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Development and application of high resolution imaging mass spectrocope

Planned Research

Project AreaEnvironmental sensing of plants: Signal perception, processing and cellular responses
Project/Area Number 22120009
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

TAKAHASHI Katsutoshi  独立行政法人産業技術総合研究所, 計測フロンティア研究部門, 主任研究員 (00271792)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥76,310,000 (Direct Cost: ¥58,700,000、Indirect Cost: ¥17,610,000)
Fiscal Year 2014: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2012: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2011: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2010: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Keywords質量顕微鏡 / フーリエ変換質量分析 / 植物組織 / 代謝物 / 空間分布 / FTMS / 植物生理学 / MALDI / Imaging MS / MALDI-MS / 組織切片 / 紫外パルスレーザー
Outline of Final Research Achievements

Imaging mass spectroscopy technique was used to map spacial distribution of metabolites, sugars, lipids and proteins on a thin slice of animal tissues, but it was very difficult to apply to the plant tissue analysis. In this study, the hardware and software was implemented to enable to map spacial distribution of metabolites, sugars and lipids on a intact plant tissue, not only on a thin sliced tissue, The special software to pick up the spacial distributions of the ions from several hundreds giga bytes of the data produced by the imaging mass spectroscope was also successfully developed.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (21 results) (of which Invited: 8 results) Remarks (3 results)

  • [Journal Article] Power of Isotopic Fine Structure for Unambiguous Determination of Metabolite Elemental Compositions: in silico Evaluation and Metabolomic Application2014

    • Author(s)
      Nagao T., Yukihira D., Fujimura Y., Saito K., Takahashi K., Miura D. and Wariishi H.
    • Journal Title

      Analytica Chimica Acta

      Volume: 813 Pages: 70-76

    • DOI

      10.1016/j.aca.2014.01.032

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Infrared Multiple Photon Dissociation Spectroscopy and Computational Studies of O-Glycosylated Pentides2012

    • Author(s)
      福井一彦、高橋勝利
    • Journal Title

      Analytical Chemistry

      Volume: 84 Issue: 5 Pages: 2188-2194

    • DOI

      10.1021/ac202379v

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Strategy for the Determination of the Elemental Composition by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Based on Isotopic Peak Ratios2011

    • Author(s)
      Miura D., Tsuji Y., Takahashi K., Wariishi H, Saito K.
    • Journal Title

      ANALYTICAL CHEMISTRY

      Volume: 82 Pages: 5887-5891

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MALDI-MS-Based High-Throughput Metabolite Analysis for Intracellular Metabolic Dynamics.2011

    • Author(s)
      Yukihira D., Miura D., Saito K., Takahashi K, Wariishi H.
    • Journal Title

      ANALYTICAL CHEMISTRY

      Volume: 82 Pages: 4278-4282

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] High Resolution MALDI/LDI FTICR Mass Spectrometry Imaging of Plant Tissues2015

    • Author(s)
      K.Takahashi, A.Anegawa and T.Kozuka
    • Organizer
      The 2nd International Symposium on Plant Environmental Sensing
    • Place of Presentation
      AIST water front, Tokyo, Japan
    • Year and Date
      2015-03-13 – 2015-03-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MALDI/LDI-FTICR Mass Spectrometry Imaging for Plant Tissue Analysis to Distinguish Changes in Metabolite Distributions under Different Stimulus Environments2014

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      Ourcon II
    • Place of Presentation
      Papillon Ayscha Hotel, Antalya, Turcky
    • Year and Date
      2014-11-18 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] LabMSI: a software Tool to Analyze High Resolution Mass Spectrometry Imaging Data2014

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      Ourcon II
    • Place of Presentation
      Papillon Anscha Hotel, Antalya, Turcky
    • Year and Date
      2014-11-18 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 質量顕微鏡による植物組織内代謝物のマッピング2014

    • Author(s)
      高橋勝利
    • Organizer
      シンポジウム 植物の繁殖戦略を考える
    • Place of Presentation
      名古屋
    • Year and Date
      2014-11-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MALDI/LDI-FTICR Mass Spectrometry Imaging for Plant Tissue Analysis to Distinguish Cahnges in Metabolite Distributions under Different Stimulus Environments2014

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      ASMS 2014
    • Place of Presentation
      The Baltimore Convention Center, Baltimore, MD, USA
    • Year and Date
      2014-06-15 – 2014-06-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] MALDI/LDI-FTMS imaging of intact plant tissues2014

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      Desorption 2014
    • Place of Presentation
      Hilton Vonaventure, Montorial, Canada
    • Year and Date
      2014-04-14 – 2014-04-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] LabMSI: a software Tool to Analyze High Resolution Mass Spectrometry Imaging Data2014

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      Desorption 2014
    • Place of Presentation
      Hilton Vonaventure, Montrial, Canada
    • Year and Date
      2014-04-14 – 2014-04-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] High resolution MALDI/LDI-FTICR mass spectrometry imaging of plant tissues2013

    • Author(s)
      Takahashi K.
    • Organizer
      Innovation in Mass Spectrometry Instrumentation Conference 2013
    • Place of Presentation
      Saint-Petersburg, Russia
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Direct Mass Spectrometry Imaging of Intact Tissues of Arabidopsis2013

    • Author(s)
      Takahashi K.
    • Organizer
      International Conference on Arabidopsis Research 2013
    • Place of Presentation
      シドニー(オーストラリア)
    • Related Report
      2013 Annual Research Report
  • [Presentation] LabMSI: a software too to analyze High resolution mass spectrometry imaging2013

    • Author(s)
      Takahashi K.
    • Organizer
      Innovation in Mass Spectrometry Instrumentation Conference 2013
    • Place of Presentation
      Saing-Petersburg, Russia
    • Related Report
      2013 Annual Research Report
  • [Presentation] 質量顕微鏡で植物組織を見る2012

    • Author(s)
      高橋勝利
    • Organizer
      植物環境感覚 第3回若手の会
    • Place of Presentation
      静岡県浜松市
    • Year and Date
      2012-10-16
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] FT-MSを用いた植物代謝物のイメージング2012

    • Author(s)
      高橋勝利
    • Organizer
      第7回メタボロームシンポジウム
    • Place of Presentation
      山形県鶴岡市
    • Year and Date
      2012-10-12
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Terpenoid indole alkaloids synthesis in Catharanthus roseus2012

    • Author(s)
      Kotaro Yamamoto
    • Organizer
      New Frondiers of Metabolism Research in Biomedical Sciences
    • Place of Presentation
      Tokyo
    • Year and Date
      2012-09-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] AISTimzMLviewer developed for viewing Hi-res and big FTICR MSI data2012

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      1st imzML workshop
    • Place of Presentation
      Rauischholzhausen, Germany
    • Year and Date
      2012-09-25
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 植物組織の質量イメージング分析2012

    • Author(s)
      高橋勝利
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      石川県金沢市
    • Year and Date
      2012-09-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Imaging Mass Spectrometry of Plant Tissue Slices: Comparison of MALDI-TOF MSI and MALDI-FTICR MSI2012

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      IMSC2013
    • Place of Presentation
      Kyoto
    • Year and Date
      2012-09-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] MALDI-FTMS Imaging: a powerful tool for imaging plant tissue slices2012

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      OurCon2012
    • Place of Presentation
      Ourense, Spain
    • Year and Date
      2012-09-03
    • Related Report
      2012 Annual Research Report
  • [Presentation] Imaging Mass Spectrometry of Arabidopsis Leaf Slices2012

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      ICAR2012
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2012-07-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] Imaging Mass Spectrometry of Plant Tissue Slices using FTICR-MS2012

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      Desorption2012
    • Place of Presentation
      Marburg, Germany
    • Year and Date
      2012-06-05
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Imaging Mass Spectrometry Technique for Identifying and Locationg Unknown Molecular Species in Tissue Slices2012

    • Author(s)
      高橋勝利
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都府・京都産業大学(招待講演)
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Imaging Mass Spectrometry Tchnique for Identifying and Locating Unknown Molecular Species in Tissue Slices.2011

    • Author(s)
      Katsutoshi Takahashi
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Remarks] 植物の環境感覚:刺激受容から細胞応答まで

    • URL

      http://esplant.net/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://esplant.net/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://esplant.net/index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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