| Budget Amount *help |
¥97,240,000 (Direct Cost: ¥74,800,000、Indirect Cost: ¥22,440,000)
Fiscal Year 2014: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2013: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2011: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2010: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
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| Outline of Final Research Achievements |
Protein complexes are essential for various kinds of physiological functions in the human body. By developing single particle analysis (SPA) using Transmission electron microscopy (TEM), the three-dimensional (3D) structure of signal processing protein complexes. Further, these structures including SecDF are interpreted by docking partial structure determined by X-ray crystallography.
The new atmospheric scanning electron microscope (ASEM) observes samples through electron-transparent film. Therefore, samples can be set in solution under an open atmosphere. Using this system, immuno-ASEM was successfully developed, and we have observed intracellular super-molecular-complex formations, including accumulation of STIM-1 in response to Ca2+ store depletion. Moreover, the observation method of small protein crystals are developed, and contributed to X-ray crystallography. Further, by multi-windowning ASEM, the observation efficiency was improved.
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