| Budget Amount *help |
¥109,850,000 (Direct Cost: ¥84,500,000、Indirect Cost: ¥25,350,000)
Fiscal Year 2014: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2013: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2012: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2010: ¥40,170,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥9,270,000)
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| Outline of Final Research Achievements |
During the last decade, multi-photon fluorescent microscopy has launched a new era. By using this advanced imaging technique in this study we have established a new system for visualizing in situ behavior of a diversity of living cells within intact tissues and organs. Among them, we succeeded in visualizing the various dynamic phenomena within bones, a mysterious organ where various kinds of hematopoietic and immune cells are produced and functioning although poorly analyzed by conventional methodology such as histological analyses with decalcified bones. Especially we have focused on the behavior of osteoclast, a kind of specialized macrophage contributing to physiological bone remodeling as well as to bone destruction in cancer metastasis. In this study we have revealed novel mechanisms controlling migration and function of different bone cells in situ and also propose the further applications of this novel methodology in bone and cancer biology.
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