| Budget Amount *help |
¥114,790,000 (Direct Cost: ¥88,300,000、Indirect Cost: ¥26,490,000)
Fiscal Year 2013: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2011: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Fiscal Year 2010: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2009: ¥30,160,000 (Direct Cost: ¥23,200,000、Indirect Cost: ¥6,960,000)
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| Research Abstract |
This study describes the authentication mechanism of fertilizing sperm during migration through the female reproductive tract. We have successfully identified a sperm ADAM3-interacting membrane protein localized on epithelial cells in the uterus and oviduct. Functional analysis of the membrane protein suggests a possible involvement in release of sperm from the epithelial cells. We also found that double-knockout mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, although the mutant epididymal sperm fail to undergo the acrosome reaction on the zona surface and to traverse the zona pellucida in vitro. Moreover, We have identified a novel proteasome subunit specifically present in sperm. The function of the mouse sperm proteasome may be distinguished from sea urchin and starfish proteasomes by functional diversification.
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