定量的超硫黄オミックス・イメージング技術の開発と標準化プロトコールの確立

• Project Area: Life Science Innovation Driven by Supersulfide Biology
• Project/Area Number: 21H05263
• Research Category: Grant-in-Aid for Transformative Research Areas (A)
• Allocation Type: Single-year Grants
• Review Section: Transformative Research Areas, Section (III)
• Research Institution: Tohoku University
• Principal Investigator: 赤池 孝章 東北大学, 医学系研究科, 教授 (20231798)
• Co-Investigator(Kenkyū-buntansha): 高田 剛 東北大学, 医学系研究科, 大学院非常勤講師 (20733257) ; 居原 秀 大阪公立大学, 大学院理学研究科, 教授 (60254447)
• Project Period (FY): 2021-09-10 – 2026-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥74,750,000 (Direct Cost: ¥57,500,000、Indirect Cost: ¥17,250,000); Fiscal Year 2025: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2024: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2023: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2022: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2021: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
• Keywords: 超硫黄分子 / 超硫黄オミックス / 超硫黄イメージング / 国際標準化

Outline of Research at the Start

超硫黄の生物学的意義を解明するには、システインパースルフィドなどの低分子超硫黄や、タンパク質中の超硫黄を定量的に解析する方法の確立が不可欠である。低分子超硫黄の定量的解析法はほぼ確立しつつあるが、タンパク質の超硫黄定量解析やプロテオーム、さらに、超硫黄分子イメージングにおいては、超硫黄側鎖の構造や機能解明の視点から、さらなる精度改良と世界標準化が望まれる。本研究では新規に開発した超硫黄プローブを用いて、定量的硫黄メタボローム・プロテオームのみならず、超硫黄オミックスイメージングを開発する。本領域で独自に確立する定量的超硫黄オミックスのプロトコールを国際標準化して国内外の超硫黄生物学を先導する。

Outline of Annual Research Achievements

本研究では、定量的超硫黄オミックスおよびイメージング技術の開発と世界標準化プロトコールとデータベースを構築する。この分析技術を基に、新たな疾病、老化制御・長寿医療の研究基盤を確立する。例えば、超硫黄オミックスやイメージングにより生体の硫黄代謝動態をモニタリングすることで、日常的な健康管理のみならず、動脈硬化などの生活習慣病、慢性炎症性肺疾患、心筋梗塞・心不全などの難治性心疾患などにおける病状把握や病態解明や予防・治療法の開発に繋げる。さらに、本超硫黄オミックス解析設備と技術を当該研究領域のバーチャルハブとして運用することで、総括班のミッションである領域内での横断的連携の強化のみならず、総括班にて先導する超硫黄のグローバルコンソーシアム構想と国際標準化・データベース化を支援し着実に遂行する。このことにより超硫黄生物学を世界的レベルで展開する。これまでに、超硫黄分子を安定的に捕捉するアルキル化プローブを用いた定量的超硫黄オミックス解析法を開発することにより、哺乳類・ヒトの細胞内に超硫黄分子が豊富に生成されることを見出した。加えて、単離ミトコンドリアを用いたシングル（単一）ミトコンドリアイメージングシステムを構築し、超硫黄分子を介したミトコンドリア膜電位形成を精度良く解析することで、真核生物における硫黄呼吸が、酸素呼吸とのハイブリット型システムであることを明らかにした。本年度では、環状八硫黄S8を特異的に捕捉する分子カプセルを用いた解析により、広く自然界に存在する環化S8が、生体内に高濃度で生成・蓄積していることを見出した。さらに、環化S8は、ミトコンドリアの硫黄依存型エネルギー代謝に関与する一方で、脂質過酸化を強力に制御することを明らかにした。引き続き、定量的な超硫黄分析技術を基にした超硫黄生物学研究を国際的に強力に推進する。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

超硫黄分子の定量的オミックス・イメージング技術開発と標準化プロトコールの確立に向けた解析を行ったが、そのために本研究の当初目的の一つである、環化S8の定量解析法の開発とその生理機能解明に関する知見をさらに深めることができ、研究はおおむね順調に進展していると評価できる。

Strategy for Future Research Activity

本研究の当初目的に照らして、研究遂行の上での問題はなく順調に研究が進展している。今後は、定量的硫黄メタボローム・プロテオームのみならず、超硫黄オミックスイメージングの開発を推進する。さらに、本領域で独自に確立する定量的超硫黄オミックスのプロトコールを国際標準化して国内外の超硫黄生物学を先導する。

Research Products

• [Int'l Joint Research] German Cancer Research Center/Helmholtz(ドイツ)
• [Int'l Joint Research] National Institute of Oncology(ハンガリー)
• [Int'l Joint Research] University of Vermont/Brown University(米国)
• [Int'l Joint Research] Birmingham(英国)
• [Journal Article] 2H-Thiopyran-2-thione sulfine, a compound for converting H2S to HSOH/H2S2 and increasing intracellular sulfane sulfur levels (2024)
  • Author(s): Cui Qi、Shieh Meg、Pan Tony W.、Nishimura Akiyuki、Matsunaga Tetsuro、Kelly Shane S.、Xu Shi、Jung Minkyung、Ogata Seiryo、Morita Masanobu、Yoshitake Jun、Chen Xiaoyan、Robinson Jerome R.、Qian Wei-Jun、Nishida Motohiro、Akaike Takaaki、Xian Ming
  • Journal Title: Nature Communications Volume: 15 Issue: 1 Pages: 2453-2453
  • DOI: 10.1038/s41467-024-46652-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Essential role of ROS - 8-Nitro-cGMP signaling in long-term memory of motor learning and cerebellar synaptic plasticity (2024)
  • Author(s): Kakizawa Sho、Arasaki Tomoko、Yoshida Ayano、Sato Ayami、Takino Yuka、Ishigami Akihito、Akaike Takaaki、Yanai Shuichi、Endo Shogo
  • Journal Title: Redox Biology Volume: 70 Pages: 103053-103053
  • DOI: 10.1016/j.redox.2024.103053
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of innate immune and inflammatory responses by supersulfides (2024)
  • Author(s): Tsutsuki Hiroyasu、Zhang Tianli、Akaike Takaaki、Sawa Tomohiro
  • Journal Title: International Immunology Volume: 36 Issue: 4 Pages: 143-154
  • DOI: 10.1093/intimm/dxad057
  • Peer Reviewed / Open Access
• [Journal Article] Longevity control by supersulfide-mediated mitochondrial respiration and regulation of protein quality (2024)
  • Author(s): Nishimura Akira、Yoon Sunghyeon、Matsunaga Tetsuro、Ida Tomoaki、Jung Minkyung、Ogata Seiryo、Morita Masanobu、Yoshitake Jun、Unno Yuka、Barayeu Uladzimir、Takata Tsuyoshi、Takagi Hiroshi、Motohashi Hozumi、van der Vliet Albert、Akaike Takaaki
  • Journal Title: Redox Biology Volume: 69 Pages: 103018-103018
  • DOI: 10.1016/j.redox.2023.103018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantitative profiling of supersulfides naturally occurring in dietary meats and beans (2024)
  • Author(s): Kasamatsu Shingo、Kinno Ayaka、Miura Chiharu、Hishiyama Jun-ichi、Fukui Kensuke、Kure Shoji、Tsumura Kazunobu、Ida Tomoaki、Matsunaga Tetsuro、Akaike Takaaki、Ihara Hideshi
  • Journal Title: Analytical Biochemistry Volume: 685 Pages: 115392-115392
  • DOI: 10.1016/j.ab.2023.115392
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Supersulfides support bone growth by promoting chondrocyte proliferation in the growth plates (2024)
  • Author(s): Sasama Yuji、Yoshimura Kentaro、Hoshino Marie、Sasa Kiyohito、Akaike Takaaki、Morita Masanobu、Baba Kazuyoshi、Shirota Tatsuo、Miyamoto Yoichi
  • Journal Title: Journal of Oral Biosciences Volume: 66 Issue: 1 Pages: 76-81
  • DOI: 10.1016/j.job.2023.11.004
  • Peer Reviewed / Open Access
• [Journal Article] Redox regulation of xenobiotics by reactive sulfur and supersulfide species (2024)
  • Author(s): Zhang Tianli、Akaike Takaaki、Sawa Tomohiro
  • Journal Title: Antioxidants Redox Signaling Volume: 40 Issue: 10-12 Pages: 679-690
  • DOI: 10.1089/ars.2022.0172
  • Peer Reviewed / Open Access
• [Journal Article] Alkyl gallates inhibit serine O-acetyltransferase in bacteria and enhance susceptibility of drug-resistant Gram-negative bacteria to antibiotics (2023)
  • Author(s): Toyomoto Touya、Ono Katsuhiko、Shiba Tomoo、Momitani Kenta、Zhang Tianli、Tsutsuki Hiroyasu、Ishikawa Takeshi、Hoso Kanae、Hamada Koma、Rahman Azizur、Wen Liping、Maeda Yosuke、Yamamoto Keiichi、Matsuoka Masao、Hanaoka Kenjiro、Niidome Takuro、Akaike Takaaki、Sawa Tomohiro
  • Journal Title: Frontiers in Microbiology Volume: 14 Pages: 1276447-1276447
  • DOI: 10.3389/fmicb.2023.1276447
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Supersulfide biology and translational medicine for disease control (2023)
  • Author(s): Barayeu Uladzimir、Sawa Tomohiro、Nishida Motohiro、Wei Fan-Yan、Motohashi Hozumi、Akaike Takaaki
  • Journal Title: British Journal of Pharmacology Volume: - Pages: 1-11
  • DOI: 10.1111/bph.16271
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts (2023)
  • Author(s): Kasamatsu Shingo、Owaki Takuma、Komae Somei、Kinno Ayaka、Ida Tomoaki、Akaike Takaaki、Ihara Hideshi
  • Journal Title: Redox Biology Volume: 67 Pages: 102875-102875
  • DOI: 10.1016/j.redox.2023.102875
  • Peer Reviewed / Open Access
• [Journal Article] Hypoxic erythrocytes mediate cardioprotection through activation of soluble guanylate cyclase and release of cyclic GMP (2023)
  • Author(s): Yang Jiangning、Sundqvist Michaela L.、Zheng Xiaowei、Jiao Tong、Collado Aida、Tratsiakovich Yahor、Mahdi Ali、Tengbom John、Mergia Evanthia、Catrina Sergiu-Bogdan、Zhou Zhichao、Carlstrom Mattias、Akaike Takaaki、Cortese-Krott Miriam M.、Weitzberg Eddie、Lundberg Jon O.、Pernow John
  • Journal Title: Journal of Clinical Investigation Volume: 133 Issue: 17
  • DOI: 10.1172/jci167693
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Persulfide biosynthesis conserved evolutionarily in all organisms (2023)
  • Author(s): Ogata Seiryo、Matsunaga Tetsuro、Jung Minkyung、Barayeu Uladzimir、Morita Masanobu、Akaike Takaaki
  • Journal Title: Antioxidants Redox Signaling Volume: 39 Issue: 13-15 Pages: 983-999
  • DOI: 10.1089/ars.2023.0405
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Supersulfide catalysis for nitric oxide and aldehyde metabolism (2023)
  • Author(s): Kasamatsu Shingo、Nishimura Akira、Alam Md. Morshedul、Morita Masanobu、Shimoda Kakeru、Matsunaga Tetsuro、Jung Minkyung、Ogata Seiryo、Barayeu Uladzimir、Ida Tomoaki、Nishida Motohiro、Nishimura Akiyuki、Motohashi Hozumi、Akaike Takaaki
  • Journal Title: Science Advances Volume: 9 Issue: 33 Pages: 1-11
  • DOI: 10.1126/sciadv.adg8631
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sulfur metabolic response in macrophage limits excessive inflammatory response by creating a negative feedback loop. (2023)
  • Author(s): Takeda H, Murakami S, Liu Z, Sawa T, Takahashi M, Izumi Y, Bamba T, Sato H, Akaike T, Sekine H, Motohashi H*.
  • Journal Title: Redox Biology Volume: 65 Pages: 102834-102834
  • DOI: 10.1016/j.redox.2023.102834
  • Peer Reviewed / Open Access
• [Journal Article] Supersulphides provide airway protection in viral and chronic lung diseases (2023)
  • Author(s): Matsunaga Tetsuro、Sano Hirohito、Takita Katsuya、Morita Masanobu、Yamanaka Shun、Ichikawa Tomohiro、Numakura Tadahisa、Ida Tomoaki、Jung Minkyung、Ogata Seiryo、Yoon Sunghyeon、Fujino Naoya、Kyogoku Yorihiko、Akaike Takaaki、et al.
  • Journal Title: Nature Communications Volume: 14 Issue: 1 Pages: 4476-4476
  • DOI: 10.1038/s41467-023-40182-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Glutathione trisulfide prevents lipopolysaccharide-induced retinal inflammation via inhibition of proinflammatory cytokine production in glial cells (2023)
  • Author(s): Tawarayama Hiroshi、Umeki Kota、Inoue-Yanagimachi Maki、Takahashi Naoki、Hasegawa Hirokazu、Himori Noriko、Tsuda Satoru、Kunikata Hiroshi、Akaike Takaaki、Nakazawa Toru
  • Journal Title: Scientific Reports Volume: 13 Issue: 1 Pages: 11513-11513
  • DOI: 10.1038/s41598-023-38696-4
  • Peer Reviewed / Open Access
• [Journal Article] Development of methods for quantitative determination of the total and reactive polysulfides: Reactive polysulfide profiling in vegetables (2023)
  • Author(s): Kasamatsu Shingo、Kinno Ayaka、Hishiyama Jun-ichi、Akaike Takaaki、Hideshi IharaIhara Hideshi
  • Journal Title: Food Chemistry Volume: 413 Pages: 135610-135610
  • DOI: 10.1016/j.foodchem.2023.135610
  • Peer Reviewed / Open Access
• [Journal Article] Presence of <i>Helicobacter cinaedi</i> in Atherosclerotic Abdominal Aortic Aneurysmal Wall (2023)
  • Author(s): Horii Shinichiro、Sugawara Hirofumi、Goto Hitoshi、Hashimoto Munetaka、Matsunaga Tetsuro、Akamatsu Daijirou、Tajima Yuta、Umetsu Michihisa、Akaike Takaaki、Kamei Takashi
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 261 Issue: 1 Pages: 35-41
  • DOI: 10.1620/tjem.2023.J049
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cystathionine γ-lyase self-inactivates by polysulfidation during cystine metabolism (2023)
  • Author(s): Araki Shoma、Takata Tsuyoshi、Ono Katsuhiko、Sawa Tomohiro、Kasamatsu Shingo、Ihara Hideshi、Kumagai Yoshito、Akaike Takaaki、Watanabe Yasuo、Tsuchiya Yukihiro
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 12 Pages: 9982-9982
  • DOI: 10.3390/ijms24129982
  • Peer Reviewed / Open Access
• [Journal Article] Reactive sulfur species omics analysis in the brain tissue of the 5xFAD mouse model of Alzheimer’s disease (2023)
  • Author(s): Kinno Ayaka、Kasamatsu Shingo、Akaike Takaaki、Ihara Hideshi
  • Journal Title: Antioxidants Volume: 12 Issue: 5 Pages: 1105-1105
  • DOI: 10.3390/antiox12051105
  • Peer Reviewed / Open Access
• [Journal Article] Synthesis of sulfides and persulfides is not impeded by disruption of three canonical enzymes in sulfur metabolism (2023)
  • Author(s): Zainol Abidin Qamarul Hafiz、Ida Tomoaki、Morita Masanobu、Matsunaga Tetsuro、Nishimura Akira、Jung Minkyung、Hassan Naim、Takata Tsuyoshi、Ishii Isao、Kruger Warren、Wang Rui、Motohashi Hozumi、Tsutsui Masato、Akaike Takaaki
  • Journal Title: Antioxidants Volume: 12 Issue: 4 Pages: 868-868
  • DOI: 10.3390/antiox12040868
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Polysulfide metabolizing enzymes influence SqrR-mediated sulfide-induced transcription by impacting intracellular polysulfide dynamics (2023)
  • Author(s): Shimizu Takayuki、Ida Tomoaki、Antelo Giuliano T、Ihara Yuta、Fakhoury Joseph N、Masuda Shinji、Giedroc David P、Akaike Takaaki、Capdevila Daiana A、Masuda Tatsuru
  • Journal Title: PNAS Nexus Volume: 2 Issue: 3 Pages: 1-11
  • DOI: 10.1093/pnasnexus/pgad048
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Contribution of NRF2 to sulfur metabolism and mitochondrial activity (2023)
  • Author(s): Alam Md Morshedul、Kishino Akihiro、Sung Eunkyu、Sekine Hiroki、Abe Takaaki、Murakami Shohei、Akaike Takaaki、Motohashi Hozumi
  • Journal Title: Redox Biology Volume: 60 Pages: 102624-102624
  • DOI: 10.1016/j.redox.2023.102624
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Echinochrome prevents sulfide catabolism-associated chronic heart failure after myocardial infarction in mice (2023)
  • Author(s): Tang Xiaokang、Nishimura Akiyuki、Ariyoshi Kohei、Nishiyama Kazuhiro、Kato Yuri、Vasileva Elena A.、Mishchenko Natalia P.、Fedoreyev Sergey A.、Stonik Valentin A.、Kim Hyoung-Kyu、Han Jin、Kanda Yasunari、Umezawa Keitaro、Urano Yasuteru、Akaike Takaaki、Nishida Motohiro
  • Journal Title: Marine Drugs Volume: 21 Issue: 1 Pages: 52-52
  • DOI: 10.3390/md21010052
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cysteine hydropersulfide reduces lipid peroxidation and protects against myocardial ischaemia-reperfusion injury - Are endogenous persulfides mediators of ischaemic preconditioning? (2023)
  • Author(s): Griffiths Kayleigh、Ida Tomoaki、Morita Masanobu、Lamb Reece J.、Lee Jordan J.、Frenneaux Michael P.、Fukuto Jon M.、Akaike Takaaki、Feelisch Martin、Madhani Melanie
  • Journal Title: Redox Biology Volume: 60 Pages: 102605-102605
  • DOI: 10.1016/j.redox.2023.102605
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 8-Nitro-cGMP suppresses mineralization by mouse osteoblasts (2022)
  • Author(s): Kaneko K, Miyamoto Y, Ida T, Morita M, Yoshimura K, Nagasaki K, Toba K, Sugisaki R, Motohashi H, Akaike T, Chikazu D, Kamijo R
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 71 Issue: 3 Pages: 191-197
  • DOI: 10.3164/jcbn.21-129
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access
• [Journal Article] Cystine-dependent antiporters buffer against excess intracellular reactive sulfur species-induced stress (2022)
  • Author(s): Akiyama Masahiro、Unoki Takamitsu、Aoki Hanako、Nishimura Akiyuki、Shinkai Yasuhiro、Warabi Eiji、Nishiyama Kazuhiro、Furumoto Yuka、Anzai Naohiko、Akaike Takaaki、Nishida Motohiro、Kumagai Yoshito
  • Journal Title: Redox Biology Volume: 57 Pages: 102514-102514
  • DOI: 10.1016/j.redox.2022.102514
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides (2022)
  • Author(s): Kunikata Hiroshi、Tawarayama Hiroshi、Tsuda Satoru、Akaike Takaaki、Nakazawa Toru
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 19243-12316
  • DOI: 10.1038/s41598-022-21677-4
  • Peer Reviewed / Open Access
• [Journal Article] Coupled discrete phase model and Eulerian wall film model for numerical simulation of respiratory droplet generation during coughing (2022)
  • Author(s): Anzai Hitomi、Shindo Yugo、Kohata Yutaro、Hasegawa Masahiro、Takana Hidemasa、Matsunaga Tetsuro、Akaike Takaaki、Ohta Makoto
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 14849-14849
  • DOI: 10.1038/s41598-022-18788-3
  • Peer Reviewed / Open Access
• [Journal Article] Shining a light on SSP4: A comprehensive analysis and biological applications for the detection of sulfane sulfurs (2022)
  • Author(s): Shieh Meg、Ni Xiang、Xu Shi、Lindahl Stephen P.、Yang Moua、Matsunaga Tetsuro、Flaumenhaft Robert、Akaike Takaaki、Xian Ming
  • Journal Title: Redox Biology Volume: 56 Pages: 102433-102433
  • DOI: 10.1016/j.redox.2022.102433
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Subtilase cytotoxin from Shiga-toxigenic Escherichia coli impairs the inflammasome and exacerbates enteropathogenic bacterial infection. (2022)
  • Author(s): Tsutsuki Hiroyasu, Zhang Tianli, Yahiro Kinnosuke, Ono Katsuhiko, Fujiwara Yukio, Iyoda Sunao, Wei Fan-Yan, Monde Kazuaki, Seto Kazuko, Ohnishi Makoto, Oshiumi Hiroyuki, Akaike Takaaki, Sawa Tomohiro.
  • Journal Title: iScience Volume: 25 Issue: 4 Pages: 104050-104050
  • DOI: 10.1016/j.isci.2022.104050
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of nitric oxide/reactive oxygen species redox signaling by nNOS splicing variants. (2022)
  • Author(s): Kasamatsu Shingo, Tsutsuki Hiroyasu, Ida Tomoaki, Sawa Tomohiro, Watanabe Yasuo, Akaike Takaaki, Ihara Hideshi.
  • Journal Title: Nitric Oxide Volume: 120 Pages: 44-52
  • DOI: 10.1016/j.niox.2022.01.004
  • Peer Reviewed
• [Journal Article] What triggers inflammation in COVID-19? (2022)
  • Author(s): Sawa Tomohiro, Akaike Takaaki.
  • Journal Title: eLife Volume: 11
  • DOI: 10.7554/elife.76231
  • Peer Reviewed / Open Access
• [Journal Article] Redox-dependent internalization of the purinergic P2Y6 receptor limits colitis progression (2022)
  • Author(s): Nishiyama Kazuhiro、Nishimura Akiyuki、Shimoda Kakeru、Tanaka Tomohiro、Kato Yuri、Shibata Takahiro、Tanaka Hiroshi、Kurose Hitoshi、Azuma Yasu-Taka、Ihara Hideshi、Kumagai Yoshito、Akaike Takaaki、Eaton Philip、Uchida Koji、Nishida Motohiro
  • Journal Title: Science Signaling Volume: 15 Issue: 716 Pages: 716-716
  • DOI: 10.1126/scisignal.abj0644
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] GRIM-19 is a target of mycobacterial Zn2+ metalloprotease 1 and indispensable for NLRP3 inflammasome activation. (2022)
  • Author(s): Kurane, T., Matsunaga, T., Ida, T., Sawada, K., Nishimura, A., Fukui, M., Umemura, M., Nakayama, M., Ohara, N., Matsumoto, S., Akaike, T., Matsuzaki, G., Takaesu, G.
  • Journal Title: FASEB Journal Volume: 36 Issue: 1
  • DOI: 10.1096/fj.202101074rr
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemical biology of reactive sulfur species: Hydrolysis-driven equilibrium of polysulfides as a determinant of physiological functions. (2022)
  • Author(s): Sawa Tomohiro, Takata Tsuyoshi, Matsunaga Tetsuro, Ihara Hideshi, Motohashi Hozumi, Akaike Takaaki.
  • Journal Title: Antioxidants & Redox Signaling Volume: 36 Issue: 4-6 Pages: 327-336
  • DOI: 10.1089/ars.2021.0170
  • Peer Reviewed / Open Access
• [Journal Article] Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay. (2021)
  • Author(s): Hayashi Kyoko, Asai Satomi, Umezawa Kazuo, Kakizoe Hidehumi, Miyachi Hayato, Morita Masanobu, Akaike Takaaki, Kuno Hitoshi, Komatsu Satoko, Watanabe Takumi, Kawahara Toshio.
  • Journal Title: Journal of Clinical Laboratory Analysis Volume: 36 Issue: 1
  • DOI: 10.1002/jcla.24146
  • Peer Reviewed / Open Access
• [Journal Article] Reprogrammed transsulfuration promotes basal-like breast tumor progression via realigning cellular cysteine persulfidation. (2021)
  • Author(s): Erdelyi Katalin, Ditroi Tamas, Johansson Henrik J., Czikora Agnes, Balog Noemi, Silwal-Pandit Laxmi, Ida Tomoaki, Olasz Judit, Hajdu Dorottya, Matrai Zoltan, Csuka Orsolya, Uchida Koji, Tovari Jozsef, Engebraten Olav, Akaike Takaaki, Borresen Dale Anne-Lise, Kasler Miklos, Lehtio Janne, Nagy Peter.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 118 Issue: 45
  • DOI: 10.1073/pnas.2100050118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Methods in sulfide and persulfide research. (2021)
  • Author(s): Takata Tsuyoshi, Jung Minkyung, Matsunaga Tetsuro, Ida Tomoaki, Morita Masanobu, Motohashi Hozumi, Shen Xinggui, Kevil Christopher G., Fukuto Jon M., Akaike Takaaki.
  • Journal Title: Nitric Oxide Volume: 116 Pages: 47-64
  • DOI: 10.1016/j.niox.2021.09.002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sulfide catabolism ameliorates hypoxic brain injury. (2021)
  • Author(s): Marutani E, Morita M, Hirai S, Kai S, Grange RMH, Miyazaki Y, Nagashima F, Traeger L, Magliocca A, Ida T, Matsunaga T, Batten A, Li R, Tanaka T, Ikeda T, Nakagawa A, Atochin DN, Ihara H, Olenchock BA, Shen X, Nishida M, Hanaoka K, Kevil CG, Xian M, Bloch DB, Akaike T, Hindle AG, Motohashi H, Ichinose F, et al.
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 3108-3108
  • DOI: 10.1038/s41467-021-23363-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cysteine hydropersulfide inactivates β-lactam antibiotics to form ring opened carbothioic S-acids in bacteria. (2021)
  • Author(s): Ono, K., Kitamura, Y., Zhang, T., Tsutsuki, H., Rahman, A., Akaike, T., Ihara, T., Sawa, T.
  • Journal Title: ACS Chem Biol. Volume: 16 Issue: 4 Pages: 731-739
  • DOI: 10.1021/acschembio.1c00027
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comment on “Evidence that the ProPerDP method is inadequate for protein persulfidation detection due to lack of specificity”. (2021)
  • Author(s): Doka Eva, Arner Elias SJ, Schmidt Edward E, Dick Tobias P, Vliet Albert van der, Yang Jing, Szatmari Reka, Ditroi Tamas, Wallace John L, Cirino Giuseppe , Olson Kenneth, Motohashi Hozumi, Fukuto Jon M, Pluth Michael D, Feelisch Martin, Akaike Takaaki, Wink David A, Ignarro Louis J, Nagy Peter.
  • Journal Title: Science Advances Volume: 7 Issue: 17
  • DOI: 10.1126/sciadv.abe7006
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Supersulfide redox biology in life science and its translational medicine for disease control (2024)
  • Author(s): Takaaki Akaike
  • Organizer: International Symposium “Signals for Human, Animal and Planetary Health: From Metabolites To Biological Interactions” (Tokyo Univ.)
  • Int'l Joint Research / Invited
• [Presentation] 超硫黄分子のラマン分光イメージングを駆使した定量分析 (2023)
  • Author(s): 赤池 孝章
  • Organizer: CREST「多細胞」領域，第5回領域会議プログラム・連携課題（浜松）
• [Presentation] Supersulfides-mediated cellular protection from lipid oxidation and ferroptosis (2023)
  • Author(s): Takaaki Akaike
  • Organizer: 3rd Annual Meeting of the DFG founded Priority Programme (Heidelberg, Germany)
  • Int'l Joint Research / Invited
• [Presentation] 超硫黄分子の発見と生理機能の解明 (2023)
  • Author(s): 赤池孝章
  • Organizer: 第96回日本生化学会大会・特別講演（福岡）
  • Invited
• [Presentation] 定量的超硫黄オミックス・イメージング技術の開発と標準化プロトコールの確立 (2023)
  • Author(s): 赤池孝章
  • Organizer: 学術変革領域研究(A) 「硫黄生物学」第3回領域会議・超硫黄フロンティアシンポジウム2023（熊本）
• [Presentation] Supersulfide biology and translational medicine for disease control (2023)
  • Author(s): Takaaki Akaike
  • Organizer: Chemical Biology/Redox Biology Seminar (Rhode Island, USA)
  • Int'l Joint Research / Invited
• [Presentation] 超硫黄分子によるエネルギー産生と過酸化脂質・カルボニル代謝制御 (2023)
  • Author(s): 赤池孝章
  • Organizer: 名古屋大学大学院生命農学研究科・特別セミナー（名古屋）
  • Invited
• [Presentation] 無機超硫黄分子の検出定量と化学的反応性の理解 (2023)
  • Author(s): 赤池孝章
  • Organizer: 学術変革領域「硫黄生物学」領域プログレスミーティング（オンライン）
• [Presentation] 活性酸素シグナル~酸素生物学~そして硫黄生物学：三つの領域のレドックス生命科学研究から俯瞰される世界 (2023)
  • Author(s): 赤池孝章
  • Organizer: 第96回日本細菌学会総会（姫路）
  • Invited
• [Presentation] Nitric Oxide Synthases, a New Family of NADPH-Dependent Sulfur Oxidoreductases (2023)
  • Author(s): Takaaki Akaike
  • Organizer: Nitric Oxide Gordon Research Conference
  • Int'l Joint Research / Invited
• [Presentation] 空間生体情報メタオミックス解析と呼気医療 (2023)
  • Author(s): 赤池孝章
  • Organizer: JASIS関西2023トピックスセミナー（大阪）
  • Invited
• [Presentation] 環化超硫黄分子の生体内検出と機能解明 (2023)
  • Author(s): 赤池孝章
  • Organizer: CREST多細胞領域 第4回領域会議プログラム（名古屋）
  • Invited
• [Presentation] Physiological formation and function of new supersulfide, cyclic octa-sulfur in mammals (2023)
  • Author(s): Takaaki Akaike
  • Organizer: 3rd STINT-JSPS Redox Biology Conference (Stockholm)
  • Int'l Joint Research / Invited
• [Presentation] The physiological functions of cyclic octa-sulfur (S8) formed endogenously in diverse organisms including mammals (2022)
  • Author(s): Takaaki Akaike
  • Organizer: Redox week in Sendai 2022 (Sendai)
  • Int'l Joint Research / Invited
• [Presentation] Sulfur biology and redox signaling in energy metabolism, infection, and immunity (2022)
  • Author(s): Takaaki Akaike
  • Organizer: The 15th Korea-Japan International Symposium on Microbiology (Korea)
  • Int'l Joint Research / Invited
• [Presentation] 呼気オミックスから空間バイオミックスへの新展開 (2022)
  • Author(s): 赤池孝章
  • Organizer: レドックス R&D 戦略委員会第3回企画シンポジウム
  • Invited
• [Presentation] Cyclo-octasulfur, S8, formed endogenously in mammals: its biosynthesis and physiological functions (2022)
  • Author(s): Takaaki Akaike
  • Organizer: GRC “Thiol-Based Redox Regulation and Signaling: The Chemical Biology of Sulfur”
  • Int'l Joint Research / Invited
• [Presentation] Reactive persulfide regulation of redox signaling and immune response (2022)
  • Author(s): Takaaki Akaike
  • Organizer: 6th World Congress on Hydrogen Sulfide in Biology & Medicine Keynote Lecture, Molecular Mechanisms (online)
  • Int'l Joint Research / Invited
• [Presentation] 呼気オミックスによる硫黄代謝解析と新型コロナ感染症 (2022)
  • Author(s): 赤池孝章
  • Organizer: 第99回日本生理学会大会
  • Invited
• [Presentation] 超硫黄分子の発見と感染防御・免疫・代謝制御に関する研究 (2022)
  • Author(s): 赤池孝章
  • Organizer: 第3回 太田原豊一賞 受賞講演
  • Invited
• [Presentation] 活性酸素シグナル~酸素生物学~そして硫黄生物学:三つの領域を超えて見えてきた世界 (2022)
  • Author(s): 赤池孝章
  • Organizer: 第2回レドックスR&D戦略委員会 春のシンポジウム ―最先端技術が切り拓くレドックスバイオロジー
  • Invited
• [Presentation] 呼気オミックスと未来型医療:ヒューマンエアローム事業の創成 (2022)
  • Author(s): 赤池孝章
  • Organizer: SAカレッジ21年度コースIII月例会
  • Invited
• [Presentation] 定量的超硫黄オミックス・イメージング技術の開発と標準化プロトコールの確立 (2021)
  • Author(s): 赤池孝章
  • Organizer: 令和3(2021)年度 学術変革領域研究(A) 新興硫黄生物学が拓く生命原理変革(硫黄生物学) 第2回月例領域プログレスミーティング
  • Invited
• [Presentation] 硫黄呼吸の生命進化論 (2021)
  • Author(s): 赤池孝章
  • Organizer: 第20回日本ミトコンドリア学会
  • Invited
• [Presentation] レドックスとエネルギー代謝の超硫黄パラダイム (2021)
  • Author(s): 赤池孝章
  • Organizer: 第94回日本生化学会大会
  • Invited
• [Presentation] 最近の超硫黄計測技術の展開 (2021)
  • Author(s): 赤池孝章
  • Organizer: 令和3(2021)年度学術変革領域研究(A) 新興硫黄生物学が拓く生命原理変革(硫黄生物学) 第1回総括班会議
  • Invited
• [Presentation] 硫黄呼吸の革新的イメージングの開発と応用 (2021)
  • Author(s): 赤池孝章
  • Organizer: 新領域創成のための挑戦研究デュオ~Frontier Research in Duo (FRiD)~ 公開セミナー
  • Invited
• [Presentation] 呼気オミックスと未来型医療 (2021)
  • Author(s): 赤池孝章
  • Organizer: 第46回日本医用マススペクトル学会
  • Invited
• [Presentation] 超硫黄分子による生体防御学の新たな潮流 (2021)
  • Author(s): 赤池孝章
  • Organizer: 第32回日本生体防御学会学術集会
  • Invited
• [Presentation] 超硫黄分子による感染免疫制御とエネルギー代謝 (2021)
  • Author(s): 赤池孝章
  • Organizer: 第41回阿蘇シンポジウム
  • Invited
• [Presentation] 超硫黄分子による電子転移反応とエネルギー代謝 (2021)
  • Author(s): 赤池孝章
  • Organizer: 生理研研究会2021「生命を支える硫黄生物学研究の最前線」
  • Invited
• [Presentation] Sulfur respiration highly conserved and evolved in microbiota and organisms including humans. (2021)
  • Author(s): Takaaki Akaike
  • Organizer: World Microbe Forum 2021
  • Invited
• [Presentation] 呼気オミックスによる新型コロナ感染診断法と未来型医療 (2021)
  • Author(s): 赤池孝章
  • Organizer: 第81回分析化学討論会
  • Invited
• [Presentation] 超硫黄代謝と呼気オミックス (2021)
  • Author(s): 赤池孝章
  • Organizer: 第3回国際活性硫黄研究会
  • Int'l Joint Research / Invited
• [Presentation] 超硫黄生物学:エネルギー代謝とレドックスシグナル (2021)
  • Author(s): 赤池孝章
  • Organizer: 第74回日本酸化ストレス学会・第21回日本NO学会合同学術集会
  • Invited
• [Presentation] 新型コロナの呼気オミックス解析と未来型呼気医療 (2021)
  • Author(s): 赤池孝章
  • Organizer: 第30回バイオメディカル研究会「健康を測るセンシング技術」
  • Invited
• [Book] 呼吸器疾患最新の治療2025-2026（超硫黄分子による呼吸器疾患制御と呼気オミックス） (2024)
  • Author(s): 緒方星陵, 松永哲郎, 赤池孝章
  • Total Pages: 6
  • Publisher: 南江堂
• [Book] 酸化ストレスの医学 改訂第３版（超硫黄分子の化学と生理機能） (2024)
  • Author(s): 緒方星陵, Uladzimir Barayeu, 赤池孝章
  • Total Pages: 7
  • Publisher: 診断と治療社
• [Book] メディカルサイエンスダイジェスト（［特集］環境化学物質と生体応答） (2023)
  • Author(s): 赤池孝章
  • Total Pages: 56
  • Publisher: ニューサイエンス社
• [Book] メディカルサイエンスダイジェスト（環境物質と生命の起源：超硫黄生物学プロローグ） (2023)
  • Author(s): 松永哲郎, 赤池孝章
  • Total Pages: 5
  • Publisher: ニューサイエンス社
• [Book] Sulfurtransferases（A persulfide shield） (2023)
  • Author(s): Hamid Hisyam Abdul、Takata Tsuyoshi、Matsunaga Tetsuro、Akaike Takaaki
  • Total Pages: 16
  • Publisher: Elsevier
• [Book] 呼気オミックスと個別化医療 (2021)
  • Author(s): 赤池孝章、松永哲郎、井田智章、高田 剛、Jung Minkyung、守田匡伸、本橋ほづみ
  • Total Pages: 94
  • Publisher: Precision Medicine (株)北隆館
• [Book] 生命を支える超硫黄分子の代謝と革新的な計測技術 (2021)
  • Author(s): 本橋ほづみ、赤池孝章
  • Total Pages: 204
  • Publisher: 生化学 (公社)日本生化学会
• [Book] 超硫黄分子の化学と代謝:超硫黄生物学の創成とオミックス先制医療への展望 (2021)
  • Author(s): 赤池孝章、松永哲郎、高田 剛
  • Total Pages: 204
  • Publisher: 生化学 (公社)日本生化学会
• [Book] 超硫黄代謝物の化学と代謝 (2021)
  • Author(s): 高田 剛、松永哲郎、赤池孝章
  • Total Pages: 139
  • Publisher: 実験医学 (株)羊土社
• [Remarks] 東北大学大学院医学系研究科環境医学分野ホームページ
  • URL: http://www.toxicosci.med.tohoku.ac.jp/index.html
• [Remarks] Research Mapマイポータル
  • URL: https://researchmap.jp/AkaikeT
• [Remarks] 「島津製作所×東北大学 超硫黄生命科学共創研究所」を設置
  • URL: https://www.tohoku.ac.jp/japanese/2024/03/press20240313-04-shimazu.html
• [Remarks] 「超硫黄分子」の寿命延長効果を発見 ～新たなサプリメントや健康法の開発に期待～
  • URL: https://www.tohoku.ac.jp/japanese/2024/01/press20240119-01-supersulfides.html
• [Remarks] 学術変革領域研究(A) 新興硫黄生物学が拓く生命原理変革(硫黄生物学)ホームページ
  • URL: https://supersulfide-proj.com/
• [Patent(Industrial Property Rights)] 活性硫黄の定量方法 (2022)
  • Inventor(s): 國澤研大，遠山敦 彦，赤池孝章，飯伏 翼
  • Industrial Property Rights Holder: 國澤研大，遠山敦 彦，赤池孝章，飯伏 翼
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2022-209942
  • Filing Date: 2022
• [Patent(Industrial Property Rights)] 感染症のバイオマーカ (2022)
  • Inventor(s): 赤池孝章
  • Industrial Property Rights Holder: 赤池孝章
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2021-084723
  • Filing Date: 2022
  • Overseas
• [Funded Workshop] The 1st International G-ReXS Conference in Sendai (2024)
• [Funded Workshop] Redox Week in Sendai 2022 (2022)
• [Funded Workshop] 第3回国際活性硫黄研究会 (2021)