| Budget Amount *help |
¥77,870,000 (Direct Cost: ¥59,900,000、Indirect Cost: ¥17,970,000)
Fiscal Year 2014: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2013: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2012: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2011: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2010: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
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| Outline of Final Research Achievements |
In this study, we aimed i) the establishment of ChIP-seq from very small number of cells, especially mouse preimplantation embyors, and ii) analysis of the (epi)genome reprogramming in fertilized 1-cell embryos using the new ChIP-seq technology.
In the former project, we obtained substantial improvement of reproducibility in top ~15% sequence reads by modifying the step of linear amplification of sequence libraries, although further modifications are still required to reach the goal. In the latter project, instead of using the new ChIP-seq method, we utilized recently identified histone mutants, which erase endogenous histone methylations at the mutated sites, to investigate the importance of histone methylations in preimplantation embryos. As a result, we identified that paternal-specific H3K4 monomethylation by Mll3/4 is required for transcription of paternal genome in late 1-cell embryos, likely through enhancer activation.
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