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Novel Mechanism of Post-transcriptional Regulation in Nutritional Response

Planned Research

Project AreaIntegrated analysis of strategies for plant survival and growth in response to global environmental changes
Project/Area Number 22119006
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionHokkaido University

Principal Investigator

NAITO Satoshi  北海道大学, (連合)農学研究科(研究院), 教授 (20164105)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥94,640,000 (Direct Cost: ¥72,800,000、Indirect Cost: ¥21,840,000)
Fiscal Year 2014: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2013: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2012: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2010: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
KeywordsmRNA分解 / リボソーム / 翻訳停止 / 試験管内翻訳系 / シロイヌナズナ / 試験管内翻
Outline of Final Research Achievements

Cystathionine γ-synthase (CGS) catalyzes the first committed step of methionine biosynthesis in plants. Expression of CGS1 gene that encodes CGS is feedback-regulated, in response to S-adenosyl-L-methonine (SAM), by translation arrest at Ser-94 codon followed by mRNA degradation. Studies using in vitro translation system revealed that nascent peptide of CGS1 takes compact conformation upon translation arrest, and conformation of 28S rRNA and/or interaction between the nascent peptide and rRNA changes. Upon translation arrest, ribosomes are stacked behind the initially arrested ribosome at Ser-94. We revealed that the stacked ribosomes are arranged at nine codon intervals.
During general mRNA degradation, poly(A) shortening is the first and usually the rate-limiting step. In SAM-induced CGS1 mRNA degradation, however, the poly(A) shortening was not observed.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (46 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 4 results,  Open Access: 4 results) Presentation (35 results) (of which Invited: 2 results) Remarks (2 results)

  • [Journal Article] Identification of novel Arabidopsis thaliana upstream open reading frames that control expression of the main coding sequences in a peptide sequence-dependent manner.2015

    • Author(s)
      Ebina I, Takemoto-Tsutsumi M, Watanabe S, Koyama H, Endo Y, Kimata K, Igarashi T, Murakami K, Kudo R, Ohsumi A, Noh AL, Takahashi H, Naito S, Onouchi H
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Issue: 3 Pages: 1562-1576

    • DOI

      10.1093/nar/gkv018

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Polyamine-responsive ribosomal arrest at the stop codon of an upstream open reading frame of the AdoMetDC1 gene triggers nonsense-mediated mRNA decay in Arabidopsis thaliana.2014

    • Author(s)
      Uchiyama-Kadokura N, Murakami K, Takemoto M, Koyanagi N, Murota K, Naito S, Onouchi H
    • Journal Title

      Plant and Cell Physiology

      Volume: 55 Issue: 9 Pages: 1556-1567

    • DOI

      10.1093/pcp/pcu086

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The N-terminal cleavable pre-sequence encoded in the first exon of cystathionine γ-synthase contains two different functional domains for chloroplast targeting and regulation of gene expression.2014

    • Author(s)
      Hagiwara-Komoda Y, Sugiyama T, Yamashita Y, Onouchi H, Naito S
    • Journal Title

      Plant and Cell Physiology

      Volume: 55 Issue: 10 Pages: 1779-1779

    • DOI

      10.1093/pcp/pcu110

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A U-system approach for predicting metabolic behaviors and responses based on an alleged metabolic reaction network.2014

    • Author(s)
      Sriyudthsak K, Sawada Y, Chiba Y, Yamashita Y, Kanaya S, Onouchi H, Fujiwara T, Naito S, Voit EO, Shiraishi F, Hirai MY
    • Journal Title

      BMC Systems Biology

      Volume: 8 Issue: S5

    • DOI

      10.1186/1752-0509-8-s5-s4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ribosomes in a Stacked Array : Elucidation of the Step in Translation Elongation at Which They Are Stalled During S-Adenosyl-L-methionine-Induced Translation Arrest of CGS1 mRNA2014

    • Author(s)
      Yui Yamashita, Yoshitomo Kadokura, Naoyuki Sotta, Toru Fujiwara, Ichigaku Takigawa, Akiko Satake, Hitoshi Onouchi, and Satoshi Naito
    • Journal Title

      Journal of Biological Chemistry

      Volume: (印刷中) Issue: 18 Pages: 12693-12704

    • DOI

      10.1074/jbc.m113.526616

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A halt in poly(A) shortening during <i>S</i>-adenosyl-L-methionine-induced translation arrest in <i>CGS1</i> mRNA of <i>Arabidopsis thaliana</i>2013

    • Author(s)
      Yui Yamashita, Ingrid Lambein, Soko Kobayashi, Hitoshi Onouchi, Yukako Chiba and Satoshi Naito
    • Journal Title

      Genes & Genetic Systems

      Volume: 88 Issue: 4 Pages: 241-249

    • DOI

      10.1266/ggs.88.241

    • NAID

      130003390805

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in mRNA stability associated with cold stress in Arabidopsis cells2013

    • Author(s)
      Chiba Y, Mineta K, Hirai MY, Suzuki S, Kanaya S, Takahashi H, Onouchi H, Yamaguchi J, Naito S
    • Journal Title

      Plant Cell Physiol

      Volume: 54 Issue: 2 Pages: 180-194

    • DOI

      10.1093/pcp/pcs164

    • NAID

      10031145661

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabidopsis cell-free extract, ACE, a new in vitro translation system derived from Arabidopsis callus cultures2011

    • Author(s)
      Murota K
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Pages: 1443-1453

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] S-Adenosyl-L-methionine Induces Compaction of Nascent Peptide Chain inside the Ribosomal Exit Tunnel upon Translation Arrest in Arabidopsis CGS1.2011

    • Author(s)
      尾上典之, 山下由衣, 長尾信宏, 後藤デレック, 尾之内均, 内藤哲
    • Journal Title

      Journal of Biological Chemistry

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナにおける最小uORFを介したホウ素依存的なリボソーム停滞によるmRNA分解・翻訳効率の制御機構2015

    • Author(s)
      田中真幸,反田直之,三輪京子,山下由衣,千葉由佳子,尾之内均,内藤 哲、藤原 徹
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナCGS1遺伝子の翻訳アレストにおける新生ペプチドの収縮とリボソーム出口トンネルの研究2015

    • Author(s)
      大橋悠文,山下由衣,尾上典之,尾之内均,内藤 哲
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温ストレスに応答したmRNA合成と分解の協調的制御2015

    • Author(s)
      荒江星拓,以西史織,峯田克彦,平井優美,鈴木悠也,金谷重彦,山口淳二,内藤 哲,千葉由佳子
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナCGS1 mRNAにおける翻訳アレスとに先立って起こるリボソームの状態変化2014

    • Author(s)
      山下由衣,榊原 知,尾之内均,内藤 哲
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 最小uORF上でのホウ素依存的なリボソーム停滞を介した遺伝子発現制御2014

    • Author(s)
      田中真幸,反田直之,三輪京子,千葉由佳子,尾之内均,内藤 哲、藤原 徹
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温ストレスに応答したmRNA合成と分解の協調的制御2014

    • Author(s)
      千葉由佳子,以西史織,峯田克彦,平井優美,鈴木悠也,金谷重彦,山口淳二,内藤 哲
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Coordinated regulations of mRNA synthesis and degradation during cold acclimation in Arabidopsis cells.2014

    • Author(s)
      Chiba, Y., Isai, S., Mineta, K., Hirai, M.Y., Suzuki, Y., Kanaya, S., Yamaguchi, J., Naito, S.
    • Organizer
      FASEB Science Research Conference, Post-transcriptional Control of Gene Expression: Mechanisms of mRNA Decay
    • Place of Presentation
      Big Sky, Montana, USA
    • Year and Date
      2014-07-06 – 2014-07-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ribosomes stack and are arrested at the early step of translocation upon S-adenosyl-L-methionine-induced translation arrest of Arabidopsis CGS1 mRNA.2014

    • Author(s)
      Yamashita, Y., Kadokura, Y., Sotta, N., Fujiwara, T., Satake, A., Tagigawa, I., Onouchi, H., Naito, S.
    • Organizer
      FASEB Science Research Conference, Post-transcriptional Control of Gene Expression: Mechanisms of mRNA Decay
    • Place of Presentation
      Big Sky, Montana, USA
    • Year and Date
      2014-07-06 – 2014-07-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 環境変化に適応するためのmRNA分解制御2014

    • Author(s)
      鈴木悠也,以西史織,荒江星拓,峰田克彦,平井優美,山口淳二,Pamela J. Green,内藤 哲、千葉由佳子
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学(富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 低温ストレス応答に関わるCBF遺伝子のmRNA分解制御2014

    • Author(s)
      以西史織,峰田克彦,平井優美,鈴木悠也,山口淳二,内藤 哲、千葉由佳子
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学 (富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ホウ素に応答してリボソームは最小uORF,AUGUAAで停止する2014

    • Author(s)
      田中真幸,三輪京子,千葉由佳子,尾之内均、内藤 哲,藤原 徹
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学 (富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] A halt in poly(A) shortening during S-adenosyl-L-methionine-induced translation arrest in CGS1 mRNA of Arabidopsis.2014

    • Author(s)
      Yui Yamashita, Ingrid Lambein, Soko Kobayashi, Hitoshi Onouchi, Yukako Chjiba, Satoshi Naito
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学 (富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナCGS1 mRNAにおけるリボソームの停滞と新生ペプチドの収縮2013

    • Author(s)
      内藤 哲
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] 栄養センサーとしてのリボソーム:シロイヌナズナにおけるメチオニン生合成のフィードバック制御2013

    • Author(s)
      内藤 哲
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナCGS1 mRNAの翻訳停止におけるリボソーム出口トンネルの解析2013

    • Author(s)
      大橋悠文,尾上典之,尾之内均、内藤 哲
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県民文化会館 (松山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] S-アデノシルメチオニンはシロイヌナズナCGS1 mRNAにおける翻訳アレストに先立って新生ペプチドの収縮を引き起こす2013

    • Author(s)
      山下由衣,尾之内均,内藤 哲
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県民文化会館 (松山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナホウ素輸送隊遺伝子のホウ素栄養条件に応じた発現はshort uORFを介して起こる2013

    • Author(s)
      田中真幸,千葉由佳子,三輪京子,尾之内均、内藤 哲,藤原 徹
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県民文化会館 (松山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナCGS1 mRNAの翻訳制御におけるリボソームの停滞と新生ペプチドの収縮2013

    • Author(s)
      山下由衣,尾之内均,内藤 哲
    • Organizer
      第31回日本植物細胞分子生物学会大会
    • Place of Presentation
      北海道大学 (札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナCGS1遺伝子の転写後制御機構におけるリボソーム出口トンネルの研究2013

    • Author(s)
      大橋悠文,尾之内均、内藤 哲
    • Organizer
      第31回日本植物細胞分子生物学会大会
    • Place of Presentation
      北海道大学 (札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] シロイヌナズナCGS1遺伝子の転写後制御にかかわるヌクレアーゼ因子同定に向けた生化学的解析2012

    • Author(s)
      内藤 哲
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2012-12-11
    • Related Report
      2012 Annual Research Report
  • [Presentation] メチオニン生合成制御を基軸とした新規転写後制御機構の研究2012

    • Author(s)
      内藤 哲
    • Organizer
      第30回日本植物細胞分子生物学会
    • Place of Presentation
      奈良先端科学技術大学院大学(生駒市)
    • Year and Date
      2012-08-03
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナCGS1 mRNAにおける複数のリボソームの停滞:キューろマイシンを用いた翻訳伸長停止段階の特定2012

    • Author(s)
      内藤 哲
    • Organizer
      第14回日本RNA学会年会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2012-07-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] Ribosome as a nutrient sensor, Mechanism of feedback regulation of methionine biosynthesis in Arabidopsis2012

    • Author(s)
      Naito, S.
    • Organizer
      Plant RNA Workshop
    • Place of Presentation
      Hofburg Imperial Palace, Vienna (Austria)
    • Year and Date
      2012-07-08
    • Related Report
      2012 Annual Research Report
  • [Presentation] Autonomously arrested ribosome and those stacked behind it are in different states during translation elongation arrest induced by S-adenosyl-L- methionine in Arabidopsis CGS1 mRNA2012

    • Author(s)
      Naito, S.
    • Organizer
      23rd International Conference on Arabidopsis Research
    • Place of Presentation
      Gregor Mendel Institute, Vienna (Austria)
    • Year and Date
      2012-07-03
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナのホウ素輸送体NIP5;1遺伝子のホウ素に応答したmRNA蓄積の制御機構2012

    • Author(s)
      田中真幸
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナ細胞抽出液を用いたシロイヌナズナのシスタチオニンγ-シンターゼmRNAの分解中間体の解析2012

    • Author(s)
      室田勝功
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナCGS1 mRNAにおける複数のリボソームの停滞とその部分翻訳産物の解析2012

    • Author(s)
      山下由衣
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低温ストレス応答に関連したmRNA分解制御2012

    • Author(s)
      千葉由佳子
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都市)
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナCGS1 mRNAにおけるリボソームの追突によって生じる複数の部分翻訳産物の解析2011

    • Author(s)
      山下由衣
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Nascent peptide-mediated translation arrest of Arabidopsis CGS1 mRNA that occurs in response to S-adenosyl-L-methionine2011

    • Author(s)
      Onoue N
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)(招待講演)
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] S-アデノシルメチオニンに応答したシロイヌナズナCGS1 mRNAにおける翻訳伸長の一時停止とリボソーム内新生ペプチドの収縮構造2011

    • Author(s)
      山下由衣
    • Organizer
      第29回日本植物細胞分子生物学会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Translation arrest and CGS1 nascent peptide compaction in Arabidopsis2011

    • Author(s)
      Yamashita Y
    • Organizer
      16th Annual Meeting of the RNA Society
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Conformation changes of the ribosome-nascent peptide complex that occurs during S-adenosyl-L-methionine-induced translation arrest of Arabidopsis CGS1 mRNA2011

    • Author(s)
      Onoue N
    • Organizer
      16th Annual Meeting of the RNA Society
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2011-06-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] S-アデノシルメチオニンはシロイヌナズナCGS1新生ペプチドに縮んだ構造をとらせる2011

    • Author(s)
      山下由衣, 尾上典之, 尾之内均, 内藤哲
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      年会(仙台,東北大学)は地震により中止となったが,要旨集の公開をもって成立(学会HP http://www.jspp.org/参照)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] CGS1遺伝子発現の転写後自己制御機構:翻訳アレストに関与する配列の解析2010

    • Author(s)
      中西健彦, 古家由理, 尾上典之, 尾之内均, 内藤哲
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸,神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Remarks] リボソームによる細胞内環境の検知と遺伝子発現制御

    • URL

      http://www.agr.hokudai.ac.jp/arabi/research.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 新生ペプチドによる翻訳停止と共役したmRNA分解

    • URL

      http://arabi4.agr.hokudai.ac.jp/Research/CGS1/CGS1.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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