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Deciphering mechanism underlying neuro-vascular wiring by live-imaging using zebrafish

Planned Research

Project AreaVasculo-neural wiring and their interdependent crosstalk
Project/Area Number 22122003
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

MOCHIZUKI Naoki  独立行政法人国立循環器病研究センター, 研究所, 部長 (30311426)

Co-Investigator(Kenkyū-buntansha) KOICHI Miura  国立循環器病研究センター, 研究所, 研究員 (20360349)
HIROYUKI Nakajima  国立循環器病研究センター, 研究所, 研究員 (10467657)
HAJIME Fukui  国立循環器病研究センター, 研究所, 研究員 (80551506)
HARUKO Takano  国立循環器病研究センター, 研究所, 研究員 (40532891)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥118,040,000 (Direct Cost: ¥90,800,000、Indirect Cost: ¥27,240,000)
Fiscal Year 2014: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
Fiscal Year 2013: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2012: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2011: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2010: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Keywordsイメージング / 血管 / 神経 / 伴走 / ゼブラフィッシュ / 交感神経 / 脂肪 / ワイヤリング / VEGF
Outline of Final Research Achievements

Physiologocal homeostasis of human bodies is mainly regulated by the neural system and the vascular system delivering endocrine hormones. Both systems are consisted of circuits between central to periphery and vice versa. Therefore, neurons and blood vessels are found to exist side by side in certain anatomical regions.
We tried to search for neuro-vascular wiring system in vivo using zebrafish embryos and identified several regions where blood vessels and neurons exhibit parallel growth during embryogenesis. In addition, we demonstrate a mechanism how artery regulate the parallel growth of motor neurons.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (29 results)

All 2015 2014 2013 2012 2011 2010 2001 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Invited: 4 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Recent advances in understanding of cardiovascular development by imaging of zebrafish.2015

    • Author(s)
      Fukuhara S, Fukui H, Wakayama Y, Ando K, Nakajima H, Mochizuki N.
    • Journal Title

      Dev Growth Differ.

      Volume: 未定 Issue: 4 Pages: 109-122

    • DOI

      10.1111/dgd.12210

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] β-Catenin-dependent transcription is central to Bmp-mediated formation of venous vessels.2015

    • Author(s)
      Kashiwada T, Fukuhara S (Co-corresponding author), Terai K, Tanaka T, Wakayama Y, Ando K, Nakajima H, Fukui H, Yuge S, Saito Y, Gemma A, Mochizuki N.
    • Journal Title

      Development

      Volume: 142 Pages: 497-509

    • DOI

      10.1242/dev.115576

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Visualizing the cell-cycle progression of endothelial cells in zebrafish.2014

    • Author(s)
      Fukuhara S (Co-corresponding author), Zhang J, Yuge S, Ando K, Wakayama Y, Sakaue-Sawano A, Miyawaki A, Mochizuki N.
    • Journal Title

      Dev. Biol.

      Volume: 393 Issue: 1 Pages: 10-23

    • DOI

      10.1016/j.ydbio.2014.06.015

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish.2013

    • Author(s)
      Kwon HB, Fukuhara S, Asakawa K, Ando K, Kashiwada T, Kawakami K, Hibi M, Kwon YG, Kim KW, Alitalo K, Mochizuki N.
    • Journal Title

      Development

      Volume: 140 Pages: 4081-4090

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rap1 potentiates endothelial cell junctions by spatially controlling myosin II activity and actin organization.2013

    • Author(s)
      Ando K, Fukuhara S, Moriya T, Obara Y, Nakahata N, Mochizuki N.
    • Journal Title

      J Cell Biol.

      Volume: 202 Pages: 901-916

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Angiopoietin-1 Guides Directional Angiogenesis Through Integrin αvβ5 Signaling for Recovery of Ischemic Retinopathy.2013

    • Author(s)
      Lee J, Kim KE, Choi DK, Jang JY, Jung JJ, Kiyonari H, Shioi G, Chang W, Suda T, Mochizuki N, Nakaoka Y, Komuro I, Yoo OJ, Koh GY
    • Journal Title

      Sci Transl Med.

      Volume: 18

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of EphA2-mediated tyrosine phosphorylation of Shp2 in Shp2-regulated activation of extracellular signal-regulated kinase2013

    • Author(s)
      Miura, K.
    • Journal Title

      Oncogene

      Volume: 32 Issue: 45 Pages: 5292-5301

    • DOI

      10.1038/onc.2012.571

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The sphingosine-1-phosphate transporter Spns2 expressed on endothelial cells regulates lymphocyte trafficking in mice.2012

    • Author(s)
      Fukuhara S, Simmons S, Kawamura S, Inoue A,Tokudome T, Sunden Y, Arai Y, Moriwaki K, Ishida J, Uemura A, Kiyonari H, Abe T, Fukamizu A, Hirashima M, Sawa H, Aoki J, Ishii M, Mochizuki N
    • Journal Title

      J. Clin. Invest.

      Volume: 122 Pages: 1416-1426

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EphrinA/EphA signal facilitates insulin-like growth factor-I-induced myogenie differentiation through suppression of the Ras/extracellular signal-regulated kinase 1/2 cascade in myoblast cell lines2011

    • Author(s)
      Minami M
    • Journal Title

      Mol.Biol.Cell

      Volume: 22 Pages: 3508-3519

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Angiopoietin-1/Tie2 Signal Augments Basal Notch Signal Controlling Vascular Quiescence by Inducing Delta-Like 4 Expression through AKT-mediated Activation of {beta}-Catenin2011

    • Author(s)
      Zhang J
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Pages: 8055-8066

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calpain protects the heart from hemodynamic stress2011

    • Author(s)
      Taneike M
    • Journal Title

      J Biol.Chem.

      Volume: 286 Pages: 32170-32177

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Docking Protein Gab1 Is an Essential Component of Postnatal Angiogenesis After Ischemia via HGF/c-Met Signaling2011

    • Author(s)
      Shioyama W
    • Journal Title

      Circ.Res.

      Volume: 108 Pages: 664-675

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AMPK controls the speed of microtubule polymerization and directional cell migration through CLIP-170 phosphorylation.2010

    • Author(s)
      Nakano A
    • Journal Title

      Nat.Cell Biol

      Volume: 12 Pages: 583-590

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vascular Endothelial-Cadherin Stabilizes at Cell-Cell Junctions by Anchoring to Circumferential Actin Bundles through α- and β-Catenins in Cyclic AMP-Epac-Rap1 Signal-activated Endothelial Cells.2010

    • Author(s)
      Noda K
    • Journal Title

      Mol.Biol.Cell

      Volume: 21 Pages: 584-596

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EphrinA1-EphA2 signal induces compaction and polarization of MDCK cells by inactivating ezrin through negative regulation of RhoA2001

    • Author(s)
      Wakayama Y
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Pages: 44243-44253

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] In vivo imaging of YAP dynamics in zebrafish vascular development2014

    • Author(s)
      Hiroyuki Nakajima, Sigetomo Fukuhara, Naoki Mochizuki
    • Organizer
      The 18th International Vascular Biology Meeting (IVBM2014)
    • Place of Presentation
      京都メッセ
    • Year and Date
      2014-04-14 – 2014-04-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] A small GTPase Rap1 potentiates vascular endothelial barrier function through actin rearrangement by spatially controlling the myosin II activity2014

    • Author(s)
      Koji Ando, Shigetomo Fukuhara, Takahiro Moriya, Naoki Mochizuki
    • Organizer
      The 18th International Vascular Biology Meeting (IVBM2014)
    • Place of Presentation
      京都メッセ
    • Year and Date
      2014-04-14 – 2014-04-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] イメージングによる心臓・血管発生メカニズムの解明2013

    • Author(s)
      望月直樹
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      タワーホール船堀、東京
    • Year and Date
      2013-03-27
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] in vivo imaging of signal transduction pathways involved in angiogenesis2012

    • Author(s)
      Shigetomo Fukuhara (Naoki Mochizuki)
    • Organizer
      The 20th annual meeting of the Japanese vascular biology and medicine organization
    • Place of Presentation
      Awagin hall, Tokushima
    • Year and Date
      2012-12-05
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Functions of Spns2 a sphingosine 1-phosphate transporter, in zebra fish and mammals2011

    • Author(s)
      望月直樹
    • Organizer
      The second pacific symposium on vascular Biology
    • Place of Presentation
      Korea
    • Year and Date
      2011-11-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Imaging of cardiovasvular cells entering into cell cycle to dissect thesignaling n vascular quiescenceand cardiac regeneration2011

    • Author(s)
      望月直樹
    • Organizer
      The 8^<th> International Duodecim Symposium "Endothelial growth factors in cancer and cardiovascular diseases"
    • Place of Presentation
      Finland
    • Year and Date
      2011-06-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Angiopoietin-1/Tie2-mediated vascular maturation and quiescence are reregulate by Dll4-Notch signaling2010

    • Author(s)
      Naoki Mochizuki
    • Organizer
      Kloster Seeon Angiogenesis Meeting 2010
    • Place of Presentation
      Kloster Seeon, Germany
    • Related Report
      2010 Annual Research Report
  • [Presentation] ゼブラフィッシュを用いた新たな心筋細胞増殖機構の解明

    • Author(s)
      望月直樹
    • Organizer
      第36回分子生物学会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] In vivo imaging技術の進歩

    • Author(s)
      Naoki Mochizuki
    • Organizer
      第53回日本呼吸器学会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 国立循環器病研究センター研究所細胞生物学部、大阪大学医学系研究科連携大学院循環微小画像医学講座

    • URL

      http://plaza.umin.ac.jp/ncvc/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 独立行政法人国立循環器病研究センタ―研究所細胞生物学部

    • URL

      http://plaza.umin.ac.jp/ncvc/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://plaza.umin.ac.jp/ncvc/

    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 心筋細胞増殖誘導剤及びこれを含有する心筋梗塞治療用医薬組成物、並びに心筋細胞の製造方法2013

    • Inventor(s)
      望月直樹
    • Industrial Property Rights Holder
      独立行政法人 国立循環器病研究センター、他
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-198591
    • Filing Date
      2013-09-25
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] スフィンゴシン1-リン酸トランスポーターの新規機能2011

    • Inventor(s)
      望月直樹
    • Industrial Property Rights Holder
      国立循環器病研究センター
    • Industrial Property Number
      2011-198886
    • Filing Date
      2011-09-12
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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