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Optimization and sophistication of stimulated Raman microscope for functional Raman probes

Planned Research

Project AreaInnovative multiplex imaging with functional Raman probes
Project/Area Number 20H05725
Research Category

Grant-in-Aid for Transformative Research Areas (B)

Allocation TypeSingle-year Grants
Review Section Transformative Research Areas, Section (II)
Research InstitutionThe University of Tokyo

Principal Investigator

Ozeki Yasuyuki  東京大学, 大学院工学系研究科(工学部), 教授 (60437374)

Project Period (FY) 2020-10-02 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000)
Fiscal Year 2022: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2021: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Keywordsラマンプローブ / 誘導ラマン散乱 / activatableプローブ / 超解像イメージング / 超多重イメージング / 多重イメージング
Outline of Research at the Start

生体内における様々な生体分子の複雑な相互作用を包括的に理解するには、複数種類の生体分子の空間分布や相互作用を同時に計測することが重要である。蛍光イメージング法は生体分子の標識や計測、超解像イメージングを実現する基盤技術であるが、色数が4-5色に制限される。本研究では、小関が開発を進めてきた高速・多色ラマン顕微鏡を最適化・高度化することで、本領域で創出する機能性ラマンプローブの性能を最大限に活用し、様々な多重イメージングモダリティを実証する。

Outline of Final Research Achievements

In this study, we have optimized and advanced the high-speed stimulated Raman scattering imaging system that we developed in order to achieve multiplex enzyme imaging, multiplex in vivo imaging, and multiplex-super-resolution imaging using the functional Raman probes created in this research field. Then we demonstrated the usefulness of these imaging methods through applications in biology. Specifically, we successfully demonstrated the use of activatable Raman probes created by the Kamiya team, demonstrated the photoswitchable Raman probes with commercially available photochromic molecules, demonstrated super-resolution imaging with high-performance photoswitchable Raman probes developed by Kamiya team, and demonstrated amino acid metabolic imaging with deuterium labeling in collaboration with Obata team.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、分子の電子・分子振動の光応答を周囲環境や光照射によって制御するという新しい原理を用いた生体観察手法を創出するものです。これにより、生体内の多数の構成要素を同時に観察することが可能となり、複雑な生体の仕組みを解明する新しい手法を実現できました。本研究成果は、生物学や医学の様々な分野において生命の仕組みや病気の解明の研究に活用されることが期待できます。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (27 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 5 results) Presentation (12 results) (of which Int'l Joint Research: 6 results,  Invited: 10 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] コロンビア大学/ハワイ大学(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 清華大学(中国)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 9‐Cyano‐10‐telluriumpyronin Derivatives as Red‐light‐activatable Raman Probes2023

    • Author(s)
      Kawatani Minoru、Spratt Spencer J.、Fujioka Hiroyoshi、Shou Jingwen、Misawa Yoshihiro、Kojima Ryosuke、Urano Yasuteru、Ozeki Yasuyuki、Kamiya Mako
    • Journal Title

      Chemistry An Asian Journal

      Volume: 18 Issue: 2

    • DOI

      10.1002/asia.202201086

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unprecedented Regioselective Deuterium‐Incorporation of Alkyltrimethylammonium Chlorides and Raman Analysis2023

    • Author(s)
      Sawama Yoshinari、Matsuda Takumi、Moriyama Shogo、Ban Kazuho、Fujioka Hiroyoshi、Kamiya Mako、Shou Jingwen、Ozeki Yasuyuki、Akai Shuji、Sajiki Hironao
    • Journal Title

      Asian Journal of Organic Chemistry

      Volume: 12 Issue: 3

    • DOI

      10.1002/ajoc.202200710

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering2023

    • Author(s)
      Spratt Spencer J.、Mizuguchi Takaha、Akaboshi Hikaru、Kosakamoto Hina、Okada Rina、Obata Fumiaki、Ozeki Yasuyuki
    • Journal Title

      Frontiers in Chemistry

      Volume: 11 Pages: 1141920-1141920

    • DOI

      10.3389/fchem.2023.1141920

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activatable Raman Probes Utilizing Enzyme-Induced Aggregate Formation for Selective Ex Vivo Imaging2023

    • Author(s)
      Fujioka Hiroyoshi、Kawatani Minoru、Spratt Spencer John、Komazawa Ayumi、Misawa Yoshihiro、Shou Jingwen、Mizuguchi Takaha、Kosakamoto Hina、Kojima Ryosuke、Urano Yasuteru、Obata Fumiaki、Ozeki Yasuyuki、Kamiya Mako
    • Journal Title

      Journal of the American Chemical Society

      Volume: 145 Issue: 16 Pages: 8871-8881

    • DOI

      10.1021/jacs.2c12381

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Probing Methionine Uptake in Live Cells by Deuterium Labeling and Stimulated Raman Scattering2022

    • Author(s)
      Spratt Spencer J., Oguchi Kenichi, Miura Keisuke, Asanuma Masato, Kosakamoto Hina, Obata Fumiaki, Ozeki Yasuyuki
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 126 Issue: 8 Pages: 1633-1639

    • DOI

      10.1021/acs.jpcb.1c08343

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoswitchable stimulated Raman scattering spectroscopy and microscopy2021

    • Author(s)
      Shou Jingwen, Ozeki Yasuyuki
    • Journal Title

      Optics Letters

      Volume: 46 Issue: 9 Pages: 2176-2176

    • DOI

      10.1364/ol.418240

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Super-multiplex imaging of cellular dynamics and heterogeneity by integrated stimulated Raman and fluorescence microscopy2021

    • Author(s)
      Shou Jingwen, Oda Robert, Hu Fanghao, Karasawa Keiko, Nuriya Mutsuo, Yasui Masato, Shiramizu Bruce, Min Wei, Ozeki Yasuyuki
    • Journal Title

      iScience

      Volume: 24 Issue: 8 Pages: 102832-102832

    • DOI

      10.1016/j.isci.2021.102832

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Low-intensity-noise wavelength-tunable picosecond Yb fiber laser2021

    • Author(s)
      Dai Gaoyu, Goh Chee Seong, Ozeki Yasuyuki
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 60 Issue: 8 Pages: 080902-080902

    • DOI

      10.35848/1347-4065/ac17db

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of excess intensity noise of picosecond Yb soliton fiber lasers in a >10-mW power regime2021

    • Author(s)
      G. Dai, K. Katoh, and Y. Ozeki
    • Journal Title

      Opt. Express

      Volume: 29 Issue: 8 Pages: 11702-11711

    • DOI

      10.1364/oe.422846

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities2020

    • Author(s)
      Fujioka Hiroyoshi、Shou Jingwen、Kojima Ryosuke、Urano Yasuteru、Ozeki Yasuyuki、Kamiya Mako
    • Journal Title

      Journal of the American Chemical Society

      Volume: 142 Issue: 49 Pages: 20701-20707

    • DOI

      10.1021/jacs.0c09200

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 重水素化アミノ酸によるラマン代謝イメージング2023

    • Author(s)
      小関泰之、スペンサージョンスプラット、小幡史明
    • Organizer
      第143回日本薬学会年次大会、S59-03、北海道大学、2023年3月28日。
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] High-speed multicolor stimulated Raman microscopy2022

    • Author(s)
      Y. Ozeki
    • Organizer
      International Conference on Raman Spectroscopy (ICORS2022), NEWT-1-1, Long Beach, Aug. 15, 2022.
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Probing Methionine Uptake in Live Cells and Tissue by Deuterium Labelling and Stimulated Raman Scattering2022

    • Author(s)
      Spencer John Spratt, Kenichi Oguchi, Hina Kosakamoto, Fumiaki Obata, Yasuyuki Ozeki
    • Organizer
      The 83rd JSAP Autumn Meeting, 20p-C301-11, September 20, 2022.
    • Related Report
      2022 Annual Research Report
  • [Presentation] 誘導ラマン散乱顕微法のためのパルスファイバレーザ2022

    • Author(s)
      小関泰之
    • Organizer
      レーザー学会第570回研究会「次世代ファイバレーザー技術」、名古屋大学、2022年11月18日。
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 誘導ラマン散乱顕微法による超多重イメージングおよび代謝イメージング2022

    • Author(s)
      小関泰之
    • Organizer
      日本分子生物学会、3AW-11-3、幕張メッセ、2022年12月2日。
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 誘導ラマン散乱顕微法のための低雑音/高機能パルスファイバーレーザーの開発2022

    • Author(s)
      小関泰之
    • Organizer
      レーザー学会学術講演会第43回年次大会、S06-18p-V-07、2023年1月18日。
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] High-speed multicolor stimulated Raman imaging2022

    • Author(s)
      Y. Ozeki
    • Organizer
      Photonics West on demand, Advanced Chemical Microscopy for Life Science and Translational Medicine, paper 1197305
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multicolor stimulated Raman scattering microscopy and its applications2022

    • Author(s)
      Y. Ozeki
    • Organizer
      Photonics West on demand, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, paper 1195709
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Photoswitchable stimulated Raman scattering spectroscopy and microscopy2022

    • Author(s)
      J. Shou and Y. Ozeki
    • Organizer
      Photonics West on demand, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, paper 119570A
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multicolor stimulated Raman scattering microscopy -Technologies and applications-2021

    • Author(s)
      Y. Ozeki
    • Organizer
      Sixteenth Annual Chautauqua on Nonlinear Optics, Purdue University, June 30, 2021.
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multicolor imaging with stimulated Raman scattering2021

    • Author(s)
      Y. Ozeki
    • Organizer
      International Conference on Advanced Vibrational Spectroscopy (ICAVS11), Session 1.2.2
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 「誘導ラマン散乱顕微法の発展とその応用」2021

    • Author(s)
      小関泰之
    • Organizer
      光エレクトロニクス産学連携研究専門委員会研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Book] Stimulated Raman Scattering Microscopy -Techniques and Applications-2021

    • Author(s)
      Ji-Xin Cheng, Wei Min, Yasuyuki Ozeki, Dario Polli
    • Total Pages
      610
    • Publisher
      Elsevier
    • ISBN
      9780323851589
    • Related Report
      2021 Annual Research Report
  • [Remarks] Ozeki Group

    • URL

      https://sites.google.com/site/ozekibp/

    • Related Report
      2022 Annual Research Report
  • [Remarks] Yasuyuki Ozeki's Personal Website

    • URL

      https://sites.google.com/site/ysozeki/

    • Related Report
      2022 Annual Research Report

URL: 

Published: 2020-10-30   Modified: 2024-01-30  

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