| Budget Amount *help |
¥104,390,000 (Direct Cost: ¥80,300,000、Indirect Cost: ¥24,090,000)
Fiscal Year 2014: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2012: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Fiscal Year 2011: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2010: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
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| Outline of Final Research Achievements |
Light is one of the important environmental signals for plant. Plant has multiple blue light receptors e.g. phototropin, cryptochrome, etc. and a red light receptor, phytochrome, to perceive light signals. We have studied the structures and functions of these photoreceptors and obtained the following results. 1) Phototropin is a blue light-regulated Ser/Thr kinase. Using Arabidopsis and Chlamydomonas phototropin with various constructs, we performed structural and amino acid substitution studies and got useful information concerning the molecular mechanism of light regulation of the kinase. 2) Phytochrome is a red / far-red light reversible photoreceptor. We established the preparation system for the photoactive N-terminal Sensory Module and its variants of Arabidopsis phytochrome B. Useful information concerning their molecular structures and new information regarding the interaction between the phytochrome B and PIF3, a signal transduction component, were obtained.
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