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Molecular mechanisms underlying phase transition of cell proliferation during root growth

Planned Research

Project AreaIntegrated analysis of strategies for plant survival and growth in response to global environmental changes
Project/Area Number 22119009
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNara Institute of Science and Technology

Principal Investigator

UMEDA MASAAKI  奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80221810)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥107,900,000 (Direct Cost: ¥83,000,000、Indirect Cost: ¥24,900,000)
Fiscal Year 2014: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2012: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2011: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2010: ¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Keywords細胞分裂 / 細胞伸長 / 細胞周期 / 根 / 植物ホルモン / DNA損傷 / 転写因子 / イメージング / ストレス / 発現制御 / 発生・分化 / シグナル伝達 / 細胞死 / ゲノム / 植物 / 成長 / エンドサイクル / 環境
Outline of Final Research Achievements

Root growth is affected by various underground conditions. The growth rate is determined by cell division in the root apex and transition from cell division to cell elongation. In this project, we studied underlying mechanisms to uncover the major machinery controlling root growth in response to external stimuli. We found that cytokinin, one of the phytohormones, promotes transition from cell division to cell elongation, and that MYB3R transcription factor plays an important role in arresting cell division under DNA stress conditions.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (52 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results,  Acknowledgement Compliant: 6 results) Presentation (31 results) (of which Invited: 4 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Cyclin-dependent kinase-activating kinases CDKD;1 and CDKD;3 are essential for preserving mitotic activity in Arabidopsis thaliana2015

    • Author(s)
      Takatsuka, H., Umeda-Hara, C. and Umeda, M.
    • Journal Title

      Plant J.

      Volume: 印刷中

    • NAID

      120006537393

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Differential regulation of B2-type CDK accumulation in Arabidopsis roots2014

    • Author(s)
      Okushima, Y., Shimizu, K., Ishida, T., Sugimoto, K. and Umeda, M.
    • Journal Title

      Plant Cell Rep.

      Volume: 33 Issue: 7 Pages: 1033-1040

    • DOI

      10.1007/s00299-014-1581-z

    • NAID

      120006537389

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A dual-color marker system for in vivo visualization of cell cycle progression in Arabidopsis2014

    • Author(s)
      Yin, K., Ueda, M., Takagi, H., Kajihara, T., Sugamata Aki, S., Nobusawa, T., Umeda-Hara, M. Umeda
    • Journal Title

      Plant J.

      Volume: 80 Issue: 3 Pages: 541-552

    • DOI

      10.1111/tpj.12652

    • NAID

      120006537394

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Hormonal control of cell division and elongation along differentiation trajectories in roots2014

    • Author(s)
      Takatsuka, H. and Umeda, M.
    • Journal Title

      J. Exp. Bot.

      Volume: 65 Issue: 10 Pages: 2633-2643

    • DOI

      10.1093/jxb/ert485

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The role of SOG1, a plant-specific transcriptional regulator, in the DNA damage response2014

    • Author(s)
      Yoshiyama, K. O., Kimura, S., Maki, H., Britt, A. B. and Umeda, M.
    • Journal Title

      Plant Signal. Behav.

      Volume: 9 Issue: 4 Pages: 28889-28889

    • DOI

      10.4161/psb.28889

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cytokinins promote onset of endoreplication by controlling cell cycle machinery2014

    • Author(s)
      Takahashi, N. and Umeda, M.
    • Journal Title

      Plant Signal. Behav.

      Volume: 9 Issue: 8 Pages: e29396-e29396

    • DOI

      10.4161/psb.29396

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The Arabidopsis SIAMESE-RELATED Cyclin-Dependent Kinase Inhibitors SMR5 and SMR7 Regulate the DNA Damage Checkpoint in Response to Reactive Oxygen Species2014

    • Author(s)
      Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama Okamoto K, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L
    • Journal Title

      Plant Cell

      Volume: 26 Issue: 1 Pages: 296-309

    • DOI

      10.1105/tpc.113.118943

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytokinins control endocycle onset by promoting the expression of an APC/C activator in Arabidopsis roots2013

    • Author(s)
      Takahashi, N., Kajihara, T., Okamura, C., Kim, Y., Katagiri, Y., Okushima, Y., Matsunaga, S., Hwang, I. and Umeda, M.
    • Journal Title

      Curr. Biol.

      Volume: 23 Issue: 18 Pages: 1812-1817

    • DOI

      10.1016/j.cub.2013.07.051

    • NAID

      120005716839

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ATM-mediated phosphorylation of SOG1 is essential for the DNA damage response in Arabidopsis.2013

    • Author(s)
      Yoshiyama Okamoto K, Kobayashi J, Ogita N, Ueda M, Kimura S, Maki H, Umeda M
    • Journal Title

      EMBO Reports

      Volume: 14 Issue: 9 Pages: 817-822

    • DOI

      10.1038/embor.2013.112

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of very-long-chain fatty acids in the epidermis controls plant organ growth by restricting cell proliferation2013

    • Author(s)
      Nobusawa, T., Okushima, Y., Nagata, N., Kojima, M., Sakakibara, H. and Umeda, M.
    • Journal Title

      PLoS Biol.

      Volume: 11 Issue: 4 Pages: 1-14

    • DOI

      10.1371/journal.pbio.1001531

    • NAID

      120005716835

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Restriction of cell proliferation in internal tissues via the synthesis of very-long-chain fatty acids in the epidermis2013

    • Author(s)
      Nobusawa, T., Okushima, Y., Nagata, N., Kojima, M., Sakakibara, H. and Umeda, M.
    • Journal Title

      Plant Signal. Behav.

      Volume: 8 Issue: 8 Pages: 8-8

    • DOI

      10.4161/psb.25232

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kip-related protein 3 is required for control of endoreduplication in the shoot apical meristem and leaves of Arabidopsis2013

    • Author(s)
      Jun, S.E.
    • Journal Title

      Mol. Cells

      Volume: 35 Pages: 47-53

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Very-long-chain fatty acids have an essential role in plastid division by controlling Z-ring formation in Arabidopsis thaliana2012

    • Author(s)
      Nobusawa, T.
    • Journal Title

      Genes Cells

      Volume: 17 Pages: 709-719

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional repression of the APC/C activator CCS52A1 contributes to the active termination of cell growth2012

    • Author(s)
      Breuer, C.
    • Journal Title

      EMBO J.

      Volume: 31 Pages: 4488-4501

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis2011

    • Author(s)
      Adachi, S.
    • Journal Title

      Proc.Natl.Acad.Sci., USA

      Volume: 108 Issue: 24 Pages: 10004-10009

    • DOI

      10.1073/pnas.1103584108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell-cycle control and plant development2011

    • Author(s)
      Inagaki, S.
    • Journal Title

      Int.Rev.Cell Mol.Biol.

      Volume: 291 Pages: 227-261

    • DOI

      10.1016/b978-0-12-386035-4.00007-0

    • ISBN
      9780123860354
    • Related Report
      2011 Annual Research Report
  • [Journal Article] Two Arabidopsis cyclin A3s possess G1 cyclin-like features.2010

    • Author(s)
      Takahashi, I.
    • Journal Title

      Plant Cell Rep.

      Volume: 29 Pages: 307-315

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Brassinosteroids are involved in stem cell replenishment in Arabidopsis roots under DNA stress conditions2015

    • Author(s)
      藤本啓介、高橋直紀、梅田正明
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Regulation of auxin signaling is essential for stem cell maintenance under DNA stress conditions2015

    • Author(s)
      高橋直紀、梅田正明
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA損傷応答に関わる転写因子SOG1の標的遺伝子の解析2015

    • Author(s)
      荻田伸夫、奥島葉子、倉田哲也、時澤睦朋、山本義治、高橋直紀、梅田正明
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of genes involved in DNA damage response in Arabidopsis thaliana2015

    • Author(s)
      史佳卉、奥田利美、服部弘嗣、稲垣宗一、坂本智昭、倉田哲也、奥島葉子、梅田正明
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Stem cell replenishment maintains genome integrity2015

    • Author(s)
      梅田正明、藤本啓介、高橋直紀
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA damage response in Arabidopsis roots2014

    • Author(s)
      高橋直紀、Poyu Chen、梅田正明
    • Organizer
      Plant Genome Stability and Change
    • Place of Presentation
      アシロマ(アメリカ)
    • Year and Date
      2014-07-18 – 2014-07-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Cytokinins promote rapid cell elongation in Arabidopsis roots2014

    • Author(s)
      高塚大知、梅田正明
    • Organizer
      Auxins and Cytokinins in Plant Development International Symposium 2014
    • Place of Presentation
      プラハ(チェコ)
    • Year and Date
      2014-06-29 – 2014-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA damage response in Arabidopsis roots2014

    • Author(s)
      梅田正明
    • Organizer
      Plant Cell Cycle Workshop
    • Place of Presentation
      トジェボニ(チェコ)
    • Year and Date
      2014-06-29 – 2014-07-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Visualization of cell cycle progression using S/G2- and G2/M-specific reporters2014

    • Author(s)
      安喜(菅又)史織、高木瞳、Ke Yin、梅田(原)千景、植田美那子、梅田正明
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大、富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Actin rearrangement is essential for rapid cell elongation in Arabidopsis roots2014

    • Author(s)
      高塚大知、梅田正明
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大、富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Essential role of MYB3R transcription factors in genotoxic stress response2014

    • Author(s)
      陳柏佑、伊藤正樹、梅田正明
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大、富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cytokinin signaling is involved in DNA stress response in Arabidopsis roots2014

    • Author(s)
      高橋直紀、梅田正明
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大、富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] The essential function of cytokinin in promoting cell elongation in Arabidopsis roots2014

    • Author(s)
      梅田正明、高塚大知、高橋直紀
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大、富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cytokinins control endocycle onset by inducing an APC/C activator in Arabidopsis roots2013

    • Author(s)
      Takahashi, N.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functional role of cytokinin signaling in the transition zone of Arabidopsis roots2013

    • Author(s)
      Takatsuka, H.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functional analysis of RIR2R3-Myb proteins in genotoxic stress response2013

    • Author(s)
      Chen, P.-Y.
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cytokinin: A key hormone controlling cell division during organ growth2013

    • Author(s)
      Umeda, M.
    • Organizer
      UCDavis Plant Biology Graduate Group Seminar
    • Place of Presentation
      UC Davis
    • Year and Date
      2013-03-01
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] サイトカイニンシグナリングを介した根の細胞伸長における細胞骨格変動の解析2013

    • Author(s)
      高塚大知、梅田正明
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大、札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] ストレス時に細胞周期の停止状態を維持する分子機構の解析2013

    • Author(s)
      陳柏佑、伊藤正樹、梅田正明
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大、札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] Control of organ growth by restricting cell proliferation2013

    • Author(s)
      梅田正明
    • Organizer
      Annual Main Meeting of Society for Experimental Biology
    • Place of Presentation
      バレンシア会議場、バレンシア、スペイン
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cytokinins control two-step transition of root cells in Arabidopsis2013

    • Author(s)
      高塚大知、高橋直紀、梅田正明
    • Organizer
      Annual Main Meeting of Society for Experimental Biology
    • Place of Presentation
      バレンシア会議場、バレンシア、スペイン
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cytokinins control endocycle onset by promoting the expression of an APC/C activator in Arabidopsis roots2013

    • Author(s)
      高橋直紀、Y. Kim、I. Hwang、梅田正明
    • Organizer
      Annual Main Meeting of Society for Experimental Biology
    • Place of Presentation
      バレンシア会議場、バレンシア、スペイン
    • Related Report
      2013 Annual Research Report
  • [Presentation] Essential role of R1R2R3-Myb proteins in genotoxic stress response2013

    • Author(s)
      陳柏佑、伊藤正樹、梅田正明
    • Organizer
      Annual Main Meeting of Society for Experimental Biology
    • Place of Presentation
      バレンシア会議場、バレンシア、スペイン
    • Related Report
      2013 Annual Research Report
  • [Presentation] Cytokinin coordinates DNA ploidization and cell differentiation in roots2012

    • Author(s)
      Umeda, M.
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Cytokinins determine endocycle onset by controlling APC/CCS52A1 activity in Arabidopsis root2012

    • Author(s)
      Takahashi, N.
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cytokinins determine endocycle onset by modulating APC/CCS52A1 activity in Arabidopsis root2012

    • Author(s)
      高橋直紀
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都府)
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 表皮由来のシグナルによる器官成長の制御2012

    • Author(s)
      梅田正明
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都府)(シンポジウム講演)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Very-long-chain fatty acids synthesized in the epidermis control plant organ growth2011

    • Author(s)
      梅田正明
    • Organizer
      The 3^<rd> Korea-Japan Young Plant Scientist Symposium "Plant Biomass Biology
    • Place of Presentation
      Pohang University of Science and Technology(韓国)(招待講演)
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] イメージングと顕微操作で迫る細胞分裂と組織形成の統御機構2011

    • Author(s)
      植田美那子
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス(東京都)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of B-type CDK accumulation during Arabidopsis root development.2010

    • Author(s)
      奥島葉子
    • Organizer
      第33回日本分子生物学会年会、第83回日本生化学会大会 合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA二本鎖切断による核内倍加の誘導機構2010

    • Author(s)
      梅田正明
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      愛知県春日井市
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Book] Cell Biology2014

    • Author(s)
      Takahashi, N. and Umeda, M.
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Remarks] 植物成長制御研究室ホームページ

    • URL

      http://bsw3.naist.jp/umeda/index.html

    • Related Report
      2014 Annual Research Report 2012 Annual Research Report
  • [Remarks] 研究内容

    • URL

      http://bsw3.naist.jp/umeda/project.html

    • Related Report
      2011 Annual Research Report
  • [Remarks] 研究成果

    • URL

      http://bsw3.naist.jp/umeda/achievement.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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