| Budget Amount *help |
¥145,080,000 (Direct Cost: ¥111,600,000、Indirect Cost: ¥33,480,000)
Fiscal Year 2024: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2023: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2022: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2021: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2020: ¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
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| Outline of Final Research Achievements |
In this study, we established a new proteogenomics platform for comprehensive and quantitative detection and analysis of unknown translation products. By extending our original iMPAQT method and combining it with DIA, we established a system capable of determining protein abundance on a large scale with high precision. Furthermore, they established N-terminal proteomics technology using multiple enzymes and identified more than 10,000 N-termini, including known and unknown, of which more than 1,900 were derived from novel ORFs. Furthermore, to comprehensively characterize the properties and functions of a large number of proteins, including unknown proteins, we have established a platform to combine multiple proteomic information such as localization, post-translational modifications, complex formation, and turnover.
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