| Budget Amount *help |
¥138,060,000 (Direct Cost: ¥106,200,000、Indirect Cost: ¥31,860,000)
Fiscal Year 2014: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2013: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2012: ¥35,880,000 (Direct Cost: ¥27,600,000、Indirect Cost: ¥8,280,000)
Fiscal Year 2011: ¥33,930,000 (Direct Cost: ¥26,100,000、Indirect Cost: ¥7,830,000)
Fiscal Year 2010: ¥36,920,000 (Direct Cost: ¥28,400,000、Indirect Cost: ¥8,520,000)
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| Outline of Final Research Achievements |
We examined lymphocyte intranodal migration and antigen recognition to clarify the the functions and regulations of LFA-1 mainly using two-photon imaging techniques. We demonstrate: (1) there are two migration modes in peripheral lymph nodes; chemokine-independent random migration and chemokine-dependent directional migration, the latter of which depends on LFA-1 binding to dendritic ICAM-1, (2) thymocytes exhibit fast migration within the medulla of the thymus, and recognize self-antigen, which require LFA-1/ICAM-1 dependent adhesion regulated by Mst1, (3) regulatory T cells show migratory immune synapses in vitro and in lymph nodes , which regulated by Mst1. We further establish single-molecule analysis of LFA-1/ICAM-1 bindings in immune synapse in which Rap1/Mst-1 regulate high-affinity bindings.
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