Evolutionary mechanisms of novel complex evolutionary traits with small number of genes

• Project Area: Genetic bases for the evolution of complex adaptive traits
• Project/Area Number: 22128002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Hasebe Mitsuyasu 基礎生物学研究所, 生物進化研究部門, 教授 (40237996)
• Co-Investigator(Kenkyū-buntansha): OHSHIMA ISSEI 京都府立大学, 生命環境科学研究科, 助教 (50466455)
• Co-Investigator(Renkei-kenkyūsha): MURATA TAKASHI 基礎生物学研究所, 生物進化研究部門, 准教授 (00242024)
• Research Collaborator: HIWATASHI YUJI
• Project Period (FY): 2010-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥130,390,000 (Direct Cost: ¥100,300,000、Indirect Cost: ¥30,090,000); Fiscal Year 2014: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000); Fiscal Year 2013: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2012: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000); Fiscal Year 2011: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2010: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
• Keywords: 複合適応形質 / 進化 / 食虫植物 / フクロユキノシタ / 収斂 / クルミホソガ / 寄主転換 / 複合適応形質進化 / サラセニア / コモウセンゴケ / ホストレース転換 / オジギソウ / ホストレースチェンジ / 自己組織化 / 生活史進化 / ヒメツリガネゴケ / 陸上植物 / 分岐

Outline of Final Research Achievements

The theory of natural selection and the neutral theory of molecular evolution are powerful concepts in evolutionary biology. However, even with such theories, there still remain　unexplained phenomena, one of which is the evolution of complexity. We studied (1) evolution of Sarracenia purpurea pitcher leaves, (2) Genome sequence of Cephalotus follicularis and evolution of digestive enzymes, (3) Convergent evolution of digestive enzymes with adaptation to fluid environment, and so on.

Research Products

• [Int'l Joint Research] 成功大学(台湾)
• [Int'l Joint Research] ニューヨーク大学(米国)
• [Int'l Joint Research] Beijing Genome Institute(中国)
• [Int'l Joint Research] Ghent University(ベルギー)
• [Int'l Joint Research] Universitat de Barcelona(スペイン)
• [Int'l Joint Research]
• [Journal Article] Oriented cell division shapes carnivorous pitcher leaves of Sarracenia purpurea (2015)
  • Author(s): Fukushima K, Fujita H, Yamaguchi T, Kawaguchi M, Tsukaya H, Hasebe M.
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6-450
  • DOI: 10.1038/ncomms7450
  • Peer Reviewed / Open Access
• [Journal Article] Adaxial-abaxial polarity : The developmental basis of leaf shape diversity (2014)
  • Author(s): Fukushima K, Hasebe M.
  • Journal Title: Genesis Volume: 52 Issue: 1 Pages: 1-18
  • DOI: 10.1002/dvg.22728
  • Peer Reviewed / Open Access
• [Journal Article] Development of an Agrobacterium-Mediated Stable Transformation Method for the Sensitive Plant Mimosa pudica. (2014)
  • Author(s): Mano, H., Fujii, T., Sumikawa, N., Hiwatashi, Y., and Hasebe, M.
  • Journal Title: PLoS One Volume: 9 Issue: 2 Pages: e88611-e88611
  • DOI: 10.1371/journal.pone.0088611
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Contribution of NAC transcription factors to plant adaptation to land. (2014)
  • Author(s): Xu, B., Ohtani, M., Yamaguchi, M., Toyooka, K., Wakazaki, M., Sato, M., Kubo, M., Nakano, Y., Sano, R., Hiwatashi, Y., Murata, T., Kurata, T., Yoneda, A., Kato, K., Hasebe, M., and Demura, T.
  • Journal Title: Science Volume: 343 Issue: 6178 Pages: 1505-1508
  • DOI: 10.1126/science.1248417
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Early evolution of the vascular plant body plan - the missing mechanisms. (2014)
  • Author(s): Tomescu, A.M., Wyatt, S.E., Hasebe, M., and Rothwell, G.W.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 17C Pages: 126-136
  • DOI: 10.1016/j.pbi.2013.11.016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] KNOX2 genes regulate the haploid-to-diploid morphological transition in land plants (2013)
  • Author(s): Sakakibara, K., Ando, S., Yip, H.K., Tamada, Y., Hiwatashi, Y., Murata, T., Deguchi, H., Hasebe, M., and Bowman, J.L.
  • Journal Title: Science Volume: 339 Issue: 6123 Pages: 1067-1070
  • DOI: 10.1126/science.1230082
  • Peer Reviewed
• [Journal Article] AP2-type transcription factors determine stem cell identity in the moss Physcomitrella patens (2012)
  • Author(s): Aoyama, T., Hiwatashi, Y., Shigyo, M., Kofuji, R., Kubo, M., Ito, M., and Hasebe, M.
  • Journal Title: Development Volume: 139 Issue: 17 Pages: 3120-3129
  • DOI: 10.1242/dev.076091
  • Peer Reviewed
• [Journal Article] The gibberellin perception system evolved to regulate a pre-existing GAMYB-mediated system during land plant evolution (2011)
  • Author(s): Aya, K., Hiwatashi, Y., Kojima, M., Sakakibara, H., Ueguchi-Tanaka, M., Hasebe, M., Matsuoka, M.
  • Journal Title: Nature Communications Volume: 2 Issue: 1 Pages: 544-544
  • DOI: 10.1038/ncomms1552
  • Peer Reviewed
• [Journal Article] The Selaginella genome identifies genetic changes associated with the evolution of vascular plants (2011)
  • Author(s): Banks, et al
  • Journal Title: Science Volume: 332 Issue: 6032 Pages: 960-963
  • DOI: 10.1126/science.1203810
  • Peer Reviewed
• [Journal Article] Phylogeny, biogeography, and host-plant association in the subfamily Apaturinae (Insecta : Lepidoptera : Nymphalidae) inferred from eight nuclear and seven mitochondrial genes (2010)
  • Author(s): Ohshima, et al
  • Journal Title: Mol.Phylogenet.Evol. Volume: 57 Pages: 1026-1036
  • Peer Reviewed
• [Presentation] 独立に食虫植物化した分類群間に見られる消化酵素の共通性 (2012)
  • Author(s): 福島健児, 野澤昌文, 西山智明, 重信秀治, 住川直美, 牧野由美子, 長谷部光泰
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学（兵庫県神戸市）
• [Presentation] he genetic mechanisms of host race formation and maintenance (2011)
  • Author(s): Oshima I.
  • Organizer: 20th CDB Meeting
  • Place of Presentation: 理化学研究所(兵庫県)
  • Year and Date: 2011-02-23
• [Presentation] QTL解析による寄主転換の遺伝基盤の解明 (2011)
  • Author(s): 大島一正、長谷部光泰
  • Organizer: 日本進化学会第13回大会
  • Place of Presentation: 京都大学(京都)
• [Presentation] Unique adaxial-abaxial patterning and the evolutionary implication of pitcher-shaped leaves of Sarracenia purpurea (2011)
  • Author(s): Fukushima K., Yamaguchi T., Tsukaya H., Hasebe M.
  • Organizer: East Asian Botany : International Symposium 2011
  • Place of Presentation: 筑波大学(茨城県)
• [Presentation] Unique adaxial-abaxial patterning of pitcher-shaped leaves of the carnivorous plant Sarracenia purpurea (2011)
  • Author(s): Fukushima K., Hasebe M.
  • Organizer: Keystone Symposia : Evolutionary Developmental Biology
  • Place of Presentation: Granlibakken(アメリカ)
• [Presentation] QTL mapping of a larval-performance trait in the leaf mining moth Acrocercops transecta (2011)
  • Author(s): Oshima I., Hasebe M.
  • Organizer: Keystone Symposia : Evolutionary Developmental Biology
  • Place of Presentation: Granlibakken(アメリカ)
• [Presentation] 連鎖解析によるクルミホソガ幼虫の寄主適応遺伝子の探索 (2010)
  • Author(s): 大島一正、長谷部光泰
  • Organizer: 日本昆虫学会第70回大会
  • Place of Presentation: 山形大学(山形県)
  • Year and Date: 2010-09-20
• [Presentation] クルミホソガAcrocercops transectaにおける幼虫の寄主適応の遺伝基盤 (2010)
  • Author(s): 大島一正、長谷部光泰
  • Organizer: 日本進化学会第12回大会
  • Place of Presentation: 東京工業大学(東京都)
  • Year and Date: 2010-08-03
• [Presentation] QTL mapping of a larval-performance trait in the leaf mining moth Acrocercops transecta (2010)
  • Author(s): Oshima I., Hasebe M.
  • Organizer: Memorial Symposium for the 26th International Prize for Biology
  • Place of Presentation: つくば国際会議場(茨城県)
• [Presentation] 食虫植物における葉形態の複雑化メカニズム
  • Author(s): 福島健児, 長谷部光泰
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県福岡市）
• [Presentation] フクロユキノシタゲノムから迫る食虫植物の進化
  • Author(s): 福島健児, Likai Mao, 西山智明, 野澤昌文, 住川直美, 笠原雅弘, 柴田朋子, 重信秀治, Xiaodong Fang, 長谷部光泰
  • Organizer: 「生命情報科学若手の会」第４回研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
• [Presentation] 食虫植物における消化酵素遺伝子の進化
  • Author(s): 福島健児, 野澤昌文, 西山智明, 重信秀治, 住川直美, 長谷部光泰
  • Organizer: 日本植物分類学会第１２回大会
  • Place of Presentation: 千葉大学（ 千葉県千葉市）
• [Presentation] The genetics of host shifting in a leaf-mining moth Acrocercops transecrta (Gracillariidae)
  • Author(s): 大島一正
  • Organizer: 24th International Congress of Entomology
  • Place of Presentation: Daegu, Korea
• [Presentation] QTL解析とゲノム解析による寄主転換の遺伝基盤の解明
  • Author(s): 大島一正、長谷部光泰
  • Organizer: 日本応用動物昆虫学会第56回大会
  • Place of Presentation: 近畿大学（奈良）
  • Invited
• [Presentation] リーフマイナーを用いた遺伝学：寄主適応の遺伝基盤に迫る
  • Author(s): 大島一正
  • Organizer: 日本昆虫学会第71回大会
  • Place of Presentation: 信州大学(長野県松本市)
  • Invited
• [Presentation] The recurrent co-option in the evolution of digestive enzymes of carnivorous plants
  • Author(s): Fukushima, K. and M. Hasebe.
  • Organizer: 10th Korea-China-Japan Bioinformatics Training Course and Symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] 食虫植物ムラサキヘイシソウの捕虫葉における特異的な向背軸パターニング
  • Author(s): 福島健児, 山口貴大, 塚谷裕一, 長谷部光泰
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学(東京)
• [Presentation] 食虫植物を材料とした新奇形態進化機構の解明
  • Author(s): 福島健児, 山口貴大, 塚谷裕一, 長谷部光泰
  • Organizer: 日本生化学会第84回大会
  • Place of Presentation: 京都大学(京都)
• [Presentation] 食虫植物の消化酵素遺伝子における平行進化
  • Author(s): 福島健児, 野澤昌文, 西山智明, 重信秀治, 住川直美, 長谷部光泰
  • Organizer: 生命情報科学若手の会第３回研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
• [Presentation] 食虫植物から新奇形質の進化を探る
  • Author(s): 福島健児, 長谷部光泰
  • Organizer: 第43回種生物学シンポジウム
  • Place of Presentation: 一般財団法人人材開発センター富士研修所(山梨県富士吉田市)
• [Presentation] 食虫植物ムラサキヘイシソウにおける袋状捕虫葉の発生メカニズム
  • Author(s): 福島健児, 山口貴大, 塚谷裕一, 長谷部光泰
  • Organizer: 日本植物分類学会第11回大会
  • Place of Presentation: 大阪学院大学（大阪府吹田市）
• [Book] 野生生物を用いた実験系確立ストーリー．「種間関係の生物学」「リーフマイナーの食性の進化を探る」第3章 (p. 71-100) (2012)
  • Author(s): 大島一正
  • Total Pages: 399
  • Publisher: 文一総合出版
• [Remarks] http://www.nibb.ac.jp/evodevo
• [Remarks] 研究室web page
  • URL: http://www.nibb.ac.jp/evodevo
• [Remarks] 研究室web page
  • URL: http://www.nibb.ac.jp/evodovo
• [Remarks] 研究室webページ
  • URL: http://www.nibb.ac.jp/evodevo