Whole-brain imaging in single-cell resolution visualizes Glia function in sleep-wake cycle

• Project Area: Glia decoding: deciphering information critical for brain-body interactions
• Project/Area Number: 20H05903
• Research Category: Grant-in-Aid for Transformative Research Areas (A)
• Allocation Type: Single-year Grants
• Review Section: Transformative Research Areas, Section (III)
• Research Institution: University of Tsukuba (2021-2024); The University of Tokyo (2020)
• Principal Investigator: 史 蕭逸 筑波大学, 国際統合睡眠医科学研究機構, 助教 (40803656)
• Co-Investigator(Kenkyū-buntansha): 松本 桂彦 東京大学, 大学院医学系研究科(医学部), 講師 (60632859) ; 戸根 大輔 東京大学, 大学院医学系研究科(医学部), 助教 (60806610)
• Project Period (FY): 2020-11-19 – 2025-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥103,220,000 (Direct Cost: ¥79,400,000、Indirect Cost: ¥23,820,000); Fiscal Year 2024: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2023: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2022: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2021: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2020: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
• Keywords: 睡眠 / グリア細胞 / 1細胞解析 / FISH / グリア / 透明化 / CUBIC / イメージング / 全脳透明化

Outline of Research at the Start

全脳1細胞解像度のグリア細胞解析技術を開発し、睡眠覚醒サイクルにおけるグリア細胞の役割を明らかにする。

Outline of Annual Research Achievements

マウスにおける全脳１細胞レベルのイメージング技術をグリア細胞に応用した。
具体的には、B6Nマウスの脳に対して、透明化処理および、Iba1、GFAP-A647、Olig2、SYTOX-Gの染色プロトコールの開発を行い、グリア細胞における主要細胞の染色とデータ解析を可能にした。また、in situ hybridization (FISH) 法による細胞の可視化解析技術の開発を進めており、その結果を学会発表および論文として部分的に公開している。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

予定していたグリア細胞のイメージング技術の開発は概ね順調に進展している。睡眠とグリア細胞の関係性の可視化に関しても現在、精力的に進めており、スケジュール通りの進捗と言える。さらに、染色技術としてFISHの開発も順調に進んでおり、抗体染色との併用に成功している。

Strategy for Future Research Activity

今後は、FISH法に関してさらに開発を進めるとともに、論文執筆に向けてデータ解析を中心に進める。またに睡眠覚醒の表現型とグリア細胞の関係性に関して進める。

Research Products

• [Journal Article] SIK3-HDAC4 in the suprachiasmatic nucleus regulates the timing of arousal at the dark onset and circadian period in mice (2023)
  • Author(s): Asano Fuyuki、Kim Staci J.、Fujiyama Tomoyuki、Miyoshi Chika、Hotta-Hirashima Noriko、Asama Nodoka、Iwasaki Kanako、Kakizaki Miyo、Mizuno Seiya、Mieda Michihiro、Sugiyama Fumihiro、Takahashi Satoru、Shi Shoi、Hirano Arisa、Funato Hiromasa、Yanagisawa Masashi
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 120 Issue: 11 Pages: 1-11
  • DOI: 10.1073/pnas.2218209120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct phosphorylation states of mammalian CaMKIIβ control the induction and maintenance of sleep (2022)
  • Author(s): Daisuke Tone, Koji L Ode, Qianhui Zhang, Hiroshi Fujishima, Rikuhiro G Yamada, et al.
  • Journal Title: PLOS Biology Volume: 20 Issue: 10 Pages: 3001813-3001813
  • DOI: 10.1371/journal.pbio.3001813
  • Peer Reviewed / Open Access
• [Journal Article] Estimating Infection-Related Human Mobility Networks Based on Time Series Data of COVID-19 Infection in Japan (2022)
  • Author(s): Yamada Tetsuya、Shi Shoi
  • Journal Title: Applied Sciences Volume: 12 Issue: 18 Pages: 9236-9236
  • DOI: 10.3390/app12189236
  • Peer Reviewed / Open Access
• [Journal Article] A design principle of spindle oscillations in mammalian sleep (2022)
  • Author(s): Yamada Tetsuya、Shi Shoi、Ueda Hiroki R.
  • Journal Title: iScience Volume: 25 Issue: 3 Pages: 103873-103873
  • DOI: 10.1016/j.isci.2022.103873
  • Peer Reviewed / Open Access
• [Journal Article] The 103,200-arm acceleration dataset in the UK Biobank revealed a landscape of human sleep phenotypes (2022)
  • Author(s): Katori Machiko、Shi Shoi、Ode Koji L.、Tomita Yasuhiro、Ueda Hiroki R.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 119 Issue: 12
  • DOI: 10.1073/pnas.2116729119
  • Peer Reviewed / Open Access
• [Journal Article] A jerk-based algorithm ACCEL for the accurate classification of sleep?wake states from arm acceleration (2022)
  • Author(s): Ode Koji L.、Shi Shoi、Katori Machiko、Mitsui Kentaro、Takanashi Shin、Oguchi Ryo、Aoki Daisuke、Ueda Hiroki R.
  • Journal Title: iScience Volume: 25 Issue: 2 Pages: 103727-103727
  • DOI: 10.1016/j.isci.2021.103727
  • Peer Reviewed / Open Access
• [Journal Article] Whole-organ analysis of TGF-β-mediated remodelling of the tumour microenvironment by tissue clearing. (2021)
  • Author(s): Kubota SI, Takahashi K, Mano T, Matsumoto K, Katsumata T, Shi S, Tainaka K, Ueda HR, Ehata S, Miyazono K.
  • Journal Title: Commun. Biol. Volume: 4 Issue: 1 Pages: 294-294
  • DOI: 10.1038/s42003-021-01786-y
  • Peer Reviewed / Open Access
• [Journal Article] CUBIC-Cloud provides an integrative computational framework toward community-driven whole-mouse-brain mapping (2021)
  • Author(s): Mano T, Murata K, Kon K, Shimizu C, Ono H, Shi S, Yamada RG, Miyamichi K, Susaki EA, Touhara K, Ueda HR.
  • Journal Title: Cell Reports Methods Volume: 1 Issue: 2 Pages: 100038-100038
  • DOI: 10.1016/j.crmeth.2021.100038
  • Peer Reviewed / Open Access
• [Journal Article] Trends in Healthcare Access in Japan during the First Wave of the COVID-19 Pandemic, up to June 2020 (2021)
  • Author(s): Makiyama Koji、Kawashima Takayuki、Nomura Shuhei、Eguchi Akifumi、Yoneoka Daisuke、Tanoue Yuta、Kawamura Yumi、Sakamoto Haruka、Gilmour Stuart、Shi Shoi、Matsuura Kentaro、Uryu Shinya、Hashizume Masahiro
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 18 Issue: 6 Pages: 3271-3271
  • DOI: 10.3390/ijerph18063271
  • Peer Reviewed / Open Access
• [Journal Article] Impact of domestic travel restrictions on transmission of COVID-19 infection using public transportation network approach (2021)
  • Author(s): Murano Yayoi、Ueno Ryo、Shi Shoi、Kawashima Takayuki、Tanoue Yuta、Tanaka Shiori、Nomura Shuhei、Shoji Hiromichi、Shimizu Toshiaki、Nguyen Huy、Miyata Hiroaki、Gilmour Stuart、Yoneoka Daisuke
  • Journal Title: Scientific Reports Volume: 11 Issue: 1
  • DOI: 10.1038/s41598-021-81806-3
  • Peer Reviewed / Open Access
• [Journal Article] Assessing the regional impact of the Japan’s COVID-19 state of emergency declaration: a population-level observational study using social networking services (2021)
  • Author(s): Yoneoka D, Shi S, Nomura S, Tanoue Y, Kawashima T, Eguchi A, Matsuura K, Makiyama K, Uryu S, Ejima K, Sakamoto H, Taniguchi T, Kunishima H, Gilmour S, Nishiura H, Miyata H.
  • Journal Title: BMJ Open Volume: 11 Issue: 2 Pages: e042002-e042002
  • DOI: 10.1136/bmjopen-2020-042002
  • Peer Reviewed / Open Access
• [Journal Article] Excess All-Cause Deaths during Coronavirus Disease Pandemic, Japan, January?May 20201 (2021)
  • Author(s): Kawashima Takayuki、Nomura Shuhei、Tanoue Yuta、Yoneoka Daisuke、Eguchi Akifumi、Ng Chris Fook Sheng、Matsuura Kentaro、Shi Shoi、Makiyama Koji、Uryu Shinya、Kawamura Yumi、Takayanagi Shinichi、Gilmour Stuart、Miyata Hiroaki、Sunagawa Tomimasa、Takahashi Takuri、Tsuchihashi Yuuki、
  • Journal Title: Emerging Infectious Diseases Volume: 27 Issue: 3 Pages: 789-795
  • DOI: 10.3201/eid2703.203925
  • Peer Reviewed / Open Access
• [Journal Article] The relationship between fever rate and telework implementation as a social distancing measure against the COVID-19 pandemic in Japan (2021)
  • Author(s): Kawashima T.、Nomura S.、Tanoue Y.、Yoneoka D.、Eguchi A.、Shi S.、Miyata H.
  • Journal Title: Public Health Volume: 192 Pages: 12-14
  • DOI: 10.1016/j.puhe.2020.05.018
  • Peer Reviewed / Open Access
• [Journal Article] Trend change of transmission route of COVID-19 related symptom in Japan (2020)
  • Author(s): Eguchi A, Yoneoka D, Shi S, Tanoue Y, Kawashima T, Nomura S, Matsuura K, Makiyama K, Ejima K, Gilmour S, Nishiura H, Miyata H.
  • Journal Title: Public Health Volume: 187 Pages: 157-160
  • DOI: 10.1016/j.puhe.2020.08.020
  • Peer Reviewed / Open Access
• [Journal Article] A large-scale epidemiological monitoring of the COVID-19 in Tokyo (2020)
  • Author(s): Yoneoka D, Tanoue Y, Kawashima T, Nomura S, Shi S, Eguchi A, Ejima K, Taniguchi T, Sakamoto H, Kunishima H, Gilmour S, Nishiura H, Miyata H.
  • Journal Title: The Lancet Regional Health Western Pacific Volume: 3 Pages: 100016-100016
  • DOI: 10.1016/j.lanwpc.2020.100016
  • Peer Reviewed / Open Access
• [Journal Article] Early SNS-Based Monitoring System for the COVID-19 Outbreak in Japan: A Population-Level Observational Study (2020)
  • Author(s): Yoneoka Daisuke、Kawashima Takayuki、Tanoue Yuta、Nomura Shuhei、Ejima Keisuke、Shi Shoi、Eguchi Akifumi、Taniguchi Toshibumi、Sakamoto Haruka、Kunishima Hiroyuki、Gilmour Stuart、Nishiura Hiroshi、Miyata Hiroaki
  • Journal Title: Journal of Epidemiology Volume: 30 Issue: 8 Pages: 362-370
  • DOI: 10.2188/jea.je20200150
  • NAID: 130007884635
  • Peer Reviewed / Open Access
• [Journal Article] Impact of travel restrictions on importation of novel coronavirus infection: An effective distance approach (2020)
  • Author(s): Shi S, Tanaka S, Ueno R,Gilmour S, Tanoue Y, Kawashima T, Nomura S, Miyata H, Yoneoka D.
  • Journal Title: Bull World Health Organ. Volume: 98 Issue: 8 Pages: 518-529
  • DOI: 10.2471/blt.20.255679
  • Peer Reviewed / Open Access
• [Journal Article] Time to Reconsider Diverse Ways of Working in Japan to Promote Social Distancing Measures against the COVID-19 (2020)
  • Author(s): Nomura Shuhei、Yoneoka Daisuke、Tanoue Yuta、Kawashima Takayuki、Shi Shoi、Eguchi Akifumi、Miyata Hiroaki
  • Journal Title: Journal of Urban Health Volume: 97 Issue: 4 Pages: 457-460
  • DOI: 10.1007/s11524-020-00464-4
  • Peer Reviewed / Open Access
• [Journal Article] Mental health of family, friends, and co-workers of COVID-19 patients in Japan (2020)
  • Author(s): Tanoue Yuta、Nomura Shuhei、Yoneoka Daisuke、Kawashima Takayuki、Eguchi Akifumi、Shi Shoi、Harada Nahoko、Miyata Hiroaki
  • Journal Title: Psychiatry Research Volume: 291 Pages: 113067-113067
  • DOI: 10.1016/j.psychres.2020.113067
  • Peer Reviewed / Open Access
• [Presentation] Big data analysis revealed a landscape of human sleep phenotypes (2022)
  • Author(s): Shoi Shi
  • Organizer: NEURO2022
  • Int'l Joint Research / Invited
• [Presentation] Ca2+-dependent/independent hyperpolarization pathways in sleep regulation (2021)
  • Author(s): Shoi Shi
  • Organizer: Congress of Asian Society of Sleep Medicine（
  • Int'l Joint Research / Invited
• [Book] ダイテル Pythonプログラミング (2021)
  • Author(s): P. Deitel、H. Deitel、史 蕭逸、米岡 大輔、本田 志温
  • Total Pages: 560
  • Publisher: 東京化学同人
  • ISBN: 4807920022