| Budget Amount *help |
¥159,770,000 (Direct Cost: ¥122,900,000、Indirect Cost: ¥36,870,000)
Fiscal Year 2014: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥30,550,000 (Direct Cost: ¥23,500,000、Indirect Cost: ¥7,050,000)
Fiscal Year 2012: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2011: ¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000)
Fiscal Year 2010: ¥48,880,000 (Direct Cost: ¥37,600,000、Indirect Cost: ¥11,280,000)
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| Outline of Final Research Achievements |
In order to study the effects of mechanical force on the process of development, we need to establish a novel measurement method for stress distribution in an embryo at a micrometer scale. Such stress distribution can be estimated from topography and stiffness distribution of a cut surface of an embryo. In this study, we used Xenopus Laevis embryo as the test model, and have succeeded in developing a method to cut very soft tissue with Young’s modulus of ~10 Pa with minimal damage and to measure its surface topography within 30 s. Distribution of Young’s modulus was obtained by spraying small beads on the cut surface to measure their indentation into the embryonic tissue. We have found in gastrula that the embryonic surface is in tension especially around the blastopore and embryonic core is in compression, and that the level of stresses changes during the process of development.
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