| Budget Amount *help |
¥88,400,000 (Direct Cost: ¥68,000,000、Indirect Cost: ¥20,400,000)
Fiscal Year 2014: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2013: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2012: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2011: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2010: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
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| Outline of Final Research Achievements |
The present study used Nesf (nesfatin) transgenic (Tg) mice to investigate the metabolic role of Nesf. No differences were observed in daily food intake and body weight. Nesf Tg mice showed decreased mRNA expression of CART. Nesf Tg mice fed 45% high-fat diet (HFD) showed higher increase in body weight than their non-Tg littermates; however, no difference was observed in daily food intake between these 2 groups. Nesf Tg mice fed 45% HFD showed a significant increase in the weight of the liver, subcutaneous fat, and brown adipose tissue and decrease in the expression of uncoupling protein-1 in the subcutaneous fat. Insulin levels of these Tg mice were significantly higher. Histological analysis showed marked fat deposition in the hepatocytes surrounding the hepatic central veins in Nesf/NUCB2 Tg mice fed 45% HFD. It is indicated that Nesf/NUCB2 is involved in the development of insulin resistance and fat deposition in the liver, independent of the modulation of energy intake.
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