| Budget Amount *help |
¥194,350,000 (Direct Cost: ¥149,500,000、Indirect Cost: ¥44,850,000)
Fiscal Year 2014: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
Fiscal Year 2013: ¥30,550,000 (Direct Cost: ¥23,500,000、Indirect Cost: ¥7,050,000)
Fiscal Year 2012: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2011: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
Fiscal Year 2010: ¥70,980,000 (Direct Cost: ¥54,600,000、Indirect Cost: ¥16,380,000)
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| Outline of Final Research Achievements |
Nucleosomes are the fundamental repeating units of chromatin. Nucleosomes must be disassembled and reassembled during nuclear reactions, such as replication, transcription, repair, and recombination. During the reassembly of nucleosomes, histones need to be deposited on genomic DNA in the correct procedure. If not, epigenetic information, which is encoded on the N-tail of histones, is eliminated in the course of nuclear reaction coupled nucleosome reassembly. Here, we focused on the histone deposition factors, CAF-I and HIRA. We established a protocol for the large-scale purification of CAF-1 (a replication-dependent histone deposition factor), which enabled us to obtain images from EM analyses. Regarding HIRA, which is a replication-independent histone deposition factor, we have obtained crystals of the HIRA-C domain. We will now try crystal optimization. In addition, we have determined the crystal structure of CagA, which has been implicated in Helicobacter pylori pathogenicity.
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