| Budget Amount *help |
¥110,760,000 (Direct Cost: ¥85,200,000、Indirect Cost: ¥25,560,000)
Fiscal Year 2013: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2012: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2011: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2010: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2009: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
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| Outline of Final Research Achievements |
Small interfering RNA (siRNA) or microRNA (miRNA), approximately 20 nucleotides in length, directs the posttranscriptional repression of protein-coding genes in metazoans. In human cells, more than 1,500 miRNAs have been identified and predicted to regulate the activity of a great number of protein-coding genes to control many developmental and cellular processes, such as proliferation, apoptosis, differentiation, and neural functions; they are dysregulated in tumors and function as tumor suppressors or oncogenes. In the present study, we revealed the functional machinery of small RNAs by determining small RNA-mediated silencing efficacy using thermodynamics of base-pairing or by analyzing interacting mechanism of RNA binding proteins with small RNAs.
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