| Budget Amount *help |
¥125,580,000 (Direct Cost: ¥96,600,000、Indirect Cost: ¥28,980,000)
Fiscal Year 2014: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2012: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2011: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2010: ¥43,420,000 (Direct Cost: ¥33,400,000、Indirect Cost: ¥10,020,000)
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| Outline of Final Research Achievements |
We investigated the effects of RECK on brain development and cellular behaviors in multiple experimental systems in vivo and in vitro. In vivo, we found that selective inactivation of Reck in vascular endothelial cells in mice results in perinatal death with severe disruption of tissue structure in the cerebral neocortex. Mice with selective inactivation of Reck in neural precursor cells, on the other hand, were found to be viable and show no obvious phenotypes. Close investigations, however, revealed that these mice had altered neocortical cytoarchitecture and show interesting behavioral phenotypes, such as anxiety-like and perseverative behaviors and reduced pre-pulse inhibition. In vitro, RECK was found to suppress cell cycle progression by downregulating SKP2, to be induced after epithelial-mesenchymal transition in normal cells but not in cancer cells, and to suppress fibrosis by inhibiting migration of cardiac fibroblasts.
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