| Budget Amount *help |
¥83,590,000 (Direct Cost: ¥64,300,000、Indirect Cost: ¥19,290,000)
Fiscal Year 2014: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2013: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2012: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2011: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Outline of Final Research Achievements |
“Total biosynthesis” is a novel approach to provide natural products. To achieve total biosynthesis of fungal metabolites, Aspergillus oryzae expression system is one of a promising tool due to highly reliable expression of biosynthetic enzyme genes. Complete stepwise reconstitution of biosynthetic machinery is a key issue of total biosynthesis. This provides not only information of complete reaction sequence but target natural product together with all reaction intermediates which allow us to study enzymatic mechanism of a specific step. We have achieved genome mining, total biosynthesis of structurally unique metabolites and elucidation of complex reaction mechanism of modification enzymes.
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