| Budget Amount *help |
¥232,440,000 (Direct Cost: ¥178,800,000、Indirect Cost: ¥53,640,000)
Fiscal Year 2013: ¥38,480,000 (Direct Cost: ¥29,600,000、Indirect Cost: ¥8,880,000)
Fiscal Year 2012: ¥38,480,000 (Direct Cost: ¥29,600,000、Indirect Cost: ¥8,880,000)
Fiscal Year 2011: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2010: ¥48,620,000 (Direct Cost: ¥37,400,000、Indirect Cost: ¥11,220,000)
Fiscal Year 2009: ¥66,300,000 (Direct Cost: ¥51,000,000、Indirect Cost: ¥15,300,000)
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| Research Abstract |
The epiblast in mouse embryos acquires proximo-distal (P-D) and antero-posterior (A-P) axes by interacting with the extra-embryonic ectoderm and visceral endoderm (VE). In the VE, down-regulation of BMP signaling required for the P-D specification occurs via mVam2-dependent microautophagy. After establishment of the A-P axis, Wnt3 in the posterior epiblast drives the primitive streak formation. We showed that epiblast stem cells (EpiSCs), which often differentiate into the mesendoderm, could be maintained by suppressing Wnt canonical pathway, showing that the Wnt signaling promotes differentiation of EpiSCs into mesendoderm. During the left-right (L-R) specification, Wnt3 was found to be expressed asymmetrically along the L-R axis in the node. We identified the molecular network involved in the asymmetric expression thereby revealed the canonical Wnt signaling as a novel mechanism for the L-R axis determination.
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