| Budget Amount *help |
¥46,280,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥10,680,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2012: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2011: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
We conducted two-photon calcium imaging of mouse layer 2/3 motor cortex during a self-initiated lever-pull task. In the imaging session after 8-9-day training, head-restrained mice had to pull a lever for approximately 600 ms to receive a water drop. We found two types of task-related cells and clarified their spatial distribution. Next, we conducted two-photon calcium imaging in the motor cortex during 14 training sessions of the self-initiated lever-pull task. In layer 2/3, the accuracy of neuronal ensemble prediction of lever trajectory remained unchanged globally during the training period. However, in layer 5a, the ensemble prediction accuracy steadily improved and one-third of neurons, including subcortical-projecting neurons, evolved to contribute substantially to ensemble prediction in the late stage of learning. Furthermore, we performed two-photon imaging of dendritic activity and axonal activity in layer 1 during the mouse task performance.
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