organizer: 日本バイオマテリアル学会シンポジウム2008「バイオイメージングのためのバイオマテリアル」
  • Place of Presentation: 東京
  • Year and Date: 2008-11-18
• [Remarks]
  • URL: http://satolab.c.u-tokyo.ac.jp