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Whole-brain imaging in single-cell resolution visualizes Glia function in sleep-wake cycle

Planned Research

Project AreaGlia decoding: deciphering information critical for brain-body interactions
Project/Area Number 20H05903
Research Category

Grant-in-Aid for Transformative Research Areas (A)

Allocation TypeSingle-year Grants
Review Section Transformative Research Areas, Section (III)
Research InstitutionUniversity of Tsukuba (2021-2024)
The University of Tokyo (2020)

Principal Investigator

史 蕭逸  筑波大学, 国際統合睡眠医科学研究機構, 助教 (40803656)

Co-Investigator(Kenkyū-buntansha) 松本 桂彦  東京大学, 大学院医学系研究科(医学部), 講師 (60632859)
戸根 大輔  東京大学, 大学院医学系研究科(医学部), 助教 (60806610)
Project Period (FY) 2020-11-19 – 2025-03-31
Project Status Granted (Fiscal Year 2024)
Budget Amount *help
¥103,220,000 (Direct Cost: ¥79,400,000、Indirect Cost: ¥23,820,000)
Fiscal Year 2024: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2023: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2022: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2021: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2020: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Keywords睡眠 / グリア細胞 / 1細胞解析 / FISH / グリア / 透明化 / CUBIC / イメージング / 全脳透明化
Outline of Research at the Start

全脳1細胞解像度のグリア細胞解析技術を開発し、睡眠覚醒サイクルにおけるグリア細胞の役割を明らかにする。

Outline of Annual Research Achievements

マウスにおける全脳1細胞レベルのイメージング技術をグリア細胞に応用した。
具体的には、B6Nマウスの脳に対して、透明化処理および、Iba1、GFAP-A647、Olig2、SYTOX-Gの染色プロトコールの開発を行い、グリア細胞における主要細胞の染色とデータ解析を可能にした。また、in situ hybridization (FISH) 法による細胞の可視化解析技術の開発を進めており、その結果を学会発表および論文として部分的に公開している。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

予定していたグリア細胞のイメージング技術の開発は概ね順調に進展している。睡眠とグリア細胞の関係性の可視化に関しても現在、精力的に進めており、スケジュール通りの進捗と言える。さらに、染色技術としてFISHの開発も順調に進んでおり、抗体染色との併用に成功している。

Strategy for Future Research Activity

今後は、FISH法に関してさらに開発を進めるとともに、論文執筆に向けてデータ解析を中心に進める。またに睡眠覚醒の表現型とグリア細胞の関係性に関して進める。

Report

(3 results)
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (22 results)

All 2023 2022 2021 2020

All Journal Article (19 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 19 results,  Open Access: 19 results) Presentation (2 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] SIK3-HDAC4 in the suprachiasmatic nucleus regulates the timing of arousal at the dark onset and circadian period in mice2023

    • Author(s)
      Asano Fuyuki、Kim Staci J.、Fujiyama Tomoyuki、Miyoshi Chika、Hotta-Hirashima Noriko、Asama Nodoka、Iwasaki Kanako、Kakizaki Miyo、Mizuno Seiya、Mieda Michihiro、Sugiyama Fumihiro、Takahashi Satoru、Shi Shoi、Hirano Arisa、Funato Hiromasa、Yanagisawa Masashi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 120 Issue: 11 Pages: 1-11

    • DOI

      10.1073/pnas.2218209120

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Distinct phosphorylation states of mammalian CaMKIIβ control the induction and maintenance of sleep2022

    • Author(s)
      Daisuke Tone, Koji L Ode, Qianhui Zhang, Hiroshi Fujishima, Rikuhiro G Yamada, et al.
    • Journal Title

      PLOS Biology

      Volume: 20 Issue: 10 Pages: 3001813-3001813

    • DOI

      10.1371/journal.pbio.3001813

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimating Infection-Related Human Mobility Networks Based on Time Series Data of COVID-19 Infection in Japan2022

    • Author(s)
      Yamada Tetsuya、Shi Shoi
    • Journal Title

      Applied Sciences

      Volume: 12 Issue: 18 Pages: 9236-9236

    • DOI

      10.3390/app12189236

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A design principle of spindle oscillations in mammalian sleep2022

    • Author(s)
      Yamada Tetsuya、Shi Shoi、Ueda Hiroki R.
    • Journal Title

      iScience

      Volume: 25 Issue: 3 Pages: 103873-103873

    • DOI

      10.1016/j.isci.2022.103873

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The 103,200-arm acceleration dataset in the UK Biobank revealed a landscape of human sleep phenotypes2022

    • Author(s)
      Katori Machiko、Shi Shoi、Ode Koji L.、Tomita Yasuhiro、Ueda Hiroki R.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 12

    • DOI

      10.1073/pnas.2116729119

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A jerk-based algorithm ACCEL for the accurate classification of sleep?wake states from arm acceleration2022

    • Author(s)
      Ode Koji L.、Shi Shoi、Katori Machiko、Mitsui Kentaro、Takanashi Shin、Oguchi Ryo、Aoki Daisuke、Ueda Hiroki R.
    • Journal Title

      iScience

      Volume: 25 Issue: 2 Pages: 103727-103727

    • DOI

      10.1016/j.isci.2021.103727

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole-organ analysis of TGF-β-mediated remodelling of the tumour microenvironment by tissue clearing.2021

    • Author(s)
      Kubota SI, Takahashi K, Mano T, Matsumoto K, Katsumata T, Shi S, Tainaka K, Ueda HR, Ehata S, Miyazono K.
    • Journal Title

      Commun. Biol.

      Volume: 4 Issue: 1 Pages: 294-294

    • DOI

      10.1038/s42003-021-01786-y

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CUBIC-Cloud provides an integrative computational framework toward community-driven whole-mouse-brain mapping2021

    • Author(s)
      Mano T, Murata K, Kon K, Shimizu C, Ono H, Shi S, Yamada RG, Miyamichi K, Susaki EA, Touhara K, Ueda HR.
    • Journal Title

      Cell Reports Methods

      Volume: 1 Issue: 2 Pages: 100038-100038

    • DOI

      10.1016/j.crmeth.2021.100038

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Trends in Healthcare Access in Japan during the First Wave of the COVID-19 Pandemic, up to June 20202021

    • Author(s)
      Makiyama Koji、Kawashima Takayuki、Nomura Shuhei、Eguchi Akifumi、Yoneoka Daisuke、Tanoue Yuta、Kawamura Yumi、Sakamoto Haruka、Gilmour Stuart、Shi Shoi、Matsuura Kentaro、Uryu Shinya、Hashizume Masahiro
    • Journal Title

      International Journal of Environmental Research and Public Health

      Volume: 18 Issue: 6 Pages: 3271-3271

    • DOI

      10.3390/ijerph18063271

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impact of domestic travel restrictions on transmission of COVID-19 infection using public transportation network approach2021

    • Author(s)
      Murano Yayoi、Ueno Ryo、Shi Shoi、Kawashima Takayuki、Tanoue Yuta、Tanaka Shiori、Nomura Shuhei、Shoji Hiromichi、Shimizu Toshiaki、Nguyen Huy、Miyata Hiroaki、Gilmour Stuart、Yoneoka Daisuke
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1

    • DOI

      10.1038/s41598-021-81806-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assessing the regional impact of the Japan’s COVID-19 state of emergency declaration: a population-level observational study using social networking services2021

    • Author(s)
      Yoneoka D, Shi S, Nomura S, Tanoue Y, Kawashima T, Eguchi A, Matsuura K, Makiyama K, Uryu S, Ejima K, Sakamoto H, Taniguchi T, Kunishima H, Gilmour S, Nishiura H, Miyata H.
    • Journal Title

      BMJ Open

      Volume: 11 Issue: 2 Pages: e042002-e042002

    • DOI

      10.1136/bmjopen-2020-042002

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Excess All-Cause Deaths during Coronavirus Disease Pandemic, Japan, January?May 202012021

    • Author(s)
      Kawashima Takayuki、Nomura Shuhei、Tanoue Yuta、Yoneoka Daisuke、Eguchi Akifumi、Ng Chris Fook Sheng、Matsuura Kentaro、Shi Shoi、Makiyama Koji、Uryu Shinya、Kawamura Yumi、Takayanagi Shinichi、Gilmour Stuart、Miyata Hiroaki、Sunagawa Tomimasa、Takahashi Takuri、Tsuchihashi Yuuki、
    • Journal Title

      Emerging Infectious Diseases

      Volume: 27 Issue: 3 Pages: 789-795

    • DOI

      10.3201/eid2703.203925

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The relationship between fever rate and telework implementation as a social distancing measure against the COVID-19 pandemic in Japan2021

    • Author(s)
      Kawashima T.、Nomura S.、Tanoue Y.、Yoneoka D.、Eguchi A.、Shi S.、Miyata H.
    • Journal Title

      Public Health

      Volume: 192 Pages: 12-14

    • DOI

      10.1016/j.puhe.2020.05.018

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Trend change of transmission route of COVID-19 related symptom in Japan2020

    • Author(s)
      Eguchi A, Yoneoka D, Shi S, Tanoue Y, Kawashima T, Nomura S, Matsuura K, Makiyama K, Ejima K, Gilmour S, Nishiura H, Miyata H.
    • Journal Title

      Public Health

      Volume: 187 Pages: 157-160

    • DOI

      10.1016/j.puhe.2020.08.020

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A large-scale epidemiological monitoring of the COVID-19 in Tokyo2020

    • Author(s)
      Yoneoka D, Tanoue Y, Kawashima T, Nomura S, Shi S, Eguchi A, Ejima K, Taniguchi T, Sakamoto H, Kunishima H, Gilmour S, Nishiura H, Miyata H.
    • Journal Title

      The Lancet Regional Health Western Pacific

      Volume: 3 Pages: 100016-100016

    • DOI

      10.1016/j.lanwpc.2020.100016

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Early SNS-Based Monitoring System for the COVID-19 Outbreak in Japan: A Population-Level Observational Study2020

    • Author(s)
      Yoneoka Daisuke、Kawashima Takayuki、Tanoue Yuta、Nomura Shuhei、Ejima Keisuke、Shi Shoi、Eguchi Akifumi、Taniguchi Toshibumi、Sakamoto Haruka、Kunishima Hiroyuki、Gilmour Stuart、Nishiura Hiroshi、Miyata Hiroaki
    • Journal Title

      Journal of Epidemiology

      Volume: 30 Issue: 8 Pages: 362-370

    • DOI

      10.2188/jea.je20200150

    • NAID

      130007884635

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impact of travel restrictions on importation of novel coronavirus infection: An effective distance approach2020

    • Author(s)
      Shi S, Tanaka S, Ueno R,Gilmour S, Tanoue Y, Kawashima T, Nomura S, Miyata H, Yoneoka D.
    • Journal Title

      Bull World Health Organ.

      Volume: 98 Issue: 8 Pages: 518-529

    • DOI

      10.2471/blt.20.255679

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Time to Reconsider Diverse Ways of Working in Japan to Promote Social Distancing Measures against the COVID-192020

    • Author(s)
      Nomura Shuhei、Yoneoka Daisuke、Tanoue Yuta、Kawashima Takayuki、Shi Shoi、Eguchi Akifumi、Miyata Hiroaki
    • Journal Title

      Journal of Urban Health

      Volume: 97 Issue: 4 Pages: 457-460

    • DOI

      10.1007/s11524-020-00464-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mental health of family, friends, and co-workers of COVID-19 patients in Japan2020

    • Author(s)
      Tanoue Yuta、Nomura Shuhei、Yoneoka Daisuke、Kawashima Takayuki、Eguchi Akifumi、Shi Shoi、Harada Nahoko、Miyata Hiroaki
    • Journal Title

      Psychiatry Research

      Volume: 291 Pages: 113067-113067

    • DOI

      10.1016/j.psychres.2020.113067

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Big data analysis revealed a landscape of human sleep phenotypes2022

    • Author(s)
      Shoi Shi
    • Organizer
      NEURO2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ca2+-dependent/independent hyperpolarization pathways in sleep regulation2021

    • Author(s)
      Shoi Shi
    • Organizer
      Congress of Asian Society of Sleep Medicine(
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] ダイテル Pythonプログラミング2021

    • Author(s)
      P. Deitel、H. Deitel、史 蕭逸、米岡 大輔、本田 志温
    • Total Pages
      560
    • Publisher
      東京化学同人
    • ISBN
      4807920022
    • Related Report
      2020 Annual Research Report

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Published: 2020-11-24   Modified: 2024-12-25  

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