| Budget Amount *help |
¥72,540,000 (Direct Cost: ¥55,800,000、Indirect Cost: ¥16,740,000)
Fiscal Year 2014: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2013: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2010: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
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| Outline of Final Research Achievements |
Cell and tissue-specific gene expression profiles are important for understanding how multicellular organisms function and develop. We report a novel, highly reliable position-specific gene expression profiling method developed for plants. It consists of an automatically dissecting system with a narrow, sharpened needle coupled with an optimized protocol to synthesize a high-quality cDNA library on magnetic beads using a single micro-sample. Using this method, gene expression patterning and clustering at successive 50-μm intervals was demonstrated in the shoot apex of a 4-day-old Arabidopsis seedling.
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