| Budget Amount *help |
¥67,340,000 (Direct Cost: ¥51,800,000、Indirect Cost: ¥15,540,000)
Fiscal Year 2014: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2013: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2012: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2011: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2010: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
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| Outline of Final Research Achievements |
When an intense femtosecond laser is focused on biological materials under microscope, they are dissected at the laser focal point because of the effective multiphoton absorption. Furthermore, when the laser is focused into aqueous medium, an explosion is induced at the laser focal point and shock- and stress- waves propagate and act to the surrounding material as an impulsive force. In this research program, we developed new methodology to investigate plant physiology adapting these phenomena to plants. Physiological function of plant was inhibited by the laser processing of targeted cells inside plant body. Adhesion between intercellular suborganelles were broken by the impulsive force and the adhesion strength was quantified convening with technique of atomic force microscopy. Furthermore, we developed new methods to investigate freezing tolerance of plant body utilizing laser-induced ice formation phenomenon and to introduce exogenous molecules to single plant cells.
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