| Budget Amount *help |
¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2009: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Outline of Final Research Achievements |
We have investigated the synthesis of various glycans from both animals and microbes in order to investigate their biological functions. The former targets are the N-glycans containing core fucose, bisecting glucosamine or bis-sialylated motif. The key issues for the synthesis were stereoselective glycosylation reactions and we developed highly efficient methods by using microfluidic conditions. We then achieved the solid-phase synthesis of sialylated N-glycan.
Microbial glycoconjugates such as partial structures of lipopolysaccharide (LPS) and peptidoglycan (PGN) from bacteria as well as inositol phospholipids from Entamoeba histolytica were also synthesized. For the synthesis of E. histolytica phospholipids, we developed new synthetic methods including regioselective phosphorylation and synthesis of the long-chain fatty acids by Ni catalyzed sp3-sp3 cross coupling reaction. We also adopted a new protecting group strategy utilizing allyl group as the permanent protecting group.
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