Understanding the operating principles of the meso-circuitry that generates spatio-temporal patterns

• Project Area: Mesoscopic neurocircuitry: towards understanding of the functional and structural basis of brain information processing
• Project/Area Number: 22115002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: NOSE AKINAO 東京大学, 新領域創成科学研究科, 教授 (30260037)
• Co-Investigator(Kenkyū-buntansha): TAKAKO MORIMOTO 東京薬科大学, 生命科学部, 准教授 (10311648)
• Co-Investigator(Renkei-kenkyūsha): HIROSHI KOHSAKA 東京大学, 大学院新領域創成科学研究科, 助教 (20431900)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥154,960,000 (Direct Cost: ¥119,200,000、Indirect Cost: ¥35,760,000); Fiscal Year 2014: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000); Fiscal Year 2013: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000); Fiscal Year 2012: ¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000); Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000); Fiscal Year 2010: ¥52,000,000 (Direct Cost: ¥40,000,000、Indirect Cost: ¥12,000,000)
• Keywords: メゾ神経回路 / オプトジェネティクス / ショウジョウバエ / 運動回路 / カルシウムイメージング / 電気シナプス / 統計解析 / コネクトミクス / 神経回路 / イメージング / 光生理学 / 運動速度調節 / 分子遺伝学 / optogenetic / ハロロドプシン(NpHR) / optogenetics

Outline of Final Research Achievements

How spatio-temporal patterns of neural activity are generated by the central circuits is a fundamental question in neuroscience. In this study, we used larval Drosophila as a model and obtained the following results. 1) We showed that local activity of motor neurons regulates, via gap junctions, the frequency of larval locomotion. 2) We identified interneurons that regulate the speed of locomotion and turning behavior. 3) We developed statistical methods that allow automated acquisition of the activity of a neural population from 4D calcium imaging data.

Research Products

• [Journal Article] A circuit mechanism for the propagation of waves of muscle contraction in Drosophila (2016)
  • Author(s): Fushiki A, Zwart MF, Kohsaka H, Fetter RD, Cardona A, Nose A.
  • Journal Title: Elife Volume: 5 pii Pages: 13253-13253
  • DOI: 10.7554/elife.13253
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identication of Inhibitory Pre- motor Interneurons Activated at a Late Phase in a Motor Cycle during Drosophila Larval Locomotion. (2015)
  • Author(s): Itakura Y, Kohsaka H, Ohyama T, Zlatic M, Pulver SR, Nose A.
  • Journal Title: PLoS One Volume: 10 Issue: 9 Pages: e0136660-e0136660
  • DOI: 10.1371/journal.pone.0136660
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Serotonin and downstream leucokinin neurons modulate larval turning behavior in Drosophila. (2014)
  • Author(s): Okusawa S, Kohsaka H, Nose A.
  • Journal Title: The Journal of Neuroscience Volume: 34 Issue: 7 Pages: 2544-2558
  • DOI: 10.1523/jneurosci.3500-13.2014
  • Peer Reviewed / Open Access
• [Journal Article] ショウジョウバエ幼虫を用いて定型運動の制御機構を探る (2014)
  • Author(s): 能瀬聡直
  • Journal Title: 細胞工学 Volume: 33 Pages: 249-254
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion. (2013)
  • Author(s): Matsunaga T, Fushiki A, Nose A, Kohsaka H.
  • Journal Title: Journal of Visualized Experiments Volume: 77 Issue: 77
  • DOI: 10.3791/50513
  • Peer Reviewed / Open Access
• [Journal Article] Role of sensory experience in functional development of Drosophila motor circuits. (2013)
  • Author(s): Fushiki A, Kohsaka H, Nose A.
  • Journal Title: PLoS One Volume: 8 Issue: 4 Pages: 1-10
  • DOI: 10.1371/journal.pone.0062199
  • Peer Reviewed / Open Access
• [Journal Article] Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction (2012)
  • Author(s): Ai Fukui, et al.
  • Journal Title: Biology Open Volume: (In press) Issue: 4 Pages: 362-375
  • DOI: 10.1242/bio.2012448
  • Peer Reviewed
• [Journal Article] Development of larval motor circuits inDrosophila (2012)
  • Author(s): Kohsaka, H., Okusawa, S., Itakura, Y., Fushiki, A. and Nose, A.
  • Journal Title: Development, Growth andDifferentiation Volume: 54 Issue: 3 Pages: 408-419
  • DOI: 10.1111/j.1440-169x.2012.01347.x
  • Peer Reviewed
• [Journal Article] Generation of neuromuscular specificity in Drosophila : novel mechanisms revealed by new technologies. (2012)
  • Author(s): Nose A.
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 5 Pages: 62-62
  • DOI: 10.3389/fnmol.2012.00062
  • Peer Reviewed
• [Journal Article] Optical dissection of neural circuits responsible for Drosophila larval locomotion with halorhodopsin (2011)
  • Author(s): Inada K, Kohsaka H, Takasu E, Matsunaga T, Nose A
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Subunit-specific and homeostatic regulation of glutamate receptor localization by CaMKII in Drosophila neuromuscular junctions (2010)
  • Author(s): Morimoto T, Nobechi M, Komatsu A, Miyakawa H, Nose A
  • Journal Title: Neuroscience Volume: 165 Pages: 1284-1292
  • Peer Reviewed
• [Journal Article] Drosophila Tey represses transcription of a repulsive cue Toll and generates neuromuscular target specificity (2010)
  • Author(s): Inaki, M, Shinza-Kameda, M, Ismat, A., Frasch, M, Nose, A
  • Journal Title: Development Volume: 137 Pages: 2139-2146
  • Peer Reviewed
• [Presentation] Local Motoneuronal activity regulates the frequency of fictive larval locomotion in a segment-specific manner (2015)
  • Author(s): Teruyuki Matsunaga, Hiroshi Kohsaka, Akinao Nose
  • Organizer: Cold Spring Harbor laboratory, Meetings-Neurobiology of Drosophila,
  • Place of Presentation: Cold Spring Harbor laboratory, USA
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] Functional dissection of the central circuits that regulate larval locomotion. (2014)
  • Author(s): Nose, A.
  • Organizer: Behavioral Neurogenetics of larval Drosophila: Molecules, Circuits, Computation and Robotics.
  • Place of Presentation: KKRホテル熱海（熱海市）
  • Year and Date: 2014-03-09 – 2014-03-12
  • Invited
• [Presentation] Search for cholinergic interneurons that regulate larval locomotion. (2013)
  • Author(s): Hasegawa, E. and Nose, A.
  • Organizer: Neurobiology of Drosophila, CSHL Meeting
  • Place of Presentation: ニューヨーク（アメリカ）
  • Year and Date: 2013-10-01 – 2013-10-05
• [Presentation] Identication and functional analyses of interneurons in the neural network that regulates the peristaltic locomotion of Drosophila larvae. (2013)
  • Author(s): Itakura, Y., Kohsaka, H., Pulver S. and Nose, A.
  • Organizer: Neurobiology of Drosophila, CSHL Meeting
  • Place of Presentation: ニューヨーク（アメリカ）
  • Year and Date: 2013-10-01 – 2013-10-05
• [Presentation] Segmental control of motor output by pre-motor inhibitory interneurons for generating innate speed of larval locomotion in Drosophila. (2013)
  • Author(s): Kohsaka, H. and Nose, A.
  • Organizer: 第36 回日本神経科学大会
  • Place of Presentation: 稲盛財団記念館（京都市）
  • Year and Date: 2013-06-20 – 2013-06-23
• [Presentation] Simultaneous application of optogenetics and Ca2+ imaging for system-level analyses of motor circuits. (2013)
  • Author(s): Matsunaga T., Kohsaka H., Nose A.
  • Organizer: 第36 回日本神経科学大会
  • Place of Presentation: 稲盛財団記念館（京都市）
  • Year and Date: 2013-06-20 – 2013-06-23
• [Presentation] Optogenetic and electrophysiological dissection of Drosophila neural networks that regulate larval locomotion. (2013)
  • Author(s): Takagi, S., Kohsaka, H., Kazama, H., Nose, A.
  • Organizer: 第36 回日本神経科学大会
  • Place of Presentation: 稲盛財団記念館（京都市）
  • Year and Date: 2013-06-20 – 2013-06-23
• [Presentation] Optogenetic dissection of the neural circuits that regulate rhythmic movement in Drosophila larvae (2013)
  • Author(s): 能瀬聡直
  • Organizer: Symposium on Sensory Systems & Neural Circuits
  • Place of Presentation: 東京大学伊藤ホール(招待講演)
  • Year and Date: 2013-02-12
• [Presentation] Optogenetic dissection of motor circuits that regulate larval peristalsis in Drosophila (2012)
  • Author(s): 能瀬聡直
  • Organizer: Behavioral Neurogenetics of Drosophila Larva meeting at Janelia Farm
  • Place of Presentation: HHMI janelia farm research campus(米国、ヴァージニア アシュバーン)(招待講演)
  • Year and Date: 2012-10-01
• [Presentation] Optogenetic dissection of motor circuits in Drosophila larvae (2012)
  • Author(s): 能瀬聡直、高坂洋史、高木俊輔
  • Organizer: 第35回日本神経科学大会」シンポジウム
  • Place of Presentation: 名古屋国際会議場(招待講演)
  • Year and Date: 2012-09-18
• [Presentation] Imaging and Manipulating Motor Circuit Activity (2012)
  • Author(s): 能瀬聡直
  • Organizer: Developmental Neurobiology Course 2012
  • Place of Presentation: 沖縄科学技術大学院大学(招待講演)
  • Year and Date: 2012-07-27
• [Presentation] 光生理学を用いたショウジョウバエ運動回路の機能解剖 (2012)
  • Author(s): 能瀬聡直
  • Organizer: 新学術領域「大脳新皮質構築」「メゾ神経回路」合同ワークショップ
  • Place of Presentation: 仙台国際センター(招待講演)
  • Year and Date: 2012-07-25
• [Presentation] Optogenetic dissection of motor circuits that regulate larval locomotion in Drosophila、 (2012)
  • Author(s): 能瀬聡直
  • Organizer: 新学術領域「メゾ神経回路」第1回公開国際シンポジウム
  • Place of Presentation: 東京大学小柴ホール(招待講演)
  • Year and Date: 2012-07-07
• [Presentation] Temporal perturbation of neural activity in Drosophila larvae undergoing locomotion (2010)
  • Author(s): Inada, K., Kohsaka, H., Takasu, E., Nose, A.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-04
• [Presentation] Optogenetic dissection of motor circuits that regulate larval locomotion in Drosophila
  • Author(s): 能瀬聡直
  • Organizer: 新学術領域「メゾ神経回路」第1回公開国際シンポジウム
  • Place of Presentation: 東京大学小柴ホール
  • Invited
• [Presentation] 光生理学を用いたショウジョウバエ運動回路の機能解剖
  • Author(s): 能瀬聡直
  • Organizer: 新学術領域「大脳新皮質構築」「メゾ神経回路」合同ワークショップ
  • Place of Presentation: 仙台国際センター
  • Invited
• [Presentation] Imaging and Manipulating Motor Circuit Activity
  • Author(s): 能瀬聡直
  • Organizer: Developmental Neurobiology Course 2012
  • Place of Presentation: 沖縄科学技術大学院大学
  • Invited
• [Presentation] Optogenetic dissection of motor circuits in Drosophila larvae
  • Author(s): 能瀬聡直、高坂洋史、高木俊輔
  • Organizer: 第35回日本神経科学大会」シンポジウム
  • Place of Presentation: 名古屋国際会議場
  • Invited
• [Presentation] Optogenetic dissection of motor circuits that regulate larval peristalsis in Drosophila
  • Author(s): 能瀬聡直
  • Organizer: "Behavioral Neurogenetics of Drosophila Larva" meeting at Janelia Farm
  • Place of Presentation: HHMI janelia farm research campus(米国、ヴァージニア アシュバーン)
  • Invited
• [Presentation] Optogenetic dissection of the neural circuits that regulate rhythmic movement in Drosophila larvae
  • Author(s): 能瀬聡直
  • Organizer: Symposium on Sensory Systems & Neural Circuits
  • Place of Presentation: 東京大学伊藤ホール
  • Invited
• [Book] オプトジェネティクス（分担執筆、タイトル「ショウジョウバエを用いたオプトジェネティクス研究」） (2013)
  • Author(s): 高坂洋史、能瀬聡直
  • Total Pages: 13
  • Publisher: エヌ・ティー・エス
• [Remarks] 能瀬研究室
  • URL: http://bio.phys.s.u-tokyo.ac.jp
• [Remarks] メゾスコピック神経回路から探る脳の情報処理基盤
  • URL: http://www.meso-neurocircuitry.jp/
• [Remarks] 「メゾスコピック神経回路から探る脳の情報処理基盤」
  • URL: http://www.meso-neurocircuitry.jp/
• [Remarks]
  • URL: http://bio.phys.s.u-tokyo.ac.jp/
• [Remarks]
  • URL: http://bio.phys.s.u-tokyo.ac.jp/