| Budget Amount *help |
¥83,070,000 (Direct Cost: ¥63,900,000、Indirect Cost: ¥19,170,000)
Fiscal Year 2014: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2012: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2011: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2010: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
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| Outline of Final Research Achievements |
In this project, we aimed to elucidate molecular mechanisms of cold response in plants to develop cold tolerance, from cold perception to cellular responses. The results revealed that 1) cold acclimation was affected by not only temperature but also light quality, 2) a newly developed in planta monitoring system of gene expression (using a transgenic plant containing a construct of the promoter of the interested gene combined with luciferase gene) was powerful to analyze temporal and special expression patterns of cold-regulated gene during cold acclimation, 3) behavior of ER and Ca in response to cold or freezing could be monitored using unique system of laser scanning confocal microscope and temperature-controlled sample stage, and 4) a cold-induced GPI-anchored protein associated with the plasma membrane was functionally associated with the increase of freezing tolerance. With these results, we discussed the mechanism of temperature perception and signal transduction in plants.
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