|Budget Amount *help
¥182,780,000 (Direct Cost: ¥140,600,000、Indirect Cost: ¥42,180,000)
Fiscal Year 2014: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
Fiscal Year 2013: ¥30,550,000 (Direct Cost: ¥23,500,000、Indirect Cost: ¥7,050,000)
Fiscal Year 2012: ¥37,310,000 (Direct Cost: ¥28,700,000、Indirect Cost: ¥8,610,000)
Fiscal Year 2011: ¥36,920,000 (Direct Cost: ¥28,400,000、Indirect Cost: ¥8,520,000)
Fiscal Year 2010: ¥48,880,000 (Direct Cost: ¥37,600,000、Indirect Cost: ¥11,280,000)
|Outline of Final Research Achievements
Internal organs of animals often show directional Left-Right (LR) asymmetry. The LR asymmetric structure of the embryonic hindgut is formed through a 90 degree counterclockwise rotation. Before this rotation, hindgut epithelial cells show chirality in their shape. The hindgut rotation is driven by the transition from chiral to achiral cells. In this study, we tried to understand the mechanism how cell chirality drives the hindgut rotation.
We developed a method to measure the mechanical force that drives the hindgut rotation. Based on the measurement using this method, we showed that the hindgut rotation is driven by the active force generated by the hindgut itself. During this rotation, the hindgut cells slides and changes their relative position to the direction that is predicted from the rotation of hindgut tube. Our genetic mosaic analysis showed that cell chirality is formed in individual cells, which reflects their intrinsic chiral property.