| Budget Amount *help |
¥171,210,000 (Direct Cost: ¥131,700,000、Indirect Cost: ¥39,510,000)
Fiscal Year 2012: ¥35,620,000 (Direct Cost: ¥27,400,000、Indirect Cost: ¥8,220,000)
Fiscal Year 2011: ¥35,620,000 (Direct Cost: ¥27,400,000、Indirect Cost: ¥8,220,000)
Fiscal Year 2010: ¥35,490,000 (Direct Cost: ¥27,300,000、Indirect Cost: ¥8,190,000)
Fiscal Year 2009: ¥35,490,000 (Direct Cost: ¥27,300,000、Indirect Cost: ¥8,190,000)
Fiscal Year 2008: ¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000)
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| Research Abstract |
We succeeded to produce mouse sperm from spermatogonial stem cells (SSCs) in vitro. We adopted an organ culture method, and found that serum replacement was effective to induce complete spermatogenesis in our system. When cultured SSCs were transplanted into the seminiferous tubules of extracted host testes, they also differentiated into sperm in the cultured testis tissues. In addition, we demonstrated that an infertile model mouse, Sl/Sl^d mutant, can be treated with the explantation of their testis tissues.
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