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Electron microscopy of structure and localization of signal processing complexes

Planned Research

Project AreaStructural basis of cell-signalling complexes mediating signal perception, transduction and responses
Project/Area Number 22121004
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

SATO Chikara  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (00357146)

Co-Investigator(Renkei-kenkyūsha) KAWATA Masaaki  独立行政法人産業技術総合研究所, 人工知能研究センター, 主任研究員 (20356843)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥97,240,000 (Direct Cost: ¥74,800,000、Indirect Cost: ¥22,440,000)
Fiscal Year 2014: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2013: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2011: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2010: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Keywordsシグナル伝達 / 分子複合体 / 電子顕微鏡 / タンパク質構造 / イオンチャンネル / イオンチャネル
Outline of Final Research Achievements

Protein complexes are essential for various kinds of physiological functions in the human body. By developing single particle analysis (SPA) using Transmission electron microscopy (TEM), the three-dimensional (3D) structure of signal processing protein complexes. Further, these structures including SecDF are interpreted by docking partial structure determined by X-ray crystallography.
The new atmospheric scanning electron microscope (ASEM) observes samples through electron-transparent film. Therefore, samples can be set in solution under an open atmosphere. Using this system, immuno-ASEM was successfully developed, and we have observed intracellular super-molecular-complex formations, including accumulation of STIM-1 in response to Ca2+ store depletion. Moreover, the observation method of small protein crystals are developed, and contributed to X-ray crystallography. Further, by multi-windowning ASEM, the observation efficiency was improved.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (93 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (36 results) (of which Peer Reviewed: 36 results,  Open Access: 2 results,  Acknowledgement Compliant: 8 results) Presentation (55 results) (of which Invited: 11 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Observation of tissues in open aqueous solution by atmospheric scanning electron microscopy: applicability to intraoperative cancer diagnosis2015

    • Author(s)
      N.Memtily, T.Okada, T.Ebihara, M.Sato, A.Kurabayashi, M.Furihata, M.Suga, H.Nishiyama, K.Mio, C.Sato
    • Journal Title

      Int J Oncol

      Volume: 46 Issue: 5 Pages: 1872-1882

    • DOI

      10.3892/ijo.2015.2905

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crystal structure of the Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex2015

    • Author(s)
      T.Numata, H.Inanaga, C.Sato, and T.Osawa
    • Journal Title

      J Mol Biol

      Volume: 427 Issue: 2 Pages: 259-273

    • DOI

      10.1016/j.jmb.2014.09.029

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New simulated annealing approach considering helix bending applied to determine the 8.8 A structure of 15-protofilament microtubules2014

    • Author(s)
      T.Ogura, H. Yajima, R.Nitta, N.Hirokawa, C.Sato
    • Journal Title

      J Struct Biol

      Volume: 188 Issue: 2 Pages: 165-176

    • DOI

      10.1016/j.jsb.2014.08.009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immuno-Electron Microscopy of Primary Cell Cultures from GeneticallyModified Animals in Liquid by Atmospheric Scanning Electron Microscopy (ASEM)2014

    • Author(s)
      T.Kinoshita, Y.Mori, K.Hirano, S.Sugimoto, K.Okuda, S.Matsumoto, T.Namiki, T.Ebihara, M.Kawata, H.Nishiyama, M.Sato, M.Suga, K.Higashiyama, K.Sonomoto, Y.Mizunoe, S.Nishihara, C.Sato
    • Journal Title

      Microscopy and Microanalysis

      Volume: 20 Issue: 2 Pages: 469-483

    • DOI

      10.1017/s1431927614000178

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Electron microscopy of primary cell cultures in solution and correlative optical microscopy using ASEM2014

    • Author(s)
      K.Hirano, T.Kinoshita, T.Uemura, H.Motohashi, Y.Watanabe, T.Ebihara, H.Nishiyama, M.Sato, M.Suga, Y.Maruyama, N.M.Tsuji, M.amamoto, S.Nishihara, C.Sato
    • Journal Title

      Ultramicroscopy

      Volume: 143 Pages: 52-66

    • DOI

      10.1016/j.ultramic.2013.10.010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Conformational variation of the translocon enhancing chaperone SecDF2014

    • Author(s)
      K.Mio, T.Tsukazaki, H.Mori, M.Kawata, T.Moriya, Y.Sasaki, R.Ishitani, K.Ito, O.Nurekiand C.Sato
    • Journal Title

      J Struct Funct Genomics

      Volume: 15 Issue: 3 Pages: 107-115

    • DOI

      10.1007/s10969-013-9168-4

    • NAID

      120005716853

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Small effect of upcoming reward outcomes on visual cue-related neuronal activity in macaque area TE during conditional association2014

    • Author(s)
      K.Ohyama, Y.Sugase-Miyamoto, N.Matsumoto, C.Sato, M.Shidara
    • Journal Title

      Neuroscience Research

      Volume: 88 Pages: 28-38

    • DOI

      10.1016/j.neures.2014.08.004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Atmospheric Scanning Electron Microscope System with an Open Sample Chamber: Configuration and Applications2014

    • Author(s)
      H.Nishiyama, M.Koizumi, K.Ogawa, S.Kitamura, Y.Konyuba, Y.Watanabe, N.Ohbayashi, M.Fukuda, M.Suga and C.Sato
    • Journal Title

      Ultramicroscopy

      Volume: 147 Pages: 86-97

    • DOI

      10.1016/j.ultramic.2014.06.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 水の中の世界を電子顕微鏡で観る2014

    • Author(s)
      佐藤主税
    • Journal Title

      顕微鏡

      Volume: 49 Pages: 2-2

    • NAID

      130007701782

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 大気に開放された試料室を持つ大気圧走査電子顕微鏡の開発2014

    • Author(s)
      西山英利,小泉充,小川康司,北村真一,小入羽祐治,須賀三雄,佐藤主税
    • Journal Title

      顕微鏡

      Volume: 49 Pages: 3-6

    • NAID

      130007701690

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 巨核球による血小板産生と樹状細胞による細菌貪食の液中電顕観察2014

    • Author(s)
      佐藤主税,渡邊要平,丸山雄介,佐藤真理,山本雅之,辻典子,本橋ほづみ
    • Journal Title

      顕微鏡

      Volume: 49 Pages: 14-17

    • NAID

      130007701784

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 単粒子構造解析法と水中観察電顕ASEMによる膜タンパク質複合体の研究2014

    • Author(s)
      佐藤主税
    • Journal Title

      ファルマシア

      Volume: 50 Pages: 423-427

    • NAID

      130005158663

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TRPチャネルとOrai-STIM複合体系の構造と局在の解析2014

    • Author(s)
      三尾和弘, 丸山雄介, 小椋俊彦, 佐藤主税
    • Journal Title

      実験医学

      Volume: 32 Pages: 540-548

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 大気圧電子顕微鏡(ASEM)による抗体を選ばない水中免疫電顕法:細胞・タンパク質ミクロ結晶の液中観察2014

    • Author(s)
      海老原達彦, 西山英利, 佐藤真理, 千田俊哉, 須賀三雄, 佐藤主税
    • Journal Title

      生化学

      Volume: 86 Pages: 103-110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Positively Charged Nanogold Label Allows the Observation of Fine Cell Filopodia and Flagella in Solution by Atmospheric Scanning Electron Microscopy2014

    • Author(s)
      H.Nishiyama, K.Teramoto, M.Suga, and C.Sato.
    • Journal Title

      Microscopy Research and Technique

      Volume: 77 Issue: 2 Pages: 153-160

    • DOI

      10.1002/jemt.22322

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 正帯電金ナノ粒子標識法による鞭毛・線毛・細胞突起の大気圧走査電子顕微鏡観察2013

    • Author(s)
      西山英利、寺本華奈江、須賀三雄、佐藤主税
    • Journal Title

      顕微鏡

      Volume: 48 Pages: 190-194

    • NAID

      130007705268

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 大気圧走査電子顕微鏡(ASEM)による水中免疫電顕法:タンパク質複合体のダイナミックな離合集散と細胞内移動2013

    • Author(s)
      海老原達彦、村井稔幸、西山英利、佐藤真理、須賀三雄、佐藤主税
    • Journal Title

      顕微鏡

      Volume: 48 Pages: 107-112

    • NAID

      10031194755

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electron-light Correlative Microscopy in Open Solution by Atmospheric Scanning Electron Microscope (ASEM)2013

    • Author(s)
      T.Ebihara, H.Nishiyama, M.Suga, C.Sato
    • Journal Title

      Advances in Imaging and Electron Physics

      Volume: 179 Pages: 152-154

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression, purification, crystallization and preliminary crystallographic analysis of hepatitis B virus core protein dimerized via a peptide linker containing an EGFP insertion2013

    • Author(s)
      M.Kikuchi, S.Iwabuchi, T.Kikkou, K.Noguchi, M.Odaka, M.Yohda, M.Kawata, C.Sato, O.Matsumoto
    • Journal Title

      Acta Cryst

      Volume: F69 Issue: 8 Pages: 942-945

    • DOI

      10.1107/s1744309113019957

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 簡単・迅速前処理の大気圧走査電子顕微鏡JASM-62002013

    • Author(s)
      須賀三雄、西山英利、寺本華奈江、渡部善幸、佐藤主税
    • Journal Title

      生物試料分析

      Volume: 36 Pages: 235-244

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 大気圧電子顕微鏡(ASEM)による抗体を選ばない水中免疫電顕法 CRACチャネルの引き金となる、ER内Ca2+枯渇によるSTIM1凝集の可視化2013

    • Author(s)
      佐藤主税、西山英利、須賀三雄、佐藤真理、海老原達彦
    • Journal Title

      日薬理誌

      Volume: 141 Pages: 245-250

    • NAID

      10031171125

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel Convergence-Oriented Approach for Evaluation and Optimization of Workflow in Single Particle 2D Averaging of Electron Microscope Images,2013

    • Author(s)
      T.Moriya, K.Mio, C.Sato
    • Journal Title

      Microscopy

      Volume: 62 Issue: 5 Pages: 491-513

    • DOI

      10.1093/jmicro/dft026

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multi-reference-based multiple alignment statistics enables accurate protein particle pickup from noisy images2013

    • Author(s)
      M.Kawata, C.Sato
    • Journal Title

      Microscopy

      Volume: 62 Issue: 2 Pages: 303-315

    • DOI

      10.1093/jmicro/dfs075

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Susceptibility test of two ca-atpase conformers to denaturants and polyols to outline their structural difference.2013

    • Author(s)
      A.Kotake
    • Journal Title

      J.Membr.Biol.

      Volume: 246 Issue: 2 Pages: 141-149

    • DOI

      10.1007/s00232-012-9513-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression, purification, crystallization and preliminary X-ray crystallographic studies of hepatitis B virus core fusion protein corresponding to octahedral particles.2013

    • Author(s)
      M.Kikuchi
    • Journal Title

      Acta Cryst.

      Volume: F69 Issue: 2 Pages: 165-169

    • DOI

      10.1107/s1744309112052074

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conformational changes in tubulin in GMPCPP and GDP-taxol microtubules observed by cryoelectron microscopy.2012

    • Author(s)
      Yajima, H., T. Ogura, R. Nitta, Y. Okada, C. Sato, and N. Hirokawa.
    • Journal Title

      Journal of Cell Biology

      Volume: 198 Issue: 3 Pages: 315-322

    • DOI

      10.1083/jcb.201201161

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct Observation of Protein Microcrystals in crystallization buffer by Atmospheric Scanning Electron Microscopy.2012

    • Author(s)
      Y.Maruyama
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 13 Issue: 8 Pages: 10553-10567

    • DOI

      10.3390/ijms130810553

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immuno EM-OM Correlative Microscopy in Solution by Atmospheric Scanning Electron Microscopy (ASEM).2012

    • Author(s)
      Y.Maruyama
    • Journal Title

      J. Struct. Biol.

      Volume: 180 Issue: 2 Pages: 259-270

    • DOI

      10.1016/j.jsb.2012.08.006

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stimulus-Related Activity during Conditional Associations in Monkey Perirhinal Cortex Neurons Depends on Upcoming Reward Outcome2012

    • Author(s)
      Kaoru Ohyama
    • Journal Title

      The Journal of Neuroscience

      Volume: 32 Issue: 48 Pages: 17407-17419

    • DOI

      10.1523/jneurosci.2878-12.2012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid imaging of mycoplasma in solution using Atmospheric Scanning Electron Microscopy (ASEM)2012

    • Author(s)
      Sato C, Manaka S, Nakane D, Nishiyama H, Suga M, Nishizaka T, Miyata M, Maruyama Y
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 417 Issue: 4 Pages: 1213-1218

    • DOI

      10.1016/j.bbrc.2011.12.111

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitsugumin 23 forms a massive bowl shaped assembly and cation-conducting channel2011

    • Author(s)
      Elisa Venruti, Kazuhiro Mio, Miyuki Nishi, Toshihiko Ogura, Toshio Moriya, Samantha Pitt, Kazutaka Okuda, Sho Kakizawa, Rebecca Sitsapesan, Chikara Sato and Hiroshi Takeshima
    • Journal Title

      Biochemistry

      Volume: Vol.50 Issue: 13 Pages: 2623-2632

    • DOI

      10.1021/bi1019447

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional structure of the signal peptide peptidase2011

    • Author(s)
      H.Miyashita
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Issue: 29 Pages: 26188-26197

    • DOI

      10.1074/jbc.m111.260273

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Atmospheric Scanning Electron Microscope with open sample space observes dynamic phenomena in liquid or gas2011

    • Author(s)
      M.Suga
    • Journal Title

      Ultramicroscopy

      Volume: 111 Issue: 12 Pages: 1650-1658

    • DOI

      10.1016/j.ultramic.2011.08.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 大気圧走査電子顕微鏡ASEMによる液体・気体中での動的観察2011

    • Author(s)
      須賀三雄
    • Journal Title

      顕微鏡

      Volume: 46 Pages: 137-139

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The C-terminal coiled-coil of the bacterial voltage-gated sodium channel NaChBac is not essential for tetramer formation, but stabilizes subunit-to-subunit interactions2010

    • Author(s)
      K.Mio
    • Journal Title

      PROG BIOPHYS MOL BIO

      Volume: VOL.103 Pages: 111-121

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single particle reconstruction of membrane proteins : A tool for understanding the 3D structure of disease-related macromolecules2010

    • Author(s)
      K.Mio
    • Journal Title

      PROG BIOPHYS MOL BIO

      Volume: VOL.103 Pages: 122-130

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Observation of Cells and Tissues immersed in aqueous solution using Atmospheric Scanning Electron Microscopy (ASEM)2015

    • Author(s)
      C.Sato
    • Organizer
      IGER International Symposium on Frontiers in Biological Research with Advanced Electron Microscope Technologies
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2015-01-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Electron microscopy of proteins and cells in water and ice2014

    • Author(s)
      C.Sato
    • Organizer
      2th International Conference on X-Ray Microscopy 2014
    • Place of Presentation
      Melbourne (オーストラリア)
    • Year and Date
      2014-10-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Atmospheric scanning electron microscopy (ASEM) of axonal segmentation in neurons and phagocytosis of dendritic cells2014

    • Author(s)
      C. Sato
    • Organizer
      19th World Congress on Advances in Oncology and 17th International Symposium on Molecular Medicine
    • Place of Presentation
      Athens(ギリシャ)
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Fine structures of biofilms in solution visualized by atmospheric scanning electron microscopy2014

    • Author(s)
      S. Sugimoto, K.-I. Okuda, M. Sato, C. Sato, Y. Mizunoe
    • Organizer
      9th World Congress on Advances in Oncology and 17th International Symposium on Molecular Medicine
    • Place of Presentation
      Athens(ギリシャ)
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] TEM単粒子解析と大気圧電子顕微鏡(ASEM)によるタンパク質複合体形成の観察2014

    • Author(s)
      佐藤主税,三尾和弘,マミテリナシルハジ,佐藤真理,海老原達彦,小椋俊彦
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大気圧SEM (Atmospheric SEM: ASEM)2014

    • Author(s)
      佐藤主税
    • Organizer
      SCAN TECH 2014
    • Place of Presentation
      東京都市大学(東京都世田谷区)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] he Atmospheric Scanning Electron Microscope (ASEM) Observes Axonal Segmentation and Platelet Generation in Solution2014

    • Author(s)
      T. Kinoshita, H. Motohashi, K. Hirano, Y. Maruyama, M. Kawata, T. Ebihara, M. Sato, H. Nishiyama, M. Suga, M. Yamamoto, S Nishihara and C. Sato.
    • Organizer
      IMC2014
    • Place of Presentation
      Praque (チェコ)
    • Year and Date
      2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Observation of Tissues in Solution by Atmospheric Scanning Electron Microscope (ASEM)2014

    • Author(s)
      N.Memtily, K.Mio, M.Sato, T.Ebihara, C.Sato
    • Organizer
      M&M2014
    • Place of Presentation
      Hartford(USA)
    • Year and Date
      2014-08-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] The Atmospheric Scanning Electron Microscope (ASEM) Observes Axonal Segmentation and Synaptic Induction in Solution2014

    • Author(s)
      C.Sato, M.Sato T.Kinoshita, K.Hiarno, S.Nishihara, T.Uemura, H.Nishiyama, M.Suga.
    • Organizer
      M&M2014
    • Place of Presentation
      Hartford(USA)
    • Year and Date
      2014-08-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 水や氷中の細胞・複合体をそのまま観ることができる電子顕微鏡2014

    • Author(s)
      佐藤主税
    • Organizer
      第4回理研GSC 七夕ミーティング
    • Place of Presentation
      理化学研究所横浜キャンパス(横浜市)
    • Year and Date
      2014-07-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 大気圧走査電子顕微鏡(ASEM) による培養神経細胞の観察2014

    • Author(s)
      海老原 達彦,Memtily Nassirhadiy, 西山英利,佐藤 真理,須賀三雄,佐藤 主税
    • Organizer
      第28回モロシヌス研究会
    • Place of Presentation
      修善寺総合会館(伊豆市)
    • Year and Date
      2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 受容体でコートした磁気ビーズによる初代培養シナプス形成誘導のASEM水中免疫電顕2014

    • Author(s)
      植村健,佐藤 真理,西山英利,須賀三雄,佐藤 主税
    • Organizer
      日本顕微鏡学会第70回記念学術講演会
    • Place of Presentation
      幕張メッセ国際会議(千葉市)
    • Year and Date
      2014-05-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Immuno EM-OM Correlative Microscopy in Solution by Atmospheric Scanning Electron Microscopy (ASEM) and Its Application to Protein Microcrystals and Mycoplasma2012

    • Author(s)
      佐藤主税
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] Electron-light correlative microscopy in open solution by Atmospheric Scanning Electron Microscope (ASEM)2012

    • Author(s)
      佐藤主税
    • Organizer
      CONFERENCE ON IN-SITU AND CORRELATIVE ELECTRON MICROSCOPY (CISCEM)
    • Place of Presentation
      Saarbrucken, Germany
    • Year and Date
      2012-11-06
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 大気圧走査電子顕微鏡(ASEM)による水中免疫電顕法とマイコプラズマの観察2012

    • Author(s)
      佐藤主税
    • Organizer
      第95回 日本細菌学会 関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      2012-10-11
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大気圧走査電子顕微鏡を用いた様々な応用例2012

    • Author(s)
      佐藤主税
    • Organizer
      第44回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      高知
    • Year and Date
      2012-09-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] Direct electron microscopy of protein crystals and Mycoplasma cells in solution using the Atmospheric SEM.2012

    • Author(s)
      佐藤主税
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] TEM image-based structure analysis of ion channels, and their subcellular localization revealed by newly developed ASEM.2012

    • Author(s)
      佐藤主税
    • Organizer
      COLD SPRING HARBOR ASIA CONFERENCES
    • Place of Presentation
      蘇州
    • Year and Date
      2012-09-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] In-solution EM Observation of Cells and Tissues using the Atmospheric Scanning Electron Microscope (ASEM): High Throughtput Immunocytochemistry and Intra Operative Cancer Diagnosis with Wet Tissues.2012

    • Author(s)
      佐藤主税
    • Organizer
      14th international Congress of Histochemistry and Cytochemistry
    • Place of Presentation
      京都
    • Year and Date
      2012-08-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大気圧走査電子顕微鏡によるマイコプラズマの水中での迅速観察法の開発 Rapid imaging of mycoplasma in solution using Armospheric SEM(ASEM) .2012

    • Author(s)
      佐藤主税
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Place of Presentation
      つくば
    • Year and Date
      2012-05-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大気圧電子顕微鏡ASEMによる水中免疫電顕法:神経細胞などでのタンパク質局在の迅速決定2011

    • Author(s)
      佐藤主税
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Atmospheric scanning electron microscopy (asem) realizes direct em-om correlative immuno-cytochemistry of neurons in solution2011

    • Author(s)
      佐藤主税
    • Organizer
      Neuroscience 2011
    • Place of Presentation
      Washington Convention Center (Washington, DC, USA)
    • Year and Date
      2011-11-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Direct electron microscopy of Mycoplasma in solution using the Atmospheric SEM2011

    • Author(s)
      佐藤主税
    • Organizer
      第5回アジアマイコプラズマ学会・第38回日本マイコプラズマ学会学術集会
    • Place of Presentation
      長崎県医師会館(長崎県)(招待講演)
    • Year and Date
      2011-10-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Direct ASEM immuno-electron microscopy of eells in solution2011

    • Author(s)
      佐藤主税
    • Organizer
      16th World Congress on Advances in Oncology and 14th Symposium on Molecular Medicine
    • Place of Presentation
      Hotel Rodos Palace, (Rhodes Island, Greece)(招待講演)
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] 3D structure of intramembrane-cleaving proteases and oxidative stress sensors using electron microscopy2011

    • Author(s)
      三尾和弘
    • Organizer
      16th World Congress on Advances in Oncology and 14th Symposium on Molecular Medicine
    • Place of Presentation
      Hotel Rodos Palace, (Rhodes Island, Greece)(招待講演)
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Protein dynamism revealed by single particle reconstruction and protein localization observed by atmospheric SEM (ASEM)2011

    • Author(s)
      佐藤主税
    • Organizer
      第49回生物物理学会年会
    • Place of Presentation
      兵庫県立大学姫路書与キャンパス(兵庫県)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Immuno-EM of fine growth cone and synapse structures in aqueous solution using the atmospheric scanning electron microscope2011

    • Author(s)
      佐藤主税
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大気圧電顕による水中免疫ラベルと透過電顕単粒子解析によるタンパク質ダイナミズムの解析2011

    • Author(s)
      佐藤主税
    • Organizer
      生理学研究所研究会「作動中の膜機能分子の姿を捉える-静止画から動画へ-」
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県)
    • Year and Date
      2011-09-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Atmospheric SEM observes samples in liquid2011

    • Author(s)
      須賀三雄
    • Organizer
      JAIMA Discussion on Analytical Science and Technology 2011
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Immuno-EM in Buffer Using the Atmospheric scanning Electron Microscope (ASEM)2011

    • Author(s)
      佐藤主税
    • Organizer
      MICROSCOPY & MICROANALYSIS 2011
    • Place of Presentation
      Nashville Convention Center (Nashville, USA)
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Rapid Observation with an Atmospheric Scanning Electron Microscope2011

    • Author(s)
      佐藤主税
    • Organizer
      MICROSCOPY & MICROANALYSIS 2011
    • Place of Presentation
      Nashville Convention Center (Nashvill, USA)
    • Year and Date
      2011-08-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] タンパク結晶化への大気圧走査電子顕微鏡ASEMの応用2011

    • Author(s)
      佐藤主税
    • Organizer
      第11回日本蛋白質科学会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Year and Date
      2011-06-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大気圧電子顕微鏡ASEMによる迅速な水中免疫電顕法2011

    • Author(s)
      佐藤主税
    • Organizer
      日本電子顕微鏡学会第67回学術講演会
    • Place of Presentation
      九州大学病院キャンパス(福岡県)
    • Year and Date
      2011-05-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Protein complex analyses using single particle reconstruction and atmos pheric scanning electron microscope (ASEM)2010

    • Author(s)
      佐藤主税
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Depiction of 3D structure and assignment of functional subunits of membrane proteins using a transmission electron microscopy2010

    • Author(s)
      K.Mio
    • Organizer
      15th Congress on Advances in Oncology and 13th International Symposium on Molecular Medicine
    • Place of Presentation
      Loutraki, Greese
    • Year and Date
      2010-10-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of oxidative stress sensor Keap 1 protein using single particle reconstruction and ASEM2010

    • Author(s)
      C.Sato
    • Organizer
      15th Congress on Advances in Oncology and 13th International Symposium on Molecular Medicine
    • Place of Presentation
      Loutraki, Greese
    • Year and Date
      2010-10-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Protein complexes analysis using single particle reconstruction and SEM2010

    • Author(s)
      佐藤主税
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 透過電顕と走査電顕を用いたタンパク質・細胞のイメージング法の開発2010

    • Author(s)
      佐藤主税
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] The New Atmospheric Scanning Electron Microscope observes cells in solution as an optical- and electron-correlative microscope2010

    • Author(s)
      M.Suga
    • Organizer
      52nd Symposium of the Society for Histochemistry
    • Place of Presentation
      Prague
    • Year and Date
      2010-09-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Observation of Cells with a New Atmospheric Scanning Electron Microscope2010

    • Author(s)
      寺本華奈江
    • Organizer
      52nd Symposium of the Society for Histochemistry
    • Place of Presentation
      Prague
    • Year and Date
      2010-09-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] TRPM2のクライオ単粒子解析とTRP関連タンパク質のASEM水中免疫電顕2010

    • Author(s)
      丸山雄介
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2010-06-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大気圧SEMによるバクテリア観察2010

    • Author(s)
      寺本華奈江
    • Organizer
      日本顕微鏡学会 第66回学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大気圧下での動的観察を可能にする走査電子顕微鏡2010

    • Author(s)
      西山英利
    • Organizer
      日本顕微鏡学会 第66回学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大気圧走査電子顕微鏡ASEMによる生体分子の観察2010

    • Author(s)
      佐藤主税
    • Organizer
      日本顕微鏡学会 第66回学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大気圧走査電子顕微鏡(ClairScope)による画像とそのコントラスト形成機構2010

    • Author(s)
      須賀三雄
    • Organizer
      日本顕微鏡学会 第66回学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2010-05-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大気圧電子顕微鏡ASEMによる水中の試料の観察法:迅速免疫電顕法

    • Author(s)
      海老原 達彦,西山 英利,須賀 三雄,佐藤 主税
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府吹田市千里万博公園1-5)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 大気圧走査電子顕微鏡

    • Author(s)
      佐藤主税,西山英利,海老原達彦,須賀三雄
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府吹田市千里万博公園1-5)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] The atmospheric scanning electron microscope (ASEM) observes cells and tissues in open solution: Wet Immuno-EM

    • Author(s)
      T.Ebihara, H.Nishiyama, M.Suga, C.Sato
    • Organizer
      日本組織培養学会 第86回大会
    • Place of Presentation
      産業技術総合研究所 つくばセンター (茨城県つくば市東1-1-1)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 透過型電子顕微鏡を用いた三次元再構成法によって明らかにされたTRPC5イオンチャネルの構造

    • Author(s)
      Memtily Nassirhadjy,三尾和弘,小椋俊彦,清中茂樹、森泰夫,佐藤主税
    • Organizer
      第9回TRPチャネル研究会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市明大寺町伝馬8-1)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 水中を観察できる新型電子顕微鏡ASEMを用いたマウスの研究:神経細胞初代培養やイオンチャンネルを中心として

    • Author(s)
      海老原 達彦,西山 英利,佐藤 真理,須賀 三雄,佐藤 主税
    • Organizer
      第27回モロシヌス研究会
    • Place of Presentation
      理化学研究所バイオリソースセンター・森脇和郎ホール(茨城県つくば市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 3D transmission electron microscopy of membrane proteins and direct observation of cells, tissues and crystals in solution using ASEM

    • Author(s)
      C.Sato, M.Kawata, H.Nishiyama, M.Suga, M.Sato,T.Senda, T.Ebihara, K.Mio
    • Organizer
      ICSG2013-SLS
    • Place of Presentation
      京王プラザホテル札幌(札幌市中央区北5条西7-2-1)
    • Related Report
      2013 Annual Research Report
  • [Presentation] The Atmospheric Scanning Electron Microscope (ASEM) Observes the Critical Moment of Platelet Generation from Megakaryocytes in Solution

    • Author(s)
      C.Sato, H.Nishiyama, Y.Maruyama, T.Ebihara, M.Suga, M.Yamamoto, H.Motohashi
    • Organizer
      M&M2013
    • Place of Presentation
      Indiana Convention Center(Indianapolis, USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 水中が見れる大気圧走査電子顕微鏡(ASEM)の臨床診断化にむけて

    • Author(s)
      佐藤主税
    • Organizer
      第757回千葉県がんセンター研究所集談会
    • Place of Presentation
      千葉県がんセンター(千葉市中央区仁戸名町666-2)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 大気圧電子顕微鏡(ASEM)によるタンパク質微結晶と細胞内複合体の液中観察

    • Author(s)
      海老原 達彦,川田 正晃,西山英利,千田美紀,佐藤 真理,須賀三雄,千田俊哉,佐藤 主税
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館(京都市左京区宝ヶ池)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大気圧走査電子顕微鏡による水中での細胞の観察:乳酸菌と細胞外構造

    • Author(s)
      杉本真也,奥田賢一,東山堅一,並木健西山英利,須賀三雄,松本俊介,園元謙二,水之江義充,佐藤 主税
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(神戸市中央区港島中町6-11-1)
    • Related Report
      2013 Annual Research Report
  • [Book] Methods in Cell Biology Volume 111 Correlative Light and Electron Microscopy.2012

    • Author(s)
      Ian E.G.Morrison
    • Publisher
      Academic Press
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://staff.aist.go.jp/ti-sato/index.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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