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Strategies for survival and growth of rice plants in response to nitrogen starved conditions

Planned Research

Project AreaIntegrated analysis of strategies for plant survival and growth in response to global environmental changes
Project/Area Number 22119003
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTohoku University

Principal Investigator

YAMAYA Tomoyuki  東北大学, (連合)農学研究科(研究院), 教授 (30144778)

Co-Investigator(Kenkyū-buntansha) KUSANO Miyako  筑波大学, 生命環境系, 教授 (60415148)
Co-Investigator(Renkei-kenkyūsha) HAYAKAWA Toshihiko  東北大学, 大学院農学研究科, 准教授 (60261492)
KOJIMA Soichi  東北大学, 大学院農学研究科, 助教 (30462683)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥74,490,000 (Direct Cost: ¥57,300,000、Indirect Cost: ¥17,190,000)
Fiscal Year 2014: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2013: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2010: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Keywordsイネ / 窒素飢餓 / 生存戦略 / 環境突破力 / 同化代謝バランス / メタボローム / 逆遺伝学 / 環境突破 / 窒素同化代謝 / 窒素饑餓 / 代謝ネットワーク / 窒素情報
Outline of Final Research Achievements

Under nitrogen starved conditions, rice plants reduce their active tiller number in vegetative stage and their spikelet number in reproductive stage. However, molecular mechanisms in these responses are largely not known. We have carried out 1) NH4+-signal perception, 2) assimilation and remobilization of nitrogen, 3) metabolic balance between C and N, and 4) modeling of spikelet-ripening to understand molecular mechanisms of response. We obtained clear results, using reverse genetics approaches together with localization works, that glutamine synthetase (GS)1;2 was important in both the primary assimilation of NH4+ and outgrowth of axillary tiller buds, whereas GS1;1 is important in remobilization of N from senescing organs. NADH-glutamate synthase2 was tightly related to the determination of panicle number. These mutants showed metabolic disorder, indicating that N metabolism is important in normal growth and development of rice. Those results were published in several journals.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (37 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Acknowledgement Compliant: 5 results,  Open Access: 3 results) Presentation (15 results) (of which Invited: 4 results) Book (1 results) Remarks (6 results)

  • [Journal Article] Lack of cytosolic glutamine synthetase1;2 in vascular tissues of axillary buds caused severe reduction in their outgrowth and disorder of metabolic balance in rice seedlings2015

    • Author(s)
      Ohashi, M., Ishiyama, K., Kusano, M., Fukushima, A., Kojima, S., Hanada, A., Kanno, K., Hayakawa, T., Seto, Y., Kyozuka, J., Yamaguchi, S., Yamaya, T.
    • Journal Title

      Plant J.

      Volume: 81 Issue: 2 Pages: 347-356

    • DOI

      10.1111/tpj.12731

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A mathematical model of phloem sucrose transport as a new tool for designing rice panicle structure for high grain yield2015

    • Author(s)
      Seki M, Feugier FG, Song X-J, Ashikari M, Nakamura H, Ishiyama K, Yamaya T, Ikeda M, Kitano H, Satake A
    • Journal Title

      Plant and Cell Physiology

      Volume: 56 Issue: 4 Pages: 605-619

    • DOI

      10.1093/pcp/pcu191

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots2015

    • Author(s)
      Ohashi M, Ishiyama K, Kojima S, Nakano K, Kanno K, Hayakawa T, Yamaya, T
    • Journal Title

      Plant and Cell Physiology

      Volume: 56 Issue: 4 Pages: 769-778

    • DOI

      10.1093/pcp/pcv005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Sulfur-responsive elements in the 3’-non-transcribed intergenic region are essential for the induction of SULFATE TRANSPORTER 2;1 gene expression in Arabidopsis roots under sulfur deficiency2015

    • Author(s)
      Maruyama-Nakashita A, Watanabe-Takahashi A, Inoue E, Yamaya T, Saito K Takahashi H
    • Journal Title

      The Plant Cell

      Volume: in press Issue: 4 Pages: 1279-1296

    • DOI

      10.1105/tpc.114.134908

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Autophagy supports biomass production and nitrogen use efficiency at the vegetative stage in rice2015

    • Author(s)
      Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S, Yamaya T, Kuchitsu K, Makino A, Ishida H
    • Journal Title

      Plant Physiology

      Volume: in press Issue: 1 Pages: 60-73

    • DOI

      10.1104/pp.15.00242

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Evidences supporting distinct functions of three cytosolic glutamine synthetases and two NADH-glutamate synthases in rice2014

    • Author(s)
      Yamaya, T. and Kusano M.
    • Journal Title

      Journal of Experimental Botany

      Volume: in press Issue: 19 Pages: 5519-5525

    • DOI

      10.1093/jxb/eru103

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NADH-dependent glutamate synthase plays a crucial role in assimilating ammonium in Arabidopsis root2014

    • Author(s)
      Konishi, N., Ishiyama, K., Matsuoka, K., Maru, I., Hayakawa, T., Yamaya, T. and Kojima, S.
    • Journal Title

      Physiologia Plantarum

      Volume: in press Issue: 1 Pages: 138-151

    • DOI

      10.1111/ppl.12177

    • NAID

      120005691730

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytosolic glutamine synthetase1;2 is responsible for the primary assimilation of ammonium in rice roots2013

    • Author(s)
      Funayama, K.$, Kojima, S.$, Tabuchi-Kobayashi, M., Sawa, Y., Nakayama, Y., Hayakawa, T. and Yamaya, T.
    • Journal Title

      Plant and Cell Physiology

      Volume: 54 Issue: 6 Pages: 934-943

    • DOI

      10.1093/pcp/pct046

    • NAID

      10031184135

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deciphering starch quality of rice kernels using metabolite profiling and pedigree network analysis2012

    • Author(s)
      Kusano, M.
    • Journal Title

      Molecular Plant

      Volume: 5 Issue: 2 Pages: 442-451

    • DOI

      10.1093/mp/ssr101

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissection of genotype-phenotype associations in rice grains using metabolome quantitative trait loci analysis2012

    • Author(s)
      Matsuda, M.
    • Journal Title

      Plant Journal

      Volume: 70 Issue: 4 Pages: 624-636

    • DOI

      10.1111/j.1365-313x.2012.04903.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolomics data reveal a crucial role of cytosolic glutamine synthetasel;1 in coordinating metabolic balance in rice2011

    • Author(s)
      Kusano, et al
    • Journal Title

      The Plant Journal

      Volume: 66 Issue: 3 Pages: 456-466

    • DOI

      10.1111/j.1365-313x.2011.04506.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondorial DNA.2011

    • Author(s)
      Takahashi, et al.
    • Journal Title

      Plant J.

      Volume: 66 Issue: 3 Pages: 433-442

    • DOI

      10.1111/j.1365-313x.2011.04500.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disruption of a novel NADH-glutamate synthase2 gene caused marked reduction in spike let number of rice2011

    • Author(s)
      田村亘, 小島創一, 豊川絢子, 渡邉英生, 田淵-小林真由美, 早川俊彦, 山谷知行
    • Journal Title

      Frontiers in Plant Science

      Volume: vol.2 Pages: 57-57

    • DOI

      10.3389/fpls.2011.00057

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolomics data reveal a crucial role of cytosolic glutamine synthetase 1;1 in coordinating metabolic balance in rice2011

    • Author(s)
      Kusano, et al.
    • Journal Title

      The Plant Journal

      Volume: (未定 印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping quantitative trait loci controlling root length in rice seedlings grown with low or sufficient NH_4^+ supply using backcross recombinant lines derived from a cross between Oryza sativa L.and Oryza glaberrima Steud.2011

    • Author(s)
      Obara, et al.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: (未定 印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Stimulation of axillary buds elongation by metabolite and cytokinin in rice2015

    • Author(s)
      大橋美和、石山敬貴、草野都、福島敦史、小島美紀子、小島創一、早川俊彦、榊原均、山谷知行
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナ根で発現する遺伝子群のアンモニア応答に必要な領域の特定2015

    • Author(s)
      小西範幸,石山敬貴,丸山葵、斉藤雅英、丸郁美,早川俊彦,山谷知行,小島創一
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] イネの根におけるAS分子種の生理的な役割と制御機構の解明2014

    • Author(s)
      大橋美和、石山敬貴、小島創一、早川俊彦、山谷知行
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高親和型尿素輸送担体が破壊されたイネの収量の解析2014

    • Author(s)
      藤田貴之、石山敬貴、早川俊彦、山谷知行、小島創一
    • Organizer
      第32回日本植物細胞分子生物学会盛岡大会・シンポジウム
    • Place of Presentation
      盛岡市 アイーナ
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] イネ高親和型アンモニウムトランスポーター1;3の逆遺伝学的な解析2014

    • Author(s)
      藤田貴之、石塚和、石山敬貴、早川俊彦、山谷知行、山口淳二、小島創一
    • Organizer
      日本土壌肥料学会東北支部会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-07-07 – 2014-07-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] イネ維管束ネットワーク上のショ糖転流と顆粒成長モデル2014

    • Author(s)
      佐竹暁子,関元秀,Francois Feugier,池田真由子,北野英己,Xian-Jun Song,芦苅基行,中村巴瑠花,石山敬貴,山谷知行
    • Organizer
      第55回日本植物生理学会年会シンポジウム
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] イネ根におけるアンモニウム態窒素吸収の負の制御の新理解2014

    • Author(s)
      早川俊彦、山谷知行
    • Organizer
      第55回日本植物生理学会年会シンポジウム
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Strigolactone-independent reduction of tiller number in rice mutant lacking cytosolic glutamine synthetase1;2 or NADH-glutamate synthase12014

    • Author(s)
      Miwa Ohashi,Keiki Ishiyama,Atsushi Hanada,Soichi Kojima,Toshihiko Hayakawa,Yoshiya Seto,Shinjiro Yamaguchi,Tomoyuki Yamaya
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナサイトゾル型グルタミン合成酵素1;2は根でアンモニウムによって誘導されアンモニウムを同化する2013

    • Author(s)
      小西範幸 (山谷 知行)
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台(東北大学)
    • Year and Date
      2013-03-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナNADHグルタミン酸合成酵素の根におけるアンモニウム同化の重要性2013

    • Author(s)
      小西範幸 (山谷 知行)
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山(岡山大学)
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナ環境型におけるアンモニウム栄養利用の遺伝的な差異2013

    • Author(s)
      松岡香矢 (山谷 知行)
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山(岡山大学)
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Distinct function of GS1 and NADH-GOGAT isoenzymes in rice2013

    • Author(s)
      Yamaya, T.
    • Organizer
      Second International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Puerto Varas, Chile
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] The study of two cytosolic glutamine synthetase isoforms of rice using reverse genetic, metabolite and transcript profiling approaches and microscopic analysis2013

    • Author(s)
      Kusano, M., Fukushima, A., Funayama, K., Tabuchi-Kobayashi, M., Nishizawa, T., Kobayashi, M., Waakazaki, M., Sato, M., Toyooka, K., Osanai-Kondo, K., Utsumi, Y., Seki, M., Kojima, S., Yamaya, T., Saito, K.
    • Organizer
      Second International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Puerto Varas, Chile
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ご挨拶・PCP表紙コンテスト授賞式(PCP編集長)2012

    • Author(s)
      山谷知行
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Opening Remarks (as a Chief Organizer)2010

    • Author(s)
      Yamaya, T.
    • Organizer
      The 1st International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Inuyama, Japan
    • Related Report
      2010 Annual Research Report
  • [Book] Nitrogen Assimilation in Plants2010

    • Author(s)
      Kojima, et al.
    • Total Pages
      378
    • Publisher
      Research Signpost
    • Related Report
      2010 Annual Research Report
  • [Remarks] 東北大学大学院農学研究科植物細胞生物学

    • URL

      http://www.agri.tohoku.ac.jp/j030100/id0024.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 植物細胞生物学分野

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2013 Annual Research Report
  • [Remarks] 植物細胞生物学分野

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/rellbio/index-j.htm

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/agri-field/025.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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