Crosstalk between chromosome modification and transcription factors in the differentiation of hematopoietic cells

• Project Area: Molecular mechanisms of cell fate determination in the cells that undergo stepwise differentiation to multiple pathways
• Project/Area Number: 22118002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: KITAMURA Toshio 東京大学, 医科学研究所, 教授 (20282527)
• Co-Investigator(Kenkyū-buntansha): SAKAUE Asako 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (90462689) ; YAMAMOTO Masayuki 東北大学, 医学(系)研究科(研究院), 教授 (50166823)
• Co-Investigator(Renkei-kenkyūsha): ISHIKAWA Fumihiko 独立行政法人理化学研究所, その他部局等, ユニットリーダー (30403918) ; MIYAWAKI Atsushi 独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (80251445)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥285,090,000 (Direct Cost: ¥219,300,000、Indirect Cost: ¥65,790,000); Fiscal Year 2014: ¥48,490,000 (Direct Cost: ¥37,300,000、Indirect Cost: ¥11,190,000); Fiscal Year 2013: ¥50,960,000 (Direct Cost: ¥39,200,000、Indirect Cost: ¥11,760,000); Fiscal Year 2012: ¥51,220,000 (Direct Cost: ¥39,400,000、Indirect Cost: ¥11,820,000); Fiscal Year 2011: ¥52,910,000 (Direct Cost: ¥40,700,000、Indirect Cost: ¥12,210,000); Fiscal Year 2010: ¥81,510,000 (Direct Cost: ¥62,700,000、Indirect Cost: ¥18,810,000)
• Keywords: 細胞分化 / エピジェネティクス / 細胞周期 / 遺伝子 / 核酸 / 発現制御 / 発生・分化 / バイオテクノロジー / Fucci細胞 / 骨髄異形成症候群 / イメージング / トランスジェニックマウス / miRNA

Outline of Final Research Achievements

We have demonstrated that the mutation of ASXL1 an epigenetic factor induces a myelodysplastic syndromes-like disease in mouse using mouse bone marrow transplant models. Concerning the molecular mechanisms, we have identified that suppression of PRC2 functions lead to derepression of HoxA9 and miR125a, playing critical roles in cell transformation and suppression of cell differentiation. In addition, we have identified a series of ASXL1 binding proteins, and investigate the physiological roles of ASXL1 in normal hematopoiesis. We also developed a novel G0 marker which expresses mVenus fluorescent protein only in cells in the G0 phase. We have now established and started the characterization of the transgenic mice expressing this G0 marker in hematopoietic cells.

Research Products

• [Journal Article] The stability of epigenetic factor ASXL1 is regulated through ubiquitination and USP7-mediated deubiquitination. (2015)
  • Author(s): Inoue, D., Nishimura, K., Kozuka-Hata, H., Oyama, M. and Kitamura, T.
  • Journal Title: Leukemia Volume: in press Issue: 11 Pages: 2257-2260
  • DOI: 10.1038/leu.2015.90
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ceramide-CD300f binding suppresses experimental colitis by inhibiting ATP-mediated mast cell activation. (2015)
  • Author(s): Matsukawa T, Izawa K, Isobe M, Takahashi M, Maehara A, Yamanishi Y, Kaitani A, Okumura K, Teshima T, Kitamura T, Kitaura J
  • Journal Title: Gut Volume: 印刷中 Issue: 5 Pages: 1-11
  • DOI: 10.1136/gutjnl-2014-308900
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A C-terminal mutant of CCAAT-enhancer-binding protein α (C/EBPα-C(m)) downregulates Csf1r, a potent accelerator in the progression of acute myeloid leukemia with C/EBPα-C(m). (2015)
  • Author(s): Togami K, Kitaura J, Uchida T, Inoue D, Nishimura K, Kawabata KC, Nagase R, Horikawa S, Izawa K, Fukuyama T, Nakahara F, Oki T, Harada Y, Harada H, Aburatani H, Kitamura T.
  • Journal Title: Exp Hematol. Volume: 43(4) Issue: 4 Pages: 300-308
  • DOI: 10.1016/j.exphem.2014.11.011
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] SETBP1 mutations drive leukemic transformation in ASXL1-mutated MDS. (2015)
  • Author(s): Inoue D, Kitaura J, Matsui H, Hou HA, Chou WC, Nagamachi A, Kawabata KC, Togami K, Nagase R, Horikawa S, Saika M, Micol JB, Hayashi Y, Harada Y, Harada H, Inaba T, Tien HF, Abdel-Wahab O, Kitamura T.
  • Journal Title: Leukemia Volume: 29(4) Issue: 4 Pages: 847-57
  • DOI: 10.1038/leu.2014.301
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Autoimmune susceptible HLA alleles encode unstable proteins. (2015)
  • Author(s): Miyadera, H., Ohashi, J., Lernmark, A., Kitamura, T., and Tokunaga, K.
  • Journal Title: J. Clin. Invest. Volume: 125 Issue: 1 Pages: 275-291
  • DOI: 10.1172/jci74961
  • Peer Reviewed / Open Access
• [Journal Article] The molecular basis of myeloid malignancies (2014)
  • Author(s): Kitamura, T., Inoue, D., Okochi-Watanabe, N., Kato, N., Komeno, Y., Lu, Y., Enomoto, Y., Doki, N., Uchida, T., Kagiyama, Y., Togami, K., Kawabata, K.C., Nagase, R., Horikawa, S., Hayashi, Y., Saika, M., Fukuyama, T., Izawa, K., Oki, T., Nakahara, F., and Kitaura, J.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 90 Issue: 10 Pages: 389-404
  • DOI: 10.2183/pjab.90.389
  • NAID: 130004704862
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hes1 promotes blast crisis in chronic myelogenous leukemia through MMP-9 upregulation in leukemic cells. (2014)
  • Author(s): Nakahara F, Kitaura J, Uchida T, Nishida C, Togami K, Inoue D, Matsukawa T, Kagiyama Y, Enomoto Y, Kawabata KC, Chen-Yi L, Komeno Y, Izawa K, Oki T, Nagae G, Harada Y, Harada H, Otsu M, Aburatani H, Heissig B, Hattori K, Kitamura T.
  • Journal Title: Blood Volume: 123(25) Issue: 25 Pages: 3932-42
  • DOI: 10.1182/blood-2013-01-476747
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ezh2 loss promotes development of myelodysplastic syndrome but attenuates its predisposition to leukemic transformation. (2014)
  • Author(s): Sashida, G., Harada, H., Matsui, H., Oshima, M., Yui, M., Harada, Y., Tanaka, S., Mochizuki-Kashio, M., Wang, C., Saraya, A., Muto, T., Hayasi, Y., Suzuki, K., Nakajima, H., Inaba, T., Koseki, H., Huang, G., Kitamura, T. and Iwama, A.
  • Journal Title: Nat. Commun. Volume: 5 Issue: 1 Pages: 5177-5177
  • DOI: 10.1038/ncomms5177
  • Peer Reviewed / Open Access
• [Journal Article] Nov/ccn3 regulates long-term repopulating activity of murine hematopoietic stem cells via integrin αvβ3 (2014)
  • Author(s): J. Ishihara, et al
  • Journal Title: International Journal of Hematology Volume: (印刷中) Issue: 4 Pages: 393-406
  • DOI: 10.1007/s12185-014-1534-x
  • Peer Reviewed
• [Journal Article] A novel cell-cycle indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. (2014)
  • Author(s): Oki, T., Nishimura, K., Kitaura, J., Togami, K., Maehara, A., Izawa, K., Sakaue-Sawano. A., Niida, A., Miyano, S., Aburatani, H., Kiyonari, H., Miyawaki, A. and Kitamura, T.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 4012-4012
  • DOI: 10.1038/srep04012
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hes1 up-regulation contributes to the development of FIP1L1-PDGFRA-positive leukemia in blast crisis. (2014)
  • Author(s): Uchida T, Kawabata KC, Kitamura T., et al.
  • Journal Title: Exp Hematol. Volume: S0301-472X(14) Issue: 5 Pages: 56-3
  • DOI: 10.1016/j.exphem.2014.01.009
  • Peer Reviewed / Open Access
• [Journal Article] Age-related epigenetic drift in the pathogenesis of MDS and AML. (2014)
  • Author(s): Maegawa, S., Gough, S., Watanabe-Okochi, N., Lu, Y., Zhang, N., Castoro, R.J., Estecio, M.R.H., Jelinek, J., Liang, S., Kitamura, T., Aplan, P., and Issa, J.P.
  • Journal Title: Genome Research. Volume: 24 Issue: 4 Pages: 580-591
  • DOI: 10.1101/gr.157529.113
  • Peer Reviewed
• [Journal Article] Sphingomyelin and ceramide are physiological ligands for human LMIR3/CD300f, inhibiting FceRI-mediated mast cell activation. (2014)
  • Author(s): Izawa, K., Isobe, M., Matsukawa, T., Ito, S., Maehara, A., Takahashi, M., Yamanishi, Y., Kaitani, A., Oki, T., Okumura, K., *Kitamura, T., and *Kitaura, J.
  • Journal Title: J. Allergy. Clin. Immunol. Volume: 133 Issue: 1 Pages: 270-273
  • DOI: 10.1016/j.jaci.2013.08.008
  • Peer Reviewed
• [Journal Article] A promoter-level mammalian expression atlas. (2014)
  • Author(s): Forrest, A.R.R., Kawaji, H., Rehli, M., Baillie, J.K, de Hoon, M.J.L., Haberle, V., Lassmann, T., Kulakovskiy, I.V., Lizio, M., Itoh, M., Andersson, R., Mungall, C. J.,et al.
  • Journal Title: Nature Volume: 507 Issue: 7493 Pages: 462-470
  • DOI: 10.1038/nature13182
  • Peer Reviewed / Open Access
• [Journal Article] A novel cell-cycle indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. (2014)
  • Author(s): Oki, T
  • Journal Title: Scientific Reports Volume: in press
  • Peer Reviewed
• [Journal Article] Age-related epigenetic drift in the pathogenesis of MDS and AML. (2014)
  • Author(s): Maegawa, S
  • Journal Title: Genome Research Volume: in press
  • Peer Reviewed
• [Journal Article] Ezh2 loss promotes development of myelodysplastic syndrome but attenuates its predisposition to leukemic transformation. (2014)
  • Author(s): Sashida, G
  • Journal Title: J. Exp. Med. Volume: in press
  • Peer Reviewed
• [Journal Article] Plzf drives MLL-fusion-mediated leukemogenesis specifically in long-term hematopoietic stem cells. (2013)
  • Author(s): Ono, R
  • Journal Title: Blood Volume: 122 Issue: 7 Pages: 1271-1283
  • DOI: 10.1182/blood-2012-09-456665
  • Peer Reviewed
• [Journal Article] Induction of Human Cardiomyocyte-like Cells from Fibroblasts by Defined Factors. (2013)
  • Author(s): Wada R, Muraoka N, Inagawa K, Tohyama S, et al
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110 Issue: 31 Pages: 12667-72
  • DOI: 10.1073/pnas.1304053110
  • Peer Reviewed
• [Journal Article] Myelodysplastic syndromes are induced by histone methylation-altering ASXL1 mutations. (2013)
  • Author(s): Inoue, D
  • Journal Title: J. Clin. Invest. Volume: 123 Issue: 11 Pages: 4627-4640
  • DOI: 10.1172/jci70739
  • Peer Reviewed
• [Journal Article] APCCDH1 targets MgcRacGAP for destruction in the late M phase. (2013)
  • Author(s): Nishimura, K
  • Journal Title: PLOS One Volume: 8 Issue: 5 Pages: e63001-e63001
  • DOI: 10.1371/journal.pone.0063001
  • Peer Reviewed
• [Journal Article] RUNX1/AML1 mutant collaborates with BMI1 overexpression in tnedevelopment of human and murine myelodysplastic syndromes. (2013)
  • Author(s): 原田結花、井上大地, 他
  • Journal Title: Blood Volume: 128(3) Issue: 17 Pages: 3434-3446
  • DOI: 10.1182/blood-2012-06-434423
  • Peer Reviewed
• [Journal Article] Genetic and epigenetic alterations in hematopoietic malignancies. (2013)
  • Author(s): Kitamura, T
  • Journal Title: Int. J. Hematol. Volume: 97 Issue: 2 Pages: 163-164
  • DOI: 10.1007/s12185-012-1235-2
• [Journal Article] Junctional Rab13-binding protein (JRAB) re requires cell spreading via filamins. (2013)
  • Author(s): Sakane, A
  • Journal Title: Genes Cells Volume: 18 Issue: 9 Pages: 810-822
  • DOI: 10.1111/gtc.12078
  • Peer Reviewed
• [Journal Article] Upregulation of CD200R1 in lineage-negative leukemic cells is characteristic of AML1-ETO-positive leukemia in mice. (2012)
  • Author(s): Kagiyama Y, Kitaura J, Togami K, Uchida T, Inoue D, Matsukawa T, Izawa K, Kawabata KC, Komeno Y, Oki T, Nakahara F, Sato K, Aburatani H, Kitamura T.
  • Journal Title: Int J Hematol. Volume: 96(5) Issue: 5 Pages: 638-48
  • DOI: 10.1007/s12185-012-1207-6
  • Peer Reviewed
• [Journal Article] The receptor LMIR3 negatively regulates mast cell activation and allergic responses by binding to extracellular ceramide. (2012)
  • Author(s): Izawa K, Yamanishi Y, Maehara A, Takahashi M, Isobe M, Ito S, Kaitani A, Matsukawa T, Matsuoka T, Nakahara F, Oki T, Kiyonari H, Abe T, 0kumura K, Kitamura T, Kitaura J.
  • Journal Title: Immunity Volume: 16;37(5) Issue: 5 Pages: 827-39
  • DOI: 10.1016/j.immuni.2012.08.018
  • Peer Reviewed
• [Journal Article] A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis. (2012)
  • Author(s): Yoshinori Yamanishi
  • Journal Title: Journal of Immunology Volume: 189 Issue: 4 Pages: 1773-1779
  • DOI: 10.4049/jimmunol.1201139
  • Peer Reviewed
• [Journal Article] HDACI-induced thrombocytopenia is caused by its unexpected target. (2012)
  • Author(s): 北村俊雄、井上大地
  • Journal Title: Experimental hematology Volume: 40(9) Issue: 9 Pages: 695-697
  • DOI: 10.1016/j.exphem.2012.07.001
  • Peer Reviewed
• [Journal Article] Fyn is not essential for Bcr-Ab1-induced leukemogenesis in mouse bone marrow transplantation models (2012)
  • Author(s): Doki N, Kitaura J, Inoue D, Kato N, Kagiyama Y, Uchida T, Togami K, Isobe M, Ito S, Maehara A, Izawa K, Oki T, Harada Y, Nakahara F, Harada H, Kitamura T
  • Journal Title: Int J Hematol Volume: 95(2) Issue: 2 Pages: 167-175
  • DOI: 10.1007/s12185-011-0994-5
  • Peer Reviewed
• [Journal Article] Aberrant expression of RasGRP1 cooperates with gain-of-function NOTCH1 mutations in T-cell leukemogenesis (2012)
  • Author(s): Oki T, Kitaura J, Watanabe-Okochi N, Nishimura K, Maehara A, Uchida T, Komeno Y, Nakahara F, Harada Y, Sonoki T, Harada H, Kitamura T
  • Journal Title: Leukemia Volume: (in press) Issue: 5 Pages: 1038-1045
  • DOI: 10.1038/leu.2011.328
  • Peer Reviewed
• [Journal Article] TSC-22 is a negative-feedback regulator of Ras/Raf signaling: Implications for tumorigenesis. (2012)
  • Author(s): Nakamura, M
  • Journal Title: Cancer Science Volume: 103 Issue: 1 Pages: 26-33
  • DOI: 10.1111/j.1349-7006.2011.02108.x
  • Peer Reviewed
• [Journal Article] TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. (2012)
  • Author(s): Delhase, M
  • Journal Title: Proc Natl Acad Sci Volume: 109 Issue: 11 Pages: 177-86
  • DOI: 10.1073/pnas.1201576109
  • Peer Reviewed
• [Journal Article] Development and Function of Invariant Natural Killer T cells Producing TH2- and TH17-cytokines (2012)
  • Author(s): Watarai H, Sekine-Kondo E, Shigeura T, Motomura Y, Yasuda T, Satoh R, Yoshida H, Kubo M, Kawamoto H, Koseki H, Taniguchi M
  • Journal Title: PLoS Biol Volume: 10 Issue: 2 Pages: e1001255-e1001255
  • DOI: 10.1371/journal.pbio.1001255
  • Peer Reviewed
• [Journal Article] Survival and death signals can predict tumor response to therapy after oncogene inactivation (2012)
  • Author(s): Tran, P.T
  • Journal Title: Science Translational Medicine Volume: (in press) Issue: 103
  • DOI: 10.1126/scitranslmed.3002018
  • Peer Reviewed
• [Journal Article] Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression cloning (2012)
  • Author(s): Shibata-Minoshima, F
  • Journal Title: Int J Oncology Volume: 40 Pages: 93-98
  • DOI: 10.3892/ijo.2011.1173
  • Peer Reviewed
• [Journal Article] Plasma membrane-associated sialidase (NEU3) regulates progression of prostate cancer to androgen-independent growth through modulation of androgen receptor signaling (2012)
  • Author(s): Kawamura, S., et al
  • Journal Title: Cell Death Differ Volume: 19 Issue: 1 Pages: 170-179
  • DOI: 10.1038/cdd.2011.83
  • Peer Reviewed
• [Journal Article] Mature erythrocyte membrane homeostasis is compromised by loss of the GATA1-FOG1 interaction (2012)
  • Author(s): Hasegawa A, Shimizu R, Mohandas N, Yamamoto M
  • Journal Title: Blood Volume: 119(11) Issue: 11 Pages: 2615-2623
  • DOI: 10.1182/blood-2011-09-382473
  • Peer Reviewed
• [Journal Article] Em/miR-125b transgenic mice develop lethal B-cell malignancies (2011)
  • Author(s): Enomoto, Y
  • Journal Title: Leukemia Volume: 25 Issue: 12 Pages: 1849-1856
  • DOI: 10.1038/leu.2011.166
  • Peer Reviewed
• [Journal Article] Evi1 represses PTEN expression and activates PI3K/AKT/mTOR via interactions with polycomb proteins (2011)
  • Author(s): Yoshimi Akihide
  • Journal Title: Blood Volume: 117 Issue: 13 Pages: 3617-28
  • DOI: 10.1182/blood-2009-12-261602
  • Peer Reviewed
• [Journal Article] Two types of C/EBPa mutations play distinct roles in leukemogenesis : Lessons from clinical data and BMT models (2011)
  • Author(s): Kato, N
  • Journal Title: Blood Volume: 117 Issue: 1 Pages: 221-233
  • DOI: 10.1182/blood-2010-02-270181
  • Peer Reviewed
• [Journal Article] Isolation and Characterization of Renal Erythropoietin-Producing Cells from Genetically Produced Anemia Mice (2011)
  • Author(s): Pan X, Suzuki N, Hirano I, Yamazaki S, Minegishi N, Yamamoto M
  • Journal Title: PLoS ONE Volume: 6 Issue: 10 Pages: 25839-25839
  • DOI: 10.1371/journal.pone.0025839
  • Peer Reviewed
• [Journal Article] Dysfunction of fibroblasts of extra-renal origin underlies renal fibrosis and renal anemia in mice (2011)
  • Author(s): Asada N, Takase M, Nakamura J, Oguchi A, Asada M, Suzuki N, Yamamura K-i, Nagoshi N, Shibata S, Rao TN, Fehling HJ, Fukatsu A, Minegishi N, Kita T, Kimura T, Okano H, Yamamoto M, Yanagita M
  • Journal Title: J Clin Invest Volume: 121 Issue: 10 Pages: 3981-3990
  • DOI: 10.1172/jci57301
  • Peer Reviewed
• [Journal Article] Specific contribution of the erythropoietin gene 3' enhancer to hepatic erythropoiesis after late embryonic stages (2011)
  • Author(s): Suzuki N, Obara N, Pan X, Watanabe M, Jishage K-i, Minegishi N, Yamamoto M
  • Journal Title: Mol Cell Biol Volume: 31 Issue: 18 Pages: 3896-3905
  • DOI: 10.1128/mcb.05463-11
  • Peer Reviewed
• [Journal Article] Heme regulates B-cell differentiation, antibody class switch, and heme oxygenase-1 expression in B cells as a ligand of Bach2 (2011)
  • Author(s): Watanabe-Matsui M
  • Journal Title: Blood Volume: 117 Issue: 20 Pages: 5438-48
  • DOI: 10.1182/blood-2010-07-296483
  • Peer Reviewed
• [Journal Article] Scale : a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain (2011)
  • Author(s): Hiroshi Hama
  • Journal Title: Nature Neuroscience Volume: 14 Issue: 11 Pages: 1481-1488
  • DOI: 10.1038/nn.2928
  • Peer Reviewed
• [Journal Article] Proteins on the move : insights gained from fluorescent protein technologies (2011)
  • Author(s): Miyawaki A
  • Journal Title: Nat.Rev.Mol.Cell Biol. Volume: 12 Issue: 10 Pages: 656-668
  • DOI: 10.1038/nrm3199
  • Peer Reviewed
• [Journal Article] Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer (2011)
  • Author(s): Miyawaki A
  • Journal Title: Annu Rev Biochem. Volume: 80 Issue: 1 Pages: 357-373
  • DOI: 10.1146/annurev-biochem-072909-094736
  • Peer Reviewed
• [Presentation] レトロウイルスベクターを利用した高効率遺伝子導入法とその応用 (2015)
  • Author(s): 北村俊雄
  • Organizer: 第１４回再生医療学会 シンポジウム
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2015-03-21
  • Invited
• [Presentation] Molecular basis by which mutations of epigenetic factors induce MDS-like symptons. (2015)
  • Author(s): K. Kawabata, D. Inoue and T. Kitamura.
  • Organizer: 日米造血器腫瘍
  • Place of Presentation: ハワイ島・米国
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] MDS induced by ASXL1 mutations and its leukemic transformation by an additional SETBP1 mutation (2015)
  • Author(s): D. Inoue, H. Matsui, T. Ochiya, F. Thol, M. Heuser, H-A How, W.C. Chou, J. Kitaura, R. Levine, H-F. Tien, O. Abdel-Wahab, T. Kitamura.
  • Organizer: キーストンシンポジウム
  • Place of Presentation: キーストン・米国
  • Year and Date: 2015-01-25 – 2015-01-30
• [Presentation] Molecular mechanisms of MDS and MDS/AML pathogenesis induced by ASXL1 and ASXL1/SETBP1 mutations. (2014)
  • Author(s): D. Inoue, T. Kitamura.
  • Organizer: 第４３回日本免疫学会
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2014-12-10 – 2014-12-12
  • Invited
• [Presentation] MHC-peptide interaction analysis by the cell-surface expression assay (2014)
  • Author(s): H.Miyadera, T.Kitamura, K.Tokunaga.
  • Organizer: 第４３回日本免疫学会
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] LMIR3/CD300f deficiency aggravates DSS-induced colitis (2014)
  • Author(s): T.Matsukawa, K.Izawa, M.Isobe, M.Takahashi, A.Maehara, A.Kaitani, K.Okumura, T.Kitamura, J.Kitaura.
  • Organizer: 第４３回日本免疫学会
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Secreted small RNAs regulate “inflammatory niche” and development of tumors. (2014)
  • Author(s): .N.Yamakawa, K.Imadome, K.Hozumi, K.Ando, T.Kitamura, A.Kotani.
  • Organizer: 第４３回日本免疫学会
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Disrupting ceramide-LMIR3 interaction prevents bacterial sepsis by stimulating neutrophil recruitment. (2014)
  • Author(s): K.Izawa, M.Isobe, T.Matsukawa, A.Maehara, M.Takahashi, A.Kaitani, K.Okumura, T.Kitamura, J.Kitaura.『Disrupting ceramide-LMIR3 interaction prevents bacterial sepsis by stimulating neutrophil recruitment.
  • Organizer: 第４３回日本免疫学会
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] SETBP1 Mutations Drive Leukemic Transformation in ASXL1-Mutated MDS (2014)
  • Author(s): D.Inoue, H.Matsui, HA.Hou, WC.Chou, A.Nagamachi, J.Kitaura, K.Kawabata, K.Togami, R.Nagase, S.Horikawa, M.Saika, JB.Micol, Y.Hayashi, Y.Harada, HF.Tien, O.Abdel-Wahab, T.Kitamura.
  • Organizer: The 56th American Society of Hematology annual meeting
  • Place of Presentation: サンフランシスコ・アメリカ
  • Year and Date: 2014-12-08
• [Presentation] MDS induced by ASXL1 mutations and its leukemic transformation by an additional SETBP1 mutation. (2014)
  • Author(s): D. Inoue and T. Kitamura.
  • Organizer: 日仏がんワークショップ
  • Place of Presentation: 京都・関西セミナーハウス
  • Year and Date: 2014-11-19 – 2014-11-21
• [Presentation] 骨髄系造血器腫瘍発症の分子機構 (2014)
  • Author(s): 北村俊雄
  • Organizer: 第７６回日本血液学会
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] C-terminal truncation type of RUNX1 mutants induce MDS/AML via gain-of-function mechanisms (2014)
  • Author(s): H.Harada, Y.Harada, D.Inoue, Y.Ding, G.Sashida, A.Iwama, H.Nakajima, J.Tanaka, N.Komatsu, T.Kitamura.
  • Organizer: 第７６回日本血液学会
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] Dominant negative EZH2 promotes myeloid tumorigenesis in mouse BMT model. (2014)
  • Author(s): K.Kawabata, D.Inoue, J.Kitaura, T.Kitamura.
  • Organizer: 第７６回日本血液学会
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] ETBP1 mutations drive leukemic transformation in ASXL1-mutated MDS (2014)
  • Author(s): D.Inoue, J.Kitaura, K.Kawabata, H.Matsui, K.Togami, R.Nagase, S.Horikawa, T.Kitamura.
  • Organizer: 第７６回日本血液学会
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] ASXL1変異によるMDS・AML発症機構の解析 (2014)
  • Author(s): 井上大地
  • Organizer: 第130回小児血液腫瘍免疫懇話会
  • Place of Presentation: 東京・東京医科歯科大学
  • Year and Date: 2014-10-17
• [Presentation] Molecular roles of ASXL1 mutations in inducing myelodysplastic syndromes. (2014)
  • Author(s): 北村俊雄
  • Organizer: 日本癌学会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] SETBP1 Mutations Drive Leukemic Transformation in ASXL1-Mutated MDS. (2014)
  • Author(s): D.Inoue.
  • Organizer: 43rd ISEH annual meeting
  • Place of Presentation: モントリオール・カナダ
  • Year and Date: 2014-08-23
• [Presentation] Modeling the hematological malignancies in mice. (2014)
  • Author(s): T. Kitamura.
  • Organizer: 43rd ISEH annual meeting
  • Place of Presentation: モントリオール・カナダ
  • Year and Date: 2014-08-21 – 2014-08-24
  • Invited
• [Presentation] ASXL1変異によるMDS発症機構の解明 (2014)
  • Author(s): 井上大地・北村俊雄
  • Organizer: 第１０回麒麟塾
  • Place of Presentation: 東京 コクヨホール
  • Year and Date: 2014-07-10
• [Presentation] C-terminal-truncating ASXL1 mutations induce MDS via repression of PRC2 function (2014)
  • Author(s): 井上大地・北村俊雄
  • Organizer: 平成26年度医科学研究所研究成果発表会
  • Place of Presentation: 東京・東京大学医科学研究所
  • Year and Date: 2014-05-31
• [Presentation] MDS induced by ASXL1 mutations and its leukemic transformation by an additional SETBP1 mutation. (2014)
  • Author(s): T. Kitamura, D. Inoue, and J. Kitaura.
  • Organizer: Molecular Aspects of Myeloid Stem Cell Development and Leukemia.
  • Place of Presentation: シンシナティ・米国
  • Year and Date: 2014-05-04 – 2014-05-07
• [Presentation] Modeling hematological malignancies. (2014)
  • Author(s): T. Kitamura.
  • Organizer: Hematopoiesis meeting.
  • Place of Presentation: ルンド・スウェーデン
  • Year and Date: 2014-04-24 – 2014-04-25
  • Invited
• [Presentation] ASXL1 mutations induces an MDS-like disease in mice via derepression of HoxA9 and miR125a (2013)
  • Author(s): 北村俊雄
  • Organizer: 日仏がん会議（French Japanese Cancer Meeting）
  • Place of Presentation: フランス・トゥールーズ
  • Invited
• [Presentation] Elucidating the control mechanisms of Microcap expression (2012)
  • Author(s): 西村耕太郎
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡（福岡）
  • Year and Date: 2012-12-12
• [Presentation] Phosphatidylethanolamine is a possible ligand for human CD300c which delivers an FcRg-dependent activating signal in mast cells and monocytes. (2012)
  • Author(s): 高橋まり子
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際展示場（兵庫）
  • Year and Date: 2012-12-06
• [Presentation] Extracellular ceramide is a ligand for LMIR3 which negatively regulates mast-cell-dependent allergic responses. (2012)
  • Author(s): 伊沢久未
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際展示場（兵庫）
  • Year and Date: 2012-12-06
• [Presentation] MLL fusion genes transform hematopoietic stem cells through an aberrant self-renewal program by Plzf. (2012)
  • Author(s): Ryoichi Ono
  • Organizer: 第74回日本血液学会
  • Place of Presentation: 京都国際会館（京都）
  • Year and Date: 2012-10-20
• [Presentation] NS-018 suppresses myeloma cell growthand osteoclast formation by inhibiting JAK2 and Src pathways. (2012)
  • Author(s): Ayumi Kodama
  • Organizer: 第74回日本血液学会
  • Place of Presentation: 京都国際会館（京都）
  • Year and Date: 2012-10-20
• [Presentation] RasGRP1 and its roles in T-ALL. (2012)
  • Author(s): 沖俊彦
  • Organizer: 第74回日本血液学会
  • Place of Presentation: 京都国際会館（京都）
  • Year and Date: 2012-10-20
• [Presentation] Leukemogenesis induced by C-terminal mutations in the basic leucine zipper domain of C/EBPα. (2012)
  • Author(s): 戸上勝仁
  • Organizer: 第74回日本血液学会
  • Place of Presentation: 京都国際会館（京都）
  • Year and Date: 2012-10-20
• [Presentation] Molecular Mechanisms of blast crisis in chronic myelogenous leukemia caused by upregulation of Hes1. (2012)
  • Author(s): 中原史雄
  • Organizer: 第74回日本血液学会
  • Place of Presentation: 京都国際会館（京都）
  • Year and Date: 2012-10-20
• [Presentation] An active mutant of c-Kit collaborates with AML1-ETO in inducing AML in a mouse BMT model. (2012)
  • Author(s): 鍵山侑希
  • Organizer: 74回日本血液学会
  • Place of Presentation: 京都国際会館（京都）
  • Year and Date: 2012-10-19
• [Presentation] The roles of Hes1 in the myeloid leukemogenesis. (2012)
  • Author(s): 内田智之
  • Organizer: 第74回日本血液学会
  • Place of Presentation: 京都国際会館（京都）
  • Year and Date: 2012-10-19
• [Presentation] LMIR3/CD300f negatively regulates mast cell-dependent allergic responses. (2012)
  • Author(s): 伊沢久未
  • Organizer: The International Symposium Allergy and Inflammation
  • Place of Presentation: 韓国 ソウル
  • Year and Date: 2012-10-13
  • Invited
• [Presentation] RUNX1/AML1 Mutants collaborate with BMI1 in the development of myelodysplastic syndromes. (2012)
  • Author(s): Hironori Harada
  • Organizer: ISEH 41st Annual Scientific Meeting/Experimental Hematology
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2012-08-23
• [Presentation] 細胞周期制御因子MgcRacGAPの新規発現調節機構 (2012)
  • Author(s): 西村耕太郎
  • Organizer: 第12回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学山上会館（東京）
  • Year and Date: 2012-06-30
• [Presentation] Two forms of C/EBPa mutants collaborate in leukemogenesis. (2012)
  • Author(s): Toshio Kitamura
  • Organizer: 9th International Workshop on Molecular Aspects of Myeloid Stem Cell Development and Leukemia,
  • Place of Presentation: Cincinnati,USA
  • Year and Date: 2012-05-06
• [Presentation] T細胞白血化におけるNOTCH1の新しい共同因子,RasGRP1 (2012)
  • Author(s): 沖俊彦
  • Organizer: 第16回造血器腫瘍研究会
  • Place of Presentation: 東京国立がんセンター研究所
  • Year and Date: 2012-01-28
• [Presentation] 細胞周期制御因子MgcRacGAPの新規発現調節機構 (2011)
  • Author(s): 西村耕太郎
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] Nov/CCN3 Enhances Long-Term Repopulating Activity of Mouse Hematopoietic Stem Cells Via Intergin β3 Signaling Collaborating with Thrombopoietin (2011)
  • Author(s): Jun Ishihara
  • Organizer: American Society of Hematology Fifty-third annual meeting
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2011-12-10
• [Presentation] Dual Inhibition of the STAT3 and Src Pathways by N-018, a JAK2 and Src-Family Kinase Inhibitor, Reduces Myeloma Cell Proliferation and Adhesion and Suppresses Osteoclast Formation (2011)
  • Author(s): Ayumi Kodama
  • Organizer: American Society of Hematology Fifty-third annual meeting
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2011-12-10
• [Presentation] LMIR3ノックアウトマウスにおけるアレルギー反応の亢進 (2011)
  • Author(s): 伊沢久未
  • Organizer: 日本免疫学会
  • Place of Presentation: 千葉幕張メッセ
  • Year and Date: 2011-11-29
• [Presentation] CD300/LMIRファミリーとマスト細胞 (2011)
  • Author(s): 北浦次郎
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京グランドプリンスホテル新高輪
  • Year and Date: 2011-11-11
• [Presentation] Molecular basis of leukemia, MPN and MDS (2011)
  • Author(s): 北村俊雄
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 愛知名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] Molecular mechanisms of blast crisis in chronic myelogenous leukemia (2011)
  • Author(s): 中原史雄
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 愛知名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] LMIR5/CD300bのリガンド(TIM1)同定と(腎虚血における)機能解析 (2011)
  • Author(s): 北浦次郎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国立京都国際会館
  • Year and Date: 2011-09-21
• [Presentation] Two types of C/EBPa mutations collaborate in inducing leukemia in a mouse BMT model (2011)
  • Author(s): 北村俊雄
  • Organizer: 第25回IACRLRD
  • Place of Presentation: 東京大学本郷キャンパス(招待講演)
  • Year and Date: 2011-09-15
• [Presentation] The Roles of RasGRPs in Hematological Malignancies (2011)
  • Author(s): Toshihiko Oki
  • Organizer: 40th Annual Scientific Meeting of International Society of Experimental Hematology
  • Place of Presentation: Westin Bavshore Hotel, Vancouber, Canada
  • Year and Date: 2011-08-25
• [Presentation] Animal models for AML, MDS and MPN (2011)
  • Author(s): 北村俊雄
  • Organizer: 日本遺伝子治療学会
  • Place of Presentation: 九州大学百年講堂
  • Year and Date: 2011-07-14
• [Presentation] Lessons from mouse models for myelodysplastic syndromes/overt leukemia and chronic myelogeneous leukemia in blast crisis : Insertional mutagenesis induced Evi-1 expression but not Hes1 expression (2011)
  • Author(s): 北村俊雄
  • Organizer: 二国間交流事業セミナー
  • Place of Presentation: 九州大学百年講堂(招待講演)
  • Year and Date: 2011-07-14
• [Presentation] Two types of C/EBPa mutations play distinct roles and collaborate in inducing leukemia (2011)
  • Author(s): 北村俊雄
  • Organizer: International Scientific Coordination Network (ISCN) "FRANCE-JAPAN-CANCER"
  • Place of Presentation: フランスモンペリエ(招待講演)
• [Presentation] C-terminal-truncating ASXL1 mutations induce MDS via repression of PRC2 function
  • Author(s): 井上大地
  • Organizer: 42nd ISEH Annual Scientific Meeting
  • Place of Presentation: オーストリア・ウィーン
• [Presentation] C-terminal-truncating ASXL1 mutations induce MDS via repression of PRC2 function
  • Author(s): 井上大地
  • Organizer: 20th East Asia Joint Symposium
  • Place of Presentation: 日本・東京
• [Presentation] Molecular basis of hematological malignancies
  • Author(s): 北村俊雄
  • Organizer: 20th East Asia Joint Symposium
  • Place of Presentation: 日本・東京
• [Presentation] C-terminal-truncating ASXL1 mutations induce MDS via repression of PRC2 function
  • Author(s): 井上大地
  • Organizer: The 55th American Society of Hematology annual meeting
  • Place of Presentation: アメリカ・ニューオーリンズ
• [Presentation] C-terminal-truncated ASXL1 induced MDS-like symptoms in mice via derepression of miR-125a and reduced expression of Clec5a/Mdl1
  • Author(s): 井上大地
  • Organizer: The 11th Stem Cell Research Symposium
  • Place of Presentation: 日本・東京
• [Presentation] C-terminal-truncating ASXL1 mutations induce MDS via repression of PRC2 function
  • Author(s): 井上大地
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 日本・札幌
• [Presentation] ASXL1 変異による MDS 発症メカニズムの解析
  • Author(s): 井上大地
  • Organizer: 第19回血液科学セミナー
  • Place of Presentation: 日本・東京
• [Presentation] C-terminal-truncating ASXL1 mutations induce MDS via repression of PRC2 function
  • Author(s): 井上大地
  • Organizer: 第64回幹細胞治療研究フォーラム
  • Place of Presentation: 日本・東京
• [Presentation] ASXL1変異によるMDS発症機構の解明と治療応用
  • Author(s): 井上大地
  • Organizer: 東京大学医科学研究所学友会セミナー
  • Place of Presentation: 日本・東京
• [Presentation] Dominant negative EZH2を用いたマウス骨髄移植モデルの検討
  • Author(s): 川畑公人
  • Organizer: 第18回造血器腫瘍研究会
  • Place of Presentation: 日本・東京