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Studies on diversity of axon guidance mechanisms

Planned Research

Project AreaNeural Diversity and Neocortical Organization
Project/Area Number 22123006
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Tsukuba

Principal Investigator

MASU Masayuki  筑波大学, 医学医療系, 教授 (20243032)

Co-Investigator(Kenkyū-buntansha) SHIOMI Kensuke  筑波大学, 医学医療系, 講師 (00311598)
MASU Kazuko  筑波大学, 医学医療系, 講師 (50344883)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥104,780,000 (Direct Cost: ¥80,600,000、Indirect Cost: ¥24,180,000)
Fiscal Year 2014: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2012: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2010: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Keywords脳 / 発生 / 糖鎖 / 神経回路 / 大脳皮質 / ヘパラン硫酸 / 軸索ガイダンス / 皮質脊髄路 / 神経軸索 / スルファターゼ / 脳・神経 / 遺伝子 / 発生・分化
Outline of Final Research Achievements

We studied the roles of heparan sulfate in corticospinal tract formation. We generated the double knockout (DKO) mice for Sulf1 and Sulf2, which are normally required for 6-O-desulfation of heparan sulfate. We found that Sulf1/2 DKO mice had axon guidance defects in the corticospinal tract. The cortical axons abnormally projected towards the superior and inferior colliculi, and midline crossing did not occur normally in the pyramidal decussation. These results suggest that heparan sulfate modification by Sulf1/2 is required for normal corticospinal tract formation.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(43 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Open Access: 1 results) Presentation (21 results) (of which Invited: 1 results) Book (3 results) Remarks (4 results)

  • [Journal Article] Expression of the heparan sulfate 6-0-endosulfatases, Sulfl and Sulf2, in the avian and mammalian inner earsuggests a role for sulfation during inner ear development.2015

    • Author(s)
      Freeman SD, Keino-Masu K, Masu M, Ladher RK.
    • Journal Title

      Dev Dyn

      Volume: 244 Pages: 168-180

    • DOI

      10.1002/dvdy.24223

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ErbB2 dephosphorylation and anti-proliferative effects of neuregulin-1 in ErbB2-overexpressing cell lines; re-evaluation of their low-affinity interaction.2013

    • Author(s)
      Wang, R., Iwakura, Y., Araki, K., Keino-Masu, K., Masu, M., Wang, X., Takei, N., Higashiyama, S. and Nawa, H.
    • Journal Title

      Scientific Reports

      Volume: 3 Pages: 1402-1402

    • DOI

      10.1038/srep01402

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A unique mouse model for investigating the properties of amyotrophic lateral sclerosis-associated protein TDP-43, by in utero electroporation.2013

    • Author(s)
      Akamatsu, M., Takuma, H., Yamashita, T., Okada, T., Keino-Masu, K., Ishii, K., Kwak, S., Masu, M., and Tamaoka, A.
    • Journal Title

      Neurosci Res.

      Volume: 77 Pages: 234-241

    • DOI

      10.1016/j.neures.2013.09.009

    • NAID

      120007130577

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヘパラン硫酸微細構造の組織特異性とその決定機2013

    • Author(s)
      桝和子、桝正幸
    • Journal Title

      医学のあゆみ

      Volume: 245 (7) Pages: 600-602

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organ-Specific Sulfation Patterns of Heparan Sulfate Generated by Extracellular Sulfatases Sulf1 and Sulf2 in Mice.2012

    • Author(s)
      Nagamine, S., Tamba, M., Ishimine, H., Araki, K., Shiomi, K., Okada, T., Ohto, T., Kunita, S., Takahashi, S., Wismans, R.G.P., van Kuppevelt, T.H., Masu, M., and Keino-Masu, K.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Pages: 9579-9590

    • DOI

      10.1074/jbc.m111.290262

    • NAID

      120007130630

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autotaxin expression from synovial fibroblasts is essential for the pathogenesis of modeled arthritis.2012

    • Author(s)
      Nikitopoulou, I., Oikonomou, N., Karouzakis, E., Sevastou, I., Nikolaidou-Katsaridou, N., Zhao, Z., Mersinias, V., Armaka, M., Xu, Y., Masu, M., Mills, G. B., Gay, S., Aidinis, V.
    • Journal Title

      Journal of Experimental Medicine

      Volume: 209 Pages: 925-933

    • DOI

      10.1084/jem.20112012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organ-Specific Sulfation Patterns of Heparan Sulfate Generated by Extracellular Sulfatases Sulf1 and Sulf2 in Mice2012

    • Author(s)
      Nagamine, S., Tamba, M., Ishimine, H., Araki, K., Shiomi, K., Okada, T., Ohto, T., Kunita, S., Takahashi, S., Wismans, R.G.P., van Kuppevelt, T.H., Masu, M., Kazuko Keino-Masu
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Pages: 9579-9590

    • NAID

      120007130630

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autotaxin expression from synovial fibroblasts is essential for the pathogenesis of modeled arthritis2012

    • Author(s)
      Nikitopoulou, I., Oikonomou, N., Karouzakis, E., Sevastou, I., Nikolaidou-Katsaridou, N., Zhao, Z., Mersinias, V., Armaka, M., Xu, Y., Masu, M., Mills, G.B., Gay, S., Kollias, G., Vassilis Aidinis
    • Journal Title

      Journal of Experimental Medicine

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development2011

    • Author(s)
      Koike, S., Yutoh, Y., Keino-Masu, K., Noji, S., Masu, M., Ohuchi, H.
    • Journal Title

      Developmental Dynamics

      Volume: 240 Pages: 413-421

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallographic characterization of the DIX domain of the Wnt signalling positive regulator Ccd12011

    • Author(s)
      Terawaki, S., Yano, K., Katsutani, T., Shiomi, K., Keino-Masu, K., Masu, M., Shomura, Y., Komori, H., Shibata, N., Higuchi, Y.
    • Journal Title

      Acta Crystallographica Section F

      Volume: 67 Pages: 758-761

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired vascular remodeling in the yolk sac of embryos deficient in ROCK-I and ROCK-II2011

    • Author(s)
      Kamijo, H., Matsumura, Y., Thumkeo, D., Koike, S., Masu, M., Shimizu, Y., Ishizaki, T., Narumiya, S.
    • Journal Title

      Genes to Cells

      Volume: 16 Pages: 1012-1021

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development.2011

    • Author(s)
      Koike, S., Yutoh, Y., Keino-Masu, K., Noji, S., Masu, M., Ohuchi, H.
    • Journal Title

      Dev.Dyn.

      Volume: 240 Pages: 413-421

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteolytic cleavage of the rat heparan sulfate 6-O-endosulfatase SulfFP2 by furin-type proprotein convertases.2010

    • Author(s)
      Nagamine, S., Keino-Masu, K., Shiomi, K., Masu, M.
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 391 Pages: 107-112

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of autotaxin/lysophospholipase D results in head cavity formation in mouse embryos through the LPA receptor-Rho-ROCK pathway.2010

    • Author(s)
      Koike, S., Keino-Masu, K., Masu, M.
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 400 Pages: 66-71

    • NAID

      120007137917

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combining the Cell Encapsulation Technique and Axon Guidance for Cell Transplantation Therapy.2010

    • Author(s)
      Yamazoe, H., Keino-Masu, K., Masu, M.
    • Journal Title

      J.Biomaterial Sci.

      Volume: 21 Pages: 1815-1826

    • NAID

      120007137895

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ゼブラフィッシュCCD1 DIXドメインのX線結晶解析X-ray crystallographic analysis of zebrafish CCD1 DIX domain2015

    • Author(s)
      藤田祥平、寺脇慎一、塩見健輔、桝和子、桝正幸、若松馨、柴田直樹、樋口芳樹
    • Organizer
      第32回PFシンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-03-17 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of the corticospinal tract formation in adult Sulf1;Sulf2 double knockout mice2014

    • Author(s)
      Takuya Okada, Nana Kagaya, Kazuko Keino-Masu, Masayuki Masu
    • Organizer
      第37回日本神経科学学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-11 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Electron microscopic observation of intranuclear aggregation of TDP-43 in mouse cerebral cortex produced by in utero electroporation2014

    • Author(s)
      Megumi Akamatsu, Hiroshi Takuma, Takenari Yamashita, Takuya Okada, Kazuko Keino-Masu, Hartmut Oehring, Shin Kwak, Masayuki Masu, Gustav F Jirikowski, Akira Tamaoka
    • Organizer
      第37回日本神経科学学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-11 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 子宮内電気穿孔法TDP-43 遺伝子導入によるin vivo形成封入体の微細形態の検討2014

    • Author(s)
      詫間浩、赤松恵、山下雄也、Oehring, H.、岡田拓也、桝和子、石井一弘、Jirikowski, G、郭伸、桝正幸、玉岡晃
    • Organizer
      第55回日本神経学会総会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2014-05-21 – 2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Heparan sulfate modification by endosulfatases Sulf1 and Sulf2 is required for the cotricospinal tract formation2014

    • Author(s)
      Kazuko Keino-Masu, Takuya Okada, Masayuki Masu.
    • Organizer
      International Symposium on Glyco-Neuroscience
    • Place of Presentation
      Awaji Yumebutai International Conference Center(Hyogo)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヘパラン硫酸エンドスルファターゼSulf1の嗅覚記憶における役割2013

    • Author(s)
      桝和子、奥谷文乃、椛秀人、桝正幸
    • Organizer
      第36回日本神経科学学会大会・第56回日本神経化学会大会・第30回日本神経回路学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス胎仔電気穿孔法による in vivo TDP-43 遺伝子導入と封入体形成の検討2013

    • Author(s)
      詫間浩、赤松恵、山下雄也、岡田拓也、石井一弘、桝和子、郭伸、桝正幸、玉岡晃
    • Organizer
      第54回日本神経学会総会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋萎縮性側索硬化症関連タンパク:TDP-43封入体の形成-マウス胎児電気穿孔法による大脳皮質運動野への疾患関連遺伝子の導入2013

    • Author(s)
      赤松恵、詫間浩、山下雄也、岡田拓也、石井一弘、桝和子、郭伸、桝正幸、玉岡晃
    • Organizer
      第36回日本神経科学学会大会・第56回日本神経化学会大会・第30回日本神経回路学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ccd1/Dixdc1欠損マウスにおける行動異常2013

    • Author(s)
      桝正幸、昌子浩孝、塩見健輔、宮川剛、桝和子
    • Organizer
      第36回日本神経科学学会大会・第56回日本神経化学会大会・第30回日本神経回路学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Extracellular sulfatases regulate PDGF signaling and promotes nephropathy in diabetes.2013

    • Author(s)
      Takashima, Y., Yashiro, H., Masu, M. Keino-Masu, K., and Nagata, M.
    • Organizer
      Kidney week 2012
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Role of podocyte extracellular sulfatase in regulation of glomerular permeability.2012

    • Author(s)
      Takashima, Y., Yamashita, H., Sakamoto, K., Hara, S., Kobayashi, N., Ueno, T., Keino-Masu, K., Masu, M. and Nagata, M.
    • Organizer
      Kidney week 2012
    • Place of Presentation
      San Diego Convention Center(米国)
    • Year and Date
      2012-10-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] Heparan Sulfate Modification by Endosulfatases is Required for Cortical Axon Guidance2012

    • Author(s)
      Masayuki Masu, Takuya Okada, Satoshi Nagamine, Tatsuyuki Ohto, Fuyuki Kametani, Masato Hasegawa, Satoshi Kunita, Satoru Takahashi, Kazuko Keino-Masu
    • Organizer
      1^<st> international symposium/59^<th> NIBB conferences "Neocortical organization"
    • Place of Presentation
      岡崎カンファレンスセンター(岡崎)(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヘパラン硫酸糖鎖による軸索ガイダンス制御機構2011

    • Author(s)
      桝正幸、岡田拓也、長嶺聖史、大戸達之、亀谷富由樹、長谷川成人、國田智、高橋智、桝和子
    • Organizer
      第34回日本神経科学学会大会
    • Place of Presentation
      パシフィコ横浜(横浜)(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] The roles of heparan sulfate in axon guidance2010

    • Author(s)
      桝正幸
    • Organizer
      International Graduate Schools International Conference
    • Place of Presentation
      つくば国際会議場(茨城)
    • Year and Date
      2010-11-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Wntシグナル伝達機構ではたらくCCD1の構造化学的研究2010

    • Author(s)
      寺脇慎一、塩見健輔、桝正幸、柴田直樹、樋口芳樹
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス胎児脳におけるヘパラン硫酸多様性の検出2010

    • Author(s)
      岡田拓也、桝和子、長嶺聖史、國田智、高橋智、Arie Oosterhof, Toin Kuppevelt、桝正幸
    • Organizer
      第33回日本神経科学学会大会・第53回日本神経化学会大会・第20回日本神経回路学会大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Wntシグナル伝達で機能するCCD1オリゴマー形成機構に関する構造学的研究2010

    • Author(s)
      寺脇慎一、塩見健輔、桝正幸、柴田直樹、樋口芳樹
    • Organizer
      日本結晶学会2010年年会
    • Place of Presentation
      大阪大学コンベンションセンター(大阪)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Heparan sulfate 6-Oendosulfatases are required for neural network formation.2010

    • Author(s)
      T.Okada, K.Keino-Masu, S.Nagamine, S.Kunita, S.Takahashi, M.Masu
    • Organizer
      The 40th Annual meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, convenction center(米国)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Wntシグナル伝達で機能するCCD1 DIXドメインのX線結晶構造解析2010

    • Author(s)
      寺脇慎一、塩見健輔、桝正幸、柴田直樹、樋口芳樹
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際会議場(兵庫)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Autotaxin is required for anterior neural ridge formation, midbrain-hindbrain boundary patterning, and cranial neural tube closure.2010

    • Author(s)
      E.Tagiuchi, S.Koike, Y.Yutoh, K.Keino-Masu, S.Noji, M.Masu, H.Ohuchi.
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際会議場(兵庫)
    • Related Report
      2010 Annual Research Report
  • [Presentation] TDP-43 aggregation produced by In utero electroporation and its properties in the mouse motor neurons

    • Author(s)
      Akamatsu, M., Takuma, H., Yamashita, T., Okada, T., Ishii, K., Keino-Masu, K., Kwak, S., Masu, M., Tamaoka, A.
    • Organizer
      43th Annual Meeting of Society for Neuroscience
    • Place of Presentation
      San Diego Convention Center, San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Book] Cortical Development: Neural Diversity and Neocortical Organization2013

    • Author(s)
      Masu, M.
    • Total Pages
      320
    • Publisher
      Springer-Verlag
    • Related Report
      2013 Annual Research Report
  • [Book] Lysophospholipid Receptors: Signaling and Biochemistry.2013

    • Author(s)
      Masu, M., Koike, S., Okada, T., and Keino-Masu, K.
    • Total Pages
      812
    • Publisher
      John Wiley & Son Inc
    • Related Report
      2013 Annual Research Report
  • [Book] Lysophospholipid Receptors: Signaling and Biochemistry2013

    • Author(s)
      Masu, M., Koike, S., Okada, T., and Keino-Masu, K.
    • Publisher
      John Wiley Son Inc.
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/duo/molneurobiol/

    • Related Report
      2014 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/duo/molneurobiol/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/duo/molneurobiol/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/basic-med/molneurobiol/index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-22   Modified: 2019-07-29  

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