• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of new intravital fluorescence imaging system and analysis of the cancer microenvironment

Planned Research

Project AreaMutli-dimensional fluorescence live imaging of cellular function and molecular activity
Project/Area Number 22113004
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionEhime University

Principal Investigator

IMAMURA Takeshi  愛媛大学, 医学(系)研究科(研究院), 教授 (70264421)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥82,550,000 (Direct Cost: ¥63,500,000、Indirect Cost: ¥19,050,000)
Fiscal Year 2014: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2013: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2012: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2011: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2010: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Keywordsバイオテクノロジー / シグナル伝達 / 遺伝子 / 生体分子 / 細胞・組織
Outline of Final Research Achievements

We have developed a new biological fluorescence observation system in combination with a fluorescence zoom microscopy and a confocal laser microscopy. Using the mew microscopy system, cancer cells and blood vessels could be visualized in real-time from whole tumor tissues of mouse to single cell levels. I addition, we developed several cell lines that can be visualized cell cycle progression and TGF-beta signaling. Finally, cell cycle and TGF-beta signaling of cancer cells as well as protease activity of tumor microenvironment including Cathepsins and MMPs were visualized and evaluated in vivo.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Invited: 3 results)

  • [Journal Article] In vivo subcellular imaging of tumors in mouse models using a fluorophore-conjugated anti-CEA antibody in TPEM2014

    • Author(s)
      Shigehiro Koga, Yusuke Oshima, Naoki Honkura, Tadahiro Iimura, Kenji Kameda, Koichi Sato, Motohira Yoshida, Yuji Yamamoto, Yuji Watanabe, Atsuhiko Hikita, and Takeshi Imamura
    • Journal Title

      Cancer Science

      Volume: 105(10) Issue: 10 Pages: 1299-1306

    • DOI

      10.1111/cas.12500

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation of TGF-β family signalling by ubiquitination and deubiquitination.2013

    • Author(s)
      Imamura T, Oshima Y, Hikita A
    • Journal Title

      J Biochem.

      Volume: 154(6) Issue: 6 Pages: 481-489

    • DOI

      10.1093/jb/mvt097

    • NAID

      40019909439

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of p53 Activity through the Cooperative Action of Ski and Histone Deacetylase SIRT12011

    • Author(s)
      Inoue Y, et al
    • Journal Title

      J Bid Chem

      Volume: 286 Issue: 8 Pages: 6311-20

    • DOI

      10.1074/jbc.m110.177683

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell type-specific target selection by combinatorial binding of Smad2/3 proteins and hepatocyte nuclear factor 4α in HepG2 cells2011

    • Author(s)
      Kanki Y, Kohro T, Jiang S, Tsutsumi S, Mimura I, Suehiro JI,Wada Y, Ohta Y, Ihara S, Iwanari H, Naito M, Hamakubo T,Aburatani H, Kodama T, Minami T
    • Journal Title

      EMBO J

      Volume: 286 Issue: 34 Pages: 29848

    • DOI

      10.1074/jbc.m110.217745

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RB1CC1 protein positively regulates transforming growth factor-beta signaling through the modulation of Arkadia E3 ubiquitin ligase activity2011

    • Author(s)
      Koinuma D
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286 Issue: 37 Pages: 32502-32512

    • DOI

      10.1074/jbc.m111.227561

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of p53 Activity through the Cooperative Action of Ski and Histone Deacetylase SIRT12011

    • Author(s)
      Inoue Y, et al
    • Journal Title

      J Biol Chem

      Volume: 286 Pages: 6311-20

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arkadia complexes with clathrin adaptor AP2 and regulates EGF signaling2011

    • Author(s)
      Mizutani A, et al
    • Journal Title

      J Biochem

      Volume: 148 Pages: 733-41

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 蛍光生体4Dイメージングが拓く新たな形態学2014

    • Author(s)
      今村健志
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 最新の光イメージング技術のがん研究への応用2012

    • Author(s)
      今村健志
    • Organizer
      第37回組織細胞化学講習会
    • Place of Presentation
      高槻現代劇場中ホール
    • Year and Date
      2012-08-02
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] session3 Nano- and bio-imaging In vivo optical imaging in cancer research2011

    • Author(s)
      今村健志
    • Organizer
      第12回千歳光科学国際フォーラム
    • Place of Presentation
      千歳科学技術大学千歳市(招待講演)
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] In vivo optical imaging of bone metastasis2011

    • Author(s)
      今村健志
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場名古屋市(招待講演)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] がん細胞とがん微小環境のintravital imaging2011

    • Author(s)
      今村健志
    • Organizer
      がん研究分野の特性等を踏まえた支援活動援活公開シンポジウムがんの蛍光生体イメージング
    • Place of Presentation
      千里ライフサイエンスセンター豊中市(招待講演)
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光イメージングを利用した骨髄環境におけるがん細胞の細胞周期の解析2010

    • Author(s)
      今村健志
    • Organizer
      第69回日本癌学会学術総会シンポジウム8
    • Place of Presentation
      大阪国際会議場(大阪府)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光技術と蛍光技術を駆使した生体イメージング2010

    • Author(s)
      今村健志
    • Organizer
      第56回日本病理学会秋期特別総会シンポジウム1
    • Place of Presentation
      西日本総合展示場(福岡県)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] インビボ光操作と可視化の最前線~光技術を駆使した分子・細胞の機能操作とイメージング~2010

    • Author(s)
      今村健志
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド(兵庫県)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体蛍光イメージングの医学研究・医療応用

    • Author(s)
      今村健志
    • Organizer
      第65回日本皮膚科学会西部支部学術大会
    • Place of Presentation
      かごしま県民交流センター、鹿児島市
    • Related Report
      2013 Annual Research Report
    • Invited

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi